CN102894234A - Plant immunopotentiator capable of improving immunity of Chinese soft shell turtle - Google Patents
Plant immunopotentiator capable of improving immunity of Chinese soft shell turtle Download PDFInfo
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- CN102894234A CN102894234A CN2012104241332A CN201210424133A CN102894234A CN 102894234 A CN102894234 A CN 102894234A CN 2012104241332 A CN2012104241332 A CN 2012104241332A CN 201210424133 A CN201210424133 A CN 201210424133A CN 102894234 A CN102894234 A CN 102894234A
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Abstract
The invention relates to a plant immunopotentiator capable of improving the immunity of the Chinese soft shell turtle, belonging to the technical field of feed additives. The plant immunopotentiator is characterized by consisting of the following materials by weight percentage: 15-25% of astragalus polysaccharide, 5-15% of honeysuckle, 20-30% of polygonum cuspidatum, 30-40% of glycyrrhiza polysaccharide and 5-15% of herba andrographitis. A compound preparation is formed by the polysaccharide, saponins, essential oil, organic acid and the like with the function of immune adjustment in Chinese medicinal herbs, so that the immunity of the Chinese soft shell turtle can be improved. The raw material ingredients of the plant immunopotentiator are from the natural world, so that the plant immunopotentiator is safe to people and livestock, is environment-friendly, and belongs to the green additives, therefore, the disease happening of the Chinese soft shell turtle can be reduced, and the technical support can be provided for the cultivation of the pollution-free green Chinese soft shell turtle.
Description
Technical field
The invention belongs to field of feed additive technology, be specially a kind of plant immunopotentiator that improves Shelled Turtle Trionyx Sinensis immunity.
Background technology
Shelled Turtle Trionyx Sinensis (Trionyx sinensis) is the higher special aquatic products product of a kind of economic worth, and its meat flavour is delicious, and has nourishing effects, and is well received on market.For deal with day by day serious disease, high dose ground uses all kinds of chemicalses and antibiotic in breeding process, and breeding environment and aquatic product quality have also been produced many negative effects, makes soft-shelled turtle as the vivid sustain damage of health food.Therefore, be necessary to utilize the resources of medicinal plant of China's abundant, develop a kind of Shelled Turtle Trionyx Sinensis vegetalitas immunopotentiator of green non-pollution, the harm that Shelled Turtle Trionyx Sinensis is caused to reduce disease ensures the sound development of Terrapin raising industry.
Summary of the invention
The purpose of this invention is to provide a kind of plant immunopotentiator that improves Shelled Turtle Trionyx Sinensis immunity, i.e. a kind of compound preparation that relates to several traditional Chinese medicine ingredients, said preparation has the Shelled Turtle Trionyx Sinensis of significantly improving immunity, reduces the characteristics that disease occurs.
The plant immunopotentiator of raising Shelled Turtle Trionyx Sinensis immunity of the present invention is comprised of the material of following percentage by weight: astragalus polyose 15～25%, honeysuckle 5～15%, giant knotweed 20～30%, licorice polysaccharide 30～40%, Herba Andrographitis 5～15%.
The preparation method of above-mentioned plant immunopotentiator, at first take by weighing respectively astragalus polyose 15～25%, honeysuckle 5～15%, giant knotweed 20～30%, licorice polysaccharide 30～40%, Herba Andrographitis 5～15% according to percentage by weight, sieve after grinding to form powdery, the screen cloth fineness is the 80-120 order, again powder is carried out drying and finishes preparation.
Plant immunopotentiator of the present invention is as the feed addictive of Shelled Turtle Trionyx Sinensis.
The immunopotentiator that the present invention makes, respond well, produce effects is quick, lasting medicine, low to environmental toxicity, the difficult drug resistance that produces.And preparation technology is simple, realizes easily, uses safety, convenient, easy to utilize.
The specific embodiment
The used astragalus polyose of immunopotentiator of the present invention can induce body to produce interferon, viral interference copying in body, and the immunologic function of raising body promotes hepatocuprein (SOD), the enhancing of activity of glutathione peroxidase; And can strengthen and stimulate the generation of lymphocyte and RE confluent monolayer cells, strengthen the phagocytic function of RE confluent monolayer cells and macrophage, and body fluid, mucous membrane and cellular immunity are had good promotion and regulating action.
Honeysuckle has the effects such as resisting pathogenic microbes, anti-inflammatory and analgesic, the function that strengthens the defense, antiendotoxin.
Described giant knotweed nature and flavor little hardship that is slightly cold is returned liver, courage, lung channel, and expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum effect are arranged; Licorice polysaccharide has immunological regulation, the effect such as antitumor, antiviral, and acellular toxic action.And that Herba Andrographitis has is clearing heat and detoxicating, swelling and pain relieving, antibiotic and antitumaous effect.
After being pulverized and mixed, above-mentioned five kinds of components made plant immunopotentiator of the present invention.
For a more clear understanding of the present invention and the curative effect of this immunopotentiator, the present invention is described in further detail by specific embodiment and correlation test below.
