CN101032230A - Stepped culture selecting method for breeding fugu obscurus - Google Patents

Stepped culture selecting method for breeding fugu obscurus Download PDF

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Publication number
CN101032230A
CN101032230A CNA2006100245235A CN200610024523A CN101032230A CN 101032230 A CN101032230 A CN 101032230A CN A2006100245235 A CNA2006100245235 A CN A2006100245235A CN 200610024523 A CN200610024523 A CN 200610024523A CN 101032230 A CN101032230 A CN 101032230A
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China
Prior art keywords
fugu obscurus
population
stage
phase
selection
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Pending
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CNA2006100245235A
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Chinese (zh)
Inventor
阳清发
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Shanghai Nengzheng Fishery Technology Development Co Ltd
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Shanghai Nengzheng Fishery Technology Development Co Ltd
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Priority to CNA2006100245235A priority Critical patent/CN101032230A/en
Publication of CN101032230A publication Critical patent/CN101032230A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The stage process of selectively breeding cultivated Fugu obscurus includes the following steps: 1. selecting parent fishes with high vitality, great weight and normal body characteristic from fishes with different source in adjustable selecting strength; and 2. optimizing the line in the key growth stages of Fugu obscurus including preoviposition stage, young fish stage, fish seed stage, commercial fish stage and mature stage. The present invention is superior in that the process can selectively breed new cultivated Fugu obscurus line with high yield, high quality and no toxicity, and that the technological process has wide application range, simple operation and fast effect.

