CN104663541B - Artificial breeding method for cultellus attenuatus - Google Patents
Artificial breeding method for cultellus attenuatus Download PDFInfo
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- CN104663541B CN104663541B CN201510052041.XA CN201510052041A CN104663541B CN 104663541 B CN104663541 B CN 104663541B CN 201510052041 A CN201510052041 A CN 201510052041A CN 104663541 B CN104663541 B CN 104663541B
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- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 241000439122 Cultellus attenuatus Species 0.000 title abstract 8
- 238000000034 method Methods 0.000 claims abstract description 23
- 241000237852 Mollusca Species 0.000 claims abstract description 15
- 230000000366 juvenile effect Effects 0.000 claims abstract description 15
- 238000011534 incubation Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 73
- 235000015170 shellfish Nutrition 0.000 claims description 46
- 241000091751 Chaetoceros muellerii Species 0.000 claims description 15
- 241000206751 Chrysophyceae Species 0.000 claims description 15
- 230000001488 breeding effect Effects 0.000 claims description 15
- 241000195493 Cryptophyta Species 0.000 claims description 13
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 12
- 235000013601 eggs Nutrition 0.000 claims description 12
- 230000001418 larval effect Effects 0.000 claims description 10
- 239000013535 sea water Substances 0.000 claims description 10
- 230000018109 developmental process Effects 0.000 claims description 6
- 210000002149 gonad Anatomy 0.000 claims description 6
- 208000026487 Triploidy Diseases 0.000 claims description 5
- 230000009193 crawling Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 210000000936 intestine Anatomy 0.000 claims description 5
- 238000005259 measurement Methods 0.000 claims description 5
- 230000029052 metamorphosis Effects 0.000 claims description 5
- 238000000386 microscopy Methods 0.000 claims description 5
- 244000045947 parasite Species 0.000 claims description 5
- 210000002784 stomach Anatomy 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000004083 survival effect Effects 0.000 abstract description 2
- 241000237502 Ostreidae Species 0.000 abstract 2
- 235000020636 oyster Nutrition 0.000 abstract 2
- 230000006698 induction Effects 0.000 abstract 1
- 210000004681 ovum Anatomy 0.000 description 7
- 210000004907 gland Anatomy 0.000 description 6
- 230000001568 sexual effect Effects 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000269800 Percidae Species 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to an artificial breeding method for cultellus attenuatus. The artificial breeding method for cultellus attenuatus is characterized by mainly comprising the following steps of (1) cultivation of parent oysters, (2) artificial spawning, (3) artificial incubation, (4) cultivation of 'D'-shaped larvas, (5) cultivation of juvenile mollusks and (6) emergence of seedlings. The cultellus attenuatus of which the average length of shells is 1.40-1.50cm can be obtained after the cultellus attenuatus is cultivated for 30-40 days. Compared with the prior art, the artificial breeding method for the cultellus attenuatus has the advantages that the breeding method for the cultellus attenuatus is researched successfully, the technological process is scientific and simple, the operability is high, by the steps of cultivation of the parent oysters, artificial spawning, artificial incubation, cultivation of the 'D'-shaped larvas and cultivation of the juvenile mollusks, the spawning induction emission ratio reaches 50%, the hatchability is 80%, the total survival rate reaches 8-12%, more than 8,350,000 seedlings of which the shell lengths are 1.59mm can be bred, the artificial breeding method for the cultellus attenuatus is creative, and the artificial breeding method provides a basis for large-scale seedling production.
Description
Technical field
The present invention relates to a kind of artificial breeding technique of aquaculture, especially a kind of artificial raise seedling method of pocket knife razor clam.
Background technology
Pocket knife razor clam is very wide in China's distribution, and North gets Liaodong Peninsula, south is all distributed to Hainan, often perches in intertidal zone from tide
98 meters of lower band, is one of marine shellfish liked of behaving, occupies considerable status in edible shellfish.Recently as people
Growth in the living standard, the increase to the seafood demand such as bivalve shellfish leads to excessive amount of fishing, along with offshore pollution is increasingly tight
Weight, leads to pocket knife razor clam resource gradually to fail, quantity of the catch increasingly reduces, and moves outside fishing ground, and individuality becomes little, and Natural Resources are not allowed to find pleasure in
See, therefore, the research carrying out the artificial breeding technique of pocket knife razor clam is extremely urgent.But current, do not have both at home and abroad to pocket knife razor clam people
Work method for culturing seedlings is studied, the lack of checks on power development of China's pocket knife razor clam aquaculture of seed source.
