CN103190367A - Babylonia lutosa egg capsule collection method and egg collector - Google Patents

Babylonia lutosa egg capsule collection method and egg collector Download PDF

Info

Publication number
CN103190367A
CN103190367A CN201310118100XA CN201310118100A CN103190367A CN 103190367 A CN103190367 A CN 103190367A CN 201310118100X A CN201310118100X A CN 201310118100XA CN 201310118100 A CN201310118100 A CN 201310118100A CN 103190367 A CN103190367 A CN 103190367A
Authority
CN
China
Prior art keywords
oopod
plastic plate
film
plastic
egg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310118100XA
Other languages
Chinese (zh)
Inventor
郑雅友
曾志南
刘波
李正良
李雷斌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian Fisheries Research Institute
Original Assignee
Fujian Fisheries Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Fisheries Research Institute filed Critical Fujian Fisheries Research Institute
Priority to CN201310118100XA priority Critical patent/CN103190367A/en
Publication of CN103190367A publication Critical patent/CN103190367A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Landscapes

  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a Babylonia lutosa egg capsule collection method and an egg collector. The Babylonia lutosa egg capsule collection method includes: step10 parent shellfishes are placed into a pool, sands are paved at the bottom of the pool, and baits are thrown into the pool to feed the parent shellfishes; step 20 the egg collector is placed in the pool, wherein the egg collector comprises a plastic board, and a film is attached to the plastic board; step 30 the parent shellfishes lay eggs, and the laid Babylonia lutosa egg capsules are attached to the film; and step 40 the egg collector is taken out, and the film attached with the egg capsules is torn off from the plastic board and placed into an incubator. The Babylonia lutosa egg capsule collection method has the advantages that the plastic board is in accordance with characteristics of the Babylonia lutosa egg capsules and is simple to produce and cheap and durable in materials; the film is adopted so that the method is simple and low in cost; and egg taking is fast and the taken eggs are integral.

