CN108619365A - A kind of medical thimerosal and preparation method thereof - Google Patents
A kind of medical thimerosal and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of medical thimerosals and preparation method thereof.Include the raw material of following parts by weight：38 parts of dandelion extract, 6 10 parts of garlic P.E, 36 parts of Herba Kummerowiae Striatae extract, 38 parts of ophiopogonin, 38 parts of tea polyphenols, 38 parts of terpinol, 150 250 parts of water.Medical thimerosal prepared by the present invention, bactericidal effect is good, can be used for the cleaning and sterilizing of medical instrument and wound, is used for the cleaning and sterilizing of wound, also has effects that relieve pain and promote wound healing.The active Fungicidal substance dandelion extract that the present invention uses, garlic P.E and Herba Kummerowiae Striatae extract are derived from natural plants, environmental sound, and liquid waste is easy in nature fast degradation.
Description
Technical field
The invention belongs to medical material preparing technical fields, and in particular to a kind of medical thimerosal and its preparation side
Method.
Background technology
Currently, cleaning a wound in hospital, injection needle takes blood, operation etc. to be required for carrying out disinfection using thimerosal, disinfection
For purpose primarily to prevent wound infection, the disinfection of general wound generally uses the tincture of iodine, and medicinal alcohol carries out disinfection, and notes
Penetrate needle and take and generally use medicinal alcohol cotton cleaning disinfection before blood, it is traumatic using physiological saline clean compared with gash disappear
Poison.Current thimerosal the problem is that disinfection is not thorough, in sterilization process traumatic patient pain unbearably, and wound
Healing is slower.And above-mentioned chemosterilant there are environmental pollution, medicament residue, develop immunity to drugs the problems such as, so far still without one kind
Sterilizing chemical products can reach the standard of preferred disinfectants.
With disinfection technology and horizontal continuous development, the plant source sterilization of environmentally protective, wide sterilization spectrum and high-efficiency low-toxicity
Disinfectant is just gradually paid attention to by more and more people, and plant is considered as the preferably exploitation money of chemical classes disinfectant substitute
Source.Mendez etc. is by studying American aloe, cactus etc., as a result, it has been found that their extract can inhibit large intestine bar
The growth of bacterium, staphylococcus aureus and salmonella typhi etc.；Li et al. research finds champignon essence oil to aspergillus oryzae and golden yellow
Staphylococcus has very strong inhibiting effect, and then weaker to the inhibition of Escherichia coli；Burnt radium etc. has studied 15 kinds of Chinese herbal medicines
And its compositional liquor is to the inhibiting effect of Escherichia coli, bacillus subtilis, staphylococcus aureus and Candida, as a result, it has been found that
The extracting solution of 5 kinds of plants such as cloves, kuh-seng has good bacteriostasis property；Wang Qianwen etc. reports 89 kinds of medium-height grass such as Radix Salviae Miltiorrhizae, rhizoma anemarrhenae
Medicine alcohol extract is to yeast, Escherichia coli and the antibacterial effect of staphylococcus aureus；Hu Meijiao etc. determines 41 section, 72 kinds of medium-height grass
The mycelial growth inhibition situation of 9 kinds of germs of medicament extract pair, the results showed that, Flos Caryophylli extract is to all tested germ mycelium
Inhibiting rate all reached 100%.
Patent 201210029524.4 discloses a kind of thimerosal.It is prepared by the bulk pharmaceutical chemicals of following parts by weight：Kuh-seng
It is 5g, coptis 5g, Cortex Phellodendri 5g, tuber of stemona 5g, smilax 5g, fruit of glossy privet 10g, folium artemisiae argyi 5g, fruit of Rangoon creeper 5g, fruit of summer cypress 5g, cloves 3g, sweet
Careless 3g.Although the thimerosal active constituent is all made of Chinese medicine material, the work of tween surface is added in preparation method
Property agent, being irritating to the skin property is strong for the feeling of pain that cleans a wound, and be not easy promote wound healing.
Invention content
The purpose of the present invention is to provide a kind of medical thimerosals, and while efficiently killing bacterium, mitigation cleans a wound
When pain, promote wound healing.
