CN101255476A - 用于制备检测dna甲基化试样的试样处理液和试剂盒 - Google Patents

用于制备检测dna甲基化试样的试样处理液和试剂盒 Download PDF

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Publication number
CN101255476A
CN101255476A CNA2008100083371A CN200810008337A CN101255476A CN 101255476 A CN101255476 A CN 101255476A CN A2008100083371 A CNA2008100083371 A CN A2008100083371A CN 200810008337 A CN200810008337 A CN 200810008337A CN 101255476 A CN101255476 A CN 101255476A
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Prior art keywords
dna
treatment solution
sample treatment
sample
dna methylation
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Pending
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CNA2008100083371A
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English (en)
Chinese (zh)
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梶田昌裕
酒井绫子
山本宪明
石原英干
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Sysmex Corp
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Sysmex Corp
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Publication of CN101255476A publication Critical patent/CN101255476A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNA2008100083371A 2007-02-28 2008-02-26 用于制备检测dna甲基化试样的试样处理液和试剂盒 Pending CN101255476A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007050748 2007-02-28
JP2007050748A JP2008212009A (ja) 2007-02-28 2007-02-28 Dnaメチル化検出用試料の調製方法

Publications (1)

Publication Number Publication Date
CN101255476A true CN101255476A (zh) 2008-09-03

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CNA2008100083371A Pending CN101255476A (zh) 2007-02-28 2008-02-26 用于制备检测dna甲基化试样的试样处理液和试剂盒

Country Status (4)

Country Link
US (1) US20080206772A1 (enExample)
EP (1) EP1964934A1 (enExample)
JP (1) JP2008212009A (enExample)
CN (1) CN101255476A (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法
CA2708163A1 (en) * 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
US10527526B2 (en) 2011-11-03 2020-01-07 Tripath Imaging, Inc. Methods and compositions for preparing samples for immunostaining
CN111235227A (zh) * 2020-03-31 2020-06-05 西安天隆科技有限公司 一种游离dna提取及甲基化转化方法、试剂及试剂盒
CN116323977A (zh) * 2020-07-30 2023-06-23 剑桥表现遗传学有限公司 用于核酸分析的组合物和方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
DE69937223T3 (de) 1998-11-09 2011-05-05 Eiken Kagaku K.K. Verfahren zur synthese von nukleinsäuren
US7718364B2 (en) * 2003-03-25 2010-05-18 John Wayne Cancer Institute DNA markers for management of cancer
JP4166725B2 (ja) 2003-07-31 2008-10-15 独立行政法人科学技術振興機構 メチル化dna検出方法
JP4435174B2 (ja) * 2003-12-02 2010-03-17 エフ.ホフマン−ラ ロシュ アーゲー 重亜硫酸塩処理の改良された方法
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (en) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method

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Publication number Publication date
JP2008212009A (ja) 2008-09-18
US20080206772A1 (en) 2008-08-28
EP1964934A1 (en) 2008-09-03

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Open date: 20080903