CN101406131A - Method for cultivating root-knot nematode resistant eggplant - Google Patents
Method for cultivating root-knot nematode resistant eggplant Download PDFInfo
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- CN101406131A CN101406131A CNA2008101600958A CN200810160095A CN101406131A CN 101406131 A CN101406131 A CN 101406131A CN A2008101600958 A CNA2008101600958 A CN A2008101600958A CN 200810160095 A CN200810160095 A CN 200810160095A CN 101406131 A CN101406131 A CN 101406131A
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- eggplant
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- knot nematode
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Abstract
The invention relates to a method for cultivating a root-knot nematode-resistant eggplant plant. The method is to graft a plant of a conventionally cultivated highly root-knot nematode-sensitive eggplant variety serving as a scion onto a stramonium plant serving as a stock to obtain a graft plant, that is, the root-knot nematode-resistant eggplant plant. The root-knot nematode-resistant stramonium plant is adopted as the stock, the plant of the conventionally cultivated highly root-knot nematode-sensitive eggplant variety is adopted as the scion and the seedlings are grafted at the most proper position by the most proper method in a period of highest affinity, so the method can improve the inosculation of the two, greatly improve the survival rate of grafting by improving cultivation conditions. The grafted seedling has obvious prevention and cure effect on root-knot nematode and overcomes a continuous cropping obstacle, so the method enormously improves economic efficiency and, meanwhile, achieves obvious ecological benefits due to reduction in use of agricultural chemicals.
Description
Technical field
The present invention relates to a kind of method of cultivating anti-root knot line eggplant in the biological technical field, be used for the anti-root-knot nematode cultivation of eggplant.
Background technology
Eggplant is the principal item that protection ground vegetables produce; because continuous cropping cultivation for many years; various soil-borne diseases particularly root knot nematode disease be year by year the trend that rises, root knot nematode disease has become a kind of destructive disease of vegetables facility cultivation, often causes the serious underproduction even total crop failure.
The eggplant grafting not only can diseases prevention, also since stock than former scion well developed root system, suction, fertilizer sucting ability is strong, can significantly improve output, utilization has the good eggplant kind of the stock of high resistance and yielding ability to carry out grafting cultivation to multiple soil-borne disease, can effectively prevent the generation of soil-borne disease, overcome continuous cropping obstacle, obtain stable high yield.
Growth characteristics difference between the eggplant rootstock will be considered suitable grafting method during at a kind of stock and scion grafting, such as differences such as date of seeding, seedling ages, so that select excellently in excellent, makes its graft survival rate the highest, and grafting is the most strong, so that produce the highest income.
Summary of the invention
The technical problem to be solved in the present invention is at above problem, and a kind of method of cultivating anti-root nematode disease eggplant is provided, and this method can effectively prevent the generation of soil-borne diseases such as root-knot nematode, overcomes continuous cropping obstacle, obtains eggplant stable high yield.
For addressing the above problem, the present invention adopts following technical scheme: a kind of method of cultivating root-knot nematode resistant eggplant, this method is as stock with datura, the conventional cultivation eggplant kind of gaogangen nematode is as scion, carry out grafting and obtain grafting and be anti-root-knot nematode eggplant, realize through the following steps:
1) cultivates
A) with the datura seed in humidity 90%～95%, under 20～28 ℃ of conditions of temperature, stock presprouting of seeds 4～6 days, sowing and the stock strain is cultured to 5～7 true leaves then;
B) planting seed of datura described in the step a) is after 5～7 days, and the eggplant seed that will have gaogangen nematode is in humidity 90%～95%, under 20～28 ℃ of conditions of temperature, and stock presprouting of seeds 2～4 days, sowing and the scion strain is cultured to 4～6 true leaves then;
2) grafting
The stock strain that has 5～7 true leaves described in the step 1) is kept 2 true leaves, with step 2) described in have 4～6 true leaves the scion strain keep 2～3 true leaves and carry out grafting;
3) management
It is 25～28 ℃ that seedling after the grafting is placed on temperature, and humidity is to cultivate 7～10 days in the environment more than 85%;
4) hardening
After 7～10 days, original position is ventilated and is fallen wet hardening with the cultivation of the seedling after the grafting, and hardening can be carried out normal management after 3～5 days, continues to cultivate the eggplant grafting that obtains anti-root-knot nematode.
A kind of concrete prioritization scheme, step 2) grafting method described in is a cleft graft.
A kind of concrete prioritization scheme, the conventional cultivation eggplant kind of described gaogangen nematode is the long eggplant of peasant.