Description

Culture the stage population selection of fugu obscurus
Technical field the present invention relates to culture the selection of fugu obscurus improved seeds, is exactly specifically to culture the stage population selection of fugu obscurus.
It mainly is to utilize the sea to catch river Puffer and part to propagate the river Puffer artificially and carry out the river Puffer and grow seedlings that the present China of background technology river Puffer is cultured, and is perhaps grown seedlings by external import river Puffer fertilized egg.Because extensive breed for many years and natural world lack the grow seedlings fugu obscurus wild stocks of usefulness of enough confessions, the fugu obscurus deterioration of variety is very serious, culture the fugu obscurus sex premature, resistance, growth rate descend, the fugu obscurus difference in size of commercial specification is big, therefore by some biological means, it is stable to optimize high yield and high quality, strong stress resistance, germplasm, even nontoxic fugu obscurus kind is that China's river Puffer is cultured necessary.
The patent that relates to fugu obscurus both at home and abroad has only artificial propagation CN98111600.0 and control poison to culture two patents of CN0213814.5, so far do not see the correlative study report of river Puffer breed breeding, more do not see the patent and the report of the stage population seed selection of fugu obscurus, and domestic special population of nontoxic fugu obscurus and the selection that does not still have specific adaptation in China's breeding environment and aquaculture model.
Summary of the invention the object of the present invention is to provide the stage population selection of a kind of cultivated asepsis fugu obscurus of stable high yield high-quality.
To achieve these goals, the technical scheme that the present invention takes is: culture the parent population that 1. the stage population selection of fugu obscurus selects separate sources for use, 4~8 of colonies that mainly comprise the different algebraically of directly propagating artificially from domestic different regions, that each colony therefrom optimizes is energetic, physical trait is normal, speckle is clear, body weight is greater than the parent population of average weight 15~20%, culture respectively as alternative parent population, selection intensity is 10~20%, and selection intensity can also be adjusted accordingly according to the research needs; 2. the preoviposition period of the critical period of fugu obscurus growth, alevin stage, postlarva phase, fingerling phase, marketable fish phase and the prematuration period five stages be the seed selection critical period, carry out continuously preferably through triennium, select the nontoxic population quick, strong stress resistance that grows.
Be meant preferably the preoviposition period of described the parent population of separate sources before laying eggs supported respectively in difference and produce in advance in the pond that every pond 100-200 as a cultured population, establishes 4-8 cultured population to parent population altogether.
Preferably be meant described alevin stage after fugu obscurus is laid eggs hatching, preferably energetic from prelarva, phototaxis is good, be distributed in water body prelarva at the middle and upper levels as initial breeding population, the prelarva that optimizes accounts for and hatches 50% of prelarva, selected prelarva moves in the nursery pond and supports to salable black prelarva seedling, transfers to Tu Chizhong and carries out the postlarva cultivation.
The described postlarva phase preferably is meant at fugu obscurus postlarva phase (3-5 centimetre), the postlarva of each colony is selected wherein 4-5 centimetre big individual fugu obscurus postlarva culture, and eliminates too small individuality, and mortality is 70-90%.
The described fingerling phase preferably is meant at fugu obscurus fingerling phase (body weight 40-150 gram), in the fingerling of each colony, select big individual fugu obscurus fingerling (body weight 100-150 gram) to culture, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 50-60%.
The described marketable fish phase preferably is meant at fugu obscurus marketable fish phase (body weight 300-600 gram), in the marketable fish of each colony, select big individual fugu obscurus marketable fish (body weight 400-600 gram) to culture, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 70-80%.
Preferably be meant the prematuration period of described prematuration period of fugu obscurus (body weight 500-800 gram), each colony the prematuration period select big individual fugu obscurus in the fish prematuration period fish (body weight 700-800 gram) culture, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 70-80%.
The present invention has following advantage: by to the many stage population seed selections of generation of fugu obscurus, it is stable to optimize high yield and high quality, strong stress resistance, germplasm, and the fugu obscurus new population particularly selects growth fast, the nontoxic population of strong stress resistance; This technology is applicable to the nursery of any scale, and operation is simple, instant effect, and the strain of selecting is to the adaptability and the tolerance height of local environment, and growth is fast, high efficiency.
Concrete embodiment
(1) select for use 4-8 population of separate sources to be total to 5000-10000 at first to parent population, that each colony therefrom optimizes is energetic, physical trait is normal, body weight is right greater than the parent population 500-1000 of average weight 15~20%, culture respectively as alternative parent population, selection intensity is 10~20%, selection intensity can also be adjusted accordingly according to the research needs, and the parent population of separate sources is all cultured separately, does not mix mutually with the parent population of other populations.
(2) preoviposition period of the critical period of fugu obscurus growth, alevin stage, postlarva phase, fingerling phase, marketable fish phase and the prematuration period five stages be the seed selection critical period, preferred continuously through triennium, select growth fast, the nontoxic kind of strong stress resistance.
1. the preoviposition period: the parent population of separate sources is supported separately respectively in difference and produces in advance in the pond before will laying eggs, and every pond 100-200 as a cultured population, establishes 4-8 cultured population to parent population altogether.
2. alevin stage: after fugu obscurus is laid eggs hatching, preferably energetic from prelarva, phototaxis is good, water body prelarva at the middle and upper levels is as initial breeding population, the prelarva that optimizes accounts for and hatches 50% of prelarva, selected prelarva moves in the fry pond and supports to salable black prelarva seedling (the long 0.7-1.0 of body centimetre), transfers to Tu Chizhong and carries out the postlarva cultivation.
3. postlarva phase: at fugu obscurus postlarva phase (the long 3-5 of body centimetre), in the postlarva of each colony, select big individual fugu obscurus postlarva (the long 4-5 of body centimetre) to culture, superseded too small individuality, mortality is 70-80%.
4. fingerling phase: at fugu obscurus fingerling phase (body weight 40-150 gram), in the fingerling of each colony, select big individual fugu obscurus fingerling (body weight 100-150 gram) to culture, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 50-60%.
5. marketable fish phase: at fugu obscurus marketable fish phase (body weight 300-600 gram), in the marketable fish of each colony, select big individual fugu obscurus marketable fish (body weight 400-600 gram) to culture, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 70-80%.
6. the prematuration period: the prematuration period of fugu obscurus (body weight 500-800 gram), each colony the prematuration period select big individual fugu obscurus in the fish prematuration period fish (body weight 700-800 gram) 500-1000 to culturing, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot (ovary 2-3 phase) not at random remains, and mortality is 70-80%.
This 500-1000 to fugu obscurus as standby parent population, accelerating, grow seedlings, proceed seed selection, preferred 100-200 continues respectively to raise to the parent population of parent population as stage population seed selection from each population, female milter from same breed pond should be separated when growing seedlings, culture the male raun mating in ponds with other, to avoid the inbreeding of parent population, this is the key of colony's seed selection, and other parent population can be used for general growing seedlings and produces or sell.
The present invention is applicable to the stage population seed selection of Puffer kind river, the different river Puffer that Fugu belongs to, should determine the seed selection standard according to the breed result in variety classes and per generation during concrete the application, what of used parent population quantity the population scale that wherein is used for seed selection be, can do suitably to adjust according to the scale of breed and nursery.

Claims (7)