Description

A kind ofly collect the method for mud east wind spiral shell egg capsule and adopt egg apparatus
Technical field
The present invention relates to a kind ofly collect the method for mud east wind spiral shell egg capsule and adopt egg apparatus.
Background technology
The collection of egg capsule is first link that mud east wind spiral shell artificial breeding begins, the parent shellfish is supported temporarily in indoor cement pit, basin bottom is covered with sand bed 8-10cm, depth of water 70cm rice, flowing water inflation, the top, pond is provided with the sunshade net of 90% shading rate, avoid the sunlight direct projection, after fortification is after a while cultivated, begin natural mating and lay eggs, lay eggs and how to carry out at night, also have minority by day.Female close shellfish can select smooth object plane to lay eggs, therefore institute's capsule of laying eggs is attached on the pool wall at the bottom of the pond about 20cm more, now general nursery adopts the plastic water pipe of diameter 11cm, long 15~20cm for adopting egg apparatus, adopt ovum above being put in pond bed-sit layer, the egg capsule that so close shellfish produces just most of producing is being adopted on the egg apparatus.The Shi Qinbei of laying eggs is attached on the pool wall or adopts egg apparatus, and mobile lentamente, ovum is slowly discharged from the abdominal foot mouth, move while laying eggs, the egg capsule of output sticks to pool wall and adopts on the egg apparatus, and mud east wind capsule that spiral shell is laid eggs belongs to the stockless egg capsule, capsule in the form of sheets, jelly strings egg capsule, and every string has 20~100 egg capsules not wait, and the upper end width is slightly littler than the base portion that adheres to, approximate trapezoid, the egg capsule upper end is convexly curved, like the shape of a saddle, and the about 6mm-9mm of the width of upper end; Adherance width 7mm-11mm; High about 4mm-8mm; Thickness 1mm, each egg capsule is about 1.2mm at interval.Institute lays eggs that capsule is arranged in a linear or arc shooting, and many egg capsules are arranged is that whole string overlaps.See through coating and can see cervine fertilized egg, the egg follicle of output is rolled in the white transparent egg capsule, soaks in the transparent colloidal fluid in egg capsule.Because the short and small stockless of mud east wind spiral shell egg capsule, collect comparatively difficulty of egg capsule, the close shellfish capsule of laying eggs continuously must be gathered once in 3-6 days, will originate in pool wall and adopt on the egg capsule on the egg apparatus to scrape with sharp cutter and get; The egg capsule of gathering is rinsed well in the incubator that is placed on the 0.3cm mesh with filtering sea, for example in the plastic basket, plastic basket is that buoyancy floats on and hatches in the pond in growing seedlings with the plastic foam, treats plastic basket (together with the egg capsule shell) to be taken out after larva hatches out again, finishes hatching process.Adopt sharp cutter from pool wall and adopt egg apparatus and scrape and get egg capsule, gather difficulty, and accidentally can cut the egg capsule bag, make the fertilized egg in the egg capsule can't continue to grow and form bad ovum.
Summary of the invention
The invention provides and a kind ofly collect the method for mud east wind spiral shell egg capsule and adopt egg apparatus, it has overcome the existing deficiency of method of collecting mud east wind spiral shell egg capsule in the background technology.
One of technical scheme that adopts that the present invention solves its technical problem is:
A kind of method of collecting mud east wind spiral shell egg capsule comprises:
Step 10 places the pond with close shellfish, and the shop is husky at the bottom of the pond in pond, the bait throwing in feeding;
Step 20 will be adopted egg apparatus and will be placed in the pond, and this is adopted egg apparatus and includes plastic plate, and film has been sticked on this plastic plate;
Step 30, close shellfish lays eggs, and the mud east wind spiral shell egg capsule of output sticks on the film;
Step 40 is taken out and is adopted egg apparatus, and tearing from plastic plate adheres to the film of egg capsule, and the film that adheres to egg capsule is put into incubator.
Among one preferred embodiment: this film is preservative film.
Among one preferred embodiment: this oopod becomes tabular.
Among one preferred embodiment: this oopod becomes " people " word shape, and this oopod comprises two plastic plates, and the top of this two plastic plate is fixed together two plastic plates to be formed " people " word shape structure; These plastic plate both sides film that all has been sticked.
Among one preferred embodiment: this oopod becomes " U " word shape, and this oopod comprises three plastic plates, and this three plastic plate is fixed together that three plastic plates are formed to fall " U " word shape structure; These plastic plate both sides film that all has been sticked.
Among one preferred embodiment: this oopod comprises a plastic plate, film has been sticked on this plastic plate front, this oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, and this plastic plate is fixed on pool wall by plastic buckle, and those oopod strings are lined up annular and adaptive pool wall.
Among one preferred embodiment: this oopod has two kinds and be divided into first oopod at least and second oopod at least;
This first oopod becomes tabular and comprises a plastic plate, film has been sticked on this plastic plate front, this first oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, and this first oopod is fixed on pool wall by plastic buckle, and those first oopod strings are lined up annular and adaptive pool wall;
This second oopod becomes " people " word shape, and this second oopod comprises two plastic plates, and the top of this two plastic plate is fixed together two plastic plates to be formed " people " word shape structure; These plastic plate both sides film that all has been sticked, this second oopod is seated on the sand.
Among one preferred embodiment: this oopod has two kinds and be divided into first oopod at least and the 3rd oopod at least;
This first oopod becomes tabular and comprises a plastic plate, film has been sticked on this plastic plate front, this first oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, and this first oopod is fixed on pool wall by plastic buckle, and those first oopod strings are lined up annular and adaptive pool wall;