A kind of concrete prioritization scheme, the conventional cultivation eggplant kind of described gaogangen nematode is Bu Lita.
Beneficial effect: the present invention adopts technique scheme, have the following advantages: because the datura of employing root-knot nematode resistant is as stock, the conventional cultivation eggplant kind of gaogangen nematode is as scion, at the only position of young plant, use only method, when compatibility is the highest, carry out grafting, promoted the fusion of the two, and by improving breeding condition, the utmost point has improved the grafting survival rate significantly; Grafting has remarkable prevention effect in the field to root knot nematode disease, has overcome continuous cropping obstacle, has improved economic benefit significantly, owing to reduced the use of agricultural chemicals, has significant ecological benefits simultaneously.
Below in conjunction with drawings and Examples the present invention is elaborated.
Embodiment
Specifically set forth the present invention below in conjunction with example.
Embodiment one: as scion, as stock, cultivate anti-root knot line eggplant with datura with the long eggplant of the conventional cultivation eggplant kind peasant of gaogangen nematode, get over the cultivation of Xia Yanqiu stubble.
1, the incubation step of the sick eggplant of anti-root knot line is as follows:
1) on February 1st, 2007, datura (Datura Stramonium L.) planting seed is watered in matrix then, and making water content of substrate is 60%～80%, be put in 20～28 ℃ the seedling rearing room it is emerged, 5～7 true leaf strong sprouts that the stock strain is cultured to.
2) on February 6th, 2007, the long eggplant planting seed of peasant in matrix, is watered then, making water content of substrate is 60%～80%, is put in 20～28 ℃ the seedling rearing room it is emerged, 4～6 true leaf strong sprouts that the scion strain is cultured to.
Matrix is made up of careless carbon, vermiculite and perlite, and careless carbon, vermiculite and perlitic volume ratio are 3: 2: 1.
3) on March 5th, 2007, the beginning grafting.
A) grafting condition: the comprehensive thorough disinfection of grafting apparatus, the young plant grafting is watered the previous day, and the water content of stock strain reaches more than 75% when making grafting, and scion strain water content is 65%～75%, operating room's indoor temperature is between 25～30 ℃, and young plant does not have water droplet during grafting.
B) grafting: grafting method is a cleft graft, and step is as follows:
(1) the stock strain is fallen its head truncation at the 2nd true leaf place, keeps the bottom, with sterile blade the stem of stock strain is split vertically downward by the center of circle then and is cut 1～1.5cm.
(2) at the thick or thickness similar positions such as cutting part of scion strain, the scion strain is cut off, kept top, with blade its stem is whittled into 1～1.5cm chock then to stock.
(3) scion is inserted closely the position of riving of stock strain, folder is gone up plastic clip then.
4) management
It is 25～28 ℃ that seedling after the grafting is placed on temperature, and humidity is in the sealing plastic canopy more than 85%, to cultivate and after 7～10 days film is opened gradually, and original position is ventilated and fallen the humidity hardening, and hardening can be carried out normal management after 3～5 days, can transplant after 15 days.
Datura (Datura Stramonium L.) and grafting 2000 strains altogether of the long eggplant of peasant survive 1870 strains, and survival rate reaches 93.5%.
On April 2nd, 2007 was transplanted to datura (Datura Stramonium L.) and the long eggplant grafting of peasant in the serious greenhouse of root-knot nematode, and survival rate is 98.2%.
2, productivity of anti-root-knot nematode eggplant grafting and field performance
Field trial is in the vegetables base, and selected greenhouse by solar heat root knot nematode disease is very serious, and it is sponge gourd that preceding stubble is planted plant, owing to the harm of root-knot nematode is had no harvest.
Test and establish 2 processing, be respectively datura (Datura Stramonium L.) and long eggplant grafting and long eggplant own-rooted tree (in contrast), 4 repetitions are adopted district's group arrangement at random, sub-district area 50m
2, every sub-district field planting 120 strains.In identical environment and condition of culture, be cultured to fruit maturation.
