CN101365805A - 使用蛋白质互补在体内实时检测核酸 - Google Patents

使用蛋白质互补在体内实时检测核酸 Download PDF

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Publication number
CN101365805A
CN101365805A CNA2006800498010A CN200680049801A CN101365805A CN 101365805 A CN101365805 A CN 101365805A CN A2006800498010 A CNA2006800498010 A CN A2006800498010A CN 200680049801 A CN200680049801 A CN 200680049801A CN 101365805 A CN101365805 A CN 101365805A
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nucleic acid
protein
acid binding
rna
sub
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Chinese (zh)
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N·布劳德
C·R·坎托
M·伯顿
V·V·德米达夫
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Boston University
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Boston University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/107—Enzyme complementation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNA2006800498010A 2005-10-27 2006-10-27 使用蛋白质互补在体内实时检测核酸 Pending CN101365805A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73074605P 2005-10-27 2005-10-27
US60/730,746 2005-10-27

Publications (1)

Publication Number Publication Date
CN101365805A true CN101365805A (zh) 2009-02-11

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CNA2006800498010A Pending CN101365805A (zh) 2005-10-27 2006-10-27 使用蛋白质互补在体内实时检测核酸

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Country Link
US (1) US20090029370A1 (https=)
EP (1) EP1954828A2 (https=)
JP (1) JP2009513141A (https=)
KR (1) KR20080070837A (https=)
CN (1) CN101365805A (https=)
AU (1) AU2006305901A1 (https=)
CA (1) CA2627410A1 (https=)
IL (1) IL191064A0 (https=)
WO (1) WO2007050979A2 (https=)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868158A (zh) * 2017-03-17 2017-06-20 广东省生态环境技术研究所 一种沙门氏菌的检测方法及检测试剂盒
CN106868157A (zh) * 2017-03-17 2017-06-20 广东省生态环境技术研究所 一种金黄色葡萄球菌的检测方法及检测试剂盒
CN108267595A (zh) * 2017-11-27 2018-07-10 南京天纵易康生物科技股份有限公司 一种基于双分子荧光互补技术的Myo检测试剂盒、制备及使用方法
CN110455756A (zh) * 2019-07-26 2019-11-15 重庆工商大学 一种同时检测二价铅离子和二价铜离子的方法
CN113092461A (zh) * 2021-04-09 2021-07-09 四川大学华西医院 一种通过选择性识别叶酸受体的循环肿瘤细胞的均相二维可视化/荧光分析方法及应用
CN114252622A (zh) * 2020-09-23 2022-03-29 中国科学院大连化学物理研究所 一种体外筛选新型冠状病毒抑制剂的方法
CN115855920A (zh) * 2022-08-29 2023-03-28 江苏科技大学 智能手机APP结合DSN酶微流体芯片比色检测miRNA-21的方法

Families Citing this family (9)

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US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
EP1948825A2 (en) * 2005-10-27 2008-07-30 The Trustees Of Boston University Activated split-polypeptides and methods for their production and use
KR20090073255A (ko) * 2006-10-27 2009-07-02 트르스티스 오브 보스톤 유니버시티 질병, 악성 종양 및 장애를 치료하기 위한 표적화 분열 생체 분자성 접합체, 및 그의 생성 방법
AU2008308457A1 (en) * 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
WO2011135040A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag Fluorescent antibody fusion protein, its production and use
WO2019018423A1 (en) * 2017-07-17 2019-01-24 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGISTS AND ASSOCIATED SYSTEMS
WO2020018509A2 (en) * 2018-07-16 2020-01-23 Massachusetts Institute Of Technology Rna tickertape for recording transcriptional histories of cells
JPWO2021010442A1 (https=) * 2019-07-16 2021-01-21
CN113504274B (zh) * 2021-07-20 2023-11-21 郑州轻工业大学 一种共价有机骨架材料及其制备方法和应用，适配体传感器及其制备方法