The present invention also aims to provide the preparation method of above-mentioned medical thimerosal.
A kind of medical thimerosal, includes the raw material of following parts by weight：3-8 parts of dandelion extract, garlic P.E
6-10 parts, 3-6 parts of Herba Kummerowiae Striatae extract, 3-8 parts of ophiopogonin, 3-8 parts of tea polyphenols, 3-8 parts of terpinol, 150-250 parts of water.
The ophiopogonin is ophiopogonin B, ophiopogonin D, the mixture of ophiopogonin F or its arbitrary component.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 30-50 mesh sieve is crossed；It is added
75% ethanol solution, microwave power 400-600w is arranged in Microwave Extraction Apparatus, and extraction time 40-60s is extracted, carried
Take liquid；The above extracting solution is concentrated and obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 4 times, acetone extract liquid is obtained, through revolving
Dandelion extract is obtained after removing solvent.
The extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 15-20 times of parts by weight, set
It extracts, extracts 3 times, each extraction time 1-3h altogether, 60-70 DEG C of Extracting temperature on water bath with thermostatic control oscillator；It has extracted
It is filtered after finishing, collects filtrate, filtrate is concentrated under the conditions of 45-55 DEG C, 0.06-0.10Mpa, concentration volume to original volume
1/ (12-18), garlic P.E is made.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 40-60 mesh sieve is crossed；It is added
70% ethanol solution, microwave power 400-600w is arranged in Microwave Extraction Apparatus, and extraction time 40-60s is extracted, carried
Take liquid；The above extracting solution is concentrated and obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, through revolving
Herba Kummerowiae Striatae extract is obtained after removing solvent.
It further includes that whip branch hangs 3-8 parts of moss extract that the thimerosal, which is prepared in raw material,.
The extracting method that the whip branch hangs moss extract is：Whip branch is hanged moss to dry, is ground into powder, crosses 60-80 mesh sieve,
Add the 70% ethanol water refluxing extraction 3 times of 10-15 times of parts by weight, every time extraction 1-3 hours, merging filtrate, activated carbon
Decoloration, is filtered to remove activated carbon, is evaporated filtrate, be made.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) it according to parts by weight, fetches water 150-250 parts, is heated to 55-65 DEG C, 3-8 parts of ophiopogonin, tea polyphenols are added
3-8 parts, 3-8 parts of terpinol stirs 10-30min, fully dissolves, be cooled to room temperature；
(2) 3-8 parts of dandelion extract, 6-10 parts of garlic P.E is added, 3-6 parts of Herba Kummerowiae Striatae extract stirs 10-
30min is made.
It is additionally added whip branch in step (2) and hangs 3-8 parts of moss extract.
Beneficial effects of the present invention：Medical thimerosal prepared by the present invention, bactericidal effect is good, can be used for medical instrument
And the cleaning and sterilizing of wound, it is used for the cleaning and sterilizing of wound, also has effects that relieve pain and promote wound healing.The present invention uses
Active Fungicidal substance dandelion extract, garlic P.E and Herba Kummerowiae Striatae extract be derived from natural plants, and environmental sound gives up
Liquid is abandoned to be easy in nature fast degradation.
Specific implementation mode
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 6g, garlic P.E 8g,
Herba Kummerowiae Striatae extract 5g, ophiopogonin 5g, tea polyphenols 5g, terpinol 6g, water 200g.The ophiopogonin is ophiopogonin B, wheat
Winter saponin D, ophiopogonin is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 40 mesh sieve is crossed；It is added 75%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition with third
Ketone extracts 4 times, obtains acetone extract liquid, and dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 6g, garlic P.E 8g, Herba Kummerowiae Striatae extract 5g is added, stirs 20min, is made.
