A kind of organic waste water that utilizes carries out the method that fixed nitrogen is used for stalk anaerobic fermentation
Technical field
The invention belongs to the comprehensive utilization of resources technical field, be specifically related to a kind of organic waste water that utilizes and carry out the method that fixed nitrogen is used for stalk anaerobic fermentation.
Background technology
China is a large agricultural country, has abundant stalk resource, but along with the fast development of economy, former as life in the countryside with can and the agricultural crop straw of animal feed progressively become agricultural wastes, its improper disposal has caused serious environmental pollution.In recent years, along with becoming increasingly conspicuous of the in short supply and environmental problem of fossil energy, become the important technology mode of alternative energy development as main biomass energy take stalk.In different kind organism mass-energy technology, straw methane fermentation not only can be converted into stalk the Gas Energy Source of cleaning, and the product after the fermentation also can be used as organic fertilizer and returns the field, realizes the benign cycle of stalk resource.But stalk is rich carbon biology plastid, and the straw methane fermentation microorganism needs a certain amount of nitrogenous source to finish the growth metabolism of self in anaerobic fermentation process, and pure stalk ferments and often causes normally fermentation gas of the excessive phenomenon of acid because lacking nitrogenous source.In order to remedy the problem of nitrogenous source deficiency in the stalk, present straw methane fermentation engineering in general all very important person satisfies the normal growth of anaerobically fermenting microorganism for increasing nitrogenous source, then replenish essential nitrogenous source by adding feces of livestock and poultry in the livestock and poultry breeding industry developed regions, then directly use as a supplement nitrogenous source of bicarbonate of ammonia, urea in the area that lacks aquaculture, caused the very large wasting of resources.
Photosynthetic bacterium is the archeobacteria that a class has special growth metabolism system, it can carry out the growth metabolism activity under the varying environment condition, wherein its situation under anaerobism illumination and nutritional condition nitrogen stress is the required nitrogenous source of self growth metabolism with the simple substance nitrogen transformation, and preserves with tropina and thalline pigment form.Fixation in Photosynthetic is a kind of nitrogen stress pathways metabolism of photosynthetic bacterium self nitrogenase, utilize this Characteristics of Nitrogen Fixation of photosynthetic bacterium just in the air abundant nitrogen resource conversion reduce the production run cost for organonitrogen can reduce its anaerobic fermentation process to the needs of artificial chemistry nitrogenous source for the carbon source of stalk anaerobic fermentation necessity.
Summary of the invention
The organic waste water that utilizes that the invention provides a kind of save energy carries out the method that fixed nitrogen is used for stalk anaerobic fermentation.
The present invention is by the following technical solutions:
A kind of organic waste water that utilizes carries out the method that fixed nitrogen is used for stalk anaerobic fermentation, may further comprise the steps: (1) utilizes the nitrogen product of air after the deoxidation reactor deoxidation treatment as initial nitrogenous source; (2) in organic waste water, inoculate photosynthetic bacterium, illumination cultivation in the atmosphere of nitrogen, it is the organonitrogen product that the liquid after the cultivation is got supernatant liquor through centrifugation; (3) add in the stalk anaerobic fermentation process as nitrogenous source after the deactivation of organonitrogen product, 100 gram stalks add the organonitrogen product of 400-800mL.
Photosynthetic bacterial load is the 5-10% of organic waste water volume when inoculating photosynthetic bacterium in the described step (2), and the organic waste water bottom passes into nitrogen makes the air pressure in the reactor reach 0.1Mpa; The time of illumination cultivation is no less than 96h, and the controlled light degree is 2000-3000 Lx, and culture temperature is 25-30 ℃.
The preparation process of nitrogen product is in the described step (1): be that the organic waste water of 4000-6000mg/L is sent in the deoxidation reactor with COD; The aerobic activated sludge that adds organic waste water quality 1/15-1/20 in the deoxidation reactor; Pass into air by deoxidation reactor bottom, air in deoxidation reactor from bottom to top in the flow process oxygen participate in reaction and be consumed, the gas of discharging deoxidation reactor is removed dry nitrogen product behind the carbonic acid gas.
