CN101250068A - Method for producing photosynthetic bacterium biological fertilizer by using livestock house sewage water - Google Patents
Method for producing photosynthetic bacterium biological fertilizer by using livestock house sewage water Download PDFInfo
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- CN101250068A CN101250068A CNA2008100354693A CN200810035469A CN101250068A CN 101250068 A CN101250068 A CN 101250068A CN A2008100354693 A CNA2008100354693 A CN A2008100354693A CN 200810035469 A CN200810035469 A CN 200810035469A CN 101250068 A CN101250068 A CN 101250068A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
A method for using the sewage of livestock house to produce photosynthetic bacterial biological fertilizer belongs to the technical field of agriculture and environment sciences. The inventive method uses swine manure waste water as raw material and the method comprises pretreating swine manure waste water, separating solid and liqud, taking off supernatant, grafting photosynthetic bacterial strain on the supernatant, culturing at suitable temperature and light condition, leading in azotobacter, phosphorus bacterial and potassium bacterial at the later period of culture, and adding microelements needed by crops, to obtain photosynthetic bacterial biological fertilizer. The invention has simple rpocss, low device cost, high product additional value and resolved environment pollution problem of swine manure waste water, while the product can accelerate the growth of crops, improve crop product and yield. The inventive fertilzier is suitable for the crops as rice, vegetable and fruit tree.
Description
Technical field
The present invention relates to a kind of method of producing photosynthetic bacterium biological fertilizer, belong to the technical field of agricultural sciences and environmental science with livestock house sewage water.
Background technology
In the later stage eighties 20th century, China's livestock and poultry breeding industry has obtained developing rapidly, and huge variation has taken place for livestock and poultry cultivation scale, breeding way and distributed areas, and the problem of environmental pollution that it brings totally presents the trend of increase, degree aggravation and range extension.Province, city's investigation of 23 the mass-producing livestock and poultry in the whole nation being concentrated in 2000 according to State Environmental Protection Administration show that 1999, the quantum of output of ground feces of livestock and poultry was about 1,900,000,000 tons in China, is 2.4 times of industrial solid castoff annual emissions.Contain a large amount of organic pollutants in the feces of livestock and poultry, only one of COD just reaches 7,118 ten thousand tons, considerably beyond the industry and the COD summation of sanitary sewage pollutent
[1]The influence of its environmental pollution is increasing, has become three big sources of water environment pollution side by side with industrial sewage, sanitary sewage.Swine excrement sewage is high-concentration sewage, and it is the pig house transformation that main flow is administered technology, and the dirty shunting of rain is done separately wet.Urine reaches dashes house water solid-liquid separation, solid-state part is produced organic fertilizer after with argol compost fermentation together, liquid portion anaerobic fermentation natural pond, its mixed solution is again through solid-liquid separation, the natural pond slag is made fertilizer utilization, natural pond liquid is through various aerobic biochemical treatment process, as discharging after sequencing batch active sludge (SBR), photosynthetic bacterium method (PSB), contact oxidation method, activated sludge process and the aerobic/anaerobic system art breading such as (A/O).But because livestock and poultry breeding industry is a small profit trade, improvement technology expensive, high working cost is difficult to accept.How the scientific utilization feces of livestock and poultry is turned waste into wealth it, is one of important topic of present developing agricultural recycling economy.
Summary of the invention
The objective of the invention is to release a kind of method of utilizing livestock house sewage water to produce multi-functional photosynthetic bacterium living beings fertilizer, this method can be produced the required photosynthetic bacterium biological fertilizer material of agricultural planting industry, has solved the pollution problem of sewage to environment simultaneously.
For realizing above purpose, the present invention adopts following technical scheme.This method is to be willing to material with the swine manure wastewater, pass through following steps: swine manure wastewater is carried out pre-treatment, solid-liquid separation, get supernatant liquor, supernatant liquor inoculation photosynthetic bacterium bacterial classification is cultivated under suitable temperature and illumination condition, and late stage of culture is introduced fixed nitrogen, phosphorus decomposing and potassium solubilizing bacteria, add the required trace element of farm crop, obtain the product photosynthetic bacterium biological fertilizer.
Now describe technical scheme of the present invention in detail.
