CN103125780A - Compound Chinese herbal preparation for improving testudinate immunity - Google Patents
Compound Chinese herbal preparation for improving testudinate immunity Download PDFInfo
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Abstract
The invention discloses a compound Chinese herbal preparation for improving testudinate immunity, belonging to the technical field of feed additives. The compound Chinese herbal preparation is characterized by comprising the following substances by weight percent: 8-12% of paulownia fortunei, 8-15% of wild chrysanthemum, 8-15% of flos lonicerae, 8-15% of pilosa, 8-15% of herba taxilli, 8-15% of radix glycyrrhizae, 8-15% of rhizoma polygoni cuspidate and 8-15% of hawthorn. Chinese herbal medicines have effects of resisting corrosion, astringing, promoting tissue regeneration, detoxifying, stopping bleeding and eliminating swelling, so that the testudinate immunity is improved, the incidence rate of testudinate skin disease is reduced, and furthermore, the breeding survival rate of testudinate is increased. The raw materials of the preparation are all selected from the natural world, the preparation is safe to people and animals, generates no residue and belongs to a green additive, the breeding survival rate of testudinate is increased, and the preparation plays a technical support function of breeding pollution-free green aquatic products.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of Chinese herbal formula that improves testudinate immunity.
Background technology
The aquaculture model of present domestic testudinate is under the background of pursuing high yield, greenhouse cultivation or the cultivation of the outer pool are all take high density as main, in addition thin because of quality mind, the brand awareness of Terrapin raising production, and cause the product quality quality to mix, make testudinate as the vivid sustain damage of health food, cause the testudinate price to continue to fall, fanning economics seriously comes down.Improving the product quality of testudinate, strengthen its immunity function, reduce and prevent and treat the medicament administration of disease, both met the production requirement of organic farm products, is also the study hotspot of current aquaculture.Chinese herbal and crude drugs preparations not only has direct inhibitory or killing effect to pathogenic microorganism, also can by the anti-stress ability of transferring body, immunity and the defense function that improves body, strengthen its premunition, and be difficult for producing drug resistance with rear less residue, pathogen.The harm that testudinate is caused to reduce disease ensures the sound development of Terrapin raising industry.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of technical scheme that relates to the compatibility of several traditional Chinese medicine ingredients and can improve the compound preparation and preparation method thereof of testudinate immunity, said preparation has the testudinate of significantly improving immunity, reduces the characteristics that disease occurs.
The Chinese herbal formula of raising testudinate immunity of the present invention, its mass percent is composed as follows: fortune paulownia flower 8～12%, mother chrysanthemum 8～15%, honeysuckle 8～15%, beggar-ticks 8～15%, parasitic loranthus 8～15%, Radix Glycyrrhizae 8～15%, giant knotweed 8～15%, hawthorn 8～15%.
At first Chinese herbal formula preparation method of the present invention takes respectively various raw materials according to mass percent, then raw material is pulverized, and sieves, and mixing namely makes.
Chinese herbal formula of the present invention is as the application of terrapin feed addictive.
Chinese herbal formula of the present invention can also act on water disinfectant and use.
That Chinese herbal and crude drugs preparations of the present invention has is anticorrosion, the effect of convergence, myogenic, detoxifcation, hemostasis, detumescence.Respond well, produce effects is quick, lasting medicine, low to environmental toxicity, the difficult drug resistance that produces.And preparation technology is simple, easily realizes, uses safety, convenient, easy to utilize.
The specific embodiment
The fortune paulownia flower that the present invention uses is the flower of dicotyledon medicine goatweed paulownia or royal paulownia.Have wind-dispelling heat-dissipating, clearing heat and detoxicating, the effect of clearing liver and improving vision.Control the infection of the upper respiratory tract, Bronchopneumonia, acute tonsillitis, bacillary dysentery, chordapsus, acute conjunctivitis, mumps, furuncle etc.
Described mother chrysanthemum is the dry aerial parts of saururaceae plant houttuynia cordata.Have clearing heat and detoxicating; Removing pus and relieving carbuncle; The effects such as inducing diuresis for treating strangurtia.
Described honeysuckle is Caprifoliaceae woodbine honeysuckle and congener dry flower or the first flower of opening of band.The effects such as resisting pathogenic microbes, anti-inflammatory and analgesic, the function that strengthens the defense, antiendotoxin.
Described beggar-ticks is for being the dry herb of feverfew beggar-ticks.Between Xia Qiu, Bian receives grass, removes earth, dries.Have clearing heat and detoxicating, the hemostasis antidiarrheal, the effects such as eliminating stasis to subdue swelling.Be usually used in appendicitis, ephritis, cholecystitis, enteritis, bacillary dysentery, hepatitis, peritonitis, the infection of the upper respiratory tract, tonsillitis, laryngitis, venomous snake bite, traumatic injury, skin infection, the diseases such as child convulsion, infantile malnutrition due to digestive disturbances or intestinalparasites are scalded in amenorrhoea.
