JP2008212009A - Dnaメチル化検出用試料の調製方法 - Google Patents

Dnaメチル化検出用試料の調製方法 Download PDF

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Publication number
JP2008212009A
JP2008212009A JP2007050748A JP2007050748A JP2008212009A JP 2008212009 A JP2008212009 A JP 2008212009A JP 2007050748 A JP2007050748 A JP 2007050748A JP 2007050748 A JP2007050748 A JP 2007050748A JP 2008212009 A JP2008212009 A JP 2008212009A
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Japan
Prior art keywords
biological sample
dna
dna methylation
sample pretreatment
solution
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Pending
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JP2007050748A
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English (en)
Japanese (ja)
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JP2008212009A5 (enExample
Inventor
Masahiro Kajita
昌裕 梶田
Ayako Sakai
綾子 酒井
Noriaki Yamamoto
憲明 山本
Hidemiki Ishihara
英幹 石原
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Sysmex Corp
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Sysmex Corp
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Application filed by Sysmex Corp filed Critical Sysmex Corp
Priority to JP2007050748A priority Critical patent/JP2008212009A/ja
Priority to CNA2008100083371A priority patent/CN101255476A/zh
Priority to US12/038,493 priority patent/US20080206772A1/en
Priority to EP08152040A priority patent/EP1964934A1/en
Publication of JP2008212009A publication Critical patent/JP2008212009A/ja
Publication of JP2008212009A5 publication Critical patent/JP2008212009A5/ja
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2007050748A 2007-02-28 2007-02-28 Dnaメチル化検出用試料の調製方法 Pending JP2008212009A (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2007050748A JP2008212009A (ja) 2007-02-28 2007-02-28 Dnaメチル化検出用試料の調製方法
CNA2008100083371A CN101255476A (zh) 2007-02-28 2008-02-26 用于制备检测dna甲基化试样的试样处理液和试剂盒
US12/038,493 US20080206772A1 (en) 2007-02-28 2008-02-27 Sample treatment solution and reagent kit for preparing sample for detecting dna methylation
EP08152040A EP1964934A1 (en) 2007-02-28 2008-02-28 Sample treatment solution and reagent kit for preparing sample for detecting DNA methylation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2007050748A JP2008212009A (ja) 2007-02-28 2007-02-28 Dnaメチル化検出用試料の調製方法

Publications (2)

Publication Number Publication Date
JP2008212009A true JP2008212009A (ja) 2008-09-18
JP2008212009A5 JP2008212009A5 (enExample) 2008-11-06

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JP2007050748A Pending JP2008212009A (ja) 2007-02-28 2007-02-28 Dnaメチル化検出用試料の調製方法

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US (1) US20080206772A1 (enExample)
EP (1) EP1964934A1 (enExample)
JP (1) JP2008212009A (enExample)
CN (1) CN101255476A (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023535636A (ja) * 2020-07-30 2023-08-18 ケンブリッジ エピジェネティックス リミテッド 核酸解析のための組成物および方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法
AU2008334901A1 (en) * 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of lung carcinoma
WO2013067268A1 (en) 2011-11-03 2013-05-10 Tripath Imaging, Inc. Methods and compositions for preparing samples for immunostaining
CN111235227A (zh) * 2020-03-31 2020-06-05 西安天隆科技有限公司 一种游离dna提取及甲基化转化方法、试剂及试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
US7718364B2 (en) * 2003-03-25 2010-05-18 John Wayne Cancer Institute DNA markers for management of cancer
JP4166725B2 (ja) 2003-07-31 2008-10-15 独立行政法人科学技術振興機構 メチル化dna検出方法
JP4435174B2 (ja) * 2003-12-02 2010-03-17 エフ.ホフマン−ラ ロシュ アーゲー 重亜硫酸塩処理の改良された方法
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
EP1632578A1 (en) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023535636A (ja) * 2020-07-30 2023-08-18 ケンブリッジ エピジェネティックス リミテッド 核酸解析のための組成物および方法

Also Published As

Publication number Publication date
CN101255476A (zh) 2008-09-03
EP1964934A1 (en) 2008-09-03
US20080206772A1 (en) 2008-08-28

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