CN105053737A - High-efficiency pig feed additive - Google Patents
High-efficiency pig feed additive Download PDFInfo
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Abstract
The invention provides an environment-friendly pig feed additive product. The environment-friendly pig feed additive product comprises the following components in parts by weight: 8-15 parts of compound polysaccharide extract, 8-20 parts of an oceanic red yeast fermentative substance, 5-10 parts of epimedium extract, 3-8 parts of liquorice, 3-8 parts of hawthorn fruit, 1-5 parts of honeysuckle and 1-5 parts of dandelion, wherein the compound polysaccharide extract is obtained from astragalus, wolfberry and alfalfa, and the preservation number of oceanic red yeast is CCTCC NO:M2014592. The green pig feed additive product has the advantages that the oceanic red yeast can produce carotenoid with high yield and also can enrich copper, yield of carotenoid and copper content of the yeast are effectively improved by adopting a static cultivation method, the oceanic red yeast is applied to feed as a feed additive, can provide carotenoid, B-vitamins and copper ions, replaces inorganic copper ions used in the conventional feed due to high-efficiency copper enriching capability, is good in stability and can replace antibiotic to be used in feed through compatibility of Chinese herbal medicines, safety of a pork product is improved, organism immunity of a pig can be enhanced, intestinal health is regulated, metabolism function and fertility of the pig are improved, and feeding benefit is improved.
Description
Technical field
The invention belongs to feed additive field, particularly relate to a kind of pig efficient green feedstuff additive product that can improve efficiency of feed utilization and animal body immunity and reproductive capacity and preparation method thereof.
Background technology
Current aquaculture is common, and to there is efficiency of feed utilization not high, and not exclusively, keeping is not good at causing feed moisture absorption to be gone mouldy or the problem such as infested, and the existence of these problems not only considerably increases feeding cost, and result also in the wasting of resources in nutritional labeling digestion.
Solve the problem, control aquaculture cost, reduce the large bottleneck that cultivation risk is aquaculture fast development.Use chemical synthesis additive can cause animal durability, the problem such as residual, harm humans is healthy, also has a large amount of excretas simultaneously, as the pernicious gas such as ammonia, hydrogen sulfide, and contaminated environment.Chinese herbal feed additive, as the focus of emerging research, has the advantages such as natural, nutrition, side effect be little.Research shows, many Chinese herbal medicines inherently have good In Vitro Bacteriostasis ability, Chinese herbal feed antibiotic health care agent noresidue, to have no drug resistance, its mechanism of action is not only the inhibitory or killing effect to pathogenic microorganism, the more important thing is the adjustment to body disease-resistant repair ability, namely improve the anti-stress ability of body, improve body's immunity and Defense response function, thus reach the object improving meat products quality and improve animal feed utilization ratio.
Chinese herbal medicine is some plants with given efficacy, these plants are each powerful, the effectiveness of such as Activities of Some Plants is as follows: (1) barrenwort: be that China is widely used, with a long history, a kind of autonomic drug with very great development potentiality, be recorded in Shennong's Herbal the earliest.Containing bioactive ingredients such as flavonoids, polysaccharide, alkaloids in barrenwort.Motherland's medical science is thought, effect that barrenwort has " beneficial vital essence, hard muscles and bones mend waist knee, cardiac stimulant power ".Modern medicine study also confirms, barrenwort has adjustment patrogenesis, stops the physiological functions such as osteoporosis, immunity moderation, anti-oxidant, anti-ageing, antitumor, anti-hepatotoxin, vasodilator.Except its conventional use tonifying kidney and strengthening yang wines used as antirheumatic, clinically, barrenwort is also usually used in the diseases such as treatment osteoporosis, climacteric metancholia, lump in breast, hypertension, coronary heart disease.Take barrenwort as the Chinese herbal feed additive of primary raw material preparation, have growth promoting effects, put on weight, voluminous egg, meat, control phlegm is sick, feature reliable for effect.Its range of application mainly contains: improve efficiency of feed utilization, growth promoting effects, improves daily gain and egg production; Prevention animal dysentery, the disease such as to have loose bowels, have blood in stool.On veterinary clinic, barrenwort is mainly used in preventing and treating livestock and poultry intestinal canal diseases, treatment wind-cold-dampness arthralgia, treatment chronic cough, treatment impotence, involuntary emission etc.In addition, barrenwort, in animal breeds, also plays an important role, and can promote the secretion of endometrial thickness and ovarian hormone.