CN101371649B - Method for cultivating weak light-tolerant variety of tomato Ji Dong 216 in protective ground - Google Patents
Method for cultivating weak light-tolerant variety of tomato Ji Dong 216 in protective ground Download PDFInfo
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- CN101371649B CN101371649B CN 200810115941 CN200810115941A CN101371649B CN 101371649 B CN101371649 B CN 101371649B CN 200810115941 CN200810115941 CN 200810115941 CN 200810115941 A CN200810115941 A CN 200810115941A CN 101371649 B CN101371649 B CN 101371649B
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Abstract
The invention discloses a cultivation method of Jidong 216, weak light-resistant breed of tomato in protected areas. Firstly, highly weak light-resistant, high-quality, early maturing and prolific breed is selected as male parent or female parent. Then the female parent and male parent are hybridized. Afterwards, highly weak light-resistant breed with good characteristics of the male parent or female parent is selected from hybridized breeds for cultivation. The invention includes breeding technique of inbred line of weak light-resistant tomato, cultivation method of Jidong 216, a tomato breed in protected areas and method of seed preparation of the tomato breed. Weak light-resistant inbred line obtained by identification and screening is selected as one of the parent material for cultivating hybrid, and combining ability of hybridized combination and weak light-resistance are judged to obtain new breed. The cultivated weak light-resistant breed of tomato in protected areas, Jidong 216 has the advantages of low production cost, high product yield and good quality of fruit.
Description
Technical field
The present invention relates to a kind of breeding technique of variety of crops, relate in particular to a kind of breeding method of protecting ground tomato low light tolerance kind.
Background technology
Tomato is commonly called as tomato, is extensively plantation and is subjected to one of vegetables that consumers in general like deeply.Along with the development of agricultural economy, the production of protection ground tomato more and more is subject to people's attention, and supply plays an important role to s.
In protection ground tomato production process, the low temperature and poor light condition usually causes vine growth and development slow, even stops growing, and disease is serious, has a strong impact on yield and quality.
In the prior art, use fruit-setting agent to wait usually and improve output, and by applying opposing such as agricultural chemicals disease.
Production cost height, product yield are low, and fruit quality is poor.
Summary of the invention
The purpose of this invention is to provide that a kind of production cost is low, product yield is high, and the breeding method in the good east, tomato low light tolerance kind Ji 216, protection ground of fruit quality.
The objective of the invention is to be achieved through the following technical solutions:
The breeding method in east, tomato low light tolerance kind Ji 216, protection of the present invention ground comprises step:
At first, carry out tomato variety parent's selection, specifically select weak light resistance strong or have need proterties a plurality of inbred lines as one of parent material, the general combining ability of the relative fruit-setting rate of the parent material that described weak light resistance is strong is more than 0.1;
Then, described a plurality of inbred lines are joined method by half wheel of diallel cross hybridize, obtain filial generation;
Afterwards, select weak light resistance specific combining ability strong, fruit-setting rate relatively, and have the filial generation that needs proterties that described parent material has and cultivate more than 0.01.
As seen from the above technical solution provided by the invention, the breeding method in east, tomato low light tolerance kind Ji 216, protection of the present invention ground, since at first select weak light resistance strong or have need proterties, the general combining ability of fruit-setting rate is joined method in a plurality of inbred lines 0.1 or more by half wheel of diallel cross and is hybridized the acquisition filial generation relatively; Select weak light resistance specific combining ability strong, fruit-setting rate relatively more than 0.01 afterwards, and have the filial generation that needs proterties that parent material has and cultivate.East, tomato low light tolerance kind Ji, protection ground 216 production costs of cultivating are low, product yield is high, and fruit quality is good.
Embodiment
The breeding method in east, tomato low light tolerance kind Ji 216, protection of the present invention ground, its preferable embodiment is to comprise step:
Concrete select weak light resistance strong or have need proterties a plurality of inbred lines as one of parent material, the general combining ability of the relative fruit-setting rate of the parent material that described weak light resistance is strong is more than 0.1;
Then, described a plurality of inbred lines are joined method by half wheel of diallel cross hybridize, obtain filial generation;
Afterwards, select weak light resistance specific combining ability strong, fruit-setting rate relatively, and have the filial generation that needs proterties that described parent material has and cultivate more than 0.01.
