AU2006305901A1 - Real time nucleic acid detection in vivo using protein complementation - Google Patents

Real time nucleic acid detection in vivo using protein complementation Download PDF

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Publication number
AU2006305901A1
AU2006305901A1 AU2006305901A AU2006305901A AU2006305901A1 AU 2006305901 A1 AU2006305901 A1 AU 2006305901A1 AU 2006305901 A AU2006305901 A AU 2006305901A AU 2006305901 A AU2006305901 A AU 2006305901A AU 2006305901 A1 AU2006305901 A1 AU 2006305901A1
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AU
Australia
Prior art keywords
nucleic acid
protein
detector
rna
acid binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2006305901A
Other languages
English (en)
Inventor
Natalia Broude
Maria Burton
Charles R. Cantor
Vadim V. Demidov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Publication of AU2006305901A1 publication Critical patent/AU2006305901A1/en
Assigned to TRUSTEES OF BOSTON UNIVERSITY reassignment TRUSTEES OF BOSTON UNIVERSITY Alteration of Name(s) of Applicant(s) under S113 Assignors: THE TRUSTEES OF BOSTON UNIVERSITY
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/107—Enzyme complementation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2006305901A 2005-10-27 2006-10-27 Real time nucleic acid detection in vivo using protein complementation Abandoned AU2006305901A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US73074605P 2005-10-27 2005-10-27
US60/730,746 2005-10-27
PCT/US2006/042210 WO2007050979A2 (en) 2005-10-27 2006-10-27 Real time nucleic acid detection in vivo using protein complementation

Publications (1)

Publication Number Publication Date
AU2006305901A1 true AU2006305901A1 (en) 2007-05-03

Family

ID=37968606

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006305901A Abandoned AU2006305901A1 (en) 2005-10-27 2006-10-27 Real time nucleic acid detection in vivo using protein complementation

Country Status (9)

Country Link
US (1) US20090029370A1 (https=)
EP (1) EP1954828A2 (https=)
JP (1) JP2009513141A (https=)
KR (1) KR20080070837A (https=)
CN (1) CN101365805A (https=)
AU (1) AU2006305901A1 (https=)
CA (1) CA2627410A1 (https=)
IL (1) IL191064A0 (https=)
WO (1) WO2007050979A2 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
EP1948825A2 (en) * 2005-10-27 2008-07-30 The Trustees Of Boston University Activated split-polypeptides and methods for their production and use
KR20090073255A (ko) * 2006-10-27 2009-07-02 트르스티스 오브 보스톤 유니버시티 질병, 악성 종양 및 장애를 치료하기 위한 표적화 분열 생체 분자성 접합체, 및 그의 생성 방법
AU2008308457A1 (en) * 2007-10-04 2009-04-09 Halcyon Molecular Sequencing nucleic acid polymers with electron microscopy
WO2011135040A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag Fluorescent antibody fusion protein, its production and use
CN106868157B (zh) * 2017-03-17 2019-08-27 广东省生态环境技术研究所 一种金黄色葡萄球菌的检测方法及检测试剂盒
CN106868158B (zh) * 2017-03-17 2019-08-27 广东省生态环境技术研究所 一种沙门氏菌的检测方法及检测试剂盒
WO2019018423A1 (en) * 2017-07-17 2019-01-24 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGISTS AND ASSOCIATED SYSTEMS
CN108267595A (zh) * 2017-11-27 2018-07-10 南京天纵易康生物科技股份有限公司 一种基于双分子荧光互补技术的Myo检测试剂盒、制备及使用方法
WO2020018509A2 (en) * 2018-07-16 2020-01-23 Massachusetts Institute Of Technology Rna tickertape for recording transcriptional histories of cells
JPWO2021010442A1 (https=) * 2019-07-16 2021-01-21
CN110455756B (zh) * 2019-07-26 2022-03-29 重庆工商大学 一种同时检测二价铅离子和二价铜离子的方法
CN114252622A (zh) * 2020-09-23 2022-03-29 中国科学院大连化学物理研究所 一种体外筛选新型冠状病毒抑制剂的方法
CN113092461B (zh) * 2021-04-09 2023-08-22 四川大学华西医院 一种均相二维可视化/荧光分析方法及应用
CN113504274B (zh) * 2021-07-20 2023-11-21 郑州轻工业大学 一种共价有机骨架材料及其制备方法和应用，适配体传感器及其制备方法
CN115855920B (zh) * 2022-08-29 2026-04-14 江苏科技大学 智能手机APP结合DSN酶微流体芯片比色检测miRNA-21的方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2075858C (en) * 1991-08-15 1998-05-19 Scott J. Eisenbeis Detection of complementary nucleotide sequences
JPH08500965A (ja) * 1992-05-22 1996-02-06 デイナ ファーバー キャンサー インスチチュート ハイブリッドウイルス発現ベクター，その使用および新規アッセイ
AU7401394A (en) * 1993-07-20 1995-02-20 University Of Massachusetts Medical Center In vivo nucleic acid hybridization method
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6294330B1 (en) * 1997-01-31 2001-09-25 Odyssey Pharmaceuticals Inc. Protein fragment complementation assays for the detection of biological or drug interactions
EP0878552A1 (en) * 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
US7166424B2 (en) * 1998-02-02 2007-01-23 Odyssey Thera Inc. Fragments of fluorescent proteins for protein fragment complementation assays
US6287772B1 (en) * 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
AU766499B2 (en) * 1998-06-23 2003-10-16 Medical Research Council Structured antisense nucleic acid molecules
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
AU781992B2 (en) * 2000-04-04 2005-06-23 Medical Research Council Methods involving induced dimerisation by cellular components
US6951947B2 (en) * 2000-07-13 2005-10-04 The Scripps Research Institute Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation
GB0226727D0 (en) * 2002-11-15 2002-12-24 Medical Res Council Intrabodies
US7662554B2 (en) * 2002-10-09 2010-02-16 The Trustees Of Boston University Nucleic acid supported protein complementation
GB0226729D0 (en) * 2002-11-15 2002-12-24 Medical Res Council Intracellular antibodies
EP1877583A2 (en) * 2005-05-05 2008-01-16 Arizona Board of Regents on behalf of the Unversity of Arizona Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences
EP1948825A2 (en) * 2005-10-27 2008-07-30 The Trustees Of Boston University Activated split-polypeptides and methods for their production and use

Also Published As

Publication number Publication date
IL191064A0 (en) 2008-12-29
WO2007050979A2 (en) 2007-05-03
CN101365805A (zh) 2009-02-11
US20090029370A1 (en) 2009-01-29
KR20080070837A (ko) 2008-07-31
JP2009513141A (ja) 2009-04-02
EP1954828A2 (en) 2008-08-13
WO2007050979A3 (en) 2007-07-26
CA2627410A1 (en) 2007-05-03

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