CN101084715A - Paris polyphylla var. pseudothibetica rhizome multiple shoot asexual reproduction method - Google Patents
Paris polyphylla var. pseudothibetica rhizome multiple shoot asexual reproduction method Download PDFInfo
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Abstract
The invention relates to a propagation method in the field of vegetative propagation, specially relates to a cluster bud propagation method of Paris polyphylla rhizomatic of Paridis. The invention is to provide a method with the advantages of simple operation, low cost, high propagation coefficient, rapid plumule growth, good seedling growth vigor, and no influence to the medicinal value of the propagating material to solve the problem of hard propagation of Paris polyphylla. The cluster bud propagation method of Paris polyphylla rhizom comprises selecting propagating material, processing method of the propagating material, processing time of the propagating material which can be performed all year around, inducing circumstance of cluster bud and managing, transplanting the seedling of cluster bud. Compared with the present technology, the invention has the advantages of simple operation, low cost, high propagation coefficient, good seedling growth vigor, and no influence to the medicinal value of the propagating material.
Description
Technical field
The present invention relates to the propagation method in plant asexual multiplication technology field, particularly the bud asexual reproduction method of growing thickly of the long connective rhizoma paris rhizome of paris plant.
Technical background
The long connective Paris polyphylla of paris plant (Li Heng edits paris plant: Paris polyphylla Smith var.pseudothibeticaH.Li, Li Heng. paris plant [M]. and Beijing: Science Press, 1998.The Hubei flora: Paris polyphylla Smith var.thibetica (Franch.) Hara) can to make Paris polyphylla medicinal for rhizome, is China's conventional Chinese medicine material.Its main chemical compositions contains flea and stops glycosides, Dioscin, steroidal and alkaloid.Have clearing heat and detoxicating, swelling and pain relieving, the function of cool liver arresting convulsion.It is swollen to be mainly used in the furunculosis carbuncle, abscess of throat, and venomous snake bite, traumatic injury, convulsion, antibacterial, anti-early pregnancy can also anticancerly stop blooding, eliminate the phlegm.Be widely used in Chinese patent drug and new drug in recent years, because the huge economic value of paris plant in exploitation, so the research of paris plant is paid attention to always very much.
In recent years, people attach great importance to the man planting technology research of the wild commentaries on classics of paris plant, and have set up the planting base of small size.But because provenance is few, seminal propagation needed just emerge in 2 years, and the seeding stage is long, and planting percent is low, and its tissue culture quick breeding Study on Technology is yet not success.Though vegetative propagation research report is more, the method that adopts all is with terminal bud, or rhizome is cut into several sections makes propagating materials, some is HORMONE TREATMENT in addition also, but generally each stem section can only produce 1～3 young shoot that growth potential is more weak, and not only the rudiment number is few, and reproduction coefficient is low; And the rhizome that is cut into several sections has also been lost medical value.Therefore solving its propagation technique difficult problem, is the key of Paris polyphylla medicinal material large-scale production.
Summary of the invention:
The objective of the invention is to the propagation technique difficult problem at the large-scale production of Paris polyphylla medicinal material, provide a kind of simple to operate, cost is low, the reproduction coefficient height, and the young shoot growth is fast, and the seedling growing way is prosperous, does not influence the method for the medical value of former propagating materials substantially.
Technical scheme of the present invention is: the bud propagation method of growing thickly of the long connective rhizoma paris rhizome of a kind of paris plant is characterized in that:
(a), the selection of propagating materials: get the rhizome of the perennial band bud of long connective Paris polyphylla, as the propagating materials of inducing clumping bud;
(b), the processing method of propagating materials: remove the soil on the rhizome, between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections behind the bud, get a section of the band bud and make propagating materials with cutter;
(c), the processing time of propagating materials: all can carry out the whole year;
(d), the induced bundle environment and the management of sprouting: the propagating materials after the processing will in time be planted into the field and be chosen the Seedlings nursery of fertile sand, sandy loam or the Seedlings nursery of forming with vermiculite matrix; The Seedlings nursery environmental requirement shelters from heat or light, ventilates, cools, moistening;
(e), the grow thickly transplantation technique of bud seedling:
Transplanting period: segment was handled the back 2～4 years;
Transplanting time: the jaundice of overground part plant is about to seedling or has just fallen Miao Houshi;
Transplanting method: should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged; The bud head upwards when planting.