At first, carry out tomato variety parent's selection, specifically select the strong a plurality of inbred lines of weak light resistance as one of parent material, the general combining ability of the relative fruit-setting rate of parent material is more than 0.1.A plurality of inbred lines of selecting can also have other good proterties that needs, as disease-resistant, precocious, high yield etc.;
Then, a plurality of inbred lines of selecting are joined method by half wheel of diallel cross hybridize, obtain filial generation;
Afterwards, from filial generation, select weak light resistance specific combining ability strong, fruit-setting rate relatively, and have the filial generation that needs proterties that parent material has and cultivate more than 0.01.
Carrying out when parent material selects, some good proterties not necessarily are present on one's body male parent or the female parent simultaneously, but might be present in simultaneously among a certain offspring after the hybridization, and can select as required to have needs the Hybrid of proterties to cultivate.
When selecting the strong inbred line of weak light resistance as parent material, need carry out weak light resistance to parental inbred line and identify, specifically comprise step:
At first, select the tangible a plurality of inbred lines of known weak light resistance difference, each inbred line is divided into two groups, and repeat 1 to 3 time, wherein one group is under the normal illumination condition, and another group is carried out shading treatment with the sunshade net, cultivates respectively to the end of gathering;
Then, two groups of plant of each inbred line are investigated in the following proterties one or more respectively: single plant yield, single fruit weight, fruit-setting rate, stem are thick, specific leaf area and dry weight on the ground, and calculate the relative value of the proterties of each inbred line by following formula:
Relative value=(numerical value of the numerical value ÷ normal illumination of shading treatment) * 100%;
Afterwards, according to the result of investigation, a plurality of inbred lines are carried out low light tolerance identify that selecting the strong selfing of weak light resistance is male parent or female parent.
Specifically a plurality of inbred lines being carried out the method that low light tolerance identifies is:
As the low light tolerance direct indicator, through correlation analysis, carry out the low light tolerance regression analysis simultaneously with the whole relative single plant yield of strain, the low light tolerance regression equation of employing is one or more in the following equation:
y＝-0.0604+0.7688x1；
y＝-0.8298+0.7981x1+0.9193x2；
y＝0.8381+0.8555x1+1.0145x2+0.2824x3；
In the formula: y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3, relatively stem is thick.
In the process that maternal and male parent are hybridized, male parent is 1: 5～12 with the field planting ratio of female parent, can adopt 1: 8～10 planting proportion, or other planting proportion etc.
During plantation, male parent was bloomed in advance in 5-7 days than maternal.Male parent and female parent are sowed simultaneously,, male parent is adopted the different sowing time with maternal perhaps according to the difference of kind.
When from filial generation, selecting the strong kind of weak light resistance, at first, described filial generation is carried out weak light resistance identifies that the method for identifying is:
At first, the kind after the hybridization is divided into two groups, and repeats 3 times, wherein one group is under the normal illumination condition, and another group is carried out shading treatment with the sunshade net, cultivates respectively to the end of gathering;
Then, two groups of plant of each kind are investigated following at least a proterties respectively: single plant yield, single fruit weight, fruit-setting rate, root/shoot ratio, plant height, stem are thick, and calculate the relative value of the proterties of each kind by following formula:
Relative value=(numerical value of the numerical value ÷ normal illumination of shading treatment) * 100%;
Afterwards, according to the result of investigation, a plurality of kinds are carried out low light tolerance identify, selecting the strong kind of weak light resistance is east, tomato low light tolerance kind Ji 216, protection of the present invention ground.
Specifically a plurality of kinds of filial generation being carried out the method that low light tolerance identifies is:
As the low light tolerance direct indicator, through correlation analysis, carry out the low light tolerance regression analysis simultaneously with the whole relative single plant yield of strain, the low light tolerance regression equation of employing is one or more in the following equation:
Y＝0.0698+0.3238X1；
Y＝-0.3237+0.4511X2+0.5418X1；
Y＝-0.4190+0.4912X2+0.5724X1+0.0972X3；
In the formula: Y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3, relative root/shoot ratio.
More than selecting strong male parent or the female parent of weak light resistance, or when selecting the strong Hybrid of weak light resistance, the average intensity of illumination under the normal illumination condition can be 4.5～5.5 ten thousand LX; The average intensity of illumination of carrying out after the shading treatment with the sunshade net can be 1.0～1.5 ten thousand LX.