Embodiment 1
Take by weighing respectively by weight percentage astragalus polyose 15g, honeysuckle 10g, giant knotweed 20g, licorice polysaccharide 40g and Herba Andrographitis 15g, mixture is ground the rear sieve of using, wherein the fineness requirement of sieve aperture is the 80-120 order.Pulvis after filtering is carried out by drying, to pulvis weight ratio drying, reduce 10%; Thereby make required immunopotentiator.
Embodiment 2
Take by weighing respectively by weight percentage astragalus polyose 25g, honeysuckle 15g, giant knotweed 20g, licorice polysaccharide 30g and Herba Andrographitis 10g.Fineness requirement is the 80-120 order, loss on drying≤10.0%.Get required immunopotentiator.
Embodiment 3
Percentage by weight takes by weighing respectively astragalus polyose 25%, honeysuckle 18%, giant knotweed 22%, licorice polysaccharide 30%, Herba Andrographitis 5%.Fineness requirement is the 80-120 order, loss on drying≤10.0%.Get required immunopotentiator.
The using method of above-mentioned immunopotentiator and consumption are: immunopotentiator, Shelled Turtle Trionyx Sinensis powder, the suitable quantity of water of embodiment 1 or 2 preparations are placed fodder machine, add immunopotentiator 1-3 gram in the per kilogram Shelled Turtle Trionyx Sinensis powder, then preferred 2 grams that add stir into the feed of throwing something and feeding according to the fodder machine program.
The invention will be further described below in conjunction with salivary lysozyme that the plant immunopotentiator feeding experiment draws.
Embodiment 4 culture experiments:
In the raising pond in same warmhouse booth, choose at random the identical raising pond of 6 conditions as test tank.The 1# test group is 3 ponds, the feed of the plant immunopotentiator that added the embodiment of the invention 1 preparation of feeding.3 ponds of 2# test group are blank, and it is identical to throw something and feed, but do not add the feed of reinforcing agent.
Behind the predetermined 60d that throws something and feeds continuously, from each test and contrast pond, fish at random 3 Shelled Turtle Trionyx Sinensis, dry moisture, hold head with haemostatic clamp, operating scissors are cut off Cervical Vessels, and blood is stored in inherent 37 ℃ of heparin tube and placed 2 hours, treat that serum is separated out fully to be placed in 4 ℃ of refrigerators, serum keeping is used for testing index in-20 ℃:
1, serum superoxide dismutases (Superoxide Dismutase, SOD) determination of activity adopt xanthine oxidase, glutathione peroxidase GSH-PX, alkaline phosphatase (AKP) all use Nanjing build up biotechnology research kit.The AKP alkaline phosphatase records clearly light absorption value greater than 1 with pure blood, and numerical value is inadvisable, does 5 times of dilutions with physiological saline.
2, lysozyme: lysozyme standard items, micrococcus luteus powder are purchased from Nanjing and build up: the bacteria suspension that micrococcus luteus is mixed with 0.2mg/mL with the PBS of 0.1mol/L.Each sample is got 3.0 mL bacteria suspensions, gets serum 0.05mL and joins in the bacteria suspension, and mixing is measured the A0 value at once under 550nm.Then incubation 30min in 28 degree water-baths places 4 ℃ of ice bath 8min cessation reactions, measures the A value under the 550nm again.By formula U1=(A0-A)/A calculates the activity of lysozyme.
Test data is with mean value ± standard error (Mean ± SE) expression.Carry out the one-factor analysis of variance (One-way ANOVA) with SPSS11.5 software, whether have significant difference with each index mean value between check different experiments group.As there were significant differences (P＜0.05), then make the DuncanShi multiple comparison analyse.
Results and analysis:
The variation of salivary lysozyme
Add plant immunopotentiator in the feed and improve Shelled Turtle Trionyx Sinensis alkaline phosphatase enzyme activity, and there were significant differences between the control group (P＜0.05).Interpolation is organized total superoxide dismutase activity and is significantly higher than control group (P＜0.05).The activity of glutathione peroxidase does not have significant difference (P＞0.05) between each test group.The lysozyme activity of interpolation group is significantly higher than control group (P＜0.05).The result shows in the feed and to add the activity that plant immunopotentiator of the present invention can improve Shelled Turtle Trionyx Sinensis alkaline phosphatase, total superoxide dismutase, lysozyme.
Embodiment 4, actual cultivation are used
Test site is that Zhejiang Province's Yuhang District is along rising the Shelled Turtle Trionyx Sinensis Specialty Co-operative Organization, according to the plant immunopotentiator that embodiment 2 prepares, interpolation 2 grams in one kilogram of feed.After feeding 3 months, 30 pond average weights of experimental group are 400g, and the blank group is 360g9.The death rate of test group is than blank group low 5.8%.Adopt the immunopotentiator of embodiment 3 preparations also can reach beneficial effect of the present invention.
Shelled Turtle Trionyx Sinensis feed in above-described embodiment can be selected commercially available arbitrary money product.
Claims (3)
1. plant immunopotentiator that improves Shelled Turtle Trionyx Sinensis immunity, it is characterized in that described reinforcing agent is comprised of the material of following percentage by weight: astragalus polyose 15～25%, honeysuckle 5～15%, giant knotweed 20～30%, licorice polysaccharide 30～40%, Herba Andrographitis 5～15%.