Described deoxidation reactor comprises housing, housing bottom is provided with aerator, the import of aerator is connected with air intake duct, and the vertical interleaved of inner walls of aerator top is provided with downward-sloping and acclivitous traverse baffle, leaves the space between the far-end of traverse baffle and the relative inner walls; Case top is provided with spray thrower, and the import of spray thrower is connected with housing bottom by circulation tube, and the ingress of circulation tube is provided with recycle pump, and case top is provided with escape pipe, and housing top is provided with water inlet pipe, and housing bottom is skewed, and the housing bottom surface is provided with sewage draining exit.
Housing is provided with upflow tube; Water inlet pipe is located at from top to bottom between second traverse baffle and the 3rd traverse baffle.
The present invention utilizes photosynthetic bacterium to utilize the nitrogen fixation of self nitrogenase that airborne nitrogen transformation is tropina and thalline pigment under the anaerobism illumination condition nutritive substance in the organic waste water, thereby transform airborne simple substance nitrogen fixing with the organic form, then supply with the stalk anaerobic fermentation process to the demand of nitrogenous source as nitrogenous source, alleviated in the present stalk fermentation process and needed to add bicarbonate of ammonia, the very large wasting of resources that the additional nitrogenous source such as urea causes, also make organic waste water obtain re-using, avoid organic waste water directly to discharge the pollution that causes environment and destroy.The present invention is simple to the fixed nitrogen technique of organic waste water, and its prime cost is the illumination of illumination cultivation to power consumption, makees lamp as adopting sunlight, and the proper extension incubation time remedies sunlight periodical change impact and then can greatly reduce production costs.
The present invention carries out will carrying out sterilising treatment before the fixed nitrogen processing to organic waste water, prevents living contaminants, reduces simultaneously atmospheric oxygen to the restraining effect of photosynthetic bacterium.The present invention can not directly utilize air as the nitrogenous source of organic waste water metabolic reaction, this is because oxygen has restraining effect to nitrogenase in the air, therefore the nitrogen that adopts must be the air after the deoxidation, if when micro amount of oxygen exists, should increase incubation time, so that at first carrying out the micro amount of oxygen of aerobic respiration effect consumption existence, photosynthetic bacterium then changes the fixed nitrogen metabolism over to.
When utilizing organic waste water to obtain nitrogen product among the present invention, organic waste water COD need to control, and this is because then can not eliminate the oxygen that contains in the air fully when organic waste water COD crosses when hanging down, and a large amount of CO is then arranged when organic waste water COD is too high
2, the new foreign gas such as CO generates, not only increasing the consumption that absorbs alkali lye also affects the purity that obtains nitrogen.COD is so that the catabolism of organic waste water rests on aerobic and little aerobic level in control, avoids the generation of organic matter how assorted gas under anaerobic state.Utilize organic waste water to obtain that the method for nitrogen product is simple, energy consumption is low, when obtaining nitrogen, organic waste water also had certain cleaning action, utilize that nitrogen gas concn (volume percent) can reach about 95% in the gas that the inventive method obtains, also contain oxygen and the CO (carbon monoxide converter) gas of a small amount of concentration in the aerogenesis, can be used as the precursor gas of obtaining high pure nitrogen.
It is simple to utilize organic waste water to obtain the used deoxidation reactor structure design of nitrogen product, rationally distributed, traverse baffle is set, can makes air in reactor, realize abundant baffling from bottom to top, guarantee that airborne oxygen is farthest fallen by the organic matter degradation metabolic exhaustion.
Description of drawings
Fig. 1 is the structural representation of used deoxidation reactor among the present invention;
The structural representation of system when Fig. 2 is the deoxidation reactor use.
Embodiment
Test adopts the maltose factory production waste water to make sample, and as photosynthetic bacterium nutrition substrate, a part is used for air deoxidation medium and uses a part behind ultraviolet sterilization.
