CN100486441C - Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew - Google Patents
Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew Download PDFInfo
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- CN100486441C CN100486441C CNB2007100458946A CN200710045894A CN100486441C CN 100486441 C CN100486441 C CN 100486441C CN B2007100458946 A CNB2007100458946 A CN B2007100458946A CN 200710045894 A CN200710045894 A CN 200710045894A CN 100486441 C CN100486441 C CN 100486441C
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Abstract
The present invention relates to a method capable of utilizing yellow locust stem and leaf extract to prepare aqueous preparation for effectively controlling powdery mildew of cucumber. Said aqueous preparation composition includes (by wt%) 0.2-4% of yellow locust stem and leaf extract, and the rest is water. Besides, said invention also provides the preparation method of said aqueous preparation and its concrete operation steps.
Description
Technical field
That the present invention relates to is a kind of preparation method of technical field of pesticide, specifically is a kind of preparation method with water solution with locust stems and leaves extraction for controlling cucumber powdery mildew.
Background technology
Powdery mildew of cucumber is short, the popular strong leaf diseases of a kind of incubation period that is caused by Sphaerotheca fuliginea (Schlecht) Poll., generally can make cucumber underproduction 5-20%, is the key factor of restriction cucumber stable high yield.Produce at present and go up main this disease of chemical pesticide control that adopts, but found that powdery mildew has almost produced pesticide resistance simultaneously to 7 series bactericidal agents (benzimidazole class, EBIs, organic phosphates, hydroxy pyrimidine class, triazole type, the rare esters of gallic acid of methoxy propyl and phenoxyquinolines class), and resistance development speed is surprising; Simultaneously because long-term a large amount of chemical pesticide that uses makes adverse effects such as high residue and environmental pollution become increasingly conspicuous.The goal of the invention of this patent promptly utilizes the aqua of Nantural non-toxic plant locust stem-leaf extract preparation to control the generation of powdery mildew of cucumber.Be to reduce the use of chemical pesticide, produce green or organic vegetable provides technical basis.Locust tree (Robiniapseudoacacia Linnl) is a pulse family robinia deciduous tree, and plantation is all arranged all over the world, aboundresources, and biomass is big; Locust tree is widely used in aspects such as feed, greening, water and soil conservation, also is a kind of nectariferous plant preferably and construction material.Contain the various active composition in the locust tree, have antibiotic, diuresis, anti-inflammatory, spasmolysis, heat-clearing, phlegm-dispelling functions, some active components are used to improve the capillary microcirculation, the ancillary drug of treatment acute and chronic nephritis, uremic, cystitis and gynecological disease.Active component mainly is robinin, agglutinin, rutin and Quercetin and multiple flavonoids material etc. in the bibliographical information locust tree.
Through the prior art literature search is found, Chen Jiao etc. have reported that the locust tree extract is inhibited to the downy mildew of garpe bacterium in " research and development of natural products " (2002 05 phases) is entitled as the article of " 58 plant species extracts are to the bacteriostatic activity screening study of downy mildew of garpe bacterium ".But also be not used to prevent and treat the research report of powdery mildew of cucumber at present about the locust tree extract.Also find in the retrieval, Chinese patent publication number CN1788572, patent name " method for preparing control cucurbits powdery mildew aqua with Radix seu Caulis Parthenocissi tricuspidatae extract ", this patent is mentioned the ethanol extract with the humid euphorbia herb, through the extracting n-butyl alcohol rear defence melon powdery mildew of harnessing the Yellow River, but the humid euphorbia biomass is little in this method, and impurity is more in the bacteriostatic active ingredients.
Summary of the invention
The objective of the invention is at above shortcomings in the prior art, a kind of preparation method with water solution with locust stems and leaves extraction for controlling cucumber powdery mildew is provided, make it have higher preventive effect to powdery mildew of cucumber, this method has been removed impurity a large amount of in the extract, can be directly used in this disease of control, and have nuisanceless, pollution-free and do not have the characteristics of residual hazard, avoided the harm of chemical pesticide.
The present invention is achieved by the following technical solutions, and described control powdery mildew of cucumber aqua component weight percent content is: locust stems and leaves extraction 0.2-4%, and surplus is a water, may further comprise the steps:
(1) will locust stems and leaves be ground into powder after the oven dry, add 5-20 doubly to the ethanol of leaf of locust powder weight as extractant, dipping extracts under the room temperature, obtains the locust stems and leaves ethanol crude extract after the filtration.