Content of the invention
The technical problem to be solved is a kind of pocket knife razor clam of the present situation high yield of offer for prior art
Artificial raise seedling method, does not have artificial raise seedling method to solve pocket knife razor clam, can only rely on the problem of natural seizure.
The present invention solves the technical scheme that adopted of above-mentioned technical problem：The artificial raise seedling method of this pocket knife razor clam, it is special
Levy and be：The artificial raise seedling method of described pocket knife razor clam mainly comprises the steps：
(1) cultivate close shellfish：Select 7～September full mature gonad, shell a length of (7.44 ± 0.52) cm, shell a width of (2.39 ±
0.17) cm, the pocket knife razor clam having no parasite carries out artificial breeding as close shellfish；
(2) artificial induced spawning：The close shellfish that will select in step (1), clean through filtering seawater flushing, after the 10～12h that dries in the shade,
Close shellfish is put in net cage and is laid eggs, in each net cage, put 400～500 about close shellfishes, that is, control close shellfish in net cage
Density is 1600～2000/m3, net cage is suspended in spawning pond；
(3) artificial incubation：By the close shellfish in step (2) in 6～7h about start lay eggs, control triploidy rate be 30～
40/ml, hatched, control the water temperature of hatching to be 25～27 DEG C, salinity is 20～23 ‰；
(4) " D " type larval culture：After 22～24 hours development of fertilized ova be in line the stranded young be " D " type young, simultaneously
Control young density is 10～15/ml, and during feeding equilateral chrysophyceae (4000～5000cells/ml), larval culture, water
Temperature is 23～25 DEG C, salinity 24～26 ‰, daily microscopy 1～3 time, checks stomach and intestine plumpness, and estimates young quantity, measurement children
The size of body, in " D " shape young to shell top deutovum, changes water 2～3 times daily, changes water 50～60% daily, the depth of water controls
1～3m, and feed (4000～5000cells/ml)：(2000～3000cells/ml)：(2000～3000cells/ml's)
Equilateral chrysophyceae, chlorella and Chaetoceros muelleri, to the shell top larva later stage, water is changed in full pond, and the depth of water controls in 1～2m, attachment
Afterwards, change water daily twice, quantity of exchanged water is 50～60%, and the depth of water controls in 0.5～1m, thoroughly change 1 attachment within 5～6 days
Base, and feed (10000～15000cells/ml)：(8000～10000cells/ml)：(8000～10000cells/ml)：
The equilateral chrysophyceae of (500～1000cells/ml), chlorella, Chaetoceros muelleri and flat algae；
(5) juvenile mollusk is cultivated：When paedomorphosis to shell top larva later stage, during a length of 0.198 ± 0.011mm of shell, eyespot occurs,
Beginning preparing for attachment base, after having more than 70% larva that eyespot occurs, throw attachment base in time, the thickness of described attachment base is 1～
5cm, after water is limpid, moves into larva and carries out settlement and metamorphosis, and throw in foundation bait (10000～15000cells/ml)：
(10000～15000cells/ml)：The chlorella of (1000～1500cells/ml), Chaetoceros muelleri and flat algae, control juvenile mollusk
Cultivate water temperature and be 22～25 DEG C, salinity 24～26 ‰；
(6) emerge：Through the cultivation of 30～40 days, that is, the pocket knife razor clam obtaining a length of 1.40～1.50cm of average shell was just permissible
Emerge, carry out middle culture to outdoor Tu Tang.
Need when emerging to be consistent the water temperature of indoor water temperature and outdoor breeding, prevent temperature shock, cause
The large quantities of death of seed.
Further, the release time of the attachment base in described step (4) occurs to crawling for shell top larva later stage eyespot
Between the young, needs 70% appear above to proceed by during eyespot and feed attachment base, and the described shell top larva later stage develops and crawls
During the young, a length of 0.245 ± 0.016mm of shell.