(2) astragalus polyose (AstragalusPolysacharin, APS) astragalus polyose is the main component that the Radix Astragali plays a role.The Radix Astragali has effect that invigorating qi for consolidating superficies, arrest sweating are admittedly de-, hold in the palm sore myogenic, inducing diuresis for removing edema.Be used for the treatment of the deficiency of vital energy weak, the sinking of qi of middle-jiao, rush down prolapse of the anus for a long time, uterine bleeding of having blood in stool, exterior deficiency spontaneous perspiration, ulcer difficulty is burst, and burst for a long time and do not hold back, the deficiency of blood is sallow, and interior heat is quenched one's thirst, chronic nephritis, albuminuria, diabetes etc.Astragalus polyose is made up of hexuronic acid, glucose, fructose, rhamnose, arabinose, galacturonic acid and glucuronic acid etc., can be used as immunopotentiating agent or conditioning agent, there is antiviral, antitumor, anti-ageing, radioresistance, resisting stress, the effect such as anti-oxidant simultaneously.(3) LBP-X (Lyciumbarbarumpolysaccharides, LBP) be a kind of water-soluble polysaccharide, clearly this polysaccharide system proteoglycan, molecular weight is 22 ~ 25kD, is made up of arabinose, glucose, vinegar polysaccharide, galactolipin, mannose, wood sugar, rhamnose 6 kinds of monosaccharide components.LBP obviously can increase mouse peripheral T lymphocyte percentage, and its effect is strong, and consumption is little, and have selectively acting to the reaction of T lymphocyte immunity, it is a kind of T lymphocyte immunity adjuvant.LBP also has facilitation to humoral immunity.LBP has restitution to the suppressed of the immunologic function of irradiated mice, and the mouse immune gone back caused by reversible hydrocortisone is low, makes thymic factor D injection and antibody tormation increase (Li Xuejia, 1994).Wang Yongji (2004) research shows, adds the antalzyme activity that 45mg/kg LBP-X significantly can strengthen crucian, improve its non-specific immunity in feed.(4) Alfalfa Polysaccharide (alfalfapolysaccharides, APS) be the active polysaccharides from plants extracted in leguminous forage alfalfa, stable in properties, containing glucose, mannose, rhamnose isoreactivity composition, be converted by the growth and lymphocytic propagation promoting fowl poultry immune organ and improve the immunologic function of livestock and poultry, play antioxidation by scavenging free radicals.Alfalfa Polysaccharide also has the various biological functions such as antitumor, anti-infective, reducing blood lipid, hypoglycemic, antibacterial, anti-inflammatory in addition.(5) Radix Glycyrrhizae: there is in Radix Glycyrrhizae bioactive active ingredient and mainly comprise triterpene saponin, flavonoids, polysaccharide, Coumarins, alkaloids and amino acid etc.Radix Glycyrrhizae has clearing heat and detoxicating, logical muscle arteries and veins, sharp vim and vigour, quench the thirst eliminate the phlegm, tonifying spleen and the effect such as stomach, coordinating the drug actions of a prescription.Radix Glycyrrhizae is applied in feed can improve growth of animal performance, enhanced machine ability of immigrants, improve intestinal health, reduce stress reaction etc.(6) Dandelion antipyretic and antidotal type famous medicine, for preventing and treating the key medicine of sore Huang, acute mastitis, red eye, swell pain.Modern medicine study is thought, it not only containing multiple nutrients material, can be used as the use of people's health-care vegetable, and be sterilizing and anti-virus, anti-inflammatory staple.As animal feed additive, having growth promotion, provide animal product quality, the effects such as disease preventing and treating, is that medicine raises dual-purpose, nuisanceless green feed addictive.
Carotenoid is a class in Polyenes that is yellow, orange or red, and it is the most ubiquity of occurring in nature is also the most stable natural colouring matter, is extensively present in many animals and plants.Carotenoid as a kind of excellent food additives and the nutritional supplement improving human nutrition, its fine quality and effect, for a long time just generally acknowledge by countries in the world, be described as most promising antioxidant.At present, carotenoid is widely used in the industries such as food, medicine, cosmetics and feed addictive, the application prospect good due to it and the function of brilliance, for a long time extremely people's favor.
Extract carotenoid complex process from natural food, cost is high and wastes abundance of food, therefore become study hotspot by Production by Microorganism Fermentation carotenoid, be particularly widely studied as bacterial classification with not toxigenic rhodotorula.Rhodotorula is the one in saccharomycete, belongs to stealthy ball Saccharomycetaceae, is unicellular fungi.Rhodotorula body is oval or ellipse, and mode of reproduction is polygon gemmation, can produce significantly red or xanthein.Rhodotorula can produce the multiple pigments such as beta carotene, torulin and torularhodin.
Copper is the required trace element of animal, all plays a very important role for the health of animal and growth.At present, existing many tests confirm, the copper adding (250mg/kg) in Weaned Growing Pig Diets using copper sulphate as copper source can improve the same feed intake of piglet, daily gain and meat feed ratio.But the copper sulphate adding this inorganic states in feed also exists a lot of problem, as low in pig absorptivity, with other component compatibility in diet is poor, excretion rate is high, contaminated environment etc.But saccharomycete has huge legendary turtle and closes the ability of copper particle, and it is except the copper containing high-load and high-absorbility, also have good stability, and diet in good, the advantage such as absorptivity is high, excretion rate is low, environmental pollution is little of other component compatibility.
It is significant to feed additive industry development that effective exploitation utilizes natural Chinese medicinal herb material or fungi to obtain green feed additive product.