A kind of method of utilizing livestock house sewage water to produce photosynthetic bacterium biological fertilizer is characterized in that the concrete operations step:
The pre-treatment of the first step livestock house sewage water
The livestock house sewage water of 1 part of weight is packed in the fermentation vat, anaerobically fermenting 48h-72h, solid-liquid separation, the supernatant liquor of 0.76 part of weight～0.84 part weight, standby;
Second goes on foot the cultivation of photosynthetic bacterium
The photosynthetic bacterium bacterial classification of 0.19 part of weight～0.21 part weight of inoculation in the supernatant liquor that the first step makes, the sodium acetate soln that adds 0.006 part of weight, the sodium-acetate that contains 0.001 part of weight in this solution, 20 ℃～30 ℃ and 1000lx～3000lx illumination were cultivated 7 days～10 days down, obtain the photosynthetic bacterium liquid fertilizer of 0.956 part of weight～1.056 part weight, wherein the density of photosynthetic bacterium is greater than 10
8MPNmL
-1
The production of the 3rd step composite microbic bacterial fertilizer
In the second photosynthetic bacterium liquid fertilizer that obtain of step, introduce vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria, the add-on of every kind of bacterium is between 0.019 part of weight～0.021 part weight, cultivated 2～3 days down, obtain the composite microbic bacterial fertilizer of 1.013 parts of weight～1.119 part weight for 20 ℃～30 ℃;
The production of the 4th step photosynthetic bacterium biological fertilizer
Each 0.002 part of weight of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid is dissolved in the warm water of 0.01 part of weight respectively, add the composite microbic bacterial fertilizer that the 3rd step obtained, mix evenly, obtain the photosynthetic bacterium biological fertilizer of 1.073 parts of weight～1.179 part weight.
Technical scheme of the present invention is further characterized in that, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, and their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillusmuciginosus.
Advantage of the present invention:
(1) the invention solves the pollution problem of livestock house sewage water to environment, realized turning waste into wealth, made raw material, produced photosynthetic bacterium biological fertilizer with livestock house sewage water, for the enforcement of Agricultural recycling economy provides good technical support, help the Sustainable development of livestock culture industry.
(2) technology of the present invention is simple, equipment cost is low, the added value height of product.
(3) product of the present invention's production can promote plant growth, improve agricultural output and quality.
Description of drawings
Fig. 1 is a process flow diagram of the present invention.
Embodiment
Now describe the function and the purposes of technical scheme of the present invention, product in conjunction with the embodiments in detail.All embodiment all operate according to the operation steps of production method mentioned above.Below each embodiment only enumerate crucial technical data.
Embodiment 1
In the first step, the anaerobically fermenting time is 48h, and supernatant liquor is 0.76 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.19 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 20 ℃ and 1000lx illumination were cultivated 7 days down, obtained 0.956 ton of photosynthetic bacterium liquid fertilizer; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.019 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillus muciginosus, cultivated 2 days down, obtain 1.013 tons of composite microbic bacterial fertilizers for 20 ℃; In the 4th step,, obtain 1.073 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
Embodiment 2
In the first step, the anaerobically fermenting time is 60h, and supernatant liquor is 0.8 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.2 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 25 ℃ and 2000lx illumination were cultivated 8.5 days down, obtained 1.006 tons of photosynthetic bacterium liquid fertilizers; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.02 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillus muciginosus, cultivated 2.5 days down, obtain 1.066 tons of composite microbic bacterial fertilizers for 25 ℃; In the 4th step,, obtain 1.126 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
Embodiment 3
In the first step, the anaerobically fermenting time is 72h, and supernatant liquor is 0.84 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.21 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 30 ℃ and 3000lx illumination were cultivated 10 days down, obtained 1.056 tons of photosynthetic bacterium liquid fertilizers; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.021 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillus muciginosus, cultivated 3 days down, obtain 1.119 tons of composite microbic bacterial fertilizers for 30 ℃; In the 4th step,, obtain 1.179 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
The function of the product that the present invention produces: use product photosynthetic bacteria of the present invention crop Bio-fertilizer can promote plant growth, improves crop yield and quality of agricultural product, can also improve crop Disease resistance. This is because described photosynthetic bacterium biological fertilizer both provided required little for crops Secondary element provides photosynthetic bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria etc. useful little for soil and crops again Biological. Photosynthetic bacteria is impelled crop growth, improves the micro-ecological environment of soil and crops, Strengthen the anti-environment-stress ability of crops, improve the quality of agricultural product; Phosphate solubilizing bacteria and potassium solubilizing bacteria Can improve the plant availability of P, K in soil and the fertilizer, improve simultaneously utilization rate of fertilizer.
