CN117652438A - Method for promoting copulation and spawning of Babylonia and application thereof - Google Patents
Method for promoting copulation and spawning of Babylonia and application thereof Download PDFInfo
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- CN117652438A CN117652438A CN202311463533.9A CN202311463533A CN117652438A CN 117652438 A CN117652438 A CN 117652438A CN 202311463533 A CN202311463533 A CN 202311463533A CN 117652438 A CN117652438 A CN 117652438A
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- 241000606434 Babylonia Species 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000001737 promoting effect Effects 0.000 title claims abstract description 26
- 230000027326 copulation Effects 0.000 title claims description 20
- 210000000582 semen Anatomy 0.000 claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 40
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 36
- 230000013011 mating Effects 0.000 claims abstract description 34
- 238000002791 soaking Methods 0.000 claims abstract description 29
- UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 241000237858 Gastropoda Species 0.000 claims abstract description 22
- 210000003250 oocyst Anatomy 0.000 claims abstract description 11
- 238000009395 breeding Methods 0.000 claims abstract description 8
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 230000017448 oviposition Effects 0.000 claims description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 241000237502 Ostreidae Species 0.000 claims description 12
- 235000020636 oyster Nutrition 0.000 claims description 12
- 241000238557 Decapoda Species 0.000 claims description 10
- 238000010992 reflux Methods 0.000 claims description 7
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 claims description 5
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000002386 leaching Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 210000001015 abdomen Anatomy 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 18
- 210000002149 gonad Anatomy 0.000 description 10
- 238000010586 diagram Methods 0.000 description 5
- 230000002710 gonadal effect Effects 0.000 description 5
- 238000001694 spray drying Methods 0.000 description 5
- 230000004584 weight gain Effects 0.000 description 5
- 235000019786 weight gain Nutrition 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 239000002932 luster Substances 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 235000015170 shellfish Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 241000237852 Mollusca Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000016087 ovulation Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000250967 Branchia Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000003688 hormone derivative Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/51—Culture of aquatic animals of shellfish of gastropods, e.g. abalones or turban snails
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/40—Peroxides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for promoting the mating and spawning of Babylonia. The method comprises the following steps: s1, selecting parent snails; s2, feeding baits: firstly, soaking baits in the semen astragali complanati extract, and then feeding the soaked baits 7-10 days before spawning by the Babylonia individuals in the step S1; the weight of the semen astragali complanati extract accounts for 0.1-0.3% of the weight of the bait; s3, full pool immersionAnd (3) soaking: stopping feeding baits before spawning of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking pentanediol or hydrogen peroxide in the whole pond; the dosage of the pentanediol or the hydrogen peroxide is 3-6 mL/m 3 The method comprises the steps of carrying out a first treatment on the surface of the S4, collecting oocysts after mating and spawning of parent snails. The method solves the bottleneck problem of low mating rate of the Babylonia parents, can effectively, conveniently and rapidly improve the mating rate, the breeding rate and the spawning quantity of the Babylonia parents without toxic or side effects, and greatly improves the economic benefit of propagation of the Babylonia.
Description
Technical Field
The invention belongs to the technical field of Babylonia cultivation, and particularly relates to a method for promoting copulation and spawning of Babylonia and application thereof.
Background
Babylonia belongs to the phylum Mollusca (Mollusca), class Gastropoda (Gastropoda), class pre-branchia (Prosablanchia), new Abdominal order (Neopatropoda), family Bucciidae (Bucciidae), genus Babylonia (Babylonia), and is distributed in tropical and subtropical areas, and the cultured varieties mainly comprise three species of Babylonia quadricarina, babylonia and Babylonia taiwan. Babylonia is a meat shallow seafloor shellfish, has delicious taste, and is a high-protein low-fat rare seafood meeting the modern nutritional requirements.
Babylonia mainly depends on sea water fishing to cause serious decay of natural resources, and the Babylonia can not meet the market demand by relying on natural shellfish fishing for a long time. Therefore, only through the research of artificial breeding technology, the artificial breeding of a large number of breeding seeds can meet a large number of demands of the market on the Babylonia, and meanwhile, the natural resource protection of the Babylonia is also positively acted.