Summary of the invention
The object of the invention is to provide a kind of intestinal health improving pig, improves the immunity of organisms of pig and fertility, improves the green feed additive product of efficiency of feed utilization, its composed as follows according to parts by weight:
Complex polysaccharide extract 8-15, ocean rhodotorula fermentation culture medium 8-20, Shorthorned Epimedium P.E 5-10, Radix Glycyrrhizae 3-8, hawthorn 3-8, honeysuckle 1-5, dandelion 1-5; Described complex polysaccharide extract is from the Radix Astragali, matrimony vine and clover; Described ocean rhodotorula deposit number is CCTCCNO:M2014592.
Preferred parts by weight consist of further:
Complex polysaccharide extract 10-12, ocean rhodotorula fermentation culture medium 10-15, Shorthorned Epimedium P.E 6-8, Radix Glycyrrhizae 4-6, hawthorn 4-6, honeysuckle 2-3, dandelion 2-3.
The preparation method of described complex polysaccharide extract is as follows:
The raw material Radix Astragali, alfalfa and matrimony vine drying and crushing are crossed 60 mesh sieves, add crushed material gross weight 5-8 times of boiling water, lixiviate 2-3 each 20-40min, filters and is mixed by leaching liquor, naturally cool to room temperature, absolute ethyl alcohol is added in leaching liquor, concentration of alcohol in mixed liquor is made to reach 70-85%, 10 DEG C of airtight refrigeration precipitation 6-8 hour, sediment vacuum drying, pulverize 60 mesh sieves, obtain complex polysaccharide extract.
The raw material Radix Astragali, matrimony vine and clover can mix by arbitrary proportion, and preferred mass ratio is 1-15:1-15:1-5, and preferred mass ratio is 1-12:1-6:1-3.
The preparation method of described Shorthorned Epimedium P.E is as follows:
After barrenwort pulverized 40-50 mesh sieve, add 50% ethanolic solution of barrenwort 8-10 times of weight, extract 2 times with 40-50kHz ultrasonic assistant under room temperature condition, each 30min, filter to obtain ethanol extract, add 5-8 times of weight hot water in residue after extraction, 80 DEG C decoct 1h, and after filtering, solution mixes with ethanol extract, straight fire heating concentrated extracting solution, conventional method dry, pulverize 60 mesh sieves, obtained complex polysaccharide extract.
Described ocean rhodotorula fermentation culture medium is prepared by the following method:
Preferred fermentation medium consists of glucose 1%, enzymolysis corn flour 1-3%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 150-200mg/L, sodium chloride 20%, and all the other are water; Initial pH is 8, inoculum concentration 3-8%, and shaking speed is 50-80r/min, 24-28 DEG C and cultivates and to adjust temperature after 10-15 hour and be 16-20 DEG C and carry out low temperature static gas wave refrigerator, and stop stirring, pH is adjusted to 3-5, keeps 2-4 hour; Stairstepping is warmed up to 26-30 DEG C afterwards, and pH is adjusted to 4.6-5.5, and add peptone according to the addition of initial medium 2.5%, the amount according to 2% adds dusty yeast, and shaking speed is 50-100r/min, continues fermentation 10-15h; Namely zymotic fluid drying and crushing obtains marine yeast fermentation culture medium after crossing 50-60 mesh sieve.
Preferably, in culture medium, the methionine chelate copper of initial medium weight 3-5wt% is added in the described low temperature static gas wave refrigerator stage.
The preparation method of described enzymolysis corn flour is: mixed with water by corn flour, wet pulverizing prepares trickle corn flour feed liquid, add AMS and calcium chloride, regulate pH5.5-6.0,72 DEG C of insulation 15min, then be heated to 90 DEG C of enzymolysis 20-30min, then 55-60 DEG C is cooled to, regulate pH4.5-5.0, add acid protease hydrolysis 30-40min, after enzymolysis liquid centrifuge washing, namely obtain concentrated enzymolysis corn flour.
The preparation method of feed addictive of the present invention is as follows:
By hawthorn, Radix Glycyrrhizae, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, then to mix in proportion with ocean rhodotorula fermentation culture medium, complex polysaccharide extract and the Shorthorned Epimedium P.E obtained by said method.
A strain provided by the invention is used for the ocean rhodotorula WYN1 of feed addictive, belong to Rhodotorula (Rhodotorula), described bacterial strain on November 25th, 2014 be preserved in China typical culture collection center (address: China. Wuhan. Wuhan University, postcode 430072), deposit number is: CCTCCNO:M2014592, Classification And Nomenclature: ocean rhodotorula WYN1 (Rhodotorulasp.WYN1).
Feedstuff additive product of the present invention all can add use in general livestock and poultry and aquatic feeds, and especially in pig feed, result of use is best, and addition is advisable with feed relative 1-5 ‰.
Beneficial effect:
The present invention utilize be separated from marine environment the strain that obtains can High Yield of Carotenoid again can the ocean rhodotorula WYN1 of enriching Cu, effectively raise carotenoid output and copper content in yeast by static gas wave refrigerator method.Adopt the inventive method fermented and cultured ocean rhodotorula WYN1, in its biomass, carotenoid output and born of the same parents, copper content reaches more than 27g/L, the dry bacterium of 35-40mg/L and 7240-8000 μ g/g respectively.In research and development, inventor surprisingly finds that low temperature static gas wave refrigerator effectively can improve carrot and copper content, is important achievement of the present invention.