Further, attachment base described in described step (5) is the ooze of intertidal zone, and ooze, through drying, grinds, plus
Enter sea water filter, after heating is boiled, be cooled to normal temperature, when described attachment base is thrown in, must uniformly rinse in pond,
It is made all to precipitate, thickness 2～3cm, after water is limpid, place into the young.
As preferred：The cultivation density that in described step (4), shell top deutovum is cultivated is 8～15/ml.
As preferred：The cultivation density that in described step (5), the shell top larva later stage cultivates is 6～8/ml.
As preferred：The cultivation density that in described step (5), juvenile mollusk cultivates density is 30/ml.
Compared with prior art, it is an advantage of the current invention that：The present invention successfully investigated a kind of nursery side of pocket knife razor clam
Method, technological process science is simple, workable, present invention employs close shellfish and cultivates, artificial induced spawning, artificial incubation, " D " type children
Body is cultivated, the step that juvenile mollusk is cultivated, and emission index of hastening parturition reaches 50%, incubation rate 80%, and total survival rate reaches 8～12%, educates flat altogether
All the young shellfish of the long 1.59mm of shell amounts to more than 8,350,000 grains it is achieved that breakthrough in artificial breeding pocket knife razor clam method, for realizing on a large scale
Seedling production is offered reference foundation.
Brief description
Fig. 1 is the Annual fluctuation of pocket knife razor clam coefficient of condition；
Fig. 2 is the Annual fluctuation of pocket knife razor clam gonad development..
Specific embodiment
Below by way of with reference to drawings and Examples, the invention will be further described.
Embodiment 1
First anniversary sampling is carried out to pocket knife razor clam, take the close pocket knife razor clam of 30 size specifications, the mud that clean shell retains
Sand, will software part and shell separately, be put in 48 hours in 60 DEG C of baking oven, with electronic balance weighing software dry weight and shell dry weight,
Coefficient of condition=(software dry weight/shell dry weight) × 100%, to determine the concrete spawning season of pocket knife razor clam, result as illustrated in fig. 1 and 2, from
As can be seen that in April November to next year, coefficient of condition change is inconspicuous, to May, June, coefficient of condition rapid increase in Fig. 1；July
In the first tenday period of a month, some individuals start to lay eggs, and late July, most of individuality has been laid eggs, and coefficient of condition drastically declines, the grossness with April
Degree is close；Early August, coefficient of condition starts to raise again, and sexual gland returns to the maturity period quickly, and enters the next egg-laying season, 7
Month to during September, all can carry out artificial induced spawning, carry out artificial breeding；
From figure 2 it can be seen that pocket knife razor clam sexual gland at the beginning of last month of spring in winter started to change to proliferation period from stand-down, cell starts to increase
Long.And sexual gland is almost ripe after the summer has set in that arrives, wherein June when maturity of fish gonads reach highest.To between 7 to September, little
The sexual gland of knife razor clam is continuously in the draining period stage it is seen that 7～September is the breeding season of pocket knife razor clam, and artificial breeding research should be focused on
Note this period.Late September, sexual gland again went into stand-down, to treat the cyclostage next time until October.Fig. 2 shows
Show, the gonad development of pocket knife razor clam has obvious seasonal variety, and its sexual gland reaches full maturity in June, and its reproduction season
Save as 7～September part.