Embodiment 2
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 3g, garlic P.E 6g,
Herba Kummerowiae Striatae extract 3g, ophiopogonin B 3g, tea polyphenols 3g, terpinol 3g, water 150g.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 30 mesh sieve is crossed；It is added 75%
Microwave power 400w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 40s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and the extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition
With acetone extract 4 times, acetone extract liquid is obtained, dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 15 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 1h altogether, 60 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.06Mpa by filtrate at 45 DEG C, and the 1/12 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 40 mesh sieve is crossed；It is added 70%
Microwave power 400w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 40s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 150g, is heated to 55 DEG C, and ophiopogonin 3g, tea polyphenols 3g, terpinol 3g is added,
10min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 3g, garlic P.E 6g, Herba Kummerowiae Striatae extract 3g is added, stirs 10min, is made.
Embodiment 3
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 8g, garlic P.E 10g,
Herba Kummerowiae Striatae extract 6g, ophiopogonin D 8g, tea polyphenols 8g, terpinol 8g, water 250g.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 50 mesh sieve is crossed；It is added 75%
Microwave power 600w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 60s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and the extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition
With acetone extract 4 times, acetone extract liquid is obtained, dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 20 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 3h altogether, 70 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.10Mpa by filtrate at 55 DEG C, and the 1/18 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 60 mesh sieve is crossed；It is added 70%
Microwave power 600w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 60s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 250g, is heated to 65 DEG C, and ophiopogonin 8g, tea polyphenols 8g, terpinol 8g is added,
30min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 8g, garlic P.E 10g, Herba Kummerowiae Striatae extract 6g is added, stirs 30min, is made.
Embodiment 4
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 6g, garlic P.E 8g,
Herba Kummerowiae Striatae extract 5g, ophiopogonin 5g, whip branch hang moss extract 5g, tea polyphenols 5g, terpinol 6g, water 200g.The Radix Ophiopogonis
Saponin(e is ophiopogonin B, and ophiopogonin D, ophiopogonin is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method that the whip branch hangs moss extract is：Whip branch is hanged into moss (Barbella flagellifera
(card.) nog.) it dries, it is ground into powder, 70 mesh sieve is crossed, adds 70% ethanol water refluxing extraction 3 of 12 times of parts by weight
It is secondary, it extracts 2 hours every time, merging filtrate, activated carbon decolorizing is filtered to remove activated carbon, is evaporated filtrate, is made.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 40 mesh sieve is crossed；It is added 75%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition with third
Ketone extracts 4 times, obtains acetone extract liquid, and dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 6g, garlic P.E 8g, Herba Kummerowiae Striatae extract 5g is added, whip branch hangs moss extract 5g, stirs
20min is mixed, is made.
Embodiment 5
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 6g, garlic P.E 8g,
Herba Kummerowiae Striatae extract 5g, ophiopogonin 5g, outstanding greatly moss extract 5g, tea polyphenols 5g, terpinol 6g, water 200g.The Radix Ophiopogonis soap
Glycosides is ophiopogonin B, and ophiopogonin D, ophiopogonin is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method of the big outstanding moss extract is：Moss (Barbella asperifolia card.) solarization will be hanged greatly
It is dry, it is ground into powder, crosses 70 mesh sieve, adds the 70% ethanol water refluxing extraction 3 times of 12 times of parts by weight, extraction 2 is small every time
When, merging filtrate, activated carbon decolorizing is filtered to remove activated carbon, is evaporated filtrate, is made.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 40 mesh sieve is crossed；It is added 75%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition with third
Ketone extracts 4 times, obtains acetone extract liquid, and dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 6g, garlic P.E 8g, Herba Kummerowiae Striatae extract 5g is added, moss extract 5g outstanding greatly, stirring
20min is made.
Embodiment 6
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 6g, garlic P.E 8g,
Herba Kummerowiae Striatae extract 5g, hair breast chrysanthemum extract 5g, ophiopogonin 5g, tea polyphenols 5g, terpinol 6g, water 200g.The Radix Ophiopogonis soap
Glycosides is ophiopogonin B, and ophiopogonin D, ophiopogonin is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method of the hair breast chrysanthemum extract is：By hair breast chrysanthemum (Galactites tomentosa Moench) flower
It piece dries, crushes, cross 40 mesh sieve；75% ethanol solution is added, microwave power 500w, extraction time are set in Microwave Extraction Apparatus
50s is extracted, and obtains extracting solution；The above extracting solution is concentrated and obtains medicinal extract, extracting solution concentration is concentrated under reduced pressure at 45 DEG C
To 1/6 volume of stoste；By the medicinal extract of acquisition with acetone extract 4 times, acetone extract liquid is obtained, hair is obtained after revolving removes solvent
Newborn chrysanthemum extract.