Ocean rhodotorula WYN1 of the present invention is applied in feed as feed addictive, carotenoid, B family vitamin and copper ion can be provided, its efficient rich copper ability instead of the Inorganic Copper ion used in conventional feed, good stability well, is easy to the permanently effective storage of feed and transport, good with other component compatibility in diet, absorptivity is high, and excretion rate is low, and environmental pollution is little, improve animal skin quality and hair color, the nutritive value of fortified feed.
The present invention is simply thorough to the extracting method of barrenwort and complex polysaccharide, greatly saves ethanol consumption and extraction time, and ensure that icariin and flavones in extract, or the active constituent content such as astragalus polyose, Alfalfa Polysaccharide, LBP-X.
The present invention is composite by science, the ocean rhodotorula bacterium and multiple medicinal herb components with rich copper ability and High Yield of Carotenoid are achieved organic assembling, effectively can reduce the use amount of antibiotics in letting animals feed, improve the security of pork product, improve food utilization efficiency, strengthen the immunity of organisms of pig, regulating intestinal canal is healthy, improve metabolism and the fertility of pig, improve raise benefit.
Detailed description of the invention
The inventive method is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Embodiment 1: the acquisition of ocean rhodotorula bacterium WYN1 and the domestication of resistance to copper ability
(1) by gathering the fresh biological specimen such as extra large shrimp, starfish, marine alga from Huanghai Sea seashore, be placed in aseptic YEPD culture medium, picking redness or pink circular colonies, be further purified cultivation.According to the accumulation of each bacterial classification carotenoid, the bacterial classification that screening carotenoid output is high.The ocean rhodotorula WYN1 that wherein carotenoid output is higher, cell is oval, does not have pseudohypha; The YEPD culture medium of seawater configuration forms red colonies, smooth surface, neat in edge; Product spore culture medium does not produce ascospore, and polygon budding, without ballistopore; Azymic sugar, does not form kind of starch compound, does not assimilate inositol.According to These characteristics, tentatively determine to belong to Rhodotorula (Rhodotorula).Inclined-plane is stored in refrigerator.
(2) fermented and cultured of ocean rhodotorula WYN1
Activate on strain transfer to slant medium, after 28 DEG C of cultivation 24h, be inoculated in liquid seed culture medium, 28 DEG C of shaken cultivation 36h obtain seed liquor, be equipped with in the 250mL triangular flask of 60mL fermentation medium by 10% inoculum concentration access, 28 DEG C, shaken cultivation 48h (shaking speed is 180r/min).Fermentation medium consists of glucose 2%, peptone 1%, yeast extract 1%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, salinity 20.48h cultivated by 28 DEG C of shaking tables, and rotating speed is 180r/min.
(3) resistance to copper ability domestication
Ocean rhodotorula bacterium WYN1 being inoculated in content of copper ion is in 50mg/L (copper sulphate configuration) culture medium, is placed in 28 DEG C, and on 180r/min constant-temperature table, 24h is cultivated in concussion.Then from this culture, get 3ml fermentate is inoculated in the malt extract medium of not cupric, cultivates 24h.Repeatedly cultivate several generations, yeast is grown in the culture medium of cupric stable, and when quantity no longer increases, more progressively improve the copper concentration of culture medium.So saccharomycete repeatedly just can be made can to adapt to the environment that content of copper ion is 300mg/l, obtain the characteristic of resistance to copper.The present invention, after ocean rhodotorula being carried out to the domestication of resistance to copper ability, can improve cellular biomass, the output of carotenoid and the rich copper ability of yeast effectively.
(4) mensuration of cellular biomass
By centrifugal for zymotic fluid 3000r/min 10min, abandon supernatant, thalline is again centrifugal after aseptic washing 2 ~ 3 times, and gained thalline is placed in 60 DEG C of baking ovens and is dried to constant weight, correct amount.
(5) extraction of carotenoid:
1g dry mycelium is put into 100mL triangular flask, and add the hydrochloric acid soaking at room temperature 1h of 5mL3mol/L, boiling water bath 4min, cools rapidly, and 3000r/min is centrifugal, and 15min is precipitated as cell residue, is settled to 20mL extraction, obtains carotenoid leaching liquor with acetone.
(6) mensuration of carotenoid
After suitably being diluted by extract, under 475nm condition, measure absorbance value with 722 type spectrophotometers.Be calculated as follows carotenoid content:
In formula: A λ
maxfor the absorbance at 475nm wavelength place; D is extension rate when measuring sample; V is acetone consumption (mL); 0.16 is the molar extinction coefficient of carotenoid; W is rhodotorula dry cell weight (g).
(7) yeast copper content measures
Use aas determination.
(8) culture presevation
The above-mentioned rich copper ocean rhodotorula WYN1 through domestication is preserved in China typical culture collection center, and deposit number is: CCTCCNO:M2014592.