Specifically the artificial raise seedling method of this pocket knife razor clam mainly comprises the steps：
(1) cultivate close shellfish：Late July to early September is selected to buy full mature gonad, a length of 7.96cm of shell, shell width
For 3.07cm, the pocket knife razor clam having no parasite carries out artificial breeding parent shellfish it is ensured that it had not produced ovum as close shellfish, to reduce into
This；
(2) artificial induced spawning：The close shellfish that will select in step (1) is clean through filtering seawater flushing, after the 12h that dries in the shade, parent
Shellfish is put in net cage (150cm × 80cm × 20cm) and is laid eggs, and puts 400 about close shellfishes, that is, control close shellfish in each net cage
Density in net cage is 1700/m3；
(3) artificial incubation：By the close shellfish in step (2) in 6h about start lay eggs, control triploidy rate be 30/
Ml, is hatched, and controls the water temperature of hatching to be 25 DEG C, salinity is 23 ‰；
(4) " D " type larval culture：After 24 hours development of fertilized ova be in line the stranded young be " D " type young, control simultaneously
Young density is 10/ml, and feeds the equilateral chrysophyceae of 4000cells/ml, and during larval culture, water temperature is 23 DEG C, salinity
24 ‰, daily microscopy 1 time, check stomach and intestine plumpness, and estimate young quantity, the size of the measurement young, in " D " shape young to shell
Top deutovum, controls the cultivation density to shell top deutovum to be 10/ml simultaneously, changes water 2 times daily, change water daily
50%, the depth of water controls in 2m, and feeds 4000cells/ml：2000cells/ml：The equilateral chrysophyceae of 2000cells/ml, bead
Algae and Chaetoceros muelleri, to the shell top larva later stage, water is changed in full pond, and the depth of water controls in 2m, after attachment, changes water daily twice,
Quantity of exchanged water is 50%, and the depth of water controls in 1m, thoroughly changes 1 attachment base, and feeds 10000cells/ml within 5 days：
8000cells/ml：10000cells/ml：The equilateral chrysophyceae of 500cells/ml, chlorella, Chaetoceros muelleri and flat algae；
(5) juvenile mollusk is cultivated：When paedomorphosis to shell top larva later stage, control the cultivation of shell top larva later stage cultivation close simultaneously
Spend for 6/ml, during a length of 0.209mm of shell, eyespot occurs, and begins preparing for attachment base, eyespot when there being more than 70% larva
Afterwards, throw attachment base in time, the thickness of attachment base is 5cm, after water is limpid, moves into larva and carry out settlement and metamorphosis, and throw in basis
Bait 10000cells/ml：10000cells/ml：The chlorella of 1000cells/ml, Chaetoceros muelleri and flat algae, control young
Shellfish is cultivated water temperature and is 25 DEG C, salinity 24 ‰, and attachment base is the ooze of intertidal zone, and ooze, through drying, grinds, and adds seawater mistake
Filter, after heating is boiled, is cooled to normal temperature, when attachment base is thrown in, must uniformly rinse in pond so as to all sink
Form sediment, thickness 2cm, after water is limpid, place into the young, and the release time of attachment base occurs to crawling for shell top larva later stage eyespot
Between the crawl young, needs 70% appear above to proceed by during eyespot and feed attachment base, and the shell top larva later stage develops the children that crawls
During body, a length of 0.261mm of shell, control the cultivation density that juvenile mollusk cultivates density to be 30/ml simultaneously；
(6) emerge：Through the cultivation of 30 days, that is, the pocket knife razor clam obtaining a length of 1.40cm of average shell just can be emerged, to family
Outer Tu Tang carries out middle culture.Need when emerging to be consistent the water temperature of indoor water temperature and outdoor breeding, prevent temperature
Degree cataclysm, causes the large quantities of death of seed.
Embodiment 2
The artificial raise seedling method of this pocket knife razor clam mainly comprises the steps：(1) cultivate close shellfish：Select late July in September
Buy full mature gonad ten days, a length of 6.92cm of shell, a width of 2.22cm of shell, the pocket knife razor clam having no parasite is carried out as close shellfish
Artificial breeding parent shellfish was not it is ensured that it had produced ovum, to reduce cost；
(2) artificial induced spawning：The close shellfish that will select in step (1) is clean through filtering seawater flushing, after the 12h that dries in the shade, parent
Shellfish is put in net cage (150cm × 80cm × 20cm) and is laid eggs, and puts 500 about close shellfishes, that is, control close shellfish in each net cage
Density in net cage is 2000/m3；
(3) artificial incubation：By the close shellfish in step (2) in 7h about start lay eggs, control triploidy rate be 40/
Ml, is hatched, and controls the water temperature of hatching to be 27 DEG C, salinity is 20 ‰；
