CN1788572A - Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract - Google Patents
Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract Download PDFInfo
- Publication number
- CN1788572A CN1788572A CN 200510111218 CN200510111218A CN1788572A CN 1788572 A CN1788572 A CN 1788572A CN 200510111218 CN200510111218 CN 200510111218 CN 200510111218 A CN200510111218 A CN 200510111218A CN 1788572 A CN1788572 A CN 1788572A
- Authority
- CN
- China
- Prior art keywords
- extract
- humid euphorbia
- powdery mildew
- aqua
- radix seu
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention belongs to the field of farm chemical technology, and is preparation process of gourd powdery mildew controlling water preparation with humid euphorbia extract. The gourd powdery mildew controlling water preparation includes humid euphorbia extract 0.5-5 wt% except water. The preparation process includes the following steps: crushing dried humid euphorbia, room temperature extracting via adding alcohol in 5-20 times and filtering to obtain alcohol extract liquid of humid euphorbia; decompression concentrating the extract liquid to obtain humid euphorbia extractum; and adding distilled water in the same weight as the humid euphorbia powder, ultrasonic dissolution, extracting with n-butanol in the same volume, taking the lower layer of water phase liquid, concentration to dry and obtain humid euphorbia extract, and dissolving in distilled water to obtain the water preparation. The present invention has the features of low cost, effective extraction, no environmental pollution and no residue.
Description
Technical field
That the present invention relates to is a kind of preparation method of technical field of pesticide, specifically is a kind of method for preparing control cucurbits powdery mildew aqua with Radix seu Caulis Parthenocissi tricuspidatae extract.
Background technology
Cucurbits powdery mildew is the melon disease that generally takes place in protection ground.It is fallen ill early, and weight losses is even more serious with the booth melon growth intermediary and later stages especially.At present, the medicament that is used to prevent and treat cucurbits powdery mildew mainly is sterol inhibitor (EBIs), methoxy acrylic (β-methoxyacrylates), phenoxyquinolines class etc.But, because white powder germ reproduction rate height, and an epidemic season can be bred many generations, therefore its germ colony quantity is surprising, and heterogeneous high, systemic in addition bactericide all is the unit point mode of action, so germ very easily develops immunity to drugs under the selection pressure of medicament, and resistance level rises fast.And chemical pesticide has become common recognition to environment, problems such as harm that human health caused.The goal of the invention of this patent promptly utilizes the aqua of China's Chinese herbal medicine Radix seu Caulis Parthenocissi tricuspidatae extract preparation to control the generation of this disease, and for reducing the use of chemical pesticide, production green or organic vegetable provide technical basis.Humid euphorbia (Euphorbia humifusa Willd.) is an euphorbia plant, the annual herbelet of crawling.Have effect clearing heat and detoxicating, cooling blood and hemostasis, be usually used in treating dysentery, enteritis, hemoptysis, have blood in stool, uterine bleeding, sore furuncle carbuncle be swollen.Its water decoction of clinical report, tincture have better curative effect to dysentery, enteritis, and its effect is better than berberine, and avirulence.Pharmacological research shows that its preparation has obvious suppression effect and bactericidal action to multiple pathogenic coccus, bacillus.The chemical composition of bibliographical information humid euphorbia is mainly flavones, sterol and tannin compounds.
Through the prior art literature search is found, Wang Wen and, Xu Yufeng " Beijing Agricultural College's journal " (2004 the 19th the volume, the 1st phase) is entitled as in the article of " Beijing area pesticide plant resource preliminary study " and reported Radix seu Caulis Parthenocissi tricuspidatae extract, corn weevil is had the population of inhibition formation effect as insecticide.But also there is not Radix seu Caulis Parthenocissi tricuspidatae extract to be used to prevent and treat the research report of powdery mildew of cucumber as bactericide at present.Also find in the retrieval, Chinese patent publication number CN1401247, patent name " a kind of Chinese herbal and crude drugs preparations for the treatment of plant powdery mildew and preparation method thereof ", this patent is mentioned the Chinese herbal and crude drugs preparations of the treatment plant powdery mildew that is made of golden cypress, kuh-seng, Chinese prickly ash, Sculellaria barbata, oldenlandia diffusa and dandelion, its preparation method is a decocting method, cost of manufacture is higher relatively, and extraction efficiency is low.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of and prepare the method for controlling the cucurbits powdery mildew aqua, make a kind of humid euphorbia plant of its need can prepare the preparation that is used to prevent and treat cucurbits powdery mildew, and effect is remarkable with Radix seu Caulis Parthenocissi tricuspidatae extract.In addition, easy cultivation of humid euphorbia plant and cost are low, and the relative decocting method of extracting method is more efficient.Aqua of the present invention can be directly used in this disease of control, and has nuisanceless, pollution-free and do not have the characteristics of residual hazard, has avoided the harm of chemical pesticide.