2. the preparation method of plant immunopotentiator claimed in claim 1, it is characterized in that, take by weighing at first respectively astragalus polyose, honeysuckle, giant knotweed, licorice polysaccharide and Herba Andrographitis, sieve after grinding to form powdery, the screen cloth fineness is the 80-120 order, again powder is carried out drying and finishes preparation.
3. plant immunopotentiator claimed in claim 1 is as the feed addictive of Shelled Turtle Trionyx Sinensis.
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Cited By (9)
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| CN103125780A (en) * | 2013-03-25 | 2013-06-05 | 浙江省淡水水产研究所 | Compound Chinese herbal preparation for improving testudinate immunity |
| CN103478436A (en) * | 2013-10-21 | 2014-01-01 | 湖南农业大学 | Chinese medicinal herb feed additive for regulating fat metabolism of Chinese softshell turtle, promoting growth and enhancing immunity and feed containing same |
| CN103564267A (en) * | 2013-11-15 | 2014-02-12 | 桂平市生产力促进中心 | Forage having effects of preventing and treating parotitis of palea steindachneri |
| CN104206696A (en) * | 2014-09-26 | 2014-12-17 | 张可池 | Ground beetle feed |
| CN104306697A (en) * | 2014-09-30 | 2015-01-28 | 湖北宏旺生态农业科技有限公司 | Growth-promoting granule traditional Chinese medicine composition for cultivating and breeding Chinese softshell turtle fine breed |
| CN104381616A (en) * | 2014-12-14 | 2015-03-04 | 河南联合英伟饲料有限公司 | Medicinal core type feed particles capable of improving immunity and preparation method for medicinal core type feed particles |
| CN104799007A (en) * | 2015-01-15 | 2015-07-29 | 南宁市春华农业科技有限公司 | Chinese herbal medicine additive for enhancing disease resistance of Truogx sinensis and preparation method thereof |
| CN105360597A (en) * | 2015-06-18 | 2016-03-02 | 合肥市畜牧水产技术推广中心 | Functional medicinal bait for improving quality of soft-shelled turtles and preparation method and feeding method thereof |
| CN112913975A (en) * | 2021-03-02 | 2021-06-08 | 浙江万里学院 | Feed additive for improving immunity of Chinese softshell turtles |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125780A (en) * | 2013-03-25 | 2013-06-05 | 浙江省淡水水产研究所 | Compound Chinese herbal preparation for improving testudinate immunity |
| CN103125780B (en) * | 2013-03-25 | 2014-05-07 | 浙江省淡水水产研究所 | Compound Chinese herbal preparation for improving testudinate immunity |
| CN103478436A (en) * | 2013-10-21 | 2014-01-01 | 湖南农业大学 | Chinese medicinal herb feed additive for regulating fat metabolism of Chinese softshell turtle, promoting growth and enhancing immunity and feed containing same |
| CN103478436B (en) * | 2013-10-21 | 2014-08-13 | 湖南农业大学 | Chinese medicinal herb feed additive for regulating fat metabolism of Chinese softshell turtle, promoting growth and enhancing immunity and feed containing same |
| CN103564267A (en) * | 2013-11-15 | 2014-02-12 | 桂平市生产力促进中心 | Forage having effects of preventing and treating parotitis of palea steindachneri |
| CN104206696A (en) * | 2014-09-26 | 2014-12-17 | 张可池 | Ground beetle feed |
| CN104306697A (en) * | 2014-09-30 | 2015-01-28 | 湖北宏旺生态农业科技有限公司 | Growth-promoting granule traditional Chinese medicine composition for cultivating and breeding Chinese softshell turtle fine breed |
| CN104306697B (en) * | 2014-09-30 | 2018-12-28 | 湖北宏旺生态农业科技有限公司 | One kind dissipating Chinese medicine composition with growth promoting for improved variety of China turtle cultivation, breeding |
| CN104381616A (en) * | 2014-12-14 | 2015-03-04 | 河南联合英伟饲料有限公司 | Medicinal core type feed particles capable of improving immunity and preparation method for medicinal core type feed particles |
| CN104799007A (en) * | 2015-01-15 | 2015-07-29 | 南宁市春华农业科技有限公司 | Chinese herbal medicine additive for enhancing disease resistance of Truogx sinensis and preparation method thereof |
| CN105360597A (en) * | 2015-06-18 | 2016-03-02 | 合肥市畜牧水产技术推广中心 | Functional medicinal bait for improving quality of soft-shelled turtles and preparation method and feeding method thereof |
| CN105360597B (en) * | 2015-06-18 | 2018-12-07 | 合肥市畜牧水产技术推广中心 | A kind of functional pharmaceutical chemistry and preparation method thereof and feeding method promoting soft-shelled turtle quality |
| CN112913975A (en) * | 2021-03-02 | 2021-06-08 | 浙江万里学院 | Feed additive for improving immunity of Chinese softshell turtles |
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Application publication date: 20130130 |