The present invention has compared with the prior art: simple to operate, cost is low; The reproduction coefficient height, a propagating materials can form 5～30 (average 9) buds of growing thickly; The young shoot growth is fast, and the seedling growing way is prosperous; Substantially do not influence the medical value of former propagating materials.And being rhizome with multiple in the congener (or mutation), conventional method is cut into several sections, every section as a propagating materials, the methods such as use hormone that also have are handled propagating materials, but every section also can only produce 1～3 sprouting, and sprouting differentiation is slow, a little less than the growing way, and be cut into several sections rhizome and lost medical value.Therefore, making an accurate selection of long connective Paris polyphylla and segment position is the key technology of this invention.
Embodiment
The present invention is further illustrated below in conjunction with embodiment:
Example 1: the selection of propagating materials: choose the rhizome of perennial band bud of the long connective Paris polyphylla of paris plant of artificial planting, as the propagating materials of inducing clumping bud.
The processing method of propagating materials: the propagating materials of choosing removes the soil on the rhizome, but can not hinder bud, will keep when the overground part plant is arranged, and between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections with scraper behind the bud again.Make propagating materials for one section of band bud or plant, another section is used as medicine.Propagating materials wound after the segment dips in fresh ash.
The processing time of propagating materials: the processing time is in mid-April, on the occasion of plant overground part growing period.
Environment that induced bundle is sprouted and management: the propagating materials after the processing is in time planted into Seedlings nursery.Selection have cool, fertile farmland that the height above sea level 1600m of moistening physioclimate area soil property is sandy loam makes Seedlings nursery; Set up shade again, guarantee ventilation, shading, cooling.Hold gutter and railway carriage or compartment ditch successfully, do ditch cleaning row stain well, handle and have only the extended soil of terminal bud on the part propagating materials then, second and third successively between on differentiate the part sprouting, per hectare fall behind the seedling winter with the 45000 kilograms of works of fertilizer that the become thoroughly decomposed fertilizer of surviving the winter, spread fertilizer over the fields, cover the 1cm thick soil stratum again in the railway carriage or compartment face.Only grow 1～2 seedling on each propagating materials of 1 year spring, to mid or late October each propagating materials, differentiate 4～30 young shoots, except that growing 1～2 seedlings, can grow the seedling of 4～30 1～4 leaves on the 3rd year each propagating materials of spring.
The grow thickly transplanting of bud seedling: handle the back in segment and get transplantation of seedlings in October, the 3rd, when getting seedling propagating materials is dug from Seedlings nursery together with seedling, seedling is broken into two with one's hands from propagating materials again, attention does not damage root system.
Transplanting method:
Should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged.Should not be chosen in alkaline soil or the clay and cultivate.After choosing the plot, soil is ploughed deeply 20～25cm, apply decomposed dung, compost 37500～45000kg or the cake-shaped rape seed dregs 1500kg that becomes thoroughly decomposed, plough under and make base manure in the soil in conjunction with whole ground per hectare autumn and winter.Shallow plowing before the plantation harrow brokenly, flattens bedding, the wide 1.2～1.5m of furrow, and the furrow face is turtleback shape, furrow furrow width 30cm, furrow ditch depth 10cm.
Transplant in the land for growing field crops by distance between rows and hills 20～25cm during transplanting, irrigate normal root water after planting, in order to surviving.The bud head makes progress when planting, and root is unfolded in ditch, the thick 4～5cm of earthing.Requirement is with digging with planting.
Example 2: the selection of propagating materials: choose the rhizome of the perennial band bud of the long connective Paris polyphylla of wild paris plant, as the propagating materials of inducing clumping bud.
The processing method of propagating materials: the propagating materials of choosing removes the soil on the rhizome, but can not hinder bud, will keep when the overground part plant is arranged, and between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections with scraper behind the bud again.Make propagating materials for one section of band bud or plant, another section is used as medicine.Propagating materials wound after the segment dips in fresh ash.
The processing time of propagating materials: the processing time is in mid-August, on the occasion of plant overground part growing period.
Environment that induced bundle is sprouted and management: the propagating materials after the processing is in time planted into Seedlings nursery.Propagating materials after the processing is in time planted into Seedlings nursery.Selection have cool, fertile farmland that the height above sea level 1600m of moistening physioclimate area soil property is sandy loam makes Seedlings nursery; Set up shade again, guarantee ventilation, shading, cooling.Hold gutter and railway carriage or compartment ditch successfully, do ditch cleaning row stain well, handle and have only the extended soil of terminal bud on the part propagating materials then, second and third successively between on differentiate part sprouting original hase, the per hectare of falling winter behind the seedling makes to survive the winter fertilizer with the fertilizer 45000kg that becomes thoroughly decomposed, and spreads fertilizer over the fields in the railway carriage or compartment face, covers the 1cm thick soil stratum again.Only grow 1 to 2 seedling on each propagating materials of 1 year spring, to mid or late October each propagating materials, differentiate 4～30 young shoots, except that growing 1～2 seedlings, can grow the seedling of 4～30 1～4 leaves on the 3rd year each propagating materials of spring.