Embodiment 2:(contrast test) Fermentation Conditions of ocean rhodotorula WYN1 High Yield of Carotenoid
Fermentation medium forms: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg/L (copper sulphate configuration), sodium chloride 20%, and all the other are water; Initial pH is 8, inoculum concentration 10%, and shaking speed is 180r/min, and cultivate after 24 hours for 28 DEG C, temperature is adjusted to 30 DEG C, and pH is adjusted to 5, adds a nitrogenous source, namely adds peptone according to the addition of 2.5% again, continues fermentation 24h.Adopt said method fermented and cultured ocean rhodotorula WYN1, in its biomass, carotenoid output and born of the same parents, copper content is respectively 27.52g/L, 25.12mg/L and the dry bacterium of 6240 μ g/g.
Embodiment 3: feed addictive and preparation thereof
The parts by weight of feed addictive are composed as follows:
Complex polysaccharide extract 10, ocean rhodotorula fermentation culture medium 15, Shorthorned Epimedium P.E 6, Radix Glycyrrhizae 5, hawthorn 5, honeysuckle 2, dandelion 3; Described ocean rhodotorula deposit number is CCTCCNO:M2014592.
The preparation method of described complex polysaccharide extract is as follows:
The raw material Radix Astragali, matrimony vine, clover are crossed 60 mesh sieves with mass ratio 4:2:1 drying and crushing, add crushed material gross weight 8 times of boiling water, lixiviate 2 each 30min, filter and are mixed by leaching liquor, naturally cool to room temperature, absolute ethyl alcohol is added in leaching liquor, concentration of alcohol in mixed liquor is made to reach 70%, 10 DEG C of airtight refrigeration precipitations 8 hours, sediment vacuum drying, pulverize 60 mesh sieves, obtain complex polysaccharide extract.In extract, polysaccharide total amount reaches more than 90%.
The preparation method of described Shorthorned Epimedium P.E is as follows:
After barrenwort pulverized 40-50 mesh sieve, add 50% ethanolic solution of barrenwort 8 times of weight, extract 2 times with 50kHz ultrasonic assistant under room temperature condition, each 30min, filter to obtain ethanol extract, add 8 times of weight hot water in residue after extraction, 80 DEG C decoct 1h, and after filtering, solution mixes with ethanol extract, straight fire heating concentrated extracting solution, spraying dry, pulverized 60 mesh sieves, obtained complex polysaccharide extract.
The preparation method of described ocean rhodotorula fermentation culture medium is as follows:
Fermentation medium consists of glucose 1%, enzymolysis corn flour 2%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 180mg/L (copper sulphate configures, and calculates with copper ion), sodium chloride 20%, and all the other are water; Initial pH is 8, inoculum concentration 5%, and shaking speed is 60r/min, and cultivate for 26 DEG C and to adjust temperature after 12 hours and be 18 DEG C and carry out low temperature static gas wave refrigerator, stop stirring, pH is adjusted to 4, keeps 3 hours; A 2-4 per hour DEG C stairstepping is warmed up to 28 DEG C afterwards, and pH is adjusted to 5.3, and the addition according to 2.5% adds peptone, and the amount according to 2% adds dusty yeast; Shaking speed is 80r/min, continues fermentation 12h.Namely zymotic fluid drying and crushing obtains marine yeast fermentation culture medium after crossing 50-60 mesh sieve.
Preferably, in culture medium, sterilizing methionine chela and the copper of culture medium weight 4wt% is added in the low temperature static gas wave refrigerator stage.
The preparation method of described enzymolysis corn flour is: mixed with water by corn flour, wet pulverizing prepares trickle corn flour feed liquid, add AMS and calcium chloride, regulate pH5.5-6.0,72 DEG C of insulation 15min, then be heated to 90 DEG C of enzymolysis 20min, then 55 DEG C are cooled to, regulate pH4.5-5.0, add acid protease hydrolysis 40min, after enzymolysis liquid centrifuge washing, namely obtain concentrated enzymolysis corn flour.
Adopt said method fermented and cultured ocean rhodotorula WYN1, in its biomass, carotenoid output and born of the same parents, copper content is respectively more than 28g/L, 38mg/L and the dry bacterium of 7650 μ g/g.
The preparation method of feed addictive is as follows:
By Radix Glycyrrhizae, hawthorn, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, then to mix in described ratio with ocean rhodotorula fermentation culture medium, complex polysaccharide extract and the Shorthorned Epimedium P.E obtained by said method
Embodiment 4: feed addictive and preparation thereof
The parts by weight of feed addictive are composed as follows:
Complex polysaccharide extract 12, ocean rhodotorula fermentation culture medium 20, Shorthorned Epimedium P.E 10, Radix Glycyrrhizae 8, hawthorn 8, honeysuckle 5, dandelion 8; Described ocean rhodotorula deposit number is CCTCCNO:M2014592.
The preparation method of described complex polysaccharide extract is as follows:
The raw material Radix Astragali, matrimony vine, clover are crossed 60 mesh sieves with mass ratio 5:5:1 drying and crushing, add crushed material gross weight 6 times of boiling water, lixiviate 3 each 30min, filter and are mixed by leaching liquor, naturally cool to room temperature, absolute ethyl alcohol is added in leaching liquor, concentration of alcohol in mixed liquor is made to reach 85%, 10 DEG C of airtight refrigeration precipitations 6 hours, sediment vacuum drying, pulverize 60 mesh sieves, obtain complex polysaccharide extract.