3, the result shows
Since on May 15th, 2007, long eggplant own-rooted tree began to have the generation of the sick worm of a small amount of root knot line, and datura (Datura Stramonium L.) is not investigated root knot with long eggplant grafting; To on June 20th, 2007, long eggplant just reached 65.6% from the root knot index of root, and the root knot index of datura (Datura StramoniumL.) and long eggplant grafting is 1.97%; To on July 20th, 2007, the root knot index of long eggplant own-rooted tree reached highest level 100%, and the root knot index of datura (Datura Stramonium L.) and long eggplant grafting only is 2.99%, has only the root knot of minute quantity.Its output is respectively: datura (DaturaStramonium L.) is 1.55 * 10 with long eggplant grafting
5Kghm
-2, the long eggplant own-rooted tree of peasant is 1.03 * 10
5Kghm
-2, use datura (Datura Stramonium L.) and long eggplant grafting can increase output 5.2 * 10 than the long eggplant own-rooted tree of peasant
4Kghm
-2, amount of increase in production reaches 50.5%.
Wherein, the root knot index calculation method is as follows:
Carry out the root-knot nematode hazard rating according to following standard:
The onset grade symptom is described
0 grade: root is normal, does not have visible root knot
1 grade: on 1/3rd following roots a small amount of root knot is arranged
3 grades: on 1/3rd to 1/2nd root knot is arranged
5 grades: on 1/2nd above roots root knot is arranged, in a small amount of secondary root root knot takes place
7 grades: on all roots, comprise and also cover with root knot in the secondary root.
Embodiment two: as scion, as stock, cultivate anti-root knot line eggplant with datura with the conventional cultivation eggplant kind Bu Lita of gaogangen nematode, carry out Winter-Spring stubble cultivation.
Below test is carried out in a green food vegetable base:
1, the incubation step of the sick eggplant of anti-root knot line is as follows:
1) on October 15th, 2007, datura (Datura Stramonium L.) planting seed is watered in matrix then, and making water content of substrate is 60%～80%, be put in 20～28 ℃ the seedling rearing room it is emerged, 5～7 true leaf strong sprouts that the stock strain is cultured to.
2) on October 20th, 2007, the Bu Lita planting seed in matrix, is watered then, making water content of substrate is 60%～80%, is put in 20～28 ℃ the seedling rearing room it is emerged, 4～6 true leaf strong sprouts that the scion strain is cultured to.
Matrix is made up of careless carbon, vermiculite and perlite, and careless carbon, vermiculite and perlitic volume ratio are 3: 2: 1.
3) on December 6th, 2007, the beginning grafting.
A) grafting condition: the comprehensive thorough disinfection of grafting apparatus, the young plant grafting is watered the previous day, and the water content of stock strain reaches more than 75% when making grafting, and scion strain water content is 65%～75%, operating room's indoor temperature is between 25～30 ℃, and young plant does not have water droplet during grafting.
B) grafting: grafting method is a cleft graft, and step is as follows:
(1) the stock strain is fallen its head truncation at the 2nd true leaf place, keeps the bottom, with sterile blade the stem of stock strain is split vertically downward by the center of circle then and is cut 1～1.5cm.
(2) at the thick or thickness similar positions such as cutting part of scion strain, the scion strain is cut off, kept top, with blade its stem is whittled into 1～1.5cm chock then to stock.
(3) scion is inserted closely the position of riving of stock strain, folder is gone up plastic clip then.
4) management
It is 25～28 ℃ that seedling after the grafting is placed on temperature, and humidity is in the sealing plastic canopy more than 85%, to cultivate and after 7～10 days film is opened gradually, and original position is ventilated and fallen the humidity hardening, and hardening can be carried out normal management after 3～5 days, can transplant after 15 days.
Datura (Datura Stramonium L.) and Bu Lita grafting 1600 strains altogether survive 1485 strains, and survival rate reaches 92.8%.
On January 5th, 2008 transplanted datura (Datura Stramonium L.) and Bu Lita grafting in the serious greenhouse of root-knot nematode, and survival rate is 98.8%.
2, productivity of anti-root-knot nematode eggplant grafting and field performance
The selected greenhouse by solar heat root knot nematode disease of field trial is very serious, and it is cucumber that preceding stubble is planted plant, and root-knot nematode harm is very serious, and the underproduction is more than 50%.
2 processing are established in test, are respectively datura (Datura Stramonium L.) and Bu Lita grafting and Bu Lita own-rooted tree (in contrast).Repeat for 4 times, adopt district's group arrangement at random, sub-district area 50m
2, every sub-district field planting 120 strains.In identical environment and condition of culture, be cultured to fruit maturation.
