CN100549169C - 酵母中来自Tritirachium album的重组蛋白酶K的表达 - Google Patents

酵母中来自Tritirachium album的重组蛋白酶K的表达 Download PDF

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Publication number
CN100549169C
CN100549169C CNB028047745A CN02804774A CN100549169C CN 100549169 C CN100549169 C CN 100549169C CN B028047745 A CNB028047745 A CN B028047745A CN 02804774 A CN02804774 A CN 02804774A CN 100549169 C CN100549169 C CN 100549169C
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dna
proteinase
parent
host cell
proenzyme
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Chinese (zh)
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CN1592785A (zh
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R·米勒
J·-P·塔尔霍菲
F·盖佩尔
S·格拉泽尔
W·赫尔克
H·舍恩
T·柯施鲍姆
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Meat, Egg Or Seafood Products (AREA)
CNB028047745A 2001-02-09 2002-02-05 酵母中来自Tritirachium album的重组蛋白酶K的表达 Expired - Lifetime CN100549169C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10105911A DE10105911A1 (de) 2001-02-09 2001-02-09 Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE10105911.6 2001-02-09

Publications (2)

Publication Number Publication Date
CN1592785A CN1592785A (zh) 2005-03-09
CN100549169C true CN100549169C (zh) 2009-10-14

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CNB028047745A Expired - Lifetime CN100549169C (zh) 2001-02-09 2002-02-05 酵母中来自Tritirachium album的重组蛋白酶K的表达

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US (1) US7368274B2 (https=)
EP (1) EP1360283B1 (https=)
JP (1) JP2004517643A (https=)
CN (1) CN100549169C (https=)
AT (1) ATE353960T1 (https=)
CA (1) CA2435606C (https=)
CZ (1) CZ304969B6 (https=)
DE (2) DE10105911A1 (https=)
DK (1) DK1360283T3 (https=)
ES (1) ES2280516T3 (https=)
WO (1) WO2002064760A2 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153666B2 (en) 2003-07-17 2006-12-26 General Atomics Methods and compositions for determination of glycated proteins
ATE514774T1 (de) * 2004-04-02 2011-07-15 Hoffmann La Roche Hoch aufgereinigte proteinase k
US7855079B2 (en) 2006-07-25 2010-12-21 General Atomics Methods for assaying percentage of glycated hemoglobin
US7943385B2 (en) 2006-07-25 2011-05-17 General Atomics Methods for assaying percentage of glycated hemoglobin
EP2283149A1 (en) * 2008-05-13 2011-02-16 General Atomics Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
CN102839165B (zh) * 2012-09-26 2014-12-10 金普诺安生物科技（苏州）有限公司 基因突变型重组蛋白酶k及其工业化生产方法
CN104059900A (zh) * 2013-03-19 2014-09-24 中国科学院天津工业生物技术研究所 一种蛋白酶k及其应用
CN105087529A (zh) * 2014-05-07 2015-11-25 天津旭晨科技有限公司 基因工程改造蛋白酶k及其生产方法
CN105193640B (zh) * 2014-06-24 2018-10-12 金普诺安蛋白质工程技术（北京）有限公司 蛋白酶k在皮肤保健和化妆品领域中的应用
CN108450952A (zh) * 2018-02-08 2018-08-28 深圳市唯姿贸易有限公司 一种细胞修复口服液
CN110106160B (zh) * 2019-05-29 2021-07-20 中国农业科学院北京畜牧兽医研究所 催化活性提高的碱性蛋白酶突变体prok-g及其编码基因和应用
CN112048518B (zh) * 2020-08-22 2022-06-24 江西农业大学 一种玉米醇溶蛋白降解酶的制备方法及其应用
CN114181925B (zh) * 2020-09-14 2025-01-17 武汉禾元生物科技股份有限公司 一种重组蛋白酶k工业化纯化及冻干方法
CN112592931A (zh) * 2020-12-31 2021-04-02 安徽丰原发酵技术工程研究有限公司 一种生产重组蛋白酶k的方法
CN113337492B (zh) * 2021-06-02 2023-04-07 武汉瀚海新酶生物科技有限公司 一种蛋白酶k耐热突变体
CN113481225A (zh) * 2021-07-23 2021-10-08 武汉瀚海新酶生物科技有限公司 一种蛋白酶k高表达工程菌株的构建及应用
PL244825B1 (pl) 2021-11-26 2024-03-11 Blirt Spolka Akcyjna Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K
CN115011583A (zh) * 2021-11-26 2022-09-06 河南合智医药科技有限公司 高表达高比活蛋白酶k突变体序列、毕赤酵母表达质粒的构建及菌株筛选和纯化方法
CN115851680A (zh) * 2022-11-22 2023-03-28 山东恒鲁生物科技有限公司 一种菌的培养物及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3537708A1 (de) 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
JP2731407B2 (ja) * 1987-04-03 1998-03-25 アムジエン・インコーポレーテツド 新規なプロテアーゼ酵素
US5191063A (en) 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Clonging and expression of the gene encoding a novelproteinase from Tritirachium album limber.. SAMAL B等.ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY,Vol.379 . 1996 *
Purification and characterization of a soybean root nodulephosphatase expressed in Pichia pastoris.. PENHEITER ALAN等.Protein expression and purification,Vol.14 No.1. 1998 *

Also Published As

Publication number Publication date
CZ20032163A3 (cs) 2004-05-12
JP2004517643A (ja) 2004-06-17
CN1592785A (zh) 2005-03-09
CA2435606A1 (en) 2002-08-22
CA2435606C (en) 2011-07-19
ES2280516T3 (es) 2007-09-16
DE10105911A1 (de) 2002-08-14
WO2002064760A2 (de) 2002-08-22
CZ304969B6 (cs) 2015-02-18
DE50209478D1 (de) 2007-03-29
DK1360283T3 (da) 2007-06-11
EP1360283A2 (de) 2003-11-12
ATE353960T1 (de) 2007-03-15
EP1360283B1 (de) 2007-02-14
WO2002064760A3 (de) 2002-12-27
US20040166560A1 (en) 2004-08-26
US7368274B2 (en) 2008-05-06

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