(2) with the method for crude extract, it is concentrated into paste, gets locust stems and leaves medicinal extract with concentrating under reduced pressure.
(3) add the distilled water equate with the locust stems and leaves powder quality in the locust stems and leaves medicinal extract, place supersonic generator then, use isopyknic extracting n-butyl alcohol again the medicinal extract suspension, get lower floor's aqueous phase solution after the extraction be evaporated to dried, must the water extract.
(4) the water extract is dissolved in its quality 10-20 distilled water doubly, with the absorption of HZ841 macroporous absorbent resin, uses a large amount of distilled water wash-outs then again, collection water elution liquid is evaporated to dried, promptly gets locust stems and leaves extraction, promptly gets aqua with dissolved in distilled water.
In the step (1), described oven dry, its temperature is 40 ℃.
In the step (1), described pulverizing is meant with medicinal herb grinder to be ground into 10-50 purpose plant powder.
In the step (1), described ethanol, its volumetric concentration is more than 60%.
In the step (1), described dipping extracts, and is meant that the solidliquid mixture with locust stems and leaves powder and ethanol places under 25 ℃ of conditions, leaves standstill 24 hours after fully stirring, and extracts.
In the step (1), described dipping extracts, and its number of times is 2-4 time.
In the step (2), described concentrating under reduced pressure is meant solution is vacuumized-0.1 atmospheric pressure that reduces pressure, and places the rotary water bath below 60 ℃, and solution is concentrated.
In the step (3), described extraction, its number of times is 4-6 time.
In the step (4), described HZ841 macroporous absorbent resin, its consumption are more than 200 times of quality of water extract dry matter.
In the step (4), described absorption, its time is more than 24 hours.
The present invention compared with prior art has following advantage: the aqua that the present invention makes derives from locust tree stem leaves of plants extract, utilize its water-soluble characteristics, be mixed with aqua, so environmentally safe, to people, animal safety, be a kind of pollution-free plant source biological bactericide; Gained sterilization aqua of the present invention is that, aboundresources wide to distribute and the great locust stems and leaves of biomass are raw material, be easy to artificial cultivation, extraction process is simple, need not special instrument, the bacteriostatic active ingredients content that obtains is higher, solvent after reclaiming simultaneously also can reuse, and this aqua has fabulous preventive effect to powdery mildew of cucumber, can reach 89.0%.。
Embodiment
Below embodiments of the invention are elaborated: present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1:
Control powdery mildew of cucumber aqua weight percent content: locust stems and leaves extraction 0.2%, distilled water 99.8%.
Take by weighing locust stems and leaves powder (20 order particle) 100g, extract 2 times with 60% ethanol 500ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 9.0g; Utilize ultrasonic, dry matter is suspended with 100ml water, equal-volume extracting n-butyl alcohol 4 times, the water extract concentrates, and obtains dry matter 2.5g; With 50ml dissolved in distilled water dry matter, with 1000g macroporous absorbent resin absorption 24 hours, with the desorb of 1500ml distilled water, collect washing lotion be evaporated to dried, extract 2.2g, be mixed with concentration and be 0.2% aqua; Adopt the leaf disc method that powdery mildew of cucumber is carried out indoor pharmacodynamic test, the preventive effect of powdery mildew of cucumber is reached 89.0%.
Embodiment 2:
Control powdery mildew of cucumber aqua weight percent content: locust stems and leaves extraction 2%, distilled water 99%.
Take by weighing tomato stem-leaf powder (20 order particle) 100g, extract 3 times with 80% ethanol 1000ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 8.5g; Utilize ultrasonic, dry matter is suspended with 100ml water, equal-volume extracting n-butyl alcohol 5 times, the water extract concentrates, and obtains dry matter 2.2g; With 40ml dissolved in distilled water dry matter, with 750g macroporous absorbent resin absorption 24 hours, with the desorb of 1250ml distilled water, collect washing lotion be evaporated to dried, extract 2.0g, be mixed with concentration and be 2% aqua; Adopt indoor pot experiment that powdery mildew of cucumber is carried out indoor pharmacodynamic test, the preventive effect of powdery mildew of cucumber is reached 78.0%.