3, the result shows
Since on March 10th, 2008, the Bu Lita own-rooted tree began to have the generation of the sick worm of a small amount of root knot line, and datura (Datura Stramonium L.) is not investigated root knot with the Bu Lita grafting; To on April 25th, 2008, Bu Lita just reached 68.8% from the root knot index of root, and the root knot index of datura (DaturaStramonium L.) and Bu Lita grafting is 2.51%; To on June 10th, 2008, the root knot index of Bu Lita own-rooted tree reached highest level 100%, and the coefficient of datura (Datura StramoniumL.) and Bu Lita grafting only is 3.11%, has only the root knot of minute quantity.Its output is respectively: datura (Datura Stramonium L.) is 2.86 * 10 with the Bu Lita grafting
5Kghm
-2, the Bu Lita own-rooted tree is 1.85 * 10
5Kghm
-2, use datura (Datura Stramonium L.) can increase output 1.01 * 10 than Bu Lita own-rooted tree with the Bu Lita grafting
5Kghm
-2, amount of increase in production reaches 54.6%.
The root knot index calculation method of present embodiment is with embodiment one.
Because of the datura plant poisonous, after people's doubt grafting the eggplant fruit of tying poisonous, through feeding rabbit test, took food continuously 10 days, do not find poisoning symptom, with after moral standing taste, the fruit free from extraneous odour, mouthfeel can relievedly eat with own-rooted tree plant fruit.
Claims (4)
1, a kind of method of cultivating root-knot nematode resistant eggplant, it is characterized in that: this method is as stock with datura, the conventional cultivation eggplant kind of gaogangen nematode is as scion, carries out grafting and obtains grafting and be anti-root-knot nematode eggplant, realizes through the following steps:
1) cultivates
A) with the datura seed in humidity 90%～95%, under 20～28 ℃ of conditions of temperature, stock presprouting of seeds 4～6 days, sowing and the stock strain is cultured to 5～7 true leaves then;
B) planting seed of datura described in the step a) is after 5～7 days, and the eggplant seed that will have gaogangen nematode is in humidity 90%～95%, under 20～28 ℃ of conditions of temperature, and stock presprouting of seeds 2～4 days, sowing and the scion strain is cultured to 4～6 true leaves then;
2) grafting
The stock strain that has 5～7 true leaves described in the step 1) is kept 2 true leaves, with step 2) described in have 4～6 true leaves the scion strain keep 2～3 true leaves and carry out grafting;
3) management
It is 25～28 ℃ that seedling after the grafting is placed on temperature, and humidity is to cultivate 7～10 days in the environment more than 85%;
4) hardening
After 7～10 days, original position is ventilated and is fallen wet hardening with the cultivation of the seedling after the grafting, and hardening can be carried out normal management after 3～5 days, continues to cultivate the eggplant grafting that obtains anti-root-knot nematode.
2, a kind of according to claim 1 method of cultivating root-knot nematode resistant eggplant is characterized in that: step 2) described in grafting method be cleft graft.
3, a kind of according to claim 1 method of cultivating root-knot nematode resistant eggplant is characterized in that: the conventional cultivation eggplant kind of described gaogangen nematode is the long eggplant of peasant.
4, a kind of according to claim 1 method of cultivating root-knot nematode resistant eggplant is characterized in that: the conventional cultivation eggplant kind of described gaogangen nematode is Bu Lita.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008101600958A CN101406131B (en) | 2008-11-17 | 2008-11-17 | Method for cultivating root-knot nematode resistant eggplant |
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| CN2008101600958A CN101406131B (en) | 2008-11-17 | 2008-11-17 | Method for cultivating root-knot nematode resistant eggplant |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612937A (en) * | 2012-01-06 | 2012-08-01 | 谢勇 | Method for controlling Angelica sinensis root-knot nematode by marigold interplanting |
| CN105993493A (en) * | 2016-05-29 | 2016-10-12 | 寿光市新世纪种苗有限公司 | Biological trapping method for controlling colored bell pepper root-knot nematode |
| CN108094000A (en) * | 2017-11-24 | 2018-06-01 | 寿光市新世纪种苗有限公司 | A kind of method for preventing eggplant Bemisia tabaci |
-
2008
- 2008-11-17 CN CN2008101600958A patent/CN101406131B/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612937A (en) * | 2012-01-06 | 2012-08-01 | 谢勇 | Method for controlling Angelica sinensis root-knot nematode by marigold interplanting |
| CN105993493A (en) * | 2016-05-29 | 2016-10-12 | 寿光市新世纪种苗有限公司 | Biological trapping method for controlling colored bell pepper root-knot nematode |
| CN108094000A (en) * | 2017-11-24 | 2018-06-01 | 寿光市新世纪种苗有限公司 | A kind of method for preventing eggplant Bemisia tabaci |
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| CN101406131B (en) | 2010-12-15 |
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