(4) " D " type larval culture：After 22 hours development of fertilized ova be in line the stranded young be " D " type young, control simultaneously
Young density is 15/ml, and feeds the equilateral chrysophyceae of 5000cells/ml, and during larval culture, water temperature is 25 DEG C, salinity
26 ‰, daily microscopy 2 times, check stomach and intestine plumpness, and estimate young quantity, the size of the measurement young, in " D " shape young to shell
Top deutovum, controls the cultivation density to shell top deutovum to be 15/ml simultaneously, changes water 3 times daily, change water daily
60%, the depth of water controls in 3m, and feeds 5000cells/ml：3000cells/ml：The equilateral chrysophyceae of 3000cells/ml, bead
Algae and Chaetoceros muelleri, to the shell top larva later stage, water is changed in full pond, and the depth of water controls in 2m, after attachment, changes water daily twice,
Quantity of exchanged water is 60%, and the depth of water controls in 0.5m, thoroughly changes 1 attachment base, and feeds 15000cells/ml within 6 days：
10000cells/ml：8000cells/ml：The equilateral chrysophyceae of 1000cells/ml, chlorella, Chaetoceros muelleri and flat algae；
(5) juvenile mollusk is cultivated：When paedomorphosis to shell top larva later stage, control the cultivation of shell top larva later stage cultivation close simultaneously
Spend for 8/ml, during a length of 0.187mm of shell, eyespot occurs, and begins preparing for attachment base, eyespot when there being more than 70% larva
Afterwards, throw attachment base in time, the thickness of attachment base is 1cm, after water is limpid, moves into larva and carry out settlement and metamorphosis, and throw in basis
Bait 15000cells/ml：15000cells/ml：The chlorella of 1500cells/ml, Chaetoceros muelleri and flat algae, control young
Shellfish is cultivated water temperature and is 22 DEG C, salinity 26 ‰, and attachment base is the ooze of intertidal zone, and ooze, through drying, grinds, and adds seawater mistake
Filter, after heating is boiled, is cooled to normal temperature, when attachment base is thrown in, must uniformly rinse in pond so as to all sink
Form sediment, thickness 2～3cm, after water is limpid, place into the young, and the release time of attachment base is that shell top larva later stage eyespot occurs
To crawling between the young, 70% is needed to appear above to proceed by during eyespot and feed attachment base, the shell top larva later stage develops and crawls
During the crawl young, a length of 0.229mm of shell, control the cultivation density that juvenile mollusk cultivates density to be 30/ml simultaneously；
(6) emerge：Through the cultivation of 30 days, that is, the pocket knife razor clam obtaining a length of 1.50cm of average shell just can be emerged, to family
Outer Tu Tang carries out middle culture.Need when emerging to be consistent the water temperature of indoor water temperature and outdoor breeding, prevent temperature
Degree cataclysm, causes the large quantities of death of seed.
Embodiment 3
The artificial raise seedling method of this pocket knife razor clam mainly comprises the steps：(1) cultivate close shellfish：Select late July in September
Buy full mature gonad ten days, a length of 7.00cm of shell, a width of 2.20cm of shell, the pocket knife razor clam having no parasite is carried out as close shellfish
Artificial breeding parent shellfish was not it is ensured that it had produced ovum, to reduce cost；
(2) artificial induced spawning：The close shellfish that will select in step (1) is clean through filtering seawater flushing, after the 11h that dries in the shade, parent
Shellfish is put in net cage (150cm × 80cm × 20cm) and is laid eggs, and puts 450 about close shellfishes, that is, control close shellfish in each net cage
Density in net cage is 1800/m3；
(3) artificial incubation：By the close shellfish in step (2) in 6.5h about start lay eggs, control triploidy rate be 35/
Ml, is hatched, and controls the water temperature of hatching to be 26 DEG C, salinity is 22 ‰；
(4) " D " type larval culture：After 22 hours development of fertilized ova be in line the stranded young be " D " type young, control simultaneously
Young density is 13/ml, and feeds the equilateral chrysophyceae of 4500cells/ml, and during larval culture, water temperature is 24 DEG C, salinity
25 ‰, daily microscopy 1 time, check stomach and intestine plumpness, and estimate young quantity, the size of the measurement young, in " D " shape young to shell
Top deutovum, controls the cultivation density to shell top deutovum to be 15/ml simultaneously, changes water 2 times daily, change water daily
60%, the depth of water controls in 2m, and feeds 4500cells/ml：2500cells/ml：The equilateral chrysophyceae of 2500cells/ml, bead
Algae and Chaetoceros muelleri, to the shell top larva later stage, water is changed in full pond, and the depth of water controls in 2m, after attachment, changes water daily twice,
Quantity of exchanged water is 55%, and the depth of water controls in 0.6m, thoroughly changes 1 attachment base, and feeds 12000cells/ml within 6 days：