1. culture the stage population selection of fugu obscurus, it is characterized in that:
1) selects the parent population of separate sources for use, directly the colony of the different algebraically of propagating artificially from domestic different regions is 4~8, that each colony therefrom optimizes is energetic, physical trait is normal, body weight is greater than the parent population of average weight 15~20%, culture respectively as alternative parent population, selection intensity is 10~20%, and selection intensity can also be adjusted accordingly according to the research needs;
2) preoviposition period of the critical period of fugu obscurus growth, alevin stage, postlarva phase, fingerling phase, marketable fish phase and the prematuration period five stages be the seed selection critical period, carry out continuously preferably through triennium, select growth fast, strong stress resistance, the nontoxic clearly fugu obscurus new population of speckle.
2. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: preferably be meant the preoviposition period of described the parent population of separate sources before laying eggs supported respectively and produce in the pond in advance in difference, every pond 100-200 as a cultured population, establishes 4-8 cultured population to parent population altogether.
3. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: preferably be meant described alevin stage after fugu obscurus is laid eggs hatching, preferably energetic from prelarva, phototaxis is good, water body prelarva at the middle and upper levels is as initial breeding population, the prelarva that optimizes accounts for and hatches 50% of prelarva, and selected prelarva moves in the fry pond and supports to salable black prelarva seedling, transfers to Tu Chizhong and carries out the postlarva cultivation.
4. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: the described postlarva phase preferably was meant in the fugu obscurus postlarva phase (3-5 centimetre) selects wherein 4-5 centimetre big individual fugu obscurus postlarva to culture the postlarva of each colony, eliminate too small individuality, mortality is 70-80%.
5. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: the described fingerling phase preferably was meant in the fugu obscurus fingerling phase selects wherein the big individual fugu obscurus fingerling of 100-150 gram to culture the fingerling 40-150 of each colony gram, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 50-60%.
6. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: the described marketable fish phase preferably was meant in the fugu obscurus marketable fish phase selects wherein the big individual fugu obscurus marketable fish of 400-600 gram to culture the marketable fish 300-600 of each colony gram, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 70-80%.
7. according to the stage population selection of the described breed fugu obscurus of claim 1, it is characterized in that: be meant preferably the prematuration period of described that the prematuration period of fugu obscurus fish is cultured the prematuration period that the prematuration period fish 500-800 gram of each colony being selected wherein the big individual fugu obscurus of 700-800 gram, eliminate too small individuality, the clear-cut texture individuality of selecting to connect health both sides blackspot not at random remains, and mortality is 70-80%.
CNA2006100245235A 2006-03-09 2006-03-09 Stepped culture selecting method for breeding fugu obscurus Pending CN101032230A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940185A (en) * 2010-09-28 2011-01-12 上海能正渔业科技开发有限公司 Staged population selective breeding method of Takifugu obscurus non-toxic pedigree
CN102273416A (en) * 2011-06-02 2011-12-14 中国水产科学研究院淡水渔业研究中心 Method for promoting synchronous development of sexual glands of takifugu obscurus male and female parent fishes
CN102524135A (en) * 2012-02-17 2012-07-04 中国水产科学研究院淡水渔业研究中心 Pool mixed culture method for takifugu obscurus offspring seeds and paracanthobrama guichenoti offspring seeds
CN104686423A (en) * 2015-03-23 2015-06-10 江苏中洋集团股份有限公司 Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus
CN104719208A (en) * 2015-03-23 2015-06-24 江苏中洋集团股份有限公司 Cultivation method of non-toxic takifugu obscurus abe fine breed
CN110269009A (en) * 2019-07-19 2019-09-24 重庆两江生态渔业发展有限公司 Bighead selection for reservoir cultivation
CN113615605A (en) * 2020-08-11 2021-11-09 中国水产科学研究院南海水产研究所 High-strength selecting and retaining construction method for high-quality parent population of eleutheronema tetradactylum

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940185A (en) * 2010-09-28 2011-01-12 上海能正渔业科技开发有限公司 Staged population selective breeding method of Takifugu obscurus non-toxic pedigree
CN102273416A (en) * 2011-06-02 2011-12-14 中国水产科学研究院淡水渔业研究中心 Method for promoting synchronous development of sexual glands of takifugu obscurus male and female parent fishes
CN102524135A (en) * 2012-02-17 2012-07-04 中国水产科学研究院淡水渔业研究中心 Pool mixed culture method for takifugu obscurus offspring seeds and paracanthobrama guichenoti offspring seeds
CN102524135B (en) * 2012-02-17 2013-10-09 中国水产科学研究院淡水渔业研究中心 Pool mixed culture method for takifugu obscurus offspring seeds and paracanthobrama guichenoti offspring seeds
CN104686423A (en) * 2015-03-23 2015-06-10 江苏中洋集团股份有限公司 Colony-family-molecule comprehensive genetic breeding method for takifugu obscurus
CN104719208A (en) * 2015-03-23 2015-06-24 江苏中洋集团股份有限公司 Cultivation method of non-toxic takifugu obscurus abe fine breed
CN104686423B (en) * 2015-03-23 2016-09-14 江苏中洋集团股份有限公司 A kind of method of Puffer fish colony family molecular association genetic breeding
CN110269009A (en) * 2019-07-19 2019-09-24 重庆两江生态渔业发展有限公司 Bighead selection for reservoir cultivation
CN113615605A (en) * 2020-08-11 2021-11-09 中国水产科学研究院南海水产研究所 High-strength selecting and retaining construction method for high-quality parent population of eleutheronema tetradactylum

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Open date: 20070912