The purposes of the product that the present invention produces: paddy rice, fruit and vegetables and so on farming especially suits to do Crop fertilizer.
Claims (4)
1, a kind of method of utilizing livestock house sewage water to produce photosynthetic bacterium biological fertilizer is characterized in that the concrete operations step:
The pre-treatment of the first step livestock house sewage water
The livestock house sewage water of 1 part of weight is packed in the fermentation vat, anaerobically fermenting 48h-72h, solid-liquid separation, the supernatant liquor of 0.76 part of weight～0.84 part weight, standby;
Second goes on foot the cultivation of photosynthetic bacterium
The photosynthetic bacterium bacterial classification of 0.19 part of weight～0.21 part weight of inoculation in the supernatant liquor that the first step makes, the sodium acetate soln that adds 0.006 part of weight, the sodium-acetate that contains 0.001 part of weight in this solution, 20 ℃～30 ℃ and 1000lx～3000lx illumination were cultivated 7 days～10 days down, obtain the photosynthetic bacterium liquid fertilizer of 0.956 part of weight～1.056 part weight, wherein the density of photosynthetic bacterium is greater than 10
8MPNmL
-1
The production of the 3rd step composite microbic bacterial fertilizer
In the second photosynthetic bacterium liquid fertilizer that obtain of step, introduce vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria, the add-on of every kind of bacterium is between 0.019 part of weight～0.021 part weight, cultivated 2～3 days down, obtain the composite microbic bacterial fertilizer of 1.013 parts of weight～1.119 part weight for 20 ℃～30 ℃;
The production of the 4th step photosynthetic bacterium biological fertilizer
Each 0.002 part of weight of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid is dissolved in the warm water of 0.01 part of weight respectively, add the composite microbic bacterial fertilizer that the 3rd step obtained, mix evenly, obtain the photosynthetic bacterium biological fertilizer of 1.073 parts of weight～1.179 part weight.
2, the method for utilizing livestock house sewage water to produce photosynthetic bacterium biological fertilizer according to claim 1 is characterized in that in the first step, the anaerobically fermenting time is 48h, and supernatant liquor is 0.76 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.19 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 20 ℃ and 10001x illumination were cultivated 7 days down, obtained 0.956 ton of photosynthetic bacterium liquid fertilizer; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.019 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillusmuciginosus, cultivated 2 days down, obtain 1.013 tons of composite microbic bacterial fertilizers for 20 ℃; In the 4th step,, obtain 1.073 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
3, the method for utilizing livestock house sewage water to produce photosynthetic bacterium biological fertilizer according to claim 1 is characterized in that in the first step, the anaerobically fermenting time is 60h, and supernatant liquor is 0.8 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.2 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 25 ℃ and 2000lx illumination were cultivated 8.5 days down, obtained 1.006 tons of photosynthetic bacterium liquid fertilizers; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.02 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillusmuciginosus, cultivated 2.5 days down, obtain 1.066 tons of composite microbic bacterial fertilizers for 25 ℃; In the 4th step,, obtain 1.126 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