9000cells/ml：9000cells/ml：The equilateral chrysophyceae of 500cells/ml, chlorella, Chaetoceros muelleri and flat algae；
(5) juvenile mollusk is cultivated：When paedomorphosis to shell top larva later stage, control the cultivation of shell top larva later stage cultivation close simultaneously
Spend for 8/ml, during a length of 0.188mm of shell, eyespot occurs, and begins preparing for attachment base, eyespot when there being more than 70% larva
Afterwards, throw attachment base in time, the thickness of attachment base is 3cm, after water is limpid, moves into larva and carry out settlement and metamorphosis, and throw in basis
Bait 12000cells/ml：12000cells/ml：The chlorella of 1200cells/ml, Chaetoceros muelleri and flat algae, control young
Shellfish is cultivated water temperature and is 24 DEG C, salinity 25 ‰, and attachment base is the ooze of intertidal zone, and ooze, through drying, grinds, and adds seawater mistake
Filter, after heating is boiled, is cooled to normal temperature, when attachment base is thrown in, must uniformly rinse in pond so as to all sink
Form sediment, thickness 2.5cm, after water is limpid, place into the young, and the release time of attachment base occurs arriving for shell top larva later stage eyespot
Crawl between the young, needs 70% appear above to proceed by during eyespot and feed attachment base, and the shell top larva later stage develops and crawls
During the young, a length of 0.229mm of shell, control the cultivation density that juvenile mollusk cultivates density to be 30/ml simultaneously；
(6) emerge：Through the cultivation of 30 days, that is, the pocket knife razor clam obtaining a length of 1.45cm of average shell just can be emerged, to family
Outer Tu Tang carries out middle culture.Need when emerging to be consistent the water temperature of indoor water temperature and outdoor breeding, prevent temperature
Degree cataclysm, causes the large quantities of death of seed.
Claims (6)
1. a kind of artificial raise seedling method of pocket knife razor clam it is characterised in that：The artificial raise seedling method of described pocket knife razor clam mainly includes
Following step：
(1) cultivate close shellfish：Select 7～September full mature gonad, shell a length of (7.44 ± 0.52) cm, shell a width of (2.39 ±
0.17) cm, the pocket knife razor clam having no parasite carries out artificial breeding as close shellfish；
(2) artificial induced spawning：The close shellfish that will select in step (1) is clean through filtering seawater flushing, after the 10～12h that dries in the shade, parent
Shellfish is put in net cage and is laid eggs, and puts 400～500 close shellfishes in each net cage, and that is, the close density in net cage for the shellfish of control is
1600～2000/m3, net cage is suspended in spawning pond；
(3) artificial incubation：Close shellfish in step (2) is started to lay eggs in 6～7h, control triploidy rate is 30～40/ml,
Hatched, control the water temperature of hatching to be 25～27 DEG C, salinity is 20～23 ‰；
(4) " D " type larval culture：After 22～24 hours development of fertilized ova be in line the stranded young be " D " type young, control simultaneously
Young density is 10～15/ml, and feeds the equilateral chrysophyceae of 4000～5000cells/ml, and during larval culture, water temperature is
23～25 DEG C, salinity 24～26 ‰, daily microscopy 1～3 time, check stomach and intestine plumpness, and estimate young quantity, the measurement young
Size, in " D " shape young to shell top deutovum, changes water 2～3 times daily, changes water 50～60% daily, and the depth of water controls 1～
3m, and feed (4000～5000cells/ml)：(2000～3000cells/ml)：(2000～3000cells/ml's) is equilateral
Chrysophyceae, chlorella and Chaetoceros muelleri, to the shell top larva later stage, water is changed in full pond, and the depth of water controls after 1～2m, attachment, changes daily
Twice, quantity of exchanged water is 50～60% to water, and the depth of water controls in 0.5～1m, thoroughly changes 1 attachment base, and feeds within 5～6 days
(10000～15000cells/ml)：(8000～10000cells/ml)：(8000～10000cells/ml)：(500～
Equilateral chrysophyceae 1000cells/ml), chlorella, Chaetoceros muelleri and flat algae；
(5) juvenile mollusk is cultivated：When paedomorphosis to shell top larva later stage, during a length of 0.198 ± 0.011mm of shell, eyespot occurs, and starts
Prepare attachment base, after having more than 70% larva that eyespot occurs, throw attachment base in time, the thickness of described attachment base is 1～5cm,
After water is limpid, moves into larva and carry out settlement and metamorphosis, and throw in foundation bait (10000～15000cells/ml)：(10000～
15000cells/ml)：The chlorella of (1000～1500cells/ml), Chaetoceros muelleri and flat algae, control juvenile mollusk to cultivate water temperature
For 22～25 DEG C, salinity 24～26 ‰；
(6) emerge：Through the cultivation of 30～40 days, that is, the pocket knife razor clam obtaining a length of 1.40～1.50cm of average shell just can go out
Seedling, carries out middle culture to outdoor Tu Tang.