The extracting method of the dandelion extract is：Dandelion herb is dried, is crushed, 40 mesh sieve is crossed；It is added 75%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract, and extracting solution concentration is that 1/6 volume of stoste is concentrated under reduced pressure at 45 DEG C；By the medicinal extract of acquisition with third
Ketone extracts 4 times, obtains acetone extract liquid, and dandelion extract is obtained after revolving removes solvent.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 6g, garlic P.E 8g, Herba Kummerowiae Striatae extract 5g, hair breast chrysanthemum extract 5g, stirring is added
20min is made.
Embodiment 7
A kind of medical thimerosal, includes the raw material of following parts by weight：Dandelion extract 6g, garlic P.E 8g,
Herba Kummerowiae Striatae extract 5g, ophiopogonin 5g, tea polyphenols 5g, terpinol 6g, water 200g.The ophiopogonin is ophiopogonin B, wheat
Winter saponin D, ophiopogonin is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method of the dandelion extract is：After fresh dandelion herb is smashed to pieces, add 18 times of parts by weight
Water is placed on water bath with thermostatic control oscillator and extracts, and extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature；It has extracted
It is filtered after finishing, collects filtrate and concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, the 1/15 of concentration volume to original volume,
Dandelion extract is made.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) dandelion extract 6g, garlic P.E 8g, Herba Kummerowiae Striatae extract 5g is added, stirs 20min, is made.
Embodiment 8
A kind of medical thimerosal, includes the raw material of following parts by weight：Garlic P.E 8g, Herba Kummerowiae Striatae extract 5g,
Ophiopogonin 5g, tea polyphenols 5g, terpinol 6g, water 200g.The ophiopogonin is ophiopogonin B, ophiopogonin D, Radix Ophiopogonis soap
Glycosides is according to mass ratio 1：1：The mixture of 1 proportioning.
The extracting method of the garlic P.E is：After fresh garlic is smashed to pieces, adds the water of 18 times of parts by weight, be placed in perseverance
It extracts, extracts 3 times, each extraction time 2h altogether, 65 DEG C of Extracting temperature on tepidarium oscillator；It is taken out after extraction
Filter is collected filtrate and is concentrated under the conditions of 0.08Mpa by filtrate at 50 DEG C, and the 1/15 of concentration volume to original volume, garlic is made and carries
Take object.
The extracting method of the Herba Kummerowiae Striatae extract is：Clover herb is dried, is crushed, 50 mesh sieve is crossed；It is added 70%
Microwave power 500w is arranged in Microwave Extraction Apparatus for ethanol solution, and extraction time 50s is extracted, and obtains extracting solution；Will more than
Extracting solution concentration obtains medicinal extract；By the medicinal extract of acquisition with acetone extract 3 times, acetone extract liquid is obtained, after revolving removes solvent
Obtain Herba Kummerowiae Striatae extract.
The preparation method of above-mentioned medical thimerosal, includes the following steps：
(1) according to parts by weight, fetch water 200g, is heated to 60 DEG C, and ophiopogonin 5g, tea polyphenols 5g, terpinol 6g is added,
20min is stirred, fully dissolves, is cooled to room temperature；
(2) garlic P.E 8g, Herba Kummerowiae Striatae extract 5g is added, stirs 20min, is made.
Experimental example 1：
The thimerosal of series concentration is uniformly mixed with agar medium using MH dilution methods, then by 2 μ l bacteria suspensions
(107Cfu/ml it) smears and is inoculated on tablet；Set the control of no medicine blank plate simultaneously, after culture by observe the growth of bacterium with
It is no, to determine minimal inhibitory concentration (MIC) of the drug to tested bacterium.Experimental result is shown in Table 1：
MIC (mg/ml) of the 1 each group thimerosal of table to Escherichia coli and staphylococcus aureus
As can be seen from Table 1, embodiment 1-3, embodiment 5 is identical for the minimal inhibitory concentration of Escherichia coli, embodiment 4
It is far smaller than embodiment 1 with the minimal inhibitory concentration of embodiment 6, and the minimal inhibitory concentration of embodiment 7 and embodiment 8 is much big
In embodiment 1；Embodiment 1-3, embodiment 5 is identical for the minimal inhibitory concentration of staphylococcus aureus, embodiment 4 and implementation
The minimal inhibitory concentration of example 6 is far smaller than embodiment 1, and the minimal inhibitory concentration of embodiment 7 and embodiment 8 is far longer than implementation
Example 1.
Experimental example 2：
Experiment material used in this experiment is SPF grades of C57BL/6 male mices；By Shanghai SLRC experimental animals Limited Liability public affairs
Department provides, and experimental animal production licence number is：SCXK (Shanghai) 2003-0003.
The SPF grade C57BL/6 male mices that selection weight is 20g or so are observed one week, nothing in 23-25 DEG C of daily raising
Dead and insanity occurs.Mouse is randomly divided into 9 groups, every group 10.After skin of back depilation, with penta bar of ratio of 0.5mg/g
Appropriate sodium anesthesia, makes a call to a circular hole at mouse back with card punch, the full thickness skin of a diameter of 10.0mm is cut, with sterile yarn after hemostasis
Cloth is wrapped up, and single cage is fed.
The each group mouse skin surface of a wound carries out cleaning 3 times using thimerosal, and absorbent cotton sticks fixed wound after cleaning, cleans daily
1 time, continuous wash 3 weeks.
It carries out wound and takes pictures within 14 days after wound, analyzed and calculated small with 6.0 image analysis softwares of Image-Pro Plus
The area of mouse skin wound, and mouse wound healing rate is calculated.Healing rate=[(original surface of a wound area-do not heal the surface of a wound
Area) the original surface of a wound areas of ÷] × 100%.Test result is shown in Table shown in 2-3：
2 each experimental group healing rate of table
Note：* P compared with the control group is represented<0.05.
As can be seen from Table 2, the healing rate of embodiment 1-3 is significantly higher than control group, it was demonstrated that the thimerosal of embodiment 1-3 has
There is the effect of certain promotion wound healing.
3 each experimental group healing rate of table
Note：* the P compared with 1 group of embodiment is represented<0.05；**P<0.01.
As can be seen from Table 3, the healing rate of embodiment 4 is significantly higher than embodiment 1, healing rate and the embodiment 1 of embodiment 5
It is not significantly different, the healing rate pole of embodiment 6 is significantly higher than embodiment 1, and the healing rate of embodiment 7 and embodiment 8 is significantly low
In embodiment 1.
Claims (10)
1. a kind of medical thimerosal, which is characterized in that include the raw material of following parts by weight：3-8 parts of dandelion extract, greatly
6-10 parts of garlic extract, 3-6 parts of Herba Kummerowiae Striatae extract, 3-8 parts of ophiopogonin, 3-8 parts of tea polyphenols, 3-8 parts of terpinol, water 150-
250 parts.
2. medical thimerosal according to claim 1, which is characterized in that the ophiopogonin is ophiopogonin B, Radix Ophiopogonis soap
The mixture of glycosides D, ophiopogonin F or its arbitrary component.
3. medical thimerosal according to claim 1, which is characterized in that the extracting method of the dandelion extract is：
Dandelion herb is dried, is crushed, 30-50 mesh sieve is crossed；75% ethanol solution is added, microwave power is set in Microwave Extraction Apparatus
400-600w, extraction time 40-60s, extracts, and obtains extracting solution；The above extracting solution is concentrated and obtains medicinal extract；By acquisition
Medicinal extract obtains acetone extract liquid with acetone extract 4 times, and dandelion extract is obtained after revolving removes solvent.
4. medical thimerosal according to claim 3, which is characterized in that extracting solution concentration be depressurized at 45 DEG C it is dense
It is reduced to 1/6 volume of stoste.
5. medical thimerosal according to claim 1, which is characterized in that the extracting method of the garlic P.E is：It will
After fresh garlic is smashed to pieces, add the water of 15-20 times of parts by weight, be placed on water bath with thermostatic control oscillator and extract, extracts 3 times altogether,
Each extraction time 1-3h, 60-70 DEG C of Extracting temperature；It is filtered after extraction, collects filtrate, by filtrate at 45-55 DEG C,
It is concentrated under the conditions of 0.06-0.10Mpa, garlic P.E is made in 1/ (12-18) of concentration volume to original volume.
6. medical thimerosal according to claim 1, which is characterized in that the extracting method of the Herba Kummerowiae Striatae extract is：
Clover herb is dried, is crushed, 40-60 mesh sieve is crossed；70% ethanol solution is added, microwave power is set in Microwave Extraction Apparatus
400-600w, extraction time 40-60s, extracts, and obtains extracting solution；The above extracting solution is concentrated and obtains medicinal extract；By acquisition
Medicinal extract obtains acetone extract liquid with acetone extract 3 times, and Herba Kummerowiae Striatae extract is obtained after revolving removes solvent.
7. medical thimerosal according to claim 1, which is characterized in that it further includes whip branch that the thimerosal, which is prepared in raw material,
Outstanding 3-8 parts of moss extract.
8. medical thimerosal according to claim 1, which is characterized in that the whip branch hangs the extracting method of moss extract
For：Whip branch is hanged moss to dry, is ground into powder, crosses 60-80 mesh sieve, 70% ethanol water of 10-15 times of parts by weight is added to return
Stream extraction 3 times, is extracted 1-3 hours, merging filtrate, activated carbon decolorizing is filtered to remove activated carbon, is evaporated filtrate, is made every time.
9. the preparation method of medical thimerosal described in claim 1, which is characterized in that include the following steps：
(1) it according to parts by weight, fetches water 150-250 parts, is heated to 55-65 DEG C, 3-8 parts of ophiopogonin, tea polyphenols 3-8 is added
Part, 3-8 parts of terpinol stirs 10-30min, fully dissolves, be cooled to room temperature；
(2) 3-8 parts of dandelion extract, 6-10 parts of garlic P.E is added, 3-6 parts of Herba Kummerowiae Striatae extract stirs 10-30min,
It is made.
10. the preparation method of medical thimerosal according to claim 1, which is characterized in that step is additionally added whip branch in (2)
Outstanding 3-8 parts of moss extract.
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| CN112957417A (en) * | 2021-03-02 | 2021-06-15 | 上海芝道实业有限公司 | Preparation method of purple garlic skin powder with health preserving effect |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105560763A (en) * | 2016-02-25 | 2016-05-11 | 张宁欣 | Traditional Chinese medicine composition skin disinfectant |
| CN106172515A (en) * | 2016-06-28 | 2016-12-07 | 郑州雷曼药业有限公司 | A kind of disinfectant solution and preparation method thereof |
| CN107308333A (en) * | 2017-06-29 | 2017-11-03 | 贵州松桃信仁苗药有限公司 | It is a kind of to promote Chinese medicine composition of wound healing and preparation method thereof |
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2018
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105560763A (en) * | 2016-02-25 | 2016-05-11 | 张宁欣 | Traditional Chinese medicine composition skin disinfectant |
| CN106172515A (en) * | 2016-06-28 | 2016-12-07 | 郑州雷曼药业有限公司 | A kind of disinfectant solution and preparation method thereof |
| CN107308333A (en) * | 2017-06-29 | 2017-11-03 | 贵州松桃信仁苗药有限公司 | It is a kind of to promote Chinese medicine composition of wound healing and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112957417A (en) * | 2021-03-02 | 2021-06-15 | 上海芝道实业有限公司 | Preparation method of purple garlic skin powder with health preserving effect |
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Application publication date: 20181009 |