CN101036447B - Seawater domestication and indoor high-density culture method of moina mongolica daday - Google Patents
Seawater domestication and indoor high-density culture method of moina mongolica daday Download PDFInfo
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- CN101036447B CN101036447B CN200710011131XA CN200710011131A CN101036447B CN 101036447 B CN101036447 B CN 101036447B CN 200710011131X A CN200710011131X A CN 200710011131XA CN 200710011131 A CN200710011131 A CN 200710011131A CN 101036447 B CN101036447 B CN 101036447B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention disclose a seawater domestication method for Moina mongolica Daday. The following is the concrete steps: sampling Moina mongolica Daday and original water in container, sampling density being less than 100/l, culturing Moina mongolica Daday in original water, adding filtered seawater each day to promote salinity 1-2 to achieve 30-35, illumination intensity being 5000-7000lx, photoperiod L:D=12h:12h, temperature being 25-28 degree, feeding each day of chlorella (Two million/ml) cultured by seawater until the Moina mongolica Daday growing normally and propagating.to achieve 2000-5000/l, taking the Moina mongolica Daday to micro flow water cultivation pool, using chlorella and yeast as continuous feeding feedstuff, illumination intensity being 5000-7000lx, photoperiod L:D=12h:12h, oxygen dissolution 6-8mg/l, water pH being 7-8. When the Moina mongolica Daday density is 15000-20000/L, harvesting is carried out.
Description
Technical field:
The present invention relates to a kind of salt solution cladocera---the seawater acclimation and the indoor high-density culture method of moina mongolica daday.
Background technology:
Moina mongolica daday (Moina mongolica Daday) is under the jurisdiction of Crustachia Branchiopoda peg graft, is the unique salt solution Moina that gains public acceptance of the Old World.This Magna has that adaptive faculty is strong, breeding is fast, is of high nutritive value, and contains advantages such as a large amount of fish essential amino acids and highly unsaturated fatty acid.Practice result shows; fish (as the freshwater fish village) and river crab growth that the application moina mongolica daday is cooked the bait nursing are fast; the survival rate height; on to the nutritive value of seawater fish even be higher than the halogen worm; therefore; moina mongolica daday is agreeable to the taste living bait Object of Development of the very big marine fish shrimps of a kind of potentiality and novel sea environment protection and monitoring animal; especially current seafood fish is grown seedlings and is produced relaying and need a large amount of artemia naupliis with wheel animalcule afterwards as open-mouthed bait; and the artemia cysts resource-constrained; and (50～600,000 yuan per ton) cost an arm and a leg; therefore partly or entirely substitute the halogen worm with moina mongolica daday; both can save artemia resource; can improve survival rate of seedling again, reduce the marine fish seedling cost, have very big society and economic implications.
At present, the moina mongolica daday Magna kind of research and application all is to pick up from the nitre pond of Yuncheng Prefecture, Shanxi Province (Jin Nan) nineteen eighty-two in the research and production at home, be able to extensive use in south as Fujian, Guangdong, Shanghai etc., but because the preference temperature higher (25 ℃) of its cultivation is not also carried out highdensity a large amount of cultivation and utilization at indoor seawater (salinity 30～35) to moina mongolica daday at northern marine fish mating season (4～June) up to now.
Summary of the invention:
The present invention is in order to solve existing in prior technology the problems referred to above, provide a kind of can be at northern marine fish mating season (4～June), cultivate moina mongolica daday in a large number at indoor seawater, partly or entirely to substitute the halogen worm, improve the seawater acclimation and the indoor high-density culture method of the moina mongolica daday of survival rate of fish fry, reduction albacore seedling cost.
Technical solution of the present invention is: the seawater acclimation method of moina mongolica daday is characterized in that having the following steps:
A. gather landlocked (Jin Nan or Inner Mongol) salt lake product moina mongolica daday and former water to container, gather density less than 100/l;
B. moina mongolica daday is cultivated in former water, add filtering sea and every day salinity is raise 1～2, make salinity reach 30～35 gradually, intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, temperature keeps 25～28 ℃, and the bead Magna that cultivate with seawater every day throws something and feeds, feeding volume is 2,000,000/ml, breeds until the moina mongolica daday normal growth.