The 3rd oopod becomes " U " word shape, and the 3rd oopod comprises three plastic plates, and this three plastic plate is fixed together that three plastic plates are formed to fall " U " word shape structure; These plastic plate both sides film that all has been sticked, the 3rd oopod is seated on the sand.
Among one preferred embodiment: flow and to change water and water temperature control at 22 ℃-28 ℃ in this pond.
The present invention solve its technical problem the technical scheme that adopts two be:
A kind of egg apparatus of adopting of collecting mud east wind spiral shell egg capsule includes plastic plate, and film has been sticked on this plastic plate.
The technical program is compared with background technology, and it has following advantage:
1, adopts egg apparatus and include plastic plate, film has been sticked on this plastic plate, when gathering egg capsule, tear from plastic plate and to adhere to the film of egg capsule, the film that will adhere to egg capsule is put into incubator again, can finish collection, then: select plastic plate to meet the mud east wind spiral shell capsule characteristics of laying eggs, and make simply, material is cheaply durable; Select film for use, simple cost is low; It is fast and complete to get ovum, can be from adopting the complete egg capsule that takes off of egg apparatus, can significantly shorten again the adopting the egg capsule time of close Bei Chi, and can reduce the interference to close shellfish reproductive behavior.
2, first oopod is perpendicular is inserted in husky go up and pool wall is abutted against at the back side, and those first oopod strings are lined up annular and adaptive pool wall, then can avoid egg capsule is originated in pool wall.
3, this film is preservative film, and cost is low, and is convenient to tear from plastic plate.
4, this oopod one-tenth " people " word shape or " U " word shape that falls are convenient to place, and are convenient to close shellfish and lay eggs.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 has illustrated the structural representation of first oopod.
Fig. 2 has illustrated the structural representation of second oopod.
Fig. 3 has illustrated the structural representation of second oopod.
Embodiment
Please refer to Fig. 1, Fig. 2 and Fig. 3, a kind of egg apparatus of adopting of collecting mud east wind spiral shell egg capsule includes plastic plate, and film has been sticked on this plastic plate.The egg apparatus of adopting of present embodiment is divided into three kinds, and is respectively first and adopts egg apparatus, second and adopt egg apparatus, the 3rd and adopt egg apparatus.
This first is adopted egg apparatus and becomes tabular and comprise a plastic plate 10, the front of this plastic plate 10 film 20 that has been sticked.
This second oopod becomes " people " word shape, and this second oopod comprises two plastic plates 10 ', and the top of this two plastic plate is fixed together two plastic plates to be formed " people " word shape structure; These plastic plate 10 ' both sides film 20 ' that all has been sticked.A slot is established in the top of this plastic plate, and this another piece plastic plate is convexly equipped with an insertion portion, and this insertion portion inserts in the slot affixed to realize.
The 3rd oopod becomes " U " word shape, and the 3rd oopod comprises three plastic plates, and this three plastic plate is fixed together that three plastic plates are formed to fall " U " word shape structure; These plastic plate both sides film that all has been sticked.This film is preservative film, not only not as limit, also can adopt other film as required.
A kind of method of collecting mud east wind spiral shell egg capsule comprises:
Step 10 places the pond with close shellfish, and the shop is husky at the bottom of the pond in pond, the bait throwing in feeding; Among the present embodiment, screening sea area cage is caught close shellfish: the long 39-71mm of body, and body weight 10-65g, several (close shellfish outward appearance can't be judged male and female, and some be arranged), the good shell of close shellfish vigor does not have injured.Among the present embodiment, the thick 10cm in shop husky (grain is through 0.5-1.0mm) at the bottom of the pond, the top, pond is provided with the sunshade net of 90% shading rate, avoids the sunlight direct projection, 1/m of gas head 2, and uninterrupted flowing water, quantity of exchanged water 200-300%.Among the present embodiment, the every day of bait throwing at dusk once, food species has: crab, oyster, hang razor clam and small fish, the 4-10% of daily ration, feeding quantity parent shellfish body weight takes the circumstances into consideration increase and decrease according to the situation of ingesting, drain Chi Shui next day, removes residual bait.
Step 20, adopt egg apparatus and second with first and adopt egg apparatus and be placed in the pond, among the present embodiment, this first oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, the plate upper end of this first oopod is fixed on pool wall with plastic buckle, and those first oopod strings are lined up annular and adaptive pool wall; This second oopod or and the 3rd oopod evenly be seated on the sand.
Step 30, close shellfish begins to lay eggs successively after cultivating a week, and the mud east wind spiral shell egg capsule of output sticks on the film; Among the present embodiment, there is the capsule of laying eggs on this lambdoid inside and outside two sides of egg apparatus of adopting, and the square on the diapire of setting pond is adopted egg apparatus also to be had, and determines every 3-6 days receipts egg capsules once according to the height of coolant-temperature gage;
Step 40, draining is taken out and is adopted egg apparatus, and tearing from plastic plate adheres to the film of egg capsule, and the film that adheres to egg capsule is put into incubator, in the hatching basket, has the plastic plate of egg capsule to stick preservative film at the product of tearing again, and replaces.
Flow and to change water and water temperature control at 22 ℃-28 ℃ in this pond.
The above, only for preferred embodiment of the present invention, so can not limit scope of the invention process according to this, i.e. the equivalence of doing according to claim of the present invention and description changes and modifies, and all should still belong in the scope that the present invention contains.

