CN101300352B - 检测、分析和鉴定基因组dna的方法和分子诊断装置 - Google Patents

检测、分析和鉴定基因组dna的方法和分子诊断装置 Download PDF

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CN101300352B
CN101300352B CN2006800405779A CN200680040577A CN101300352B CN 101300352 B CN101300352 B CN 101300352B CN 2006800405779 A CN2006800405779 A CN 2006800405779A CN 200680040577 A CN200680040577 A CN 200680040577A CN 101300352 B CN101300352 B CN 101300352B
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microfluidic
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CN101300352A (zh
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井上裕司
I·T·奈特
G·A·戴尔
R·R·科维尔
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Canon USA Inc
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2006800405779A 2005-09-01 2006-09-01 检测、分析和鉴定基因组dna的方法和分子诊断装置 Active CN101300352B (zh)

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US71281305P 2005-09-01 2005-09-01
US60/712,813 2005-09-01
PCT/US2006/034336 WO2007028084A2 (en) 2005-09-01 2006-09-01 Method and molecular diagnostic device for detection, analysis and identification of genomic dna

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CN101300352B true CN101300352B (zh) 2013-03-20

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Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US7915030B2 (en) * 2005-09-01 2011-03-29 Canon U.S. Life Sciences, Inc. Method and molecular diagnostic device for detection, analysis and identification of genomic DNA
WO2007081385A2 (en) * 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7598091B2 (en) * 2006-04-04 2009-10-06 Micropoint Bioscience, Inc. Micromachined diagnostic device with controlled flow of fluid and reaction
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US7745129B1 (en) * 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
US8148159B2 (en) * 2006-10-05 2012-04-03 Massachusetts Institute Of Technology System and methods for stretching polynucleotides
US20080167198A1 (en) * 2007-01-04 2008-07-10 Christopher Gerard Cooney Filter based detection system
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP2717041B1 (en) * 2007-06-11 2015-05-27 Wako Pure Chemical Industries, Ltd. Microchip large volume polymerase chain reaction (PCR) with integrated real-time capillary electrophoresis detection
CA2699315A1 (en) * 2007-09-17 2009-03-26 Red Ivory Llc Self-actuating signal producing detection devices and methods
PE20090965A1 (es) * 2007-10-12 2009-07-13 Bigtec Private Ltd Micro chip
US9114397B2 (en) * 2007-10-25 2015-08-25 Canon U.S. Life Sciences, Inc. Method of reducing cross-contamination in continuous amplification reactions in a channel
CA2705213C (en) * 2007-11-07 2016-10-04 The University Of British Columbia Microfluidic device and method of using same
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
RU2380418C1 (ru) * 2008-10-01 2010-01-27 Учреждение Российской академии наук Институт молекулярной биологии им. В.А. Энгельгардта РАН Сменный микрофлюидный модуль для автоматизированного выделения и очистки нуклеиновых кислот из биологических образцов и способ выделения и очистки нуклеиновых кислот с его использованием
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
CN104722342B (zh) 2009-03-24 2017-01-11 芝加哥大学 滑动式芯片装置和方法
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US8354080B2 (en) 2009-04-10 2013-01-15 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
WO2010141131A1 (en) 2009-06-04 2010-12-09 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
EP2439512A1 (en) * 2010-10-01 2012-04-11 Aqsens Oy A device for holding a sample
AU2011315951B2 (en) 2010-10-15 2015-03-19 Lockheed Martin Corporation Micro fluidic optic design
JP2013545475A (ja) * 2010-11-30 2013-12-26 クワンタムディーエックス・グループ・リミテッド マイクロ流体多重温度可撓性反応デバイスの設計、製造及び使用
GB2486435A (en) * 2010-12-14 2012-06-20 Morteza Bahrami Liquid sample receiving apparatus
KR20140063507A (ko) * 2011-02-07 2014-05-27 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 액적을 분할하기 위한 시스템 및 방법
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US11198126B2 (en) * 2011-10-31 2021-12-14 Fluid-Screen, Inc. Apparatus for pathogen detection
WO2013074796A1 (en) * 2011-11-15 2013-05-23 The Board Of Trustees Of The University Of Illinois Thermal control of droplets by nanoscale field effect transistors
US9475013B2 (en) 2012-02-08 2016-10-25 President And Fellows Of Harvard College Droplet formation using fluid breakup
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
WO2013132645A1 (ja) * 2012-03-09 2013-09-12 独立行政法人産業技術総合研究所 核酸増幅方法
DE102012205171B3 (de) * 2012-03-29 2013-09-12 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen
US9551030B2 (en) * 2012-06-17 2017-01-24 Pacific Biosciences Of California, Inc. Filter architecture for analytical devices
US9366647B2 (en) * 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
JP2014176302A (ja) * 2013-03-13 2014-09-25 Seiko Epson Corp 核酸増幅反応装置及び核酸増幅方法
US9718056B2 (en) 2013-03-15 2017-08-01 Syracuse University Microfluidics polymerase chain reaction and high resolution melt detection
WO2014187926A1 (en) * 2013-05-22 2014-11-27 Imec Vzw Compact fluid analysis device and method to fabricate
CN105636697B (zh) * 2013-09-30 2018-06-12 基纽拜奥股份有限公司 微流体盒装置和使用方法以及组件
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US10352899B2 (en) 2014-10-06 2019-07-16 ALVEO Technologies Inc. System and method for detection of silver
US9506908B2 (en) 2014-10-06 2016-11-29 Alveo Technologies, Inc. System for detection of analytes
US10627358B2 (en) 2014-10-06 2020-04-21 Alveo Technologies, Inc. Method for detection of analytes
US9921182B2 (en) 2014-10-06 2018-03-20 ALVEO Technologies Inc. System and method for detection of mercury
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
US20160116409A1 (en) * 2014-10-28 2016-04-28 Omnivision Technologies, Inc. Color-Sensitive Image Sensor With Embedded Microfluidics And Associated Methods
WO2016209735A1 (en) 2015-06-22 2016-12-29 Fluxergy, Llc Camera imaging system for a fluid sample assay and method of using same
WO2016209734A1 (en) 2015-06-22 2016-12-29 Fluxergy, Llc Device for analyzing a fluid sample and use of test card with same
WO2016209731A1 (en) 2015-06-22 2016-12-29 Fluxergy, Llc Test card for assay and method of manufacturing same
CA3224549C (en) * 2015-07-17 2025-09-09 Cue Health Inc. SYSTEMS AND METHODS FOR IMPROVED DETECTION AND QUANTIFICATION OF SUBSTANCES TO BE ANALYZED
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US10882045B2 (en) 2016-01-08 2021-01-05 Hewlett-Packard Development Company, L.P. Polymerase chain reaction device including ejection nozzles
CA3011710A1 (en) * 2016-01-20 2017-07-27 Triv Tech, Llc Point-of-care nucleic acid amplification and detection
JP7099965B2 (ja) * 2016-06-03 2022-07-12 パーデュー・リサーチ・ファウンデーション 吸着材料を使用して試料から抽出された分析物を分析するためのシステムおよび方法
KR20220151011A (ko) 2016-09-23 2022-11-11 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
CN107151700B (zh) * 2017-06-08 2023-11-07 杭州遂真生物技术有限公司 一种基因检测方法及基因检测试剂盒和基因检测设备
CN107527595B (zh) * 2017-09-27 2019-06-07 京东方科技集团股份有限公司 一种微流控系统及其驱动方法
US12071657B2 (en) 2017-10-20 2024-08-27 Hewlett-Packard Development Company, L.P. Nucleic acid amplification
US20210031188A1 (en) 2018-04-24 2021-02-04 Hewlett-Packard Development Company, L.P. Droplet ejectors to draw fluids through microfluidic networks
US11925932B2 (en) 2018-04-24 2024-03-12 Hewlett-Packard Development Company, L.P. Microfluidic devices
US11325380B2 (en) 2018-07-17 2022-05-10 Hewlett-Packard Development Company, L.P. Droplet ejectors to provide fluids to droplet ejectors
US11547993B2 (en) 2018-07-17 2023-01-10 Hewlett-Packard Development Company, L.P. Droplet ejectors with target media
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
MX2021007297A (es) 2018-12-20 2021-09-08 Alveo Tech Inc Sistema portatil de prueba de diagnostico basado en impedancias para detectar analitos.
EP3902632A1 (en) * 2018-12-28 2021-11-03 Astraveus Device and method for handling a particle suspension
US11195864B2 (en) 2019-03-01 2021-12-07 Omnivision Technologies, Inc. Flip-chip sample imaging devices with self-aligning lid
WO2020185200A1 (en) * 2019-03-08 2020-09-17 Hewlett-Packard Development Company, L.P. Disposable microfluidic cassettes
CN114641450A (zh) * 2019-09-09 2022-06-17 路玛赛特有限责任公司 使用光力和拉曼光谱取样和分析细胞的微流体装置和方法
US20210205813A1 (en) * 2019-12-23 2021-07-08 National Technology & Engineering Solutions Of Sandia, Llc Contactless liquid loading to microfluidic devices
WO2022010461A1 (en) * 2020-07-07 2022-01-13 Hewlett-Packard Development Company, L.P. Microfluidic sensing
US12472492B2 (en) 2020-08-14 2025-11-18 Alveo Technologies, Inc. Systems and methods of sample depositing and testing
KR102416335B1 (ko) * 2021-06-29 2022-07-05 에스디바이오센서 주식회사 기체 이동 통로와 추출액 이동 통로를 갖는 증폭 모듈
CN113552379A (zh) * 2021-08-24 2021-10-26 中国科学院上海微系统与信息技术研究所 一种多通道液滴采样装置、包括其的光学分析系统以及方法
US12266579B2 (en) * 2021-08-30 2025-04-01 Taiwan Semiconductor Manufacturing Co., Ltd. Method and system for adjusting the gap between a wafer and a top plate in a thin-film deposition process
DE102023129147A1 (de) 2023-10-24 2025-04-24 Technische Universität Dresden, Körperschaft des öffentlichen Rechts Fluidische Vorrichtung und Verfahren zur Probenvorbereitung mit einer fluidischen Vorrichtung