The grow thickly transplanting of bud seedling: handle the back in segment and get transplantation of seedlings in October, the 3rd, when getting seedling propagating materials is dug from Seedlings nursery together with seedling, seedling is broken into two with one's hands from propagating materials again, attention does not damage root system.
Transplanting method:
Should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged.Should not be chosen in alkaline soil or the clay and cultivate.After choosing the plot, soil is ploughed deeply 20～25cm, apply decomposed dung, compost 37500～45000kg or the cake-shaped rape seed dregs 1500kg that becomes thoroughly decomposed, plough under and make base manure in the soil in conjunction with whole ground per hectare autumn and winter.Shallow plowing before the plantation harrow brokenly, flattens bedding, the wide 1.2～1.5m of furrow, and the furrow face is turtleback shape, furrow furrow width 30cm, furrow ditch depth 10cm.
Transplant in the land for growing field crops by distance between rows and hills 20～25cm during transplanting, irrigate normal root water after planting, in order to surviving.The bud head makes progress when planting, and root is unfolded in ditch, the thick 4～5cm of earthing.Requirement is with digging with planting.
Field management: before emerge, need to keep the furrow face not have weeds after the transplanting, also will in time weed a garden and loosen the soil after emerging, accomplish diligent hoe, shallow hoe, avoid hindering root and influence its growth potential.
Example 3: the selection of propagating materials: the rhizome propagating materials of perennial band bud of getting the long connective Paris polyphylla of paris plant of wild or artificial planting.
The processing method of propagating materials: the propagating materials of choosing removes the soil on the rhizome, but can not hinder bud, will keep when the overground part plant is arranged, and between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections with scraper behind the bud again.Make propagating materials for one section of band bud or plant, another section is used as medicine.Propagating materials after the segment is placed slightly, treats that wound is dried slightly.
The processing time of propagating materials: the processing time is in mid-August, on the occasion of plant overground part growing period.
Environment that induced bundle is sprouted and management: the propagating materials after the processing is in time planted into the vermiculite matrix Seedlings nursery, and Seedlings nursery is the greenhouse that the device that shelters from heat or light is arranged, and guarantees to lower the temperature with the discontinuity spray humidification in ventilation, shading, summer.The thick matrix that contains vermiculite of propagating materials loam cake 2～3cm after the processing.
The grow thickly transplanting of bud seedling: because the greenhouse is long vegetative period, growth conditions is good, helps growth of seedling, handles back in October, second in segment and transplants, and does sth. in advance the garden than the field, and economic benefit is better.
Transplanting method: should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged.Should not be chosen in alkaline soil or the clay and cultivate.After choosing the plot, soil is ploughed deeply 20～25cm, apply decomposed dung, compost 37500～45000kg or the cake-shaped rape seed dregs 1500kg that becomes thoroughly decomposed, plough under and make base manure in the soil in conjunction with whole ground per hectare autumn and winter.Shallow plowing before the plantation harrow brokenly, flattens bedding, the wide 1.2～1.5m of furrow, and the furrow face is turtleback shape, furrow furrow width 30cm, furrow ditch depth 10cm.
Transplant in the land for growing field crops by distance between rows and hills 20～25cm during transplanting, irrigate normal root water after planting, in order to surviving.The bud head makes progress when planting, and root is unfolded in ditch, the thick 4～5cm of earthing.Requirement is with digging with planting.
Field management: before emerge, need to keep the furrow face not have weeds after the transplanting, also will in time weed a garden and loosen the soil after emerging, accomplish diligent hoe, shallow hoe, avoid hindering root and influence its growth potential.
Claims (3)
1, the bud propagation method of growing thickly of the long connective rhizoma paris rhizome of a kind of paris plant is characterized in that:
(a), the selection of propagating materials: get the rhizome of the perennial band bud of long connective Paris polyphylla, as the propagating materials of inducing clumping bud;
(b), the processing method of propagating materials: remove the soil on the rhizome, between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections behind the bud, get a section of the band bud and make propagating materials with cutter;
(c), the processing time of propagating materials: all can carry out the whole year;
(d), the induced bundle environment and the management of sprouting: the propagating materials after the processing will in time be planted into the field and be chosen the Seedlings nursery of fertile sand, sandy loam or the Seedlings nursery of forming with vermiculite matrix; The Seedlings nursery environmental requirement shelters from heat or light, ventilates, cools, moistening;
(e), the grow thickly transplantation technique of bud seedling:
Transplanting period: segment was handled the back 2～4 years;
Transplanting time: the jaundice of overground part plant is about to seedling or has just fallen Miao Houshi;
Transplanting method: select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged.