East, tomato low light tolerance kind Ji 216, protection of the present invention ground has following proterties:
Contain vitamin C 17～18mg, soluble solid in every 100g fruit and be 5.5～6.0%, organic acid is 1.0～1.1%); Anti-following one or more diseases: high resisting tobacco mosaic virus disease, high resistance to bacterial wilt, high anti-leaf mold, anti-gray mold; Precocious, high yield.
As required, can make the east, tomato low light tolerance kind Ji 216, protection ground of selection have above one or more proterties.
Specific embodiment:
Utilizing warp is for No. 21 male parent for the stronger high-quality of the weak light resistance of evaluation and screening, disease-resistant, precocious inbred line too much, with the anti-leaf mold of height, late-maturing, the high yield No. 16 be female parent, in the production of hybrid seeds process, male parent and maternal field planting ratio are 1: 8～10, father and mother originally sow simultaneously, and male parent can shift to an earlier date 5-7 days and bloom.Select east, apolegamy combination Ji 216 fruit type seeds of hybridized tomato through artificial hybridization.
Specifically also can handle, identify the weak light resistance of each breeding material, thereby other material that filters out weak light resistance strong disease-resistant (high resisting tobacco mosaic virus disease, bacterial wilt) be male parent or maternal combo by the long-term low light level.
Then coordinate force, weak light resistance, disease resistance, quality, the prematurity of hybridizing the back combination are identified, filter out high-quality and (contain vitamin C 17.68mg in the 100g fruit, soluble solid is 5.83%, and organic acid is 1.029%), the new combination of anti-multiple diseases (high resisting tobacco mosaic virus disease, high resistance to bacterial wilt, high anti-leaf mold, anti-gray mold), precocity, high yield.
Breeding material (male parent or female parent) is carried out the concrete grammar that weak light resistance identifies is:
Select the tangible a plurality of inbred lines of known weak light resistance difference, each selfing ties up to two groups of interior field planting time-divisions of facility, two groups of all district's group arrangements at random, repeat for three times, each sub-district field planting 20～40 strain, one group is in (average intensity of illumination is 50,000 LX) under the normal illumination condition, in contrast, another group is carried out shading treatment (average intensity of illumination is 1.2 ten thousand LX) with black sunshade net, shading is to the end of gathering, decide strain investigation output in the fruit harvesting phase, single fruit weight, fruit-setting rate, stem is thick, specific leaf area and proterties such as dry weight are on the ground calculated the output of each inbred line according to survey data, single fruit weight, fruit-setting rate, stem is thick, the relative value of proterties such as specific leaf area and ground dry weight.The computing formula of relative value: relative value=(numerical value of the numerical value ÷ normal illumination of shading treatment) * 100%.
The instantiation that weak light resistance is identified is carried out in hybrid combination: select 4 parts of inbred lines that weak light resistance is different, carrying out half according to the diallel cross experimental scheme takes turns to join and obtains combination, to be combined in field planting in the greenhouse by solar heat, by district's group arrangement at random, repeat for 3 times, each sub-district field planting 30 strain, line-spacing is 60cm, spacing in the rows is 40cm.One group in the greenhouse normal illumination intensity be made as contrast (intensity of illumination is about 50,000 LX), another group is carried out shading treatment (average intensity of illumination is 1.2 ten thousand LX) with black sunshade net, shading is to the end of gathering, and it is thick to decide strain investigation single plant yield, single fruit weight, fruit-setting rate, root/shoot ratio, plant height, stem in the fruit harvesting phase.
Specifically to the combining ability test of inbred line weak light resistance: joining method by half wheel of diallel cross and obtain inbred line and combination, serves as the main characteristic index of analyzing weak light resistance with relative fruit-setting rate, and the coordinate force of inbred line weak light resistance is analyzed.Under fixed model, the general combining ability of the relative fruit-setting rate of low light tolerance inbred line is more than 0.1, and the specific combining ability of the relative fruit-setting rate of its combination is more than 0.01, and such inbred line and combination weak light resistance ability thereof are strong.
Authentication method to the low light tolerance inbred line is: with relative fruit-setting rate and the extremely significantly positive correlation of relative single plant yield, correlation coefficient is 0.68, relatively single fruit weight, stem is thick and the remarkable positive correlation of relative single plant yield, correlation coefficient is respectively 0.63 and 0.57, carry out the low light tolerance regression analysis simultaneously, the low light tolerance regression equation is:
y＝-0.0604+0.7688x1；
y＝-0.8298+0.7981x1+0.9193x2；
y＝-0.8381+0.8555x1+1.0145x2+0.2824x3；
In the formula: y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3, relatively stem is thick.