2. the indoor high-density culture method of a moina mongolica daday is characterized in that following steps:
A. to Cultivation container and breed pond sterilization;
B. cancel malicious filtering sea, the pH value of adjusting seawater is 7～8,25～30 ℃ of temperature;
The moina mongolica daday of the described seawater acclimation of the claim 1 of c. learning from else's experience goes into to be loaded with in the Cultivation container of the described seawater of b step, inoculum density is 500～1000/l, the bead Magna that cultivates with seawater throws something and feeds, and day feeding volume 200～5,000,000/ml are 2000～5000/l to cultivating Magna density;
D. the moina mongolica daday after the c step of learning from else's experience is cultivated is to the growing pond of little flowing water type, introducing a fine variety density is 100～500/l, contain the chlorella that density is 200～5,000,000/ml in the miniflow water that flows into, culture the middle and later periods and mend yeast in the miniflow water that flows into, yeast density is 20～150mg/l; Culture intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, 25～30 ℃ of water temperatures, dissolved oxygen amount 6～8mg/l, transferring pH is 7～8; When brachionus plicatilis occurs, be sprinkled into the formaldehyde of 15～20mg/L;
E. reach 15000～20000/l when cultivating Magna density, gather.
Described is to sterilize with liquor potassic permanganate or 1 with 300mg/l to the Cultivation container sterilization: after the 10HCl solution soaking has been scrubbed the Cultivation container 5min of cleaning, again with thimerosal flushing 2～3 times; Described is to scrub with thimerosal to rinse well with thimerosal after culturing the pond to the Cultivation container sterilization again.
Described b step is to get filtering sea, and 25～30 ℃ of temperature add 250ml 8% clorox sterilization at least 24 hours in every t seawater, add in the 35g sodium thiosulfate again and chlorine residue, and making the pH value is 8.
The yeast of throwing something and feeding of described d step be with yeast with 300 mesh sieve thin,tough silk rub with the hands broken after, add 1 liter of wiring solution-forming of water by per 10 gram yeast, throw something and feed after placing an evening.
Described e step is to dredge between band liquid in 2～3 days to gather 15～20%, mends new liquid again, or once all gathers.
Adjust pH is for to adjust with HCI in the described d step.
The present invention tames moina mongolica daday with seawater acclimation method, make it adapt to normal growth breeding in seawater, and cultivate in a large number with the seawater batch production at northern marine fish mating season (4～June) with indoor high-density culture method, its density can reach 15000～20000/L, partly or entirely to substitute the halogen worm, improve survival rate of fish fry, reduce the albacore seedling cost.
Embodiment:
Embodiment 1:
Seawater acclimation adopts following method:
A. gather inland brine lake (Jin Nan or Inner Mongol) product moina mongolica daday and the former water in salt lake to container, the density of moina mongolica daday as the need long-distance transport, needs frequent openable container mouth, to guarantee former water oxygen gas abundance less than 100/l in the container; The long-distance transport time surpasses 48 hours, the chlorella of need throwing something and feeding;
B. moina mongolica daday is cultivated in former water, dose filtering sea former salinity water is raise 1～2 every day, make salinity reach 30～35 gradually, intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, temperature keeps 25～28 ℃, (2,000,000/ml) throw something and feed of the bead Magna that cultivates with seawater every day, detect by an unaided eye and can breed by normal growth until moina mongolica daday, seawater acclimation is finished.
The indoor high-density of moina mongolica daday is cultivated the following method that adopts:
A. to Cultivation container or breed pond sterilization; Cultivation container can adopt general use large beaker, hatching cylinder etc., and enlarged culture can be used 50L white plastic bucket and capacity 1t left and right sides tank or cement pit, cultivates employing capacity 5～30t cement pit in a large number;
B. the using sea area for aquatic breeding water that the routine disinfection of learning from else's experience filters, the pH value of adjusting seawater is 7～8, and the optimum value of basicity is 3mmol/l, and it is 25～30 ℃ that electrical heating or illumination make temperature;
C. the moina mongolica daday of seawater acclimation of learning from else's experience is gone into Cultivation container and is carried out kind of level and enlarged culture, inoculum density is 500～1000/l, the bead Magna that cultivates with seawater throws something and feeds, day feeding volume increases according to the density of Magna, usually feeding volume is 200～5,000,000/ml, is 2000～5000/l until the density of the moina mongolica daday of being cultivated;
D. the moina mongolica daday after the c step of learning from else's experience is cultivated (promptly culture water and be the microfluidic state) to the growing pond of little flowing water type, introducing a fine variety density is 100～500/l, containing density in the miniflow water of inflow is the chlorella of 200～5,000,000/ml; Containing in the miniflow water of inflow of chlorella and mend yeast culturing the middle and later periods, can reduce aquaculture cost, the density of mending yeast is 20～150mg/l, and promptly chlorella and yeast adopt continuous input mode, and concrete injected volume is decided in this scope according to Magna density; Culture intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, 25～30 ℃ of water temperatures, can first discontinuity after the continuity oxygenation, make the dissolved oxygen amount of culturing water remain on 6～8mg/l, adjusting the pH value of culturing water with HCI is 7～8; Observe, monitor, when brachionus plicatilis occurs, be sprinkled into the formaldehyde of 15～20mg/L;