The preparation method of described ocean rhodotorula fermentation culture medium is as follows:
Fermentation medium consists of glucose 1%, enzymolysis corn flour 1%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 200mg/L, sodium chloride 20%, and all the other are water; Initial pH is 8, inoculum concentration 8%, and shaking speed is 80r/min, and cultivate for 24 DEG C and to adjust temperature after 10 hours and be 16 DEG C and carry out low temperature static gas wave refrigerator, stop stirring, pH is adjusted to 5, keeps 2 hours; Stairstepping is warmed up to 26 DEG C afterwards, and pH is adjusted to 5.5, and the addition according to 2.5% adds peptone, and the amount according to 2% adds dusty yeast; Shaking speed is 50r/min, continues fermentation 15h.Namely zymotic fluid drying and crushing obtains marine yeast fermentation culture medium after crossing 50-60 mesh sieve.
Preferably, in culture medium, methionine chela and the copper of culture medium weight 3wt% is added in the low temperature static gas wave refrigerator stage.
The preparation method of described enzymolysis corn flour is: mixed with water by corn flour, wet pulverizing prepares trickle corn flour feed liquid, add AMS and calcium chloride, regulate pH5.5-6.0,72 DEG C of insulation 15min, then be heated to 90 DEG C of enzymolysis 30min, then 60 DEG C are cooled to, regulate pH4.5-5.0, add acid protease hydrolysis 30min, after enzymolysis liquid centrifuge washing, namely obtain concentrated enzymolysis corn flour.
Adopt said method fermented and cultured ocean rhodotorula WYN1, in its biomass, carotenoid output and born of the same parents, copper content is respectively more than 27g/L, 35mg/L and the dry bacterium of 7350 μ g/g.
The preparation method of described Shorthorned Epimedium P.E is as follows:
After barrenwort pulverized 40-50 mesh sieve, add 50% ethanolic solution of barrenwort 10 times of weight, extract 2 times with 40kHz ultrasonic assistant under room temperature condition, each 30min, filter to obtain ethanol extract, add 5 times of weight hot water in residue after extraction, 80 DEG C decoct 1h, and after filtering, solution mixes with ethanol extract, straight fire heating concentrated extracting solution, conventional method dry, pulverize 60 mesh sieves, obtained complex polysaccharide extract.
The preparation method of feed addictive is as follows:
By Radix Glycyrrhizae, hawthorn, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, then to mix in described ratio with ocean rhodotorula fermentation culture medium, complex polysaccharide extract and the Shorthorned Epimedium P.E obtained by said method
Embodiment 5: feed addictive and preparation thereof
The parts by weight of feed addictive are composed as follows:
Complex polysaccharide extract 15, ocean rhodotorula fermentation culture medium 8, Shorthorned Epimedium P.E 5, Radix Glycyrrhizae 3, hawthorn 6, honeysuckle 3, dandelion 1; Described ocean rhodotorula deposit number is CCTCCNO:M2014592.
The preparation method of described complex polysaccharide extract is as follows:
The raw material Radix Astragali, matrimony vine, clover are crossed 60 mesh sieves with mass ratio 8:12:5 drying and crushing, add crushed material gross weight 7 times of boiling water, lixiviate 2 each 40min, filter and are mixed by leaching liquor, naturally cool to room temperature, absolute ethyl alcohol is added in leaching liquor, concentration of alcohol in mixed liquor is made to reach 80%, 10 DEG C of airtight refrigeration precipitations 7 hours, sediment vacuum drying, pulverize 60 mesh sieves, obtain complex polysaccharide extract.
Described ocean rhodotorula fermentation culture medium is prepared by the following method:
Fermentation medium consists of glucose 1%, enzymolysis corn flour 3%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 150mg/L (copper sulphate configures, and calculates with copper ion), sodium chloride 20%, and all the other are water; Initial pH is 8, inoculum concentration 3%, and shaking speed is 50r/min, and cultivate for 28 DEG C and to adjust temperature after 15 hours and be 20 DEG C and carry out low temperature static gas wave refrigerator, stop stirring, pH is adjusted to 3, keeps 4 hours; Stairstepping is warmed up to 30 DEG C afterwards, and pH is adjusted to 4.8, and add peptone according to the addition of initial medium 2.5%, the amount according to 2% adds dusty yeast, and shaking speed is 100r/min, continues fermentation 10h; Namely zymotic fluid drying and crushing obtains marine yeast fermentation culture medium after crossing 50-60 mesh sieve.
Preferably, in culture medium, the methionine chelate copper of initial medium weight 5wt% is added in the described low temperature static gas wave refrigerator stage.
The preparation method of described enzymolysis corn flour is with embodiment 4.
The preparation method of described Shorthorned Epimedium P.E is as follows:
After barrenwort pulverized 40-50 mesh sieve, add 50% ethanolic solution of barrenwort 9 times of weight, extract 2 times with 40kHz ultrasonic assistant under room temperature condition, each 30min, filter to obtain ethanol extract, add 6 times of weight hot water in residue after extraction, 80 DEG C decoct 1h, and after filtering, solution mixes with ethanol extract, straight fire heating is concentrated into lucky evaporate to dryness, naturally dry, pulverized 60 mesh sieves, obtain complex polysaccharide extract.
The preparation method of feed addictive is as follows:
By Radix Glycyrrhizae, hawthorn, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, then to mix in described ratio with ocean rhodotorula fermentation culture medium, complex polysaccharide extract and the Shorthorned Epimedium P.E obtained by said method
Embodiment 6: feed addictive and preparation thereof
The parts by weight of feed addictive are composed as follows:
Complex polysaccharide extract 8, ocean rhodotorula fermentation culture medium 10, Shorthorned Epimedium P.E 8, Radix Glycyrrhizae 6, hawthorn 3, honeysuckle 1, dandelion 5; Described ocean rhodotorula deposit number is CCTCCNO:M2014592.
The preparation method of feed addictive is as follows:
By Radix Glycyrrhizae, hawthorn, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, the preparation method of ocean rhodotorula fermentation culture medium, complex polysaccharide extract and Shorthorned Epimedium P.E is basic identical with embodiment 3, and each component mixes in proportion.
Embodiment 7: product fungistatic effect is tested
Feedstuff additive product of the present invention have good suppression Escherichia coli, staphylococcus aureus, etc. the characteristic of pathogenic bacteria.Adopt the fungistatic effect of cylinder plate method to feedstuff additive product to be studied, test adopts 10 times of water extracts of feed addictive to study.Experimental result is in table 1, and result shows that product of the present invention has the superperformance suppressing pathogenic bacteria growth.
The fungistatic effect of table 1 feedstuff additive product
| Pathogenic bacteria | Staphylococcus aureus | Escherichia coli |
| Antibacterial circle diameter (mm) | 30 | 36 |
The stable performance of product of the present invention, use safety, with other feed addictive all without incompatibility.The addition of product of the present invention in feed is 1-5 ‰, and it completely can substitute antibiotics medicine, and noresidue, free from environmental pollution.After feeding, significantly can strengthen immunity and the premunition of animal body, growth promoting effects, improve daily gain and feed conversion rate, and have resisting stress, antioxidant effect; There is stronger prevention effect to intestinal bacteriosis simultaneously.According to test, the aquatic products of feeding feed addictive of the present invention, quality improves, and meet the greenization production requirement of animal food, Social benefit and economic benefit is very remarkable.
Embodiment 8: product result of use
Select Ningxia feed corporation,Ltd subordinate pig farm, select the healthy sow of age in days, body weight close (deviation≤0.1kg), piglet and each 60 of boar, all random equivalent is divided into 2 groups (test group and control groups), test group adds the obtained feedstuff additive product of the embodiment of the present invention 1, and control group does not add product of the present invention; The addition of product of the present invention is the 1-5 ‰ of feed consumption, feeds continuously 60 days.
Compared with control group, use invention feed addictive can obtain following effect:
(1) number born of sow on average increases by 1.4;
(2) boar sperm number on average increases by 38%, improves conception rate;
(3), before and after childbirth, prevention of sow constipation number of times reduces more than 36%;
(4) weanling pig weight average increases 8.6kg/ head, improves 21.1% than control group (average 7.1kg/ head);
(5) grice diarrhoea rate on average reduces by 70%, and the death rate reduces by 90%;
(6) antibiotic usage amount decreases more than 98% the piglet phase;
(7) significantly feedstuff-meat ratio average more than 24% is reduced.
In addition, carry out sensory evaluation scores to the colour of skin, the hair condition testing piglet, " 0 point " represents that the colour of skin is pale, staple length, " 1 point " represents that the colour of skin is more ruddy, hair is longer, " 2 points " represent that the colour of skin is very ruddy, hair is short, and be before feeding experiment starts " 0 point ", off-test appraisal result is as follows:
| Scoring | Control group | Experimental group | Deviation |
| The colour of skin | 0.85 | 2.0 | 135％ |
| Hair condition | 1.08 | 2.0 | 85.2％ |
Result of the test shows, product of the present invention has clear improvement to the hair color outward appearance in piglet growth process, and the colour of skin is ruddy, hair is short and smooth glossy, and this is relevant with the nutritional characteristic that the compatibility of product of the present invention rationally especially adds ocean rhodotorula.
Claims (10)
1. a pig's feed additive, its composed as follows according to parts by weight: complex polysaccharide extract 8-15, ocean rhodotorula fermentation culture medium 8-20, Shorthorned Epimedium P.E 5-10, Radix Glycyrrhizae 3-8, hawthorn 3-8, honeysuckle 1-5, dandelion 1-5; Described complex polysaccharide extract is from the Radix Astragali, matrimony vine and clover; Described ocean rhodotorula deposit number is CCTCCNO:M2014592;
Described ocean rhodotorula fermentation culture medium is prepared by the following method: fermentation medium consists of glucose 1%, enzymolysis corn flour 1-3%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 150-200mg/L, sodium chloride 20%, all the other are water; Initial pH is 8, inoculum concentration 3-8%, and shaking speed is 50-80r/min, 24-28 DEG C and cultivates and to adjust temperature after 10-15 hour and be 16-20 DEG C and carry out low temperature static gas wave refrigerator, and stop stirring, pH is adjusted to 3-5, keeps 2-4 hour; Stairstepping is warmed up to 26-30 DEG C afterwards, and pH is adjusted to 4.6-5.5, and add peptone according to the addition of initial medium 2.5%, the amount according to 2% adds dusty yeast, and shaking speed is 50-100r/min, continues fermentation 10-15h; Namely zymotic fluid drying and crushing obtains marine yeast fermentation culture medium after crossing 50-60 mesh sieve.