2, want the bud propagation method of growing thickly of 1 described long connective rhizoma paris rhizome according to right, it is characterized in that:
(a), the selection of propagating materials: get the rhizome of perennial band bud of the long connective Paris polyphylla of paris plant of wild or artificial planting, as the propagating materials of inducing clumping bud;
(b), the processing method of propagating materials: remove the soil on the rhizome, but can not hinder bud, will keep when the overground part plant is arranged, between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections with scraper behind the bud again, get a section of the band bud and make propagating materials;
(c), the processing time of propagating materials: the processing time is in 3～mid-August;
(d), the induced bundle environment and the management of sprouting: the propagating materials after the processing will in time be planted into the field and be chosen the Seedlings nursery of fertile sand, sandy loam or the Seedlings nursery of forming with vermiculite matrix; The Seedlings nursery environmental requirement shelters from heat or light, ventilates, cools, moistening; Induced environment requires to shelter from heat or light, ventilates, cools, moistening;
(e), the grow thickly transplantation technique of bud seedling:
Transplanting period: segment was handled back 3 years;
Transplanting time: the October～December of overground part plant jaundice;
Transplanting method: should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged, and should not be chosen in alkaline soil or the clay and cultivate; After choosing the plot, soil is ploughed deeply, in conjunction with apply base manure wholely autumn and winter; Shallow plowing before the plantation harrow brokenly, flattens bedding;
Transplant in the land for growing field crops by distance between rows and hills 20～25cm during transplanting, the bud head makes progress when planting, and root is unfolded in ditch, and the thick 4～5cm of earthing requires with digging with planting.
3, want the bud propagation method of growing thickly of 1 or 2 described long connective rhizoma paris rhizomes according to right, it is characterized in that:
(a), the selection of propagating materials: get the rhizome of perennial band bud of the long connective Paris polyphylla of paris plant of wild or artificial planting, as the propagating materials of inducing clumping bud;
(b), the processing method of propagating materials: remove the soil on the rhizome, but can not hinder bud, when being arranged, the overground part plant to keep, between the 3rd stem trace and the 5th stem trace rhizome is being cut into two sections with scraper behind the bud again, get one section of the band bud and make propagating materials, dip in fresh ash at the propagating materials wound after the segment;
(c), the processing time of propagating materials: the optimization process time is in 3～mid-August;
(d), the induced bundle environment and the management of sprouting: the propagating materials after the processing will in time be planted into the fertile sand in field, sandy loam or the Seedlings nursery formed with vermiculite matrix; The Seedlings nursery environmental requirement shelters from heat or light, ventilates, cools, moistening; Induced environment requires to shelter from heat or light, ventilates, cools, moistening; The field nursery stage is wanted diligent weeding, and carries out ditch cleaning row stain, drought-resistant moisture-preserving work, and will use sufficient the fertilizer of surviving the winter winter;
(e), the grow thickly transplantation technique of bud seedling:
Transplanting period: segment was handled back 3 years;
Transplanting time: October of portion plant jaundice on the ground;
Transplanting method: should be regional more than height above sea level 800m, select the humus content height, soil is more moistening, and the soil is more smooth, or the sand loam plot cultivation of concealment condition is arranged, and should not be chosen in alkaline soil or the clay and cultivate; After choosing the plot, soil is ploughed deeply 20～25cm, apply decomposed dung, compost 37 500～45 000kg or the cake-shaped rape seed dregs 1500kg that becomes thoroughly decomposed, plough under and make base manure in the soil in conjunction with whole ground per hectare autumn and winter; Shallow plowing before the plantation harrow brokenly, flattens bedding, the wide 1.2～1.5m of furrow, and the furrow face is turtleback shape, furrow furrow width 30cm, furrow ditch depth 10cm;