4, the method for utilizing livestock house sewage water to produce photosynthetic bacterium biological fertilizer according to claim 1 is characterized in that in the first step, the anaerobically fermenting time is 72h, and supernatant liquor is 0.84 ton; In second step, the inoculum size of photosynthetic bacterium bacterial classification is 0.21 ton, and the add-on of sodium acetate soln is 0.006 ton, and the content of sodium-acetate is 0.001 ton in this solution, and 30 ℃ and 3000lx illumination were cultivated 10 days down, obtained 1.056 tons of photosynthetic bacterium liquid fertilizers; In the 3rd step, the add-on of vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria is 0.021 ton, vinelandii, phosphate solubilizing bacteria and potassium solubilizing bacteria are respectively nitrogen Zymomonas mobilis, phosphorus decomposing bacillus megaterium and colloid bacillus cereus, their formal name used at school is respectively Azomonas, Bacillus megatherium phosphaticoum and Bacillusmuciginosus, cultivated 3 days down, obtain 1.119 tons of composite microbic bacterial fertilizers for 30 ℃; In the 4th step,, obtain 1.179 tons photosynthetic bacterium biological fertilizer with each 0.002 ton of warm water that is dissolved in 0.01 ton respectively of zinc sulfate, copper sulfate, ferric sulfate, manganous sulfate and boric acid.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101913933A (en) * | 2010-08-12 | 2010-12-15 | 黑龙江省龙睦有机肥料有限责任公司 | Method for producing organic complex fertilizer through microbial solid fermentation |
| CN101823806B (en) * | 2009-03-03 | 2011-10-05 | 农业部沼气科学研究所 | Piggery waste water treatment method based on strong-thin shunt |
| CN102286540A (en) * | 2011-06-17 | 2011-12-21 | 河南农业大学 | Method for performing nitrogen fixation for straw anaerobic fermentation by utilizing organic wastewater |
| CN102399735A (en) * | 2011-12-21 | 2012-04-04 | 大连洪家畜牧有限公司 | Method for culturing photosynthetic bacteria by biogas liquid |
| CN102531715A (en) * | 2011-12-30 | 2012-07-04 | 刘金亮 | Method for producing environment-friendly liquid state compound fertilizer by using cattle excrement waste water |
| CN104211488A (en) * | 2013-08-29 | 2014-12-17 | 农业部规划设计研究院 | Preparation method of ecological liquid fertilizer taking anaerobic fermentation liquid as basic liquid |
| CN108358389A (en) * | 2018-03-09 | 2018-08-03 | 南通华多种猪繁育有限公司 | A kind of harmless processing of large-scale pig farm biogas slurry and utilization of resources technique |
| CN109553445A (en) * | 2019-01-04 | 2019-04-02 | 黄亦存 | Utilize the method for breeding wastewater production photosynthetic bacteria liquid fertilizer |
-
2008
- 2008-04-02 CN CNA2008100354693A patent/CN101250068A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101823806B (en) * | 2009-03-03 | 2011-10-05 | 农业部沼气科学研究所 | Piggery waste water treatment method based on strong-thin shunt |
| CN101913933A (en) * | 2010-08-12 | 2010-12-15 | 黑龙江省龙睦有机肥料有限责任公司 | Method for producing organic complex fertilizer through microbial solid fermentation |
| CN101913933B (en) * | 2010-08-12 | 2012-10-03 | 黑龙江省龙睦有机肥料有限责任公司 | Method for producing organic complex fertilizer through microbial solid fermentation |
| CN102286540A (en) * | 2011-06-17 | 2011-12-21 | 河南农业大学 | Method for performing nitrogen fixation for straw anaerobic fermentation by utilizing organic wastewater |
| CN102286540B (en) * | 2011-06-17 | 2013-03-20 | 河南农业大学 | Method for performing nitrogen fixation for straw anaerobic fermentation by utilizing organic wastewater |
| CN102399735A (en) * | 2011-12-21 | 2012-04-04 | 大连洪家畜牧有限公司 | Method for culturing photosynthetic bacteria by biogas liquid |
| CN102531715A (en) * | 2011-12-30 | 2012-07-04 | 刘金亮 | Method for producing environment-friendly liquid state compound fertilizer by using cattle excrement waste water |
| CN104211488A (en) * | 2013-08-29 | 2014-12-17 | 农业部规划设计研究院 | Preparation method of ecological liquid fertilizer taking anaerobic fermentation liquid as basic liquid |
| CN108358389A (en) * | 2018-03-09 | 2018-08-03 | 南通华多种猪繁育有限公司 | A kind of harmless processing of large-scale pig farm biogas slurry and utilization of resources technique |
| CN109553445A (en) * | 2019-01-04 | 2019-04-02 | 黄亦存 | Utilize the method for breeding wastewater production photosynthetic bacteria liquid fertilizer |
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Open date: 20080827 |