2. a kind of pig's feed additive as claimed in claim 1, it is characterized in that, the preparation method of described enzymolysis corn flour is: mixed with water by corn flour, and wet pulverizing prepares trickle corn flour feed liquid, add AMS and calcium chloride, regulate pH5.5-6.0,72 DEG C of insulation 15min, then be heated to 90 DEG C of enzymolysis 20-30min, then 55-60 DEG C is cooled to, regulate pH4.5-5.0, add acid protease hydrolysis 30-40min, after enzymolysis liquid centrifuge washing, namely obtain concentrated enzymolysis corn flour.
3. a kind of pig's feed additive as claimed in claim 1, is characterized in that, adds the methionine chelate copper of initial medium weight 3-5wt% in the low temperature static gas wave refrigerator stage described in ocean rhodotorula in culture medium.
4. a kind of pig's feed additive as claimed in claim 1, it is characterized in that, the preparation method of described Shorthorned Epimedium P.E is as follows: after barrenwort pulverized 40-50 mesh sieve, add 50% ethanolic solution of barrenwort 8-10 times of weight, 2 times are extracted with 40-50kHz ultrasonic assistant under room temperature condition, each 30min, filter to obtain ethanol extract, 5-8 times of weight hot water is added in residue after extraction, 80 DEG C decoct 1h, after filtering, solution mixes with ethanol extract, straight fire heating concentrated extracting solution, conventional method is dry, pulverized 60 mesh sieves, obtain Shorthorned Epimedium P.E.
5. a kind of pig's feed additive as claimed in claim 1, it is characterized in that, the preparation method of described complex polysaccharide extract is as follows: the raw material Radix Astragali, alfalfa and matrimony vine drying and crushing are crossed 60 mesh sieves, add crushed material gross weight 5-8 times of boiling water, lixiviate 2-3 each 20-40min, filter and leaching liquor is mixed, naturally cool to room temperature, absolute ethyl alcohol is added in leaching liquor, concentration of alcohol in mixed liquor is made to reach 70-85%, 10 DEG C of airtight refrigeration precipitation 6-8 hour, sediment vacuum drying, pulverize 60 mesh sieves, obtain complex polysaccharide extract.
6. a kind of pig's feed additive as claimed in claim 5, is characterized in that, the mass ratio of the described raw material Radix Astragali, matrimony vine and clover is 1-15:1-15:1-5.
7. a kind of pig's feed additive as claimed in claim 6, is characterized in that, the mass ratio of the described raw material Radix Astragali, matrimony vine and clover is 1-12:1-6:1-3.
8. a kind of pig's feed additive as claimed in claim 1, it is characterized in that, the parts by weight of described feed addictive composed as follows: complex polysaccharide extract 10-12, ocean rhodotorula fermentation culture medium 10-15, Shorthorned Epimedium P.E 6-8, Radix Glycyrrhizae 4-6, hawthorn 4-6, honeysuckle 2-3, dandelion 2-3.
9. a kind of pig's feed additive as claimed in claim 1, it is characterized in that, the parts by weight of described feed addictive are composed as follows: complex polysaccharide extract 10, ocean rhodotorula fermentation culture medium 15, Shorthorned Epimedium P.E 6, Radix Glycyrrhizae 5, hawthorn 5, honeysuckle 2, dandelion 3.
10. the preparation method of pig's feed additive described in claim 1-9, by hawthorn, Radix Glycyrrhizae, honeysuckle and dandelion respectively drying and crushing cross 60 mesh sieves, then to mix in proportion with obtained respectively ocean rhodotorula fermentation culture medium, complex polysaccharide extract and Shorthorned Epimedium P.E.
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| CN110301573A (en) * | 2019-08-06 | 2019-10-08 | 吉林农业大学 | A kind of feedstuff mildew water-preserving promoting agent and preparation method thereof |
| CN111374231A (en) * | 2020-03-20 | 2020-07-07 | 宁乡邦士富养殖有限公司 | Feed for improving meat quality characteristics of pigs and preparation method thereof |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110301573A (en) * | 2019-08-06 | 2019-10-08 | 吉林农业大学 | A kind of feedstuff mildew water-preserving promoting agent and preparation method thereof |
| CN111374231A (en) * | 2020-03-20 | 2020-07-07 | 宁乡邦士富养殖有限公司 | Feed for improving meat quality characteristics of pigs and preparation method thereof |
| CN114052116A (en) * | 2022-01-18 | 2022-02-18 | 山东健源生物科技有限公司 | Probiotic plant extract compound biological agent and preparation method and application thereof |
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