Described parasitic loranthus is the stem and branch with leaf of Loranthaceae evergreen dwarf shrub plant parasitic loranthus or mistletoe.Wines used as antirheumatic is arranged, liver and kidney benefiting, strengthening the bones and muscles, antiabortive effect.
Described giant knotweed is dry rhizome and the root (leaf also is used as medicine) of polygonaceae Polygonum herbaceos perennial giant knotweed, mainly contain the compositions such as anthraquinone analog compound, polydatin, water-soluble polysaccharide and tannin, nature and flavor little hardship that is slightly cold, return liver, courage, lung channel, expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum effect are arranged.
Described Radix Glycyrrhizae is the dry root and rhizome of glycyrrhizic legume, glycyrrhiza inflate bat or glycyrrhiza glabra.Function cures mainly clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc.Happiness sunlight is abundant, the low dry climate of long temperature at sunshine.Radix Glycyrrhizae is grown in arid, semiarid desert steppe, desert edge and loess hill area more.
Described hawthorn is food plant, stone fruit, and matter is hard, and pulp is thin, distinguishes the flavor of little sour and astringent.Machaka.The close life of branch has spinelet, and sprout has pubescence.The sprig puce, old branch taupe.Can prevention and cure of cardiovascular disease.
For a more clear understanding of the present invention and practical application effect, below the present invention is described in further detail by specific embodiment and correlation test, and these are only the present invention embodiment preferably, but are not limited to these embodiment.
Embodiment 1
Take respectively by mass percentage fortune paulownia flower 100g, mother chrysanthemum 150g, honeysuckle 100g, beggar-ticks 100g, parasitic loranthus 100g, Radix Glycyrrhizae 150g, giant knotweed 150g, hawthorn 150g; Mix or pulverize separately, then filtering, filtering fineness requires to be 90-120 order, loss on drying≤8.0%.
The percentage composition that relates in present specification unless otherwise indicated all refers to the quality percentage composition.
Embodiment 2
Take respectively by mass percentage fortune paulownia flower 10%, mother chrysanthemum 10%, honeysuckle 15%, beggar-ticks 10%, parasitic loranthus 15%, Radix Glycyrrhizae 15%, giant knotweed 10%, hawthorn 15%.Fineness requirement is the 90-120 order, loss on drying≤10.0%.Get required Chinese herbal and crude drugs preparations.
Embodiment 3
Mass percent takes respectively fortune paulownia flower 15%, mother chrysanthemum 10%, honeysuckle 15%, beggar-ticks 15%, parasitic loranthus 10%, Radix Glycyrrhizae 15%, giant knotweed 10%, hawthorn 10%.Fineness requirement is the 90-120 order, loss on drying≤10.0%.Get required Chinese herbal and crude drugs preparations.
Those skilled in the art can be prepared the expansion of scale or dwindle according to the record of embodiment.
The use of said herbal medicine preparation can be taken orally, for example mixes to raise as additive and throws something and feeds.For example add Chinese herbal and crude drugs preparations 5-10 gram of the present invention in every Kg feed, be used in conjunction 2-4 days.
If as external application, can carry out water body disinfection, using method is 1-2 gram/M
3Water is used in conjunction 2 days.
The salivary lysozyme that draws below in conjunction with the Chinese herbal and crude drugs preparations feeding experiment is described further the result of use of Chinese herbal and crude drugs preparations of the present invention.
1.1 test period and place
Culture experiment carries out in the intelligent breeding soft-shell turtle of Huzhou City of Zhejiang Province field.The test site is the cement brick xoncrete structure, 48 meters of spans, 54 meters of length.The up and down was two-layer in indoor minute, and separate tanks is 21M
2, having 36 ponds, the water body height is controlled in 0.5 meter, and water temperature is controlled at 28-31 ℃ throughout the year, and density is 23/m
2
Chinese herbal and crude drugs preparations prepares according to the record of embodiment 1, and licorice polysaccharide is provided by the Wuhan legendary god of farming, and the feed of throwing something and feeding is that the children that Huzhou Longma feed company produces becomes trionyx sinensis feed, throws something and feeds every day 3 times.
Experiment is divided into 5 groups: the I group is feeding Chinese herbal and crude drugs preparations (blank) not, the every kilogram of feed of II group is added the Chinese herbal and crude drugs preparations (7.5g/kg) of 7.5g embodiment 1, the III group is added the Chinese herbal and crude drugs preparations 15.0g(15.0g/kg of embodiment 1), the IV group is added the Chinese herbal and crude drugs preparations 30.0g(30.0g/kg of embodiment 1), the every kilogram of feed of V group is added licorice polysaccharide 2g(2.0g/kg).5 experimental group, every group of 2 experiment pool, 1 Xiao Chi is used for experiment, and 1 Da Chi is used for the medicine pilot scale.Except medication, identical feeding.