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1127227A (en) * 1977-10-03 1982-07-06 Ichiro Endo Liquid jet recording process and apparatus therefor
US4330787A (en) 1978-10-31 1982-05-18 Canon Kabushiki Kaisha Liquid jet recording device
US4345262A (en) 1979-02-19 1982-08-17 Canon Kabushiki Kaisha Ink jet recording method
US4463359A (en) * 1979-04-02 1984-07-31 Canon Kabushiki Kaisha Droplet generating method and apparatus thereof
US4313124A (en) * 1979-05-18 1982-01-26 Canon Kabushiki Kaisha Liquid jet recording process and liquid jet recording head
US4558333A (en) * 1981-07-09 1985-12-10 Canon Kabushiki Kaisha Liquid jet recording head
JPS59123670A (ja) 1982-12-28 1984-07-17 Canon Inc インクジエツトヘツド
JPS59138461A (ja) 1983-01-28 1984-08-08 Canon Inc 液体噴射記録装置
US5714380A (en) * 1986-10-23 1998-02-03 Amoco Corporation Closed vessel for isolating target molecules and for performing amplification
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
GB9608644D0 (en) * 1996-04-26 1996-07-03 Scient Genarics Ltd Nucleic acid manipulation
US6267858B1 (en) * 1996-06-28 2001-07-31 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
US6221654B1 (en) * 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
JP4274561B2 (ja) * 1997-08-01 2009-06-10 キヤノン株式会社 プローブアレイとその製造方法
JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
US5965410A (en) 1997-09-02 1999-10-12 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
DE69839133T2 (de) 1997-12-06 2009-02-05 Invitrogen Corp., Carlsbad Isolierung von Nukleinsäuren
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6500323B1 (en) 1999-03-26 2002-12-31 Caliper Technologies Corp. Methods and software for designing microfluidic devices
JP2003524183A (ja) * 2000-02-23 2003-08-12 カリパー・テクノロジーズ・コープ． マルチリザーバ圧力コントロールシステム
WO2002010373A2 (en) * 2000-07-31 2002-02-07 Applera Corporation Apparatus and method for specific release of captured extension products
EP1309863A1 (en) * 2000-08-08 2003-05-14 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
JP4949600B2 (ja) 2000-09-14 2012-06-13 カリパー・ライフ・サイエンシズ・インク． 温度介在反応を行うためのマイクロ流体装置及び方法
AU2002241515A1 (en) * 2000-11-06 2002-06-18 The University Of Houston System Nucleic acid separation using immobilized metal affinity chromatography
EP1355823A4 (en) * 2001-01-29 2005-04-20 Caliper Life Sciences Inc NON-MECHANICAL VALVES FOR FLUID SYSTEMS
WO2002061392A2 (en) 2001-01-31 2002-08-08 Board Of Regents, The University Of Texas System Method and apparatus for the confinement of materials in a micromachined chemical sensor array
JP2003014773A (ja) * 2001-03-28 2003-01-15 Canon Inc プローブ担体、その製造装置およびその製造方法
DK1439897T3 (da) * 2001-10-11 2011-01-03 Aviva Biosciences Corp Fremgangsmåder til adskillelse af sjældne celler fra væskeprøver
US20030138819A1 (en) * 2001-10-26 2003-07-24 Haiqing Gong Method for detecting disease
JP2003180351A (ja) 2001-12-13 2003-07-02 Adgene Co Ltd 核酸高次構造誘導を用いた核酸変異検出法
JP2003334082A (ja) 2002-05-17 2003-11-25 Adgene Co Ltd 波形生成プライマー、該プライマーを使用した核酸増幅方法及び核酸同定方法
JP2004037112A (ja) * 2002-06-28 2004-02-05 Canon Inc 核酸チップ基板、及び核酸チップの検査方法、並びに製造方法
JP3937954B2 (ja) * 2002-07-18 2007-06-27 キヤノン株式会社 プローブ担体の製造方法および製造装置
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US20060210984A1 (en) * 2003-03-03 2006-09-21 Jeremy Lambert Use of nucleic acid mimics for internal reference and calibration in a flow cell microarray binding assay
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
JP2005024278A (ja) * 2003-06-30 2005-01-27 Canon Inc プローブ担体の製造方法及び製造装置
JP2006198523A (ja) * 2005-01-20 2006-08-03 Fuji Photo Film Co Ltd 加圧エア供給機構、加圧装置、及び抽出機
EP1871903B1 (en) * 2005-02-18 2011-12-21 Canon U.S. Life Sciences, Inc. Devices and methods for identifying genomic dna of organisms
US7915030B2 (en) * 2005-09-01 2011-03-29 Canon U.S. Life Sciences, Inc. Method and molecular diagnostic device for detection, analysis and identification of genomic DNA

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杨军等.芯片实验室( 微流芯片) 技术.《生物化学与生物物理学报》.2002, *

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