Concrete authentication method to the low light tolerance crossbreed is: as the low light tolerance direct indicator, through correlation analysis, fruit-setting rate is extremely significantly positive correlation with relative single plant yield relatively with the whole relative single plant yield of strain, and correlation coefficient is 0.9688; Single fruit weight, root/shoot ratio, plant height, the relative value that stem is thick are negative correlation with relative single plant yield, plant height is remarkable negative correlation with relative single plant yield relatively, correlation coefficient is-0.9172, carries out the regression analysis of low light tolerance combination simultaneously, and low light tolerance combination regression equation is:
Y＝0.0698+0.3238X1；
Y＝-0.3237+0.4511X2+0.5418X1；
Y＝-0.4190+0.4912X2+0.5724X1+0.0972X3；
In the formula: Y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3: relative root/shoot ratio.
The breeding technique and Analysis of Combining Ability, the authentication method of low light tolerance combination, the cultivation in east, special-purpose tomato variety Ji 216, protection ground and the producing method for seed of this tomato variety that comprise low light tolerance tomato inbred line among the present invention.The inbred line of selecting to obtain through evaluation and screening with weak light resistance is identified coordinate force, the weak light resistance of hybrid combination as one of parent material of cultivating crossbreed, thereby is obtained new varieties.East, tomato low light tolerance kind Ji, protection ground 216 production costs of cultivating are low, product yield is high, and fruit quality is good.
The above; only for the preferable embodiment of the present invention, but protection scope of the present invention is not limited thereto, and anyly is familiar with those skilled in the art in the technical scope that the present invention discloses; the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.
Claims (7)
1. a breeding method of protecting east, tomato low light tolerance kind Ji 216, ground is characterized in that, comprises step:
(1) carry out tomato variety parent's selection, specifically select the strong a plurality of inbred lines of weak light resistance as one of parent material, a plurality of inbred lines that described selection weak light resistance is strong comprise step:
At first, select the tangible a plurality of inbred lines of known weak light resistance difference, each inbred line is divided into two groups, and repeats 3 times, wherein one group is under the normal illumination condition, and another group is carried out shading treatment with the sunshade net, cultivates respectively to the end of gathering;
Then, two groups of plant to each inbred line are investigated following at least a proterties respectively in the later stage of gathering: single plant yield, single fruit weight, fruit-setting rate, stem are thick, specific leaf area, dry weight on the ground, and calculate the relative value of the described proterties of each inbred line by following formula:
Relative value=(numerical value of the numerical value ÷ normal illumination of shading treatment) * 100%;
Afterwards, according to the result of investigation, described a plurality of inbred lines are carried out low light tolerance identify that selecting the strong selfing of weak light resistance is one of described parent material, the general combining ability of the relative fruit-setting rate of the parent material that described weak light resistance is strong is more than 0.1;
(2) described a plurality of inbred lines are joined method by half wheel of diallel cross and hybridize, obtain filial generation;
(3) from described filial generation, select the strong kind of weak light resistance:
At first, select the tangible a plurality of kinds of proterties difference from described filial generation, each kind is divided into two groups, and repeats 3 times, and wherein one group is under the normal illumination condition, and another group is carried out shading treatment with the sunshade net, cultivates respectively to the end of gathering;
Then, two groups of plant of each kind are investigated following at least a proterties respectively: single plant yield, single fruit weight, fruit-setting rate, root/shoot ratio, plant height, stem are thick, and calculate the relative value of the described proterties of each kind by following formula:
Relative value=(numerical value of the numerical value ÷ normal illumination of shading treatment) * 100%;
Afterwards, according to the result of investigation, described a plurality of kinds are carried out low light tolerance identify, the specific combining ability of selecting relative fruit-setting rate is more than 0.01, and has the filial generation that needs proterties that described parent material has and cultivate.