Embodiment 3:
Control powdery mildew of cucumber aqua weight percent content: locust stems and leaves extraction 4%, distilled water 96%.
Take by weighing tomato stem-leaf powder (20 order particle) 100g, extract 3 times with absolute ethyl alcohol 2000ml room temperature dipping, each 24 hours, concentrating under reduced pressure reclaimed solvent and gets dry matter 7.2g; Utilize ultrasonic, dry matter is suspended with 100ml water, equal-volume extracting n-butyl alcohol 5 times, the water extract concentrates, and obtains dry matter 2.0g; With 30ml dissolved in distilled water dry matter, with 500g macroporous absorbent resin absorption 24 hours, with the desorb of 1000ml distilled water, collect washing lotion be evaporated to dried, extract 1.7g, be mixed with concentration and be 4% aqua; Adopt field plot trial that powdery mildew of cucumber is carried out the test of pesticide effectiveness, the preventive effect of powdery mildew of cucumber is reached 65.0%.
Claims (1)
1, a kind of preparation method with water solution with locust stems and leaves extraction for controlling cucumber powdery mildew is characterized in that described control powdery mildew of cucumber aqua component weight percent content is: locust stems and leaves extraction 0.2-4%, and surplus is a distilled water, may further comprise the steps:
(1) locust stems and leaves is dried the back under 40 ℃ of temperature and be ground into 10-50 purpose plant powder with medicinal herb grinder, adding 5-20 is that 60%～100% ethanol is as extractant to the volumetric concentration of leaf of locust powder weight doubly, the solidliquid mixture of locust stems and leaves powder and ethanol is placed under 25 ℃ of conditions, after leaving standstill 24 hours after fully stirring, extract 2-4 time, obtain the locust stems and leaves ethanol crude extract after the filtration;
(2) crude extract is vacuumized-0.1 atmospheric pressure that reduces pressure, place the rotary water bath below 60 ℃, solution is concentrated, it is concentrated into paste, get locust stems and leaves medicinal extract;
(3) add the distilled water that equates with the locust stems and leaves powder quality in the locust stems and leaves medicinal extract, place supersonic generator that medicinal extract is suspended then, use isopyknic extracting n-butyl alcohol 4-6 time again, get lower floor's aqueous phase solution after the extraction be evaporated to dried, the water extract;
(4) the water extract is dissolved in its quality 10-20 distilled water doubly, be equivalent to 250 times～400 times of quality the HZ841 macroporous absorbent resin absorption 24 hours of water extract dry matter again with consumption, use a large amount of distilled water wash-outs then, collecting water elution liquid is evaporated to dried, promptly get locust stems and leaves extraction, promptly get described control powdery mildew of cucumber aqua with dissolved in distilled water.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100458946A CN100486441C (en) | 2007-09-13 | 2007-09-13 | Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew |
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| CNB2007100458946A CN100486441C (en) | 2007-09-13 | 2007-09-13 | Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101518267B (en) * | 2009-04-02 | 2013-06-19 | 上海交通大学 | Aqueous pesticide solution compounded by silver chain extract and tea saponin and method for preparing same |
| CN102197810A (en) * | 2011-04-01 | 2011-09-28 | 上海交通大学 | Pinitol water aqua for controlling crop powdery mildew and preparation method thereof |
| CN103004894A (en) * | 2012-12-04 | 2013-04-03 | 上海交通大学 | Biopesticide for preventing and treating powdery mildew of melons and fruits and preparation method of biopesticide |
| ES2607147B1 (en) * | 2015-09-29 | 2018-01-17 | Biopharma Research, S.A. | USE OF AN ACACIA VEGETABLE EXTRACT TO PROMOTE RADICULAR GROWTH AND ACTIVITY IN PLANTS |
| CN105746610B (en) * | 2016-03-23 | 2018-06-26 | 国际竹藤中心 | A kind of synergist of plant source aphicide |
| CN105638755B (en) * | 2016-03-23 | 2018-06-19 | 国际竹藤中心 | A kind of plant insecticide and preparation method thereof |
| CN108184913A (en) * | 2017-12-20 | 2018-06-22 | 上海农乐生物制品股份有限公司 | It is a kind of for botanical pesticide of cucumber and preparation method thereof |
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