The present invention is achieved by the following technical solutions, and described control cucurbits powdery mildew aqua component and percentage by weight are: Radix seu Caulis Parthenocissi tricuspidatae extract 0.5-5%, and surplus is a water, method step is as follows:
(1) the humid euphorbia herb with drying is ground into 10-50 purpose plant powder with medicinal herb grinder.Add 5-20 doubly to the ethanol of the 25%-100% of humid euphorbia powder weight as extracting solvent, room temperature is extracted, and obtains the humid euphorbia ethanol extract after the filtration.
(2) with the method for humid euphorbia ethanol extract, it is concentrated into paste, gets humid euphorbia and extract medicinal extract with concentrating under reduced pressure.
(3) humid euphorbia is extracted in the medicinal extract and adds and the equal distilled water of humid euphorbia powder weight, places ultrasonic concussion dissolving; Use isopyknic extracting n-butyl alcohol 6 times again; Get lower floor's water liquid after the extraction and be evaporated to driedly, promptly get Radix seu Caulis Parthenocissi tricuspidatae extract, get aqua with dissolved in distilled water.
Described room temperature is extracted, and is meant: the solidliquid mixture of humid euphorbia powder and ethanol is placed under 25 ℃ of conditions, left standstill 24 hours after fully stirring, extract 2-4 time.
Described concentrating under reduced pressure is meant: extract is vacuumized-0.1 atmospheric pressure that reduces pressure, place 45 ℃ of following rotary water baths, extract is concentrated.
Following advantage is compared with prior art arranged: the aqua that the present invention makes derives from the extract of Chinese herbal medicine humid euphorbia, utilize the water-soluble characteristics of active component, remove impurity with organic solvent extractionprocess, be mixed with aqua, environmentally safe, to people, animal safety, be a kind of pollution-free plant source biological bactericide; The aqua that the present invention makes is that wide to distribute, resourceful humid euphorbia is a raw material, is easy to artificial cultivation, and extraction process is simple, need not special instrument, and the solvent after the recovery also can reuse; The aqua that the present invention makes has fabulous control efficiency to cucurbits powdery mildew.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment:
Embodiment 1:
Control cucurbits powdery mildew aqua percentage by weight: Radix seu Caulis Parthenocissi tricuspidatae extract 0.5%, distilled water 99.5%.
Take by weighing humid euphorbia powder (10 order particle) 100g, extract 2 times with 25% ethanol 500ml room temperature, each 24 hours, concentrating under reduced pressure reclaims solvent must extract medicinal extract 7.4g; Utilize ultrasonic, will extract medicinal extract concussion dissolving, use equal-volume extracting n-butyl alcohol 6 times with 100ml distilled water, lower floor's water liquid of getting after the extraction is evaporated to dried, obtain extract 5.5g, filter, be mixed with concentration and be 0.5% aqua with 1100ml dissolved in distilled water extract; Adopt the leaf disc method to the cucurbits powdery mildew test of pesticide effectiveness that exsomatizes, preventive effect reaches 76.93%.
Embodiment 2:
Control cucurbits powdery mildew aqua percentage by weight: Radix seu Caulis Parthenocissi tricuspidatae extract 2%, distilled water 98%.
Take by weighing humid euphorbia powder (30 order particle) 100g, extract 3 times with 75% ethanol 1000ml room temperature, each 24 hours, concentrating under reduced pressure reclaims solvent must extract medicinal extract 8.4g; Utilize ultrasonic, will extract medicinal extract concussion dissolving, use equal-volume extracting n-butyl alcohol 6 times with 100ml distilled water, lower floor's water liquid of getting after the extraction is evaporated to dried, obtain extract 6.2g, filter, be mixed with concentration and be 2% aqua with 310ml dissolved in distilled water extract; Adopt the indoor pot experiment that cucurbits powdery mildew is carried out the live body test of pesticide effectiveness, preventive effect reaches 68.5%.