2. the breeding method in east, tomato low light tolerance kind Ji 216, protection ground according to claim 1 is characterized in that, described a plurality of inbred lines is carried out the method that low light tolerance identifies be:
To put in order the relative single plant yield of strain as the low light tolerance direct indicator, through correlation analysis, single plant yield and relative single fruit weight, the thick remarkable positive correlation of stem relatively relatively, with the extremely significantly positive correlation of relative fruit-setting rate, carry out the low light tolerance regression analysis simultaneously, the low light tolerance regression equation of employing is one or more in the following equation:
y＝-0.0604+0.7688x1；
y＝-0.8298+0.7981x1+0.9193x2；
y＝-0.8381+0.8555x1+1.0145x2+0.2824x3；
In the formula: y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3, relatively stem is thick.
3. the breeding method in east, tomato low light tolerance kind Ji 216, protection according to claim 2 ground is characterized in that in the process that female parent and male parent are hybridized, male parent and female parent are sowed simultaneously.
4. the breeding method in east, tomato low light tolerance kind Ji 216, protection ground according to claim 1 is characterized in that, in the process that described a plurality of inbred lines are hybridized, male parent was bloomed in advance than maternal in 5-7 days.
5. the breeding method in east, tomato low light tolerance kind Ji 216, protection ground according to claim 1 is characterized in that, a plurality of kinds of described filial generation is carried out the method that low light tolerance identifies be:
To put in order the relative single plant yield of strain as the low light tolerance direct indicator, through correlation analysis, the extremely remarkable positive correlation of single plant yield and relative fruit-setting rate relatively, with relative single fruit weight, relative root/shoot ratio negative correlation, carry out the low light tolerance regression analysis simultaneously, the low light tolerance regression equation of employing is one or more in the following equation:
Y＝0.0698+0.3238X1；
Y＝-0.3237+0.4511X2+0.5418X1；
Y＝-0.4190+0.4912X2+0.5724X1+0.0972X3；
In the formula: Y, relative single plant yield; X1, relative fruit-setting rate; X2, relative single fruit weight; X3, relative root/shoot ratio.
6. the breeding method in east, tomato low light tolerance kind Ji 216, protection according to claim 1 ground is characterized in that the average intensity of illumination under the described normal illumination condition is 4.5～5.5 ten thousand LX; Described average intensity of illumination of carrying out after the shading treatment with the sunshade net is 1.0～1.5 ten thousand LX.
7. the breeding method in east, tomato low light tolerance kind Ji 216, protection according to claim 1 ground is characterized in that, east, tomato low light tolerance kind Ji 216, described protection ground has following proterties:
Contain vitamin C 17～18mg in every 100g fruit, soluble solid is 5.5～6.5%, and organic acid is 1.0～1.1%;
Anti-following one or more diseases: high resisting tobacco mosaic virus disease, high resistance to bacterial wilt, high anti-leaf mold, anti-gray mold.
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| CN 200810115941 CN101371649B (en) | 2008-06-30 | 2008-06-30 | Method for cultivating weak light-tolerant variety of tomato Ji Dong 216 in protective ground |
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| CN 200810115941 CN101371649B (en) | 2008-06-30 | 2008-06-30 | Method for cultivating weak light-tolerant variety of tomato Ji Dong 216 in protective ground |
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| CN101371649B true CN101371649B (en) | 2011-11-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104541875A (en) * | 2014-12-31 | 2015-04-29 | 安徽徽大农业有限公司 | Yellow cherry tomato scion cutting breeding method |
| CN111149691A (en) * | 2020-03-20 | 2020-05-15 | 山东永盛农业发展有限公司 | Method for breeding weak light-resistant inbred line by utilizing tomato hybrid |
| CN111149692A (en) * | 2020-03-20 | 2020-05-15 | 山东永盛农业发展有限公司 | Method for cultivating early-maturing low-temperature-resistant inbred line of tomato |
| CN113197029A (en) * | 2021-05-25 | 2021-08-03 | 上海市农业科学院 | Cultivation method and device for improving weak light resistance of tomatoes |
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2008
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Non-Patent Citations (3)
| Title |
|---|
| 席海军,等.番茄弱光耐受性研究进展.《北方园艺》.2007,(第2期),39-41. * |
| 毛秀杰,等.保护地栽培专用鲜食小果型番茄新品种‘冀东216’.《园艺学报》.2007,第34卷(第1期),265. * |
| 黄伟，等.番茄耐低温弱光性研究进展.《河北北方植物学报（自然科学版）》.2005,第21卷(第2期),46-48. * |
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