Transplant in the land for growing field crops by distance between rows and hills 20～25cm during transplanting, if plant the back antecedent soil moisture, should irrigate normal root water, in order to surviving, the bud head makes progress when planting, and root is unfolded in ditch, and the thick 4～5cm of earthing requires with digging with planting.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100524651A CN100527941C (en) | 2007-06-14 | 2007-06-14 | Paris polyphylla var. pseudothibetica rhizome multiple shoot asexual reproduction method |
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| CNB2007100524651A CN100527941C (en) | 2007-06-14 | 2007-06-14 | Paris polyphylla var. pseudothibetica rhizome multiple shoot asexual reproduction method |
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| CN100527941C CN100527941C (en) | 2009-08-19 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785384A (en) * | 2010-04-02 | 2010-07-28 | 安顺市维权种植有限公司 | Breeding method of paris polyphylla |
| CN102498880A (en) * | 2011-11-04 | 2012-06-20 | 云南农业大学 | Cutting seedling raising method of rodgersflower rhizome stem tubers |
| CN102870564A (en) * | 2012-09-26 | 2013-01-16 | 四川农业大学 | Fast efficient propagation method of Paris polyphylla |
| CN103548683A (en) * | 2013-10-31 | 2014-02-05 | 成都大学 | Embryoid induction method taking polyphylla bud axis as explant |
| CN103819236A (en) * | 2014-03-06 | 2014-05-28 | 西双版纳天源林石斛种植有限公司 | Fermentation substrate as well as preparation method and application thereof |
| CN104584842A (en) * | 2015-02-02 | 2015-05-06 | 红河山鼎生物资源开发有限公司 | Multi-head and multi-bud yield-increasing cultivation method for Paris polyphylla |
| CN104904474A (en) * | 2015-06-07 | 2015-09-16 | 湖北济世药业有限责任公司 | Paris polyphylla asexual reproduction method |
| CN105123253A (en) * | 2015-10-15 | 2015-12-09 | 王婧 | Seedling method for paris polyphylla |
| CN105284568A (en) * | 2015-11-02 | 2016-02-03 | 易门元源林农产品开发有限公司 | Floating seedling-raising method for amyloid Paris polyphylla var. yunnanensis rhizomes |
| CN106342598A (en) * | 2016-08-22 | 2017-01-25 | 兰坪易慧农业开发有限公司 | Planting method promoting growth of paris polyphylla |
| CN112913608A (en) * | 2021-01-21 | 2021-06-08 | 毕节市中药研究所 | Paris polyphylla wild-imitating cultivation method and Paris polyphylla wild-imitating cultivation equipment |
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2007
- 2007-06-14 CN CNB2007100524651A patent/CN100527941C/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785384A (en) * | 2010-04-02 | 2010-07-28 | 安顺市维权种植有限公司 | Breeding method of paris polyphylla |
| CN102498880A (en) * | 2011-11-04 | 2012-06-20 | 云南农业大学 | Cutting seedling raising method of rodgersflower rhizome stem tubers |
| CN102870564A (en) * | 2012-09-26 | 2013-01-16 | 四川农业大学 | Fast efficient propagation method of Paris polyphylla |
| CN102870564B (en) * | 2012-09-26 | 2014-03-12 | 四川农业大学 | Fast efficient propagation method of Paris polyphylla |
| CN103548683A (en) * | 2013-10-31 | 2014-02-05 | 成都大学 | Embryoid induction method taking polyphylla bud axis as explant |
| CN103819236B (en) * | 2014-03-06 | 2016-04-06 | 西双版纳天源林石斛种植有限公司 | Fermented substrate and its production and use |
| CN103819236A (en) * | 2014-03-06 | 2014-05-28 | 西双版纳天源林石斛种植有限公司 | Fermentation substrate as well as preparation method and application thereof |
| CN104584842A (en) * | 2015-02-02 | 2015-05-06 | 红河山鼎生物资源开发有限公司 | Multi-head and multi-bud yield-increasing cultivation method for Paris polyphylla |
| CN104904474A (en) * | 2015-06-07 | 2015-09-16 | 湖北济世药业有限责任公司 | Paris polyphylla asexual reproduction method |
| CN104904474B (en) * | 2015-06-07 | 2017-06-30 | 湖北济世药业有限责任公司 | A kind of asexual reproduction method of Paris polyphylla |
| CN105123253A (en) * | 2015-10-15 | 2015-12-09 | 王婧 | Seedling method for paris polyphylla |
| CN105123253B (en) * | 2015-10-15 | 2017-10-27 | 向君民 | A kind of method for culturing seedlings of paris polyphylla |
| CN105284568A (en) * | 2015-11-02 | 2016-02-03 | 易门元源林农产品开发有限公司 | Floating seedling-raising method for amyloid Paris polyphylla var. yunnanensis rhizomes |
| CN106342598A (en) * | 2016-08-22 | 2017-01-25 | 兰坪易慧农业开发有限公司 | Planting method promoting growth of paris polyphylla |
| CN112913608A (en) * | 2021-01-21 | 2021-06-08 | 毕节市中药研究所 | Paris polyphylla wild-imitating cultivation method and Paris polyphylla wild-imitating cultivation equipment |
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| CN100527941C (en) | 2009-08-19 |
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