Embodiment 3:
Control cucurbits powdery mildew aqua percentage by weight: Radix seu Caulis Parthenocissi tricuspidatae extract 5%, distilled water 95%.
Take by weighing humid euphorbia powder (50 order particle) 100g, extract 4 times with 100% ethanol 2000ml room temperature, each 24 hours, concentrating under reduced pressure reclaims solvent must extract medicinal extract 9.6g; Utilize ultrasonic, will extract medicinal extract concussion dissolving, use equal-volume extracting n-butyl alcohol 6 times with 100ml distilled water, lower floor's water liquid of getting after the extraction is evaporated to dried, obtain extract 7.0g, filter, be mixed with concentration and be 5% aqua with 140ml dissolved in distilled water extract; Adopt field plot trial that cucurbits powdery mildew is carried out the test of pesticide effectiveness, its preventive effect reaches 47.35%.
Claims (6)
1, a kind ofly prepare the method for control cucurbits powdery mildew aqua, it is characterized in that described control cucurbits powdery mildew aqua component and percentage by weight are with Radix seu Caulis Parthenocissi tricuspidatae extract: Radix seu Caulis Parthenocissi tricuspidatae extract 0.5-5%, surplus is a water, method step is as follows:
(1) the humid euphorbia herb of drying is pulverized, add 5-20 doubly to humid euphorbia powder weight ethanol as extracting solvent, room temperature is extracted, and obtains the humid euphorbia ethanol extract after the filtration;
(2) with the method for humid euphorbia ethanol extract, it is concentrated into paste, gets humid euphorbia and extract medicinal extract with concentrating under reduced pressure;
(3) humid euphorbia is extracted in the medicinal extract and adds and the equal distilled water of humid euphorbia powder weight, place ultrasonic concussion dissolving, use isopyknic extracting n-butyl alcohol again, lower floor's water liquid of getting after the extraction is evaporated to dried, promptly get Radix seu Caulis Parthenocissi tricuspidatae extract, get aqua with dissolved in distilled water.
2, according to claim 1ly prepare the method for control cucurbits powdery mildew aqua, it is characterized in that described humid euphorbia herb with drying is pulverized, and is meant: be ground into 10-50 purpose plant powder with medicinal herb grinder with Radix seu Caulis Parthenocissi tricuspidatae extract.
3, according to claim 1ly prepare the method for control cucurbits powdery mildew aqua, it is characterized in that with Radix seu Caulis Parthenocissi tricuspidatae extract, described ethanol, its concentration is 25%-100%.
4, the method for preparing control cucurbits powdery mildew aqua with Radix seu Caulis Parthenocissi tricuspidatae extract according to claim 1, it is characterized in that described room temperature is extracted, and is meant: the solidliquid mixture of humid euphorbia powder and ethanol is placed under 25 ℃ of conditions, left standstill 24 hours after fully stirring, extract 2-4 time.
5, the method for preparing control cucurbits powdery mildew aqua with Radix seu Caulis Parthenocissi tricuspidatae extract according to claim 1, it is characterized in that described concentrating under reduced pressure is meant: extract is vacuumized-0.1 atmospheric pressure that reduces pressure, place 45 ℃ of following rotary water baths, extract is concentrated.
