CN102399735A - Method for culturing photosynthetic bacteria by biogas liquid - Google Patents
Method for culturing photosynthetic bacteria by biogas liquid Download PDFInfo
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- CN102399735A CN102399735A CN2011104309010A CN201110430901A CN102399735A CN 102399735 A CN102399735 A CN 102399735A CN 2011104309010 A CN2011104309010 A CN 2011104309010A CN 201110430901 A CN201110430901 A CN 201110430901A CN 102399735 A CN102399735 A CN 102399735A
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Abstract
The invention relates to a method for culturing photosynthetic bacteria by biogas liquid. The method comprises the following steps of: adding 0.3 weight percent of sodium diacetate, 0.1 percent of ammonium bicarbonate, 0.4 weight percent of sodium bicarbonate and 0.1 weight percent of potassium sulfate into the biogas liquid until the pH value is between 7.5 and 8.5 to prepare a culture solution; and adding strains, which have the same weight as that of the culture solution, of the photosynthetic bacteria into the culture solution, inoculating, performing culture on the culture solution at the temperature of between 20 and 30 DEG C in a culture chamber, arranging a 60 W filament lamp at the position 20 to 30 centimeters away from a culture utensil for irradiating, and culturing for 72 hours to obtain bacterial liquid, so that the inoculated culture solution is deep red. The method has the advantages that the biogas liquid is used as a culture substrate at low-cost environment, and the cultured bacterial liquid of the photosynthetic bacteria has effects of improving the productivity of animals, enhancing immunity, eliminating peculiar smells of livestock and poultry houses and the like.
Description
Technical field
The invention belongs to biological technical field, relate in particular to a kind of method of cultivating photosynthetic bacterium.
Background technology
Photosynthetic bacterium (PBS) be one type with light as obnoxious flavour and toxin such as the energy, the organism that can under anaerobism illumination or aerobic dark condition, utilize occurring in nature, sulfide, ammonia-state nitrogen, methyl alcohol, indoles, propylene, carry out photosynthetic mikrobe as the hydrogen donor carbon source of holding concurrently.Be converted into useful biomass cells and since the thalline of photosynthetic bacteria contain the various vitamin B group of abundant nutrient, somatomedin, physiologically actives such as ubiquinone and antiviral agent, have balance Digestive tract ecotope; Remarkable the acting on such as quality that promote the performance of breeding performonce fo animals and improve livestock product have the ability degraded of the harmful bacteria growing of very strong inhibition and eliminate hazardous compound, improve gastroenteric environment; Antiviral substance; The control infectious disease of the digestive tract, the immune system activity of promotion livestock and poultry has promotion and grows; Improve production performance; Improving effect such as animal products quality significantly, is a kind of safety, pollution-free green, environment-friendly feed additive of drug residue free, is used widely.Because the price of photosynthetic bacterium is higher, so the application of photosynthetic bacterium is difficult to obtain promote.
At present, photosynthetic bacterium mainly contains dynamically and leaves standstill two kinds of cultural methods, and culture apparatus has open and airtight two types.The transparent vessel of domestic basic employing leave standstill best cultivation under illumination.For photosynthetic substratum; Compounds such as carbon, nitrogen and phosphorus have generally been comprised; Because carbon and nitrogen account for the large percentage of photosynthetic bacterium dry weight because substratum is nitrogenous in forming, the selection of carbon cpd with confirm in photosynthetic bacteria culture medium, to occupy important status.Therefore, confirming both to have supported that photosynthetic bacterium grew fast, can keep the stable nitrogenous source of culture PH, carbon source again simultaneously, is the key that obtains the high density photosynthetic bacterium.
Natural pond liquid is that the excrement soiled ends is crossed the microbiological anaerobic fermentation, produces the by product of biogas, a kind of high-quality fertilizer.In view of natural pond liquid contains abundant nitrogen, carbon nutrition and multivitamin, trace element and biologically active substance, thereby can become the beneficial microorganism substratum.
Summary of the invention
The object of the present invention is to provide not only and can under the lower cost environment, utilize natural pond liquid, and the photosynthetic bacteria liquid of turning out can play a kind of method of cultivating photosynthetic bacterium of effects such as improving breeding performonce fo animals, strengthening immunity, elimination livestock and poultry house peculiar smell in application as culture medium.