1.2 sampling
Before each experiment, 12 of the basic identical Shelled Turtle Trionyx Sinensis of specification are got in every pond, and (the male and female ratio is: 1:1).Respectively 10,12,3, each sampling in April once.
1.3 enzyme activity detects
Coomassie brilliant blue G-250 100mg is dissolved in 50ml 95% ethanol, adds 100ml 85% H
3PO
4, distilled water diluting is to 1000ml, Filter paper filtering.Contain 0.01%(W/V in final reagent) Coomassie brilliant blue G-250,4.7%(W/V) ethanol, 8.5%(W/V) H
3PO
4
BSA cow's serum hemalbumin (work is given birth in Shanghai) is mixed with 100 μ g/ml protein solutions, production standard curve according to its purity with 0.15mol/L NaCl.
SOD and CAT vigor side are fixed, all adopt Nanjing to build up the detection kit that biotech firm provides.
2, result
2.1 the impact on superoxide dismutase (SOD)
Experimental result shows that the superoxide dismutase activity of Shelled Turtle Trionyx Sinensis liver, kidney is different, and the variation that the superoxide dismutase of Shelled Turtle Trionyx Sinensis liver, kidney is shown by the impact of Chinese herbal and crude drugs preparations, licorice polysaccharide is also inconsistent.Under the effect of Chinese herbal and crude drugs preparations and licorice polysaccharide, kidney SOD reaches maximum in experiment mid-term, descends gradually later on; Liver SOD is active significant raising just occurs to testing the later stage.To the result of experimental data statistical analysis, use the experimental group of 30.0g/kg immunopotentiator, liver SOD is active respectively organizes significant difference (F=2.4754＞F(4,116), P＜0.05 with other).The source of this species diversity is mainly the experiment later stage, the very big increase of SOD activity.Do not have significant difference between the active group of kidney SOD, but experiment SOD activity in mid-term obviously is different from other each experimental stage.The presentation of results Chinese herbal and crude drugs preparations of experiment is little to the improvement effect of Shelled Turtle Trionyx Sinensis kidney SOD activity, and the improvement of liver SOD activity is had obvious effect.
2.2 the impact on catalase (CAT)
The CAT variation tendency of kidney is more similar to SOD, and the variation of liver CAT is different from SOD fully.This may be different relevant from institutional framework, the detoxification mechanism of liver, kidney, and the effect that the activity inducement thing produces is also just different.There is not significant difference in kidney CAT activity between each experimental group, liver CAT is active significant raising just occurs to testing the later stage.Statistical analysis is shown, use the experimental group of 30.0g/kg Chinese herbal and crude drugs preparations, liver CAT is active respectively organizes significant difference (F=4.0160＞F(4,116), P＜0.05 with other).To the multiple comparison analyse of each experimental stage Shelled Turtle Trionyx Sinensis liver CAT activity, except not having significant difference between mid-term and later stage, more all deposit significant difference all the other each periods.
Result shows, Chinese herbal and crude drugs preparations centering has obvious effect to the improvement of liver SOD and CAT enzymatic activity, especially in liver organization.And strengthen with the rising of concentration.Test shows that Chinese herbal and crude drugs preparations not only can improve the activity of Shelled Turtle Trionyx Sinensis immune factor, and can promote the oxidation resistance of body.
3, concrete application implementation case
Test site is Yuhang District blue sky, Zhejiang Province Shelled Turtle Trionyx Sinensis Specialty Co-operative Organization, prepares Chinese herbal and crude drugs preparations according to the ratio of embodiment 1, adds 5 grams in one kilogram of feed.After feeding 3 months, 30 dermopathic incidences of disease of pond soft-shelled turtle of experimental group have reduced by 23.0%, and the death rate of test group is than blank group low 8.3%.Adopt embodiment 2-3 combination preparation to use, also can reach the effect of similar embodiment 1.
Claims (6)
1. Chinese herbal formula, the mass percent of described Chinese herbal and crude drugs preparations is composed as follows: fortune paulownia flower 8～12%, mother chrysanthemum 8～15%, honeysuckle 8～15%, beggar-ticks 8～15%, parasitic loranthus 8～15%, Radix Glycyrrhizae 8～15%, giant knotweed 8～15%, hawthorn 8～15%.
2. Chinese herbal formula claimed in claim 1 is used for improving the immunity of testudinate.
3. the preparation method of Chinese herbal formula claimed in claim 1, at first take respectively various raw materials according to mass percent, then raw material pulverized, and sieves, and mixing namely makes.
4. the application of Chinese herbal formula claimed in claim 1 in preparation terrapin feed addictive.
5. the application of Chinese herbal formula claimed in claim 1 in the preparation water disinfectant.
6. terrapin feed addictive, described feed addictive includes Chinese herbal formula claimed in claim 1.
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