Claims (10)

1. method of collecting mud east wind spiral shell egg capsule is characterized in that: comprising:
Step 10 places the pond with close shellfish, and the shop is husky at the bottom of the pond in pond, the bait throwing in feeding;
Step 20 will be adopted egg apparatus and will be placed in the pond, and this is adopted egg apparatus and includes plastic plate, and film has been sticked on this plastic plate;
Step 30, close shellfish lays eggs, and the mud east wind spiral shell egg capsule of output sticks on the film;
Step 40 is taken out and is adopted egg apparatus, and tearing from plastic plate adheres to the film of egg capsule, and the film that adheres to egg capsule is put into incubator.
2. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1, it is characterized in that: this film is preservative film.
3. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1, it is characterized in that: this oopod becomes tabular.
4. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1, it is characterized in that: this oopod becomes " people " word shape, this oopod comprises two plastic plates, and the top of this two plastic plate is fixed together two plastic plates to be formed " people " word shape structure; These plastic plate both sides film that all has been sticked.
5. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1 is characterized in that: this oopod becomes " U " word shape, and this oopod comprises three plastic plates, and this three plastic plate is fixed together that three plastic plates are formed to fall " U " word shape structure; These plastic plate both sides film that all has been sticked.
6. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 3, it is characterized in that: this oopod comprises a plastic plate, film has been sticked on this plastic plate front, this oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, this plastic plate is fixed on pool wall by plastic buckle, and those oopod strings are lined up annular and adaptive pool wall.
7. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1 is characterized in that:
This oopod has two kinds and be divided into first oopod at least and second oopod at least;
This first oopod becomes tabular and comprises a plastic plate, film has been sticked on this plastic plate front, this first oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, and this first oopod is fixed on pool wall by plastic buckle, and those first oopod strings are lined up annular and adaptive pool wall;
This second oopod becomes " people " word shape, and this second oopod comprises two plastic plates, and the top of this two plastic plate is fixed together two plastic plates to be formed " people " word shape structure; These plastic plate both sides film that all has been sticked, this second oopod is seated on the sand.
8. a kind of method of collecting mud east wind spiral shell egg capsule according to claim 1 is characterized in that:
This oopod has two kinds and be divided into first oopod at least and the 3rd oopod at least;
This first oopod becomes tabular and comprises a plastic plate, film has been sticked on this plastic plate front, this first oopod is perpendicular to be inserted in husky go up and pool wall is abutted against at the back side, and this first oopod is fixed on pool wall by plastic buckle, and those first oopod strings are lined up annular and adaptive pool wall;
The 3rd oopod becomes " U " word shape, and the 3rd oopod comprises three plastic plates, and this three plastic plate is fixed together that three plastic plates are formed to fall " U " word shape structure; These plastic plate both sides film that all has been sticked, the 3rd oopod is seated on the sand.
9. according to each described a kind of method of collecting mud east wind spiral shell egg capsule in the claim 1 to 8, it is characterized in that: flows and to change water and water temperature control at 22 ℃-28 ℃ in this pond.
10. egg apparatus of adopting of collecting mud east wind spiral shell egg capsule, it is characterized in that: include plastic plate, film has been sticked on this plastic plate.
CN201310118100XA 2013-04-07 2013-04-07 Babylonia lutosa egg capsule collection method and egg collector Pending CN103190367A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310118100XA CN103190367A (en) 2013-04-07 2013-04-07 Babylonia lutosa egg capsule collection method and egg collector

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310118100XA CN103190367A (en) 2013-04-07 2013-04-07 Babylonia lutosa egg capsule collection method and egg collector

Publications (1)

Publication Number Publication Date
CN103190367A true CN103190367A (en) 2013-07-10

Family

ID=48713336

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310118100XA Pending CN103190367A (en) 2013-04-07 2013-04-07 Babylonia lutosa egg capsule collection method and egg collector

Country Status (1)

Country Link
CN (1) CN103190367A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104798709A (en) * 2015-04-22 2015-07-29 海南定利养殖种苗有限公司 Breeding method of babylonia areolata cross-breeding seeds
CN105613360A (en) * 2014-11-04 2016-06-01 中国科学院海洋研究所 Egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and use method of devices
CN117652438A (en) * 2023-11-03 2024-03-08 海南康冠生物科技有限公司 Method for promoting copulation and spawning of Babylonia and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58134926A (en) * 1982-02-05 1983-08-11 電源開発株式会社 Artificial fish bank
CN1593112A (en) * 2004-07-12 2005-03-16 天津农学院 Glutinous egg and fry hatching method
JP2006304677A (en) * 2005-04-28 2006-11-09 Hiroshima Pref Gov Tool for collecting seedling of larva of sessile shellfishes
CN102257977A (en) * 2011-07-20 2011-11-30 浙江省海洋水产研究所 Method for acquiring and hatching fertilized ova of fish
CN202168404U (en) * 2011-07-20 2012-03-21 浙江省海洋水产研究所 Attaching device for fish fertilized eggs
CN202476273U (en) * 2012-03-21 2012-10-10 东北农业大学 Fish nest for spawning and incubation of fishes laying adhesive eggs