A kind of method of using natural pond liquid cultivation photosynthetic bacterium that the present invention proposes, its technical scheme is following:
Step 1: natural pond liquid extracts
The excrement of getting healthy livestock and poultry is dirty, makes the excrement soiled ends cross the anaerobically fermenting more than 15 days, obtains defective material behind the anaerobically fermenting, again defective material is carried out solid-liquid separation, gets its supernatant after the separation, then accomplishes the extraction of natural pond liquid.
Step 2: inoculum preparation
In the natural pond liquid that above-mentioned steps 1 obtains, add weight ratio and be 3 ‰ sodium Diacetate, 1 ‰ bicarbonate of ammonia, 4 ‰ sodium hydrogencarbonate and 1 ‰ vitriolate of tartar, pH value is 7.5～8.5, then accomplishes the preparation of nutrient solution.
Step 3: inoculation culture
In the cultivation vessel (polyethylene bag or incubator) that the nutrient solution that above-mentioned steps 2 is obtained is packed into transparent, the photosynthetic bacterium stoste (bacterial classification) of weight such as in cultivating the inner nutrient solution of vessel, add, will cultivate vessel and seal to be placed on and cultivate in the culturing room; The temperature of culturing room remains on 20～30 ℃, and inoculation back nutrient solution is cultivated the incandescent light irradiation that vessel 20～30 centimeters are provided with 60W in distance when cultivating; Along with the prolongation of incubation time, postvaccinal nutrient solution transparence reduces, and color is deepened gradually; Cultivated 72 hours; Postvaccinal nutrient solution is scarlet, cultivates and accomplishes, and obtains bacterium liquid.
The bacterium liquid that utilizes such scheme to obtain contains the photosynthetic bacterium amount and reaches 30～5,000,000,000/ml, and can reach 30 days the storage period of bacterium liquid.And this bacterium liquid can carry out vaccinization and cultivate; As long as in bacterium liquid, add the nutrient solution that obtains by step 2 of equivalent; Cultivate according to the method for step 3 again, then can obtain more bacterium liquid, the vaccinization cultured continuously; Not only can guarantee the high cell concentration of photosynthetic bacterium, and greatly reduce production cost.
When the bacterium liquid that this programme obtains uses in the livestock-raising field, can add the interior drink-service of water of livestock and poultry, also can admix feed and feed; Consumption is following:
Broiler chicken: every 20～3,000,000,000/day; Laying hen: every 20～10,000,000,000/day; Pig: every 2000～6,000 hundred million/day.
The present invention compared with prior art has following advantage:
1, utilize natural pond liquid as culture medium, cost is low, and the height that produces effects is suitable for scale operation, helps promoting the development of green cultivation industry, to promoting resource recovery and having developed a circular economy very big meaning.
The bacterium liquid of 2, turning out can play effects such as improving breeding performonce fo animals, strengthening immunity, elimination livestock and poultry house peculiar smell in application, be a kind of efficient, safe, pollution-free, environmental and ecological type fodder additives.
Can promote breeding performonce fo animals to do following test once tame egg manufacturer to laying hen bacterium liquid: 7719 of the laying hens of selecting to be all for 36 ages in week are test group, and 7992 is control group.High bed is raised in cages, and supplies with 3.0 liters of photosynthetic bacteria bacterium liquid by every test chicken crowd, and every chicken supplies photosynthetic bacteria about 7,800,000,000 photosynthetic bacterias every day, and 56 days trial periods, test-results is that test group improves 2.1% than control group laying rate.Thus it is clear that, turn out to such an extent that bacterium liquid plays tangible effect to improving breeding performonce fo animals by the present invention.
Description of drawingsThe present invention does not have accompanying drawing.
EmbodimentEmbodiment 1
Step 1: natural pond liquid extracts
The excrement of getting healthy livestock and poultry is dirty, makes the excrement soiled ends cross 15 days anaerobically fermenting, obtains defective material behind the anaerobically fermenting, again defective material is carried out solid-liquid separation, gets its supernatant after the separation, then accomplishes the extraction of natural pond liquid.
Step 2: inoculum preparation
In the natural pond liquid that above-mentioned steps 1 obtains, add weight ratio and be 3 ‰ sodium Diacetate, 1 ‰ bicarbonate of ammonia, 4 ‰ sodium hydrogencarbonate and 1 ‰ vitriolate of tartar, pH value is 7.5, then accomplishes the preparation of nutrient solution.
Step 3: inoculation culture
The nutrient solution that above-mentioned steps 2 is obtained is packed in the transparent polyethylene bag, the photosynthetic bacterium stoste (bacterial classification) of weight such as in cultivating the inner nutrient solution of vessel, adds, and will cultivate vessel and seal to be placed on and cultivate in the culturing room; The temperature of culturing room remains on 25 ℃, and inoculation back nutrient solution is cultivated the incandescent light irradiation that vessel 25 centimeters are provided with 60W in distance when cultivating; Along with the prolongation of incubation time, postvaccinal nutrient solution transparence reduces, and color is deepened gradually; Cultivated 72 hours; Postvaccinal nutrient solution is scarlet, cultivates and accomplishes, and obtains bacterium liquid.
Claims (1)
1. use the method that natural pond liquid is cultivated photosynthetic bacterium for one kind, it is characterized in that:
Step 1: natural pond liquid extracts
The excrement of getting healthy livestock and poultry is dirty, makes the excrement soiled ends cross the anaerobically fermenting more than 15 days, obtains defective material behind the anaerobically fermenting, again defective material is carried out solid-liquid separation, gets its supernatant after the separation, then accomplishes the extraction of natural pond liquid;
Step 2: inoculum preparation
In the natural pond liquid that above-mentioned steps 1 obtains, add weight ratio and be 3 ‰ sodium Diacetate, 1 ‰ bicarbonate of ammonia, 4 ‰ sodium hydrogencarbonate and 1 ‰ vitriolate of tartar, pH value is 7.5～8.5, then accomplishes the preparation of nutrient solution;
Step 3: inoculation culture
In the cultivation vessel (polyethylene bag or incubator) that the nutrient solution that above-mentioned steps 2 is obtained is packed into transparent, the photosynthetic bacterium stoste (bacterial classification) of weight such as in cultivating the inner nutrient solution of vessel, add, will cultivate vessel and seal to be placed on and cultivate in the culturing room; The temperature of culturing room remains on 20～30 ℃, and inoculation back nutrient solution is cultivated the incandescent light irradiation that vessel 20～30 centimeters are provided with 60W in distance when cultivating; Along with the prolongation of incubation time, postvaccinal nutrient solution transparence reduces, and color is deepened gradually; Cultivated 72 hours; Postvaccinal nutrient solution is scarlet, cultivates and accomplishes, and obtains bacterium liquid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102943058A (en) * | 2012-11-19 | 2013-02-27 | 黑龙江省科学院微生物研究所 | Method for anaerobic shake culture of photosynthetic bacteria by utilizing biogas slurry |
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| CN101250068A (en) * | 2008-04-02 | 2008-08-27 | 上海市农业科学院 | Method for producing photosynthetic bacterium biological fertilizer by using livestock house sewage water |
| CN101891545A (en) * | 2010-07-15 | 2010-11-24 | 吴成祥 | Functional liquid bioorganic fertilizer and preparation method thereof |
| CN102212491A (en) * | 2011-03-29 | 2011-10-12 | 天津市农业生物技术研究中心 | Simple culture method of photosynthetic bacteria with high cell concentration |
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Patent Citations (3)
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| CN101250068A (en) * | 2008-04-02 | 2008-08-27 | 上海市农业科学院 | Method for producing photosynthetic bacterium biological fertilizer by using livestock house sewage water |
| CN101891545A (en) * | 2010-07-15 | 2010-11-24 | 吴成祥 | Functional liquid bioorganic fertilizer and preparation method thereof |
| CN102212491A (en) * | 2011-03-29 | 2011-10-12 | 天津市农业生物技术研究中心 | Simple culture method of photosynthetic bacteria with high cell concentration |
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| 《太阳能学报》 20051231 张全国等 猪粪污水浓度对球形红假单胞菌光合制氢的影响 806-810 1 第26卷, 第6期 * |
| 包有霞等: "沼泽红假单胞菌的培养和生产番茄红素的优化控制", 《食品与生物技术学报》, vol. 28, no. 5, 30 September 2009 (2009-09-30), pages 693 - 698 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102943058A (en) * | 2012-11-19 | 2013-02-27 | 黑龙江省科学院微生物研究所 | Method for anaerobic shake culture of photosynthetic bacteria by utilizing biogas slurry |
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Application publication date: 20120404 |