2. pocket knife razor clam according to claim 1 artificial raise seedling method it is characterised in that：Attachment in described step (5)
The release time of base occurs to crawling between the young for shell top larva later stage eyespot, need 70% appear above start during eyespot into
Row feeds attachment base, and the described shell top larva later stage develops when crawling the young, a length of 0.245 ± 0.016mm of shell.
3. pocket knife razor clam according to claim 1 artificial raise seedling method it is characterised in that：Attached described in described step (5)
The ooze that base is intertidal zone, ooze, through drying, grinds, add sea water filter, after heating is boiled, be cooled to normal temperature
, when described attachment base is thrown in, must uniformly rinse so as to whole precipitate in pond, thickness 2～3cm, after water is limpid,
Place into the young.
4. pocket knife razor clam according to claim 1 artificial raise seedling method it is characterised in that：Shell top children in described step (4)
The cultivation density that worm early stage is cultivated is 8～15/ml.
5. pocket knife razor clam according to claim 1 artificial raise seedling method it is characterised in that：Shell top children in described step (5)
The cultivation density that the worm later stage cultivates is 6～8/ml.
6. pocket knife razor clam according to claim 1 artificial raise seedling method it is characterised in that：Juvenile mollusk training in described step (5)
The cultivation density educating density is 30/ml.
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| CN107182869A (en) * | 2017-06-05 | 2017-09-22 | 丹东市水产技术推广总站 | A kind of razor shell offspring seed cultivation method |
| CN108450379A (en) * | 2018-02-09 | 2018-08-28 | 中国海洋大学 | A kind of method that pocket knife razor clam incubation is cultivated and laid eggs |
| CN115281129B (en) * | 2022-09-15 | 2023-06-23 | 江苏省海洋水产研究所 | Method for breeding Solen grandis in stepwise cement pond |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1336108A (en) * | 2000-07-31 | 2002-02-20 | 山东省日照市水产研究所 | Artificial method for breeding giant razor cham |
| CN101731158A (en) * | 2008-11-24 | 2010-06-16 | 张明钰 | Shallow pond culture technique for sinonovacula constricta lamarck |
| CN101946737A (en) * | 2010-09-10 | 2011-01-19 | 江苏省海洋水产研究所 | Method for inducing spawning of parent Solen grandis and collecting fertilized eggs |
| CN102100194A (en) * | 2010-12-02 | 2011-06-22 | 象山红升水产养殖有限公司 | Fry rearing method for sinonovacula constricta |
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| KR101304951B1 (en) * | 2011-10-17 | 2013-09-06 | 순천향대학교 산학협력단 | Seedling production technique in early out-of-season for korean bullhead |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1336108A (en) * | 2000-07-31 | 2002-02-20 | 山东省日照市水产研究所 | Artificial method for breeding giant razor cham |
| CN101731158A (en) * | 2008-11-24 | 2010-06-16 | 张明钰 | Shallow pond culture technique for sinonovacula constricta lamarck |
| CN101946737A (en) * | 2010-09-10 | 2011-01-19 | 江苏省海洋水产研究所 | Method for inducing spawning of parent Solen grandis and collecting fertilized eggs |
| CN102100194A (en) * | 2010-12-02 | 2011-06-22 | 象山红升水产养殖有限公司 | Fry rearing method for sinonovacula constricta |
Non-Patent Citations (1)
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| 缢蛏苗种繁育与池塘养殖高产技术;张玉胜等;《河北渔业》;20121231(第2期);第26-28页 * |
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