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CA2075858C (en) * 1991-08-15 1998-05-19 Scott J. Eisenbeis Detection of complementary nucleotide sequences
JPH08500965A (ja) * 1992-05-22 1996-02-06 デイナ ファーバー キャンサー インスチチュート ハイブリッドウイルス発現ベクター，その使用および新規アッセイ
AU7401394A (en) * 1993-07-20 1995-02-20 University Of Massachusetts Medical Center In vivo nucleic acid hybridization method
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
EP0878552A1 (en) * 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
US7166424B2 (en) * 1998-02-02 2007-01-23 Odyssey Thera Inc. Fragments of fluorescent proteins for protein fragment complementation assays
US6287772B1 (en) * 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
AU766499B2 (en) * 1998-06-23 2003-10-16 Medical Research Council Structured antisense nucleic acid molecules
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
AU781992B2 (en) * 2000-04-04 2005-06-23 Medical Research Council Methods involving induced dimerisation by cellular components
US6951947B2 (en) * 2000-07-13 2005-10-04 The Scripps Research Institute Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation
GB0226727D0 (en) * 2002-11-15 2002-12-24 Medical Res Council Intrabodies
US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
GB0226729D0 (en) * 2002-11-15 2002-12-24 Medical Res Council Intracellular antibodies
EP1877583A2 (en) * 2005-05-05 2008-01-16 Arizona Board of Regents on behalf of the Unversity of Arizona Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences
EP1948825A2 (en) * 2005-10-27 2008-07-30 The Trustees Of Boston University Activated split-polypeptides and methods for their production and use

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868158A (zh) * 2017-03-17 2017-06-20 广东省生态环境技术研究所 一种沙门氏菌的检测方法及检测试剂盒
CN106868157A (zh) * 2017-03-17 2017-06-20 广东省生态环境技术研究所 一种金黄色葡萄球菌的检测方法及检测试剂盒
CN106868158B (zh) * 2017-03-17 2019-08-27 广东省生态环境技术研究所 一种沙门氏菌的检测方法及检测试剂盒
CN106868157B (zh) * 2017-03-17 2019-08-27 广东省生态环境技术研究所 一种金黄色葡萄球菌的检测方法及检测试剂盒
CN108267595A (zh) * 2017-11-27 2018-07-10 南京天纵易康生物科技股份有限公司 一种基于双分子荧光互补技术的Myo检测试剂盒、制备及使用方法
CN110455756A (zh) * 2019-07-26 2019-11-15 重庆工商大学 一种同时检测二价铅离子和二价铜离子的方法
CN110455756B (zh) * 2019-07-26 2022-03-29 重庆工商大学 一种同时检测二价铅离子和二价铜离子的方法
CN114252622A (zh) * 2020-09-23 2022-03-29 中国科学院大连化学物理研究所 一种体外筛选新型冠状病毒抑制剂的方法
CN113092461A (zh) * 2021-04-09 2021-07-09 四川大学华西医院 一种通过选择性识别叶酸受体的循环肿瘤细胞的均相二维可视化/荧光分析方法及应用
CN113092461B (zh) * 2021-04-09 2023-08-22 四川大学华西医院 一种均相二维可视化/荧光分析方法及应用
CN115855920A (zh) * 2022-08-29 2023-03-28 江苏科技大学 智能手机APP结合DSN酶微流体芯片比色检测miRNA-21的方法
CN115855920B (zh) * 2022-08-29 2026-04-14 江苏科技大学 智能手机APP结合DSN酶微流体芯片比色检测miRNA-21的方法

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Publication number Publication date
AU2006305901A1 (en) 2007-05-03
IL191064A0 (en) 2008-12-29
WO2007050979A2 (en) 2007-05-03
US20090029370A1 (en) 2009-01-29
KR20080070837A (ko) 2008-07-31
JP2009513141A (ja) 2009-04-02
EP1954828A2 (en) 2008-08-13
WO2007050979A3 (en) 2007-07-26
CA2627410A1 (en) 2007-05-03

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Application publication date: 20090211