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58134926A (en) * 1982-02-05 1983-08-11 電源開発株式会社 Artificial fish bank
CN1593112A (en) * 2004-07-12 2005-03-16 天津农学院 Glutinous egg and fry hatching method
JP2006304677A (en) * 2005-04-28 2006-11-09 Hiroshima Pref Gov Tool for collecting seedling of larva of sessile shellfishes
CN102257977A (en) * 2011-07-20 2011-11-30 浙江省海洋水产研究所 Method for acquiring and hatching fertilized ova of fish
CN202168404U (en) * 2011-07-20 2012-03-21 浙江省海洋水产研究所 Attaching device for fish fertilized eggs
CN202476273U (en) * 2012-03-21 2012-10-10 东北农业大学 Fish nest for spawning and incubation of fishes laying adhesive eggs

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
吴进锋等: "台湾东风螺人工繁殖及苗种生物学的初步研究", 《海洋科学》 *
王如才等: "《海水贝类养殖学》", 31 July 2008, 中国海洋大学出版社 *
王清印: "《海水健康养殖与水产品质量安全》", 30 September 2006, 海洋出版社 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105613360A (en) * 2014-11-04 2016-06-01 中国科学院海洋研究所 Egg bag collecting and hatching devices for industrial seedling culture of Rapana venosa and use method of devices
CN104798709A (en) * 2015-04-22 2015-07-29 海南定利养殖种苗有限公司 Breeding method of babylonia areolata cross-breeding seeds
CN104798709B (en) * 2015-04-22 2017-05-03 海南定利养殖种苗有限公司 Breeding method of babylonia areolata cross-breeding seeds
CN117652438A (en) * 2023-11-03 2024-03-08 海南康冠生物科技有限公司 Method for promoting copulation and spawning of Babylonia and application thereof

Similar Documents

Publication Publication Date Title
CN202112155U (en) Abalone and sea cucumber stereoscopic ecological culturing net cage
CN105123565B (en) A kind of cultchless oyster Deepwater Open Sea cultural method and device
KR102355838B1 (en) Method of culturing organism to be cultured, and culturing facility
CN103026988B (en) Indoor intensive artificial breeding technique of Yangtze River Coilia ectenes
CN103636540B (en) A kind of ecological early breeding method of Coilia ectenes in Yangtze River pond greenhouse
CN108901965A (en) A kind of large size cage-farming classification fishes device and its large-scale net cage and method
CN102550457A (en) Early propagation method for hyriopsis cumingii seeds
CN104430113B (en) A kind of method of bighead cultivation domestication feed table cultivation domestication bighead
CN103202248A (en) Mixed culture method for fishes, prawns and crabs in reed zone ditch
CN107155999A (en) A kind of dim light induces green fin black scraper Puffer juvenile fish cluster to feed and pool device and method
CN106942103B (en) Artificial seedling raising method for cucurbit snails
CN104255613A (en) Method of freshwater aquaculture of Litopenaeus vannamei in net cage
CN103190367A (en) Babylonia lutosa egg capsule collection method and egg collector
CN102017920A (en) Method for collecting indoors cultivated natural patinopecten yesoensis larvae by trawling
CN102077788B (en) Method for improving culture survival rate of river crabs by using climbing device
CN101703034B (en) Three-dimensional seedling cultivating method of octopus variabilis
CN104663541B (en) Artificial breeding method for cultellus attenuatus
JP4915946B2 (en) Seaweed growth method
CN103535296B (en) Method for cultivating young rapana venosa in rapana venosa artificial breeding
CN104304101B (en) The factorial seedling-culturing method of Hemi fusus tuba (Gmelin)
CN202760008U (en) Soil pond rearing device of Chinese mitten crab large-scale family juvenile crabs
CN101990853B (en) Artificial breeding method of Spisula sachalinensis (Schrenck)
CN103609489A (en) Method for snakehead cage culture
CN210671708U (en) Bionical attitude loach breeds device
CN207284768U (en) A kind of hatching cage for being suitable for placing Odontobutis obscura tube for laying eggs

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20130710