6, according to claim 1ly prepare the method for control cucurbits powdery mildew aqua, it is characterized in that, with isopyknic extracting n-butyl alcohol 6 times with Radix seu Caulis Parthenocissi tricuspidatae extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510111218 CN1788572A (en) | 2005-12-08 | 2005-12-08 | Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510111218 CN1788572A (en) | 2005-12-08 | 2005-12-08 | Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1788572A true CN1788572A (en) | 2006-06-21 |
Family
ID=36786850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510111218 Pending CN1788572A (en) | 2005-12-08 | 2005-12-08 | Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1788572A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715198A (en) * | 2012-06-15 | 2012-10-10 | 米如钦 | Traditional Chinese herbal medicine mixture for preventing and controlling cucumber bacterial angular leaf spot |
| CN103583589A (en) * | 2013-11-27 | 2014-02-19 | 上海海洋大学 | Karenia mikimotoi inhibitor, preparation method and application thereof |
| CN104351263A (en) * | 2014-11-03 | 2015-02-18 | 上海海洋大学 | Water bloom alga seed suppressor and preparation method and application thereof |
| CN104920494A (en) * | 2015-06-04 | 2015-09-23 | 胡凡营 | Biopesticide for controlling tomato powdery mildew and preparation method of biopesticide |
-
2005
- 2005-12-08 CN CN 200510111218 patent/CN1788572A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715198A (en) * | 2012-06-15 | 2012-10-10 | 米如钦 | Traditional Chinese herbal medicine mixture for preventing and controlling cucumber bacterial angular leaf spot |
| CN102715198B (en) * | 2012-06-15 | 2013-07-03 | 米如钦 | Traditional Chinese herbal medicine mixture for preventing and controlling cucumber bacterial angular leaf spot |
| CN103583589A (en) * | 2013-11-27 | 2014-02-19 | 上海海洋大学 | Karenia mikimotoi inhibitor, preparation method and application thereof |
| CN103583589B (en) * | 2013-11-27 | 2015-04-22 | 上海海洋大学 | Application methof of karenia mikimotoi inhibitor |
| CN104351263A (en) * | 2014-11-03 | 2015-02-18 | 上海海洋大学 | Water bloom alga seed suppressor and preparation method and application thereof |
| CN104920494A (en) * | 2015-06-04 | 2015-09-23 | 胡凡营 | Biopesticide for controlling tomato powdery mildew and preparation method of biopesticide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105379771A (en) | Plant-derived pesticide and its preparation method and use | |
| CN105532755A (en) | Novel plant source insecticide for fruit trees, preparation method and application thereof | |
| CN103430972B (en) | Method for preparing biological bactericide for fruit trees by utilizing propolis and beewax | |
| CN105519612A (en) | Botanical insecticide for fruit trees, preparation method and application thereof | |
| CN105594765A (en) | Environment-friendly pesticide | |
| CN105125438A (en) | Mosquito-repellent composition and mosquito-repellent liquid preparation method | |
| CN105061616A (en) | Tetrastlgma hemsleyanum vine polysaccharide extract, preparation method and application thereof | |
| Dwivedi | Anthelmintic activity of alcoholic and aqueous extract of fruits of Terminalia chebula Retz. | |
| CN103609597A (en) | Botanical synergistic compound pesticide | |
| CN100486441C (en) | Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew | |
| CN1788572A (en) | Method for preparing aqueous formulation for controlling melon powdery mildew using prostrate spurge extract | |
| CN111000927A (en) | Antibacterial lotion for female vulva and preparation method thereof | |
| CN110721095A (en) | Propolis-containing herbal oral bacteriostatic spray and preparation method thereof | |
| CN102218026B (en) | Use of willow leaf wintersweet extract in preparation of mosquito repelling agent | |
| CN112244042B (en) | Application of herba delphinii or extract thereof in preventing and treating sisalana ananas comosus | |
| CN108785363A (en) | Application of the yellow wingceltis platymiscium perfume (or spice) material in the drug for preparing treatment microorganism infection | |
| CN108553572A (en) | A kind of functional incense and preparation method thereof | |
| CN104695034B (en) | Traditional Chinese medicine mosquito expelling composition and application thereof | |
| CN107998025B (en) | Pepper essential oil mosquito-repellent floral water and preparation method thereof | |
| CN105770099A (en) | Externally applied traditional Chinese medicine for treating tinea corporis and cruris and tinea manus and pedis | |
| CN106511471B (en) | A kind of fig oral spray and preparation method thereof | |
| CN106491374B (en) | Nepetalactone microcapsule, preparation method thereof and application of nepetalactone microcapsule in preparation of mosquito repellent product | |
| CN110965320A (en) | Preparation process of negative oxygen ion carpet | |
| CN108619365A (en) | A kind of medical thimerosal and preparation method thereof | |
| CN101862392B (en) | New pharmaceutical application of cordate houttuynia in thrombus prevention |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |