Larvae hatching method of adhesive egg
Technical field
The invention belongs to the fish culture technical field, particularly relate to a kind of larvae hatching method of adhesive egg.
Background technology
At present, the fish culture hatching, the traditional hatching pattern of general employing, promptly at the outdoor induced spawning that carries out, natural spawning is on the palm sheet, and circuit is hatched, this hatching pattern is subjected to extraneous water source and climatic effect big, in case weather occurs unusual,, can have a strong impact on the activity of laying eggs, incubation rate and the survival rate of seedling of parent population as cold current etc.The shortcoming of this kind fry hatching mode maximum is that the fish-egg density of natural spawning on the palm sheet is low, and single pond fish seedling yields poorly.Grow seedlings and produce very big restriction in a planned way carrying out commercialization, become the key link that the restriction fry produces.
Summary of the invention
The present invention provides a kind of larvae hatching method of adhesive egg for solving the problem that exists in the known technology.Purpose of the present invention is exactly to improve the hatching output of single pond fish seedling greatly by fish-egg being distributed in the mode that the net sheet is hatched, and improves production capacity.
The present invention includes: the cultivation of parent population, hasten parturition, insemination and hatching process:
1. the cultivation of parent population
Carry out parent fish selection before surviving the winter in the fall, proterties standard, parent population that development degree is good are put into propagating and cultivating pond early.Begin to throw something and feed when early stage water temperature was more than 9 ℃ in 1 year, main feed is maize pulp and Fructus Hordei Germinatus etc.
2. hasten parturition
Reach the suitable water temperature of breeding in water temperature after, promptly can begin to hasten parturition, because the speeding up of the gonadal maturation by the intensification parent population, pass through stimulation by running water again, normal hatching production can be guaranteed,, just hastening parturition can be begun the carp crucian in 1-5 day in the April in every year.
When hastening parturition, select belly and expand softly, the raun of sense of movement and the milter of ripe appropriateness are arranged, put into spawning inducing pool.The dosage of hastening parturition is respectively DOM 2-0.4mg, LRH-A 2-0.4ug, and HCG 1000-800I.U. hastens parturition and preferably takes biphasic injection, and the first injection amount is the 1/5-1/3 of total amount; The disposable injection of milter, dosage are 1/3 of raun, inject for the second time simultaneously raun and carry out, and the temperature of hastening parturition preferably remains on more than 19 ℃.
3. insemination and cloth ovum
After carp crucian parent population reaches the effector phase of laying eggs, fish-egg is clamp-oned in the dried basin, clamp-on seminal fluid then, the jog basin mixes smart ovum.In ovum distributing pool, fertilized egg is evenly distributed in long 80CM, wide 60CM is on the 25 purpose polythene net sheets.Require per kilogram fertilized egg cloth 12-14 sheet net sheet, fertilized egg must be evenly distributed, and caking can not occur, and one deck preferably tiles on the net sheet.In the strict control time in operating process, every basin collection ovum time must not surpass 5 minutes, and weight must not surpass 1 kilogram, and the cloth ovum time must not surpass 2 minutes, so as not to smart ovum when mixing anoxic and fertilized egg bond.Requiring during the cloth ovum has the dark clear water of 10-20CM in the ovum distributing pool, and the net sheet will be convenient to make fertilized egg being evenly distributed about the 5CM of underwater.Net sheet behind the cloth ovum was tiled in the ovum distributing pool 20-30 minute, and fertilized egg is cohered on the net sheet firmly, and by soaking and mucus is eliminated in rinsing, avoided polluting the water quality of hatchery, and the taking-up extension the hatchery and hatched then.
4. hatching
Before carp crucian collection ovum net sheet is gone into the pond,, inflate earlier with filling with clear water in the hatchery.Then cloth ovum net sheet around is suspended on the iron wire of same hatchery, the net sheet is not had in the entry, every pond net sheet fixed amount is about 120.Between the incubation period, should uninterruptedly inflate, keep dissolved oxygen about 7-8mg/l, best hatching water temperature is controlled at about 22 ℃.
5. the collection of spray
Sprays such as carp crucian grow waist point appears and after, can go out the pond.
Larvae hatching method of adhesive egg of the present invention is taked following technical scheme:
Larvae hatching method of adhesive egg, through parent population cultivate, hasten parturition, insemination and hatching process, the fertilized egg after being characterized in inseminating is distributed on the net sheet, ovum distributing netting is tiled in ovum distributing pool earlier, treat fertilized egg cohere on the net sheet firm after, put into the hatchery and hatch.
Larvae hatching method of adhesive egg of the present invention can also adopt following technical measures:
Described larvae hatching method of adhesive egg is characterized in: fertilized egg is distributed in long 40-120CM, on the polythene net sheet of wide 30-100CM.
Described larvae hatching method of adhesive egg is characterized in: fertilized egg is distributed in band frame polythene net sheet, and mesh is the 10-30 order.
Described larvae hatching method of adhesive egg is characterized in: the time that fertilized egg is distributed in the net sheet is no more than 2 minutes.
Described larvae hatching method of adhesive egg is characterized in: when fertilized egg was distributed in the net sheet, the clear water of ovum distributing pool was 10-20CM deeply, and the net sheet is at underwater 2-8 CM.
Described larvae hatching method of adhesive egg is characterized in: after fertilized egg was distributed in the net sheet, ovum distributing netting was tiled in ovum distributing pool interior 20-30 minute, and then, through soaking and rinsing elimination mucus, the taking-up extension the hatchery and hatched.
Advantage that the present invention has and good effect: owing to adopted technical scheme of the present invention, the viscid egg hatching has comprised the parent population cultivation, hasten parturition, insemination and hatching process, particularly evenly coat fertilized fish roe on the polythene net sheet within a certain period of time, use this kind method gained fertilized egg and do not have the attachment that influence is breathed, bond strength is higher than the fertilized egg of natural spawning, difficult drop-off, be more conducive to hatching, culture density per unit water body can reach every cubic metre 470,000 tail, average fertilization rate 94.54%, survival rate of seedling is more than 80%, by the gross time nearly 9,000 ten thousand tails of the maximum amount of growing seedlings.The present invention not only is suitable for extensive centralized production in enormous quantities, also can carry out continuous multiple batches of production; It is simple, easy to operate to have process, safely and efficiently advantage.
Description of drawings
The ovum distributing netting structural representation that Fig. 1 uses for the present invention;
Fig. 2 is the A-A cross-sectional view of Fig. 1.
Wherein, 1-polythene net, 2-metal framework.
Embodiment
For further understanding summary of the invention of the present invention, characteristics and effect, exemplify following examples now, and be described in detail as follows:
Embodiment 1
Larvae hatching method of adhesive egg comprises that parent population is cultivated, hastens parturition, insemination and hatching process:
1. the cultivation of parent population
Carry out parent fish selection before surviving the winter in the fall, proterties standard, parent population that development degree is good are put into propagating and cultivating pond early.Begin to throw something and feed when early stage water temperature was more than 9 ℃ in 1 year, main feed is maize pulp and Fructus Hordei Germinatus etc.The initial stage feeding volume accounts for 0.2% of fish body weight, along with the raising increase feeding volume of water temperature.In the time of to water temperature stability to 17 ℃, regularly bath inflation of beginning.Stop feeding in the last week of hastening parturition, bath every day, the bath time was increased to 3 hours gradually by 1 hour.Can begin to select carp crucian parent population in the time of to water temperature stability to 18 ℃ hastens parturition.
2. hasten parturition
(1) hasten parturition the determining of time
Reach the suitable water temperature of breeding in water temperature after, promptly can begin to hasten parturition, because the speeding up of the gonadal maturation by the intensification parent population, pass through stimulation by running water again, normal hatching production can be guaranteed,, just hastening parturition can be begun the carp crucian in 1-5 day in the April in every year.
(2) hasten parturition
The carp crucian promptly can be hastened parturition after reaching the suitable water temperature of breeding in water temperature.When hastening parturition, select belly and expand softly, the raun of sense of movement and the milter of ripe appropriateness are arranged, put into spawning inducing pool.The male and female of parent population are than being 4-5: 1.Establish the gentle stone supplemental oxygen of gas ducting in the spawning inducing pool, will regularly measure water temperature and dissolved oxygen during hastening parturition, but also will not have bath intermittently.The medication of hastening parturition is DOM, LRH-A
2And HCG, successively decrease gradually according to water temperature and parent population maturity adjustment dosage.Dosage is respectively DOM 2-0.4mg, and LRH-A 2-0.4ug, HCG 1000-800I.U. hasten parturition and preferably take biphasic injection, and the first injection amount is the 1/5-1/3 of total amount; The disposable injection of milter, dosage are 1/3 of raun, inject for the second time simultaneously raun and carry out, and the temperature of hastening parturition preferably remains on more than 19 ℃.
3. insemination and cloth ovum
With reference to accompanying drawing 1 and Fig. 2.After carp crucian parent population reaches the effector phase of laying eggs, (mainly show as and occur mucus in the spawning inducing pool and obviously chase phenomenon), parent population is caught out, with the cloth clamp wiped clean of dry towel fish body and dress fish.Fish-egg is clamp-oned in the dried basin then, clamp-oned seminal fluid then, the jog basin mixes smart ovum.In ovum distributing pool, fertilized egg is evenly distributed in long 80CM, wide 60CM is on the 25 purpose polythene net sheets.As attached illustrated in figures 1 and 2,1 is metal framework, and 2 is polythene net.Require per kilogram fertilized egg cloth 12-14 sheet net sheet, fertilized egg must be evenly distributed, and caking can not occur, and one deck preferably tiles on the net sheet.In the strict control time in operating process, every basin collection ovum time must not surpass 5 minutes, and weight must not surpass 1 kilogram, and the cloth ovum time must not surpass 2 minutes, so as not to smart ovum when mixing anoxic and fertilized egg bond.Requiring during the cloth ovum has the dark clear water of 10-20CM in the ovum distributing pool, and the net sheet will be convenient to make fertilized egg being evenly distributed about the 5CM of underwater.Net sheet behind the cloth ovum was tiled in the ovum distributing pool 20-30 minute, and fertilized egg is cohered on the net sheet firmly, and by soaking and mucus is eliminated in rinsing, avoided polluting the water quality of hatchery, and the taking-up extension the hatchery and hatched then.
4. hatching:
Before carp crucian collection ovum net sheet is gone into the pond,, inflate earlier with filling with clear water in the hatchery.Then cloth ovum net sheet around is suspended on the iron wire of same hatchery, the net sheet is not had in the entry, every pond net sheet fixed amount is about 120.Between the incubation period, should uninterruptedly inflate, keep dissolved oxygen about 7-8mg/l.After beginning to hatch 20-24 hour taken out the net sheet and placed in the sterilization pool, carries out the sterilization of short time high concentration with malachite green and formaldehyde etc., and disinfecting time is 5-20 minute.Simultaneously the water in the hatchery is put only, with the potassium permanganate sterilization, the net sheet after will sterilizing with current is then rinsed well, put back to the hatchery, water filling and to net sheet water-sprinkling promptly keeps the moistening of ovum further ovum to be cleaned again, to be cleaned finishing is with discharge outlet sealing, poultry full water.Hatch to 40-44 hour, above-mentioned work repeats once again.When embryonic development to muscle effector phase (53-58 hour), beginning is gradually to the continuously a small amount of water inlet in hatchery, and with the outside draining of siphon pipe.To rupture of membranes early stage, it is maximum that quantity of exchanged water reaches, and the control of every pond quantity of exchanged water is increased to 3 cubic meters/hours gradually by 0.5 cubic meters/hour, can in time discharge after making the egg membrane dissolving, with organic matter, ternary nitrogen and other harmful substance contents in the control hatchery; Alleviate violent the stirring up and down of hatching water by reducing aeration quantity simultaneously, in order to avoid can not be attached to behind the fry rupture of membranes on pool wall and the net sheet, causing drifts with the tide dies of exhaustion.In the fry rupture of membranes the time successively, the consumption of dissolved oxygen is sharply increased, must dissolved oxygen must be remained on more than the 6mg/l before and after emerging by inflation and the reasonable adjusting of changing water, preferably keep 7-8mg/l, the hatching water temperature requires to be controlled at about 22 ℃.
5. the collection of spray
Sprays such as carp crucian grow waist point appears and after, can go out the pond, Chi Shui is reduced to about 30 centimetres going out Chi Qianxian, carry out full pond with 120 order net sheets then to fish for, action wants light during operation, speed suitably slows down, in order to avoid make fry death.