Deoxidation reactor, as shown in Figure 1, comprise housing 1, housing 1 bottom is provided with aerator 2, the import of aerator 2 is connected with air intake duct 3, the vertical interleaved of housing 1 inwall of aerator 2 tops is provided with downward-sloping and acclivitous traverse baffle 4, leaves the space between the far-end of traverse baffle 4 and relative housing 1 inwall; Housing 1 top is provided with spray thrower 5, the import of spray thrower 5 is connected with housing 1 bottom by circulation tube 6, the ingress of circulation tube 6 is provided with recycle pump 7, in the operational process, recycle pump 7 is circulated to spray thrower 5 with the organic waste water of bottom, so that the organic waste water in the housing 1 constantly circulates, unnecessary waste water is discharged by upflow tube 11; Housing 1 top is provided with escape pipe 8, housing 1 top is provided with water inlet pipe 9 between second traverse baffle 4 and the 3rd traverse baffle 4 from top to bottom, constantly is supplemented with organic waste water through water inlet pipe 9, and housing 1 is provided with upflow tube 11, housing 1 bottom is skewed, and housing 1 bottom surface is provided with sewage draining exit 10.
When deoxidation reactor uses, as shown in Figure 2, the air intake duct 3 of deoxidation reactor is connected with gas-holder 20, and gas-holder 20 is connected with air compressor 23, the escape pipe 8 of deoxidation reactor is connected with alkaline respiratory box 21, and the pneumatic outlet of alkaline respiratory box 21 is connected with dewatering box 22.
The organic waste water adjustment COD that is used for air deoxidation medium part is 5550mg/L, in water inlet pipe 9 enters the housing 1 of deoxidation reactor; Make water level reach about 2/3 of the housing 1 interior degree of depth, add the aerobic activated sludge of organic waste water quality 1/20 in the deoxidation reactor; Utilize air compressor 23 that air is pressed into gas-holder 20, air is scattered in the bottom of deoxidation reactor through inlet pipe 3, aerator 2, and compile at the relatively high end of traverse baffle 4, increase along with air flow, the air that compiles spreads to traverse baffle 4 lower end diffusion and then continuous making progress, air under the effect of traverse baffle 4 step by step to the motion of the top of deoxidation reactor, air in the process of baffling, contained O
2Participate in organic DeR and reach the effect of elimination, final gas is discharged by the escape pipe 8. at housing 1 top.Major part be nitrogen in the air that escape pipe 8 is discharged, and the carbonic acid gas that produces with organic matter degradation that contains also that itself contains in the air continues to pass into alkaline respiratory box 21 removal carbonic acid gas, obtains nitrogen product after dewatering finally by dewatering box 22.The composition that records in the nitrogen product is: N
2: 97.2%, O
2: 0.8%, CO
2: 0%, CO:0.4%, the COD that records in the organic waste water that upflow tube 11 flows out is 4100mg/L.In the operational process, recycle pump 7 is circulated to spray thrower 5 with the organic waste water of bottom through circulation tube 6, so that the organic waste water in the housing 1 constantly circulates, constantly is supplemented with organic waste water by water inlet pipe 9, and unnecessary waste water is discharged by upflow tube 11; After operation for some time, close air, recycle pump 7, open sewage draining exit 10 and carry out blowdown, remove the mud granule in the reactor, begin the operation of subsequent processing after the cleaning.
Organic waste water as photosynthetic bacterium nutrition substrate behind ultraviolet sterilization adds in the reactor; (2) the access inoculum size is the photosynthetic bacterium of organic waste water volume 5% in the reactor, and photosynthetic bacterium is the mixed bacterium of Rhodopseudomonas spheroides and Crimson rhodospirillum; (3) bottom of reactor passes into the nitrogen that deoxidation reactor dewatering box 22 discharges and makes the air pressure in the reactor reach 0.1Mpa; (4) adopt incandescent light that organic waste water is carried out illumination cultivation 96h, adjusting incandescent light and reactor is 2000 Lx apart from the controlled light degree, and culture temperature is 25 ℃; (5) cultivate finishing liquid in the post-reactor, to get supernatant liquor through centrifugation be the organonitrogen product; (6) the organonitrogen product is cooled to room temperature through 100 ℃ of sterilizations after 5 minutes, then the organonitrogen product is added in the stalk anaerobic fermentation process as nitrogenous source, and 100 gram stalks add the organonitrogen product of 400-800mL.