E. reach 15000～20000/l when cultivating Magna density, carry out disposable gathering.
Embodiment 2:
A. gather inland brine lake product moina mongolica daday and the former water in salt lake to container, the 100/l of density of moina mongolica daday in the container as the need long-distance transport, needs frequent openable container mouth, to guarantee former water oxygen gas abundance; The long-distance transport time surpasses 48 hours, the chlorella of need throwing something and feeding;
B. moina mongolica daday is cultivated in former water, dose filtering sea former salinity water is raise 1 every day, make salinity reach seawater salinity (30～35) gradually, intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, temperature keeps 28 ℃, (2,000,000/ml) throw something and feed of the bead Magna that cultivates with seawater every day, detect by an unaided eye until the breeding of moina mongolica daday normal growth, seawater acclimation is finished.
The indoor high-density of moina mongolica daday is cultivated the following method that adopts:
A. to Cultivation container or breed pond sterilization; Cultivation container can adopt general use large beaker, hatching cylinder etc., and enlarged culture can be used 50L white plastic bucket and capacity 1t left and right sides tank or cement pit, a large amount of capacity of using 5～30t cement pits of cultivating; To the Cultivation container sterilization is with 300mg/l sterilization with liquor potassic permanganate or 1: the 10HCl solution soaking has been scrubbed behind the Cultivation container 5min of cleaning again with thimerosal flushing 2～3 times; To the Cultivation container sterilization is to scrub with thimerosal to rinse well with thimerosal after culturing the pond again;
B. get filtering sea, it is 25～30 ℃ that electrical heating or illumination make temperature, adds 250ml 8% clorox sterilization at least 24 hours in every t seawater, adds in the 35g sodium thiosulfate again and chlorine residue, and making the pH value is 7～8, and the optimum value of basicity is 3mmol/l;
C. the moina mongolica daday of seawater acclimation of learning from else's experience is gone into Cultivation container and is carried out kind of level and enlarged culture, introducing a fine variety density is 500～1000/l, the bead Magna that cultivates with seawater throws something and feeds, and day feeding volume 200～5,000,000/ml are 2000～5000/l until the density of the moina mongolica daday of being cultivated;
D. the moina mongolica daday after the above-mentioned c step of learning from else's experience is cultivated (promptly culture water and be the microfluidic state) to the growing pond of little flowing water type, introducing a fine variety density is 300～500/l, containing density in the miniflow water of inflow is the chlorella of 200～5,000,000/ml; Containing in the miniflow water of inflow of chlorella and mend yeast culturing the middle and later periods, can reduce aquaculture cost, the density of mending yeast is 20～150mg/l, and promptly chlorella and yeast adopt continuous input mode, and concrete injected volume is decided in this scope according to Magna density; The yeast of throwing something and feeding be with yeast with 300 mesh sieve thin,tough silk rub with the hands broken after, add 1 liter of wiring solution-forming of water by per 10 gram yeast, throw something and feed after placing an evening;
Culture intensity of illumination 5000～7000lx, photoperiod L: D=12h: 12h, 25～30 ℃ of water temperatures, can first discontinuity after the continuity oxygenation, make the dissolved oxygen amount of culturing water remain on 6～8mg/l, adjusting the pH value of culturing water with HCI is 7～8; Observe, monitor, when brachionus plicatilis occurs, be sprinkled into the formaldehyde of 15～20mg/L;
E. reach 15000～20000/L when cultivating Magna density, gather, can be with in per 1～2 day to dredge between liquid and gather 15～20%, mend new liquid again.
Claims (7)
1. the seawater acclimation method of a moina mongolica daday is characterized in that following steps:
A. gather moina mongolica daday and former water to container, gather density less than 100/l;
B. moina mongolica daday is cultivated in former water, add filtering sea every day salinity is raise 1～2, make salinity reach 30～35, cultivate intensity of illumination 5000～70001x, photoperiod L:D=12h:12h, temperature keeps 25～28 ℃, and the bead Magna that cultivate with seawater every day throws something and feeds, feeding volume is 2,000,000/ml, breeds until the moina mongolica daday normal growth.
2. the indoor high-density culture method of a moina mongolica daday is characterized in that following steps:
A. to Cultivation container and breed pond sterilization;
B. cancel malicious filtering sea, the pH value of adjusting seawater is 7～8,25～30 ℃ of temperature;
The moina mongolica daday of the described seawater acclimation of the claim 1 of c. learning from else's experience is gone in the Cultivation container of splendid attire sterilised filtration seawater, inoculum density is 500～1000/l, the bead Magna that cultivates with seawater throws something and feeds, and day feeding volume 200～5,000,000/ml are 2000～5000/l to cultivating Magna density;
D. the moina mongolica daday after the c step of learning from else's experience is cultivated is to the growing pond of little flowing water type, introducing a fine variety density is 100～500/l, contain the chlorella that density is 200～5,000,000/ml in the miniflow water that flows into, culture the middle and later periods and mend yeast in the miniflow water that flows into, yeast density is 20～150mg/l; Culture intensity of illumination 5000～70001x, photoperiod L:D=12h:12h, 25～30 ℃ of water temperatures, dissolved oxygen amount 6～8mg/l, transferring pH is 7～8; When brachionus plicatilis occurs, be sprinkled into the formaldehyde of 15～20mg/l;
E. reach 15000～20000/l when cultivating Magna density, gather.
3. the indoor high-density culture method of moina mongolica daday according to claim 2, it is characterized in that: described to the Cultivation container sterilization be scrubbed the Cultivation container 5min of cleaning with 300mg/l sterilization with liquor potassic permanganate or 1:10HCl solution soaking after, again with thimerosal flushing 2～3 times; Described is to scrub with thimerosal to rinse well with thimerosal after culturing the pond to culturing the pond sterilization again.
4. the indoor high-density culture method of moina mongolica daday according to claim 2, it is characterized in that: described b step is to get filtering sea, 25～30 ℃ of temperature, add 250ml8% clorox sterilization at least 24 hours in the seawater per ton, add in the 35g sodium thiosulfate again and chlorine residue, making the pH value is 7～8.
5. the indoor high-density culture method of moina mongolica daday according to claim 2 is characterized in that: the yeast of throwing something and feeding of described d step be with yeast with 300 mesh sieve thin,tough silk rub with the hands broken after, add 1 liter of wiring solution-forming of water by per 10 gram yeast, throw something and feed after placing an evening.
6. the indoor high-density culture method of moina mongolica daday according to claim 2 is characterized in that: described e step is once all to gather or dredged between band liquid in 1～2 day and gather 15～20%, mends new liquid again.
7. the indoor high-density culture method of moina mongolica daday according to claim 2, it is characterized in that: adjust pH is for to adjust with HCI in the described d step.
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| CN102138542B (en) * | 2011-02-28 | 2012-12-26 | 中山大学 | Method for reserving, conserving and culturing daphnia magna |
| CN102293183B (en) * | 2011-08-23 | 2013-04-24 | 浙江大学 | Ecological harvesting method for thallophyta daphnia |
| CN102972357A (en) * | 2012-12-20 | 2013-03-20 | 广西蓝浩海洋生物科技有限公司 | Intensive production method for Mongolian Moina |
| CN103190445B (en) * | 2013-04-17 | 2014-10-08 | 方艳玲 | Acid washing sterilization disinfectant and use method thereof |
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| CN104126550B (en) * | 2014-06-11 | 2016-06-29 | 广东汇美环保工程有限公司 | Tame large-scale method and method for repairing water ecology |
| CN105325373B (en) * | 2014-07-17 | 2019-01-08 | 蓝志娟 | A kind of method and method for repairing water ecology for taming daphnia |
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| CN104585140B (en) * | 2015-01-19 | 2016-12-07 | 蓝志娟 | A kind of water acclimation method and utilize water to dispatch from the factory the method that the water body of mud carries out restoration of the ecosystem to containing sewage treatment plant |
| CN104787893B (en) * | 2015-03-31 | 2017-01-04 | 上海太和水环境科技发展有限公司 | A kind of large-scale acclimation method and application thereof |
| CN105707011B (en) * | 2015-09-22 | 2019-01-25 | 陕西省水产研究所 | A kind of cladocera intensive manufacture breeding method |
| CN107667929B (en) * | 2017-09-29 | 2021-07-20 | 四川中科水务科技有限公司 | Cultivation method of deinsectization |
| CN109105308A (en) * | 2018-08-22 | 2019-01-01 | 榕江县陆氏鱼莊 | A kind of rice field fish culture field fry sterilizing equipment decontaminating apparatus |
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