At present, common methods for promoting gonad maturation of aquatic animals mainly comprise synthetic hormone, hormone analogue, animal-derived gonad or pituitary extract and the like, and methods such as heating and cooling, running water stimulation, drying in the shade and the like are commonly used in the shellfish field, but have no relevant report in the Babylonia field. Although the propagation technology of the Babylonia is mature after exploration for more than ten years, the problem of low mating rate of the Babylonia parent still exists. Therefore, the method for improving the mating rate and the spawning quantity of the Babylonia parents, which is effective, convenient and free from toxic and side effects, is explored and invented, and is a problem which needs to be solved currently and rapidly.
Disclosure of Invention
The invention aims at solving the prior art problems, and the primary aim of the invention is to provide a method for promoting the mating and spawning of the Babylonia, which can stimulate the mating and spawning of the Babylonia, promote the seminal emission and ovulation of the Babylonia, improve the quality and quantity of sperms, ova and oocysts, promote the mating and breeding of the Babylonia, improve the spawning quantity and further improve the economic benefit of the seedling raising of the Babylonia.
A second object of the present invention is to provide an application of the method for promoting copulation and oviposition of Babylonia in cultivation of Babylonia.
In order to achieve the above object, the present invention is realized by the following technical scheme:
a method for promoting the mating and oviposition of Babylonia, comprising the following steps:
s1, selecting parent snails: selecting Babylonia individuals to be spawned;
s2, feeding baits: firstly, soaking baits in the semen astragali complanati extract, and then feeding the soaked baits 7-10 days before spawning by the Babylonia individuals in the step S1; the weight of the semen astragali complanati extract accounts for 0.1-0.3% of the weight of the bait;
s3, soaking in a whole pool: stopping feeding baits before spawning of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking pentanediol or hydrogen peroxide in the whole pond; the dosage of the pentanediol or the hydrogen peroxide is 3-6 mL/m 3 ；
S4, collecting oocysts after mating and spawning of parent snails.
The inventor finds that the low copulation rate of the Babylonia is mainly related to the asynchronous sexual maturity of male and female. The female and male Babylonia are out of synchronization in sexual maturity, so that the male and female Babylonia are easy to cause unmatched, the spawning quantity is far insufficient in the full season of seedling cultivation, and the low mating rate of parent Babylonia is a main bottleneck for restricting the large-scale propagation and cultivation of Babylonia. In the invention, the semen astragali complanati extract has better effect of promoting semen generation and ovulation of the Babylonia, can improve the quality of the sperm, ovum and oocyst of the Babylonia and promote the premature gonad; the pentanediol or the hydrogen peroxide has the function of promoting mating and breeding and improving spawning amount when the gonad of the Babylonia parent is mature. Further, the inventors found that the amount of semen astragali Complanati extract, the amount of pentanediol or hydrogen peroxide was important for promoting copulation and oviposition of Babylonia. The use of the semen astragali complanati extract, when the use of pentanediol or hydrogen peroxide is small, is difficult to promote the Babylonia, and when the use of the semen astragali complanati extract, the use of the pentanediol or hydrogen peroxide is large, the Babylonia can produce an inhibiting effect on mating and spawning.
According to the invention, the special amount of semen astragali complanati extract is fed before oviposition of the Babylonia, and the Babylonia is soaked in a full pool by combining with the special amount of pentanediol or hydrogen peroxide, so that the weight and gonad maturity of the Babylonia are greatly improved, the oviposition amount and the oviposition rate of the Babylonia are also improved, and the mating oviposition time of the Babylonia is reduced.
Preferably, in the step S1, the eastern conch to be spawned is healthy, normal in pattern, free of damage to screw shells, full of soft body, and incapable of fully shrinking into the shells, and the size of the eastern conch is less than or equal to 40 heads/jin.
Preferably, in the step S2, the bait comprises 5-7 parts of crabs and 4-6 parts of oysters in parts by weight. More specifically, the crab is an egg-holding crab; the oyster characteristics are as follows: fresh, fat and beautiful, milky white soft part, black edge, luster and elasticity, and no extra juice when placed in water. More specifically, the bait is fed once in the evening at 6 to 7 points per day.
Preferably, in the step S2, the feeding amount of the bait accounts for 8-15% of the total weight of the Babylonia.
Preferably, in the step S2, the preparation method of the semen astragali complanati extract comprises the following steps: reflux extracting semen astragali Complanati with ethanol at 50-100deg.C, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain semen astragali Complanati extract.
Preferably, the concentration of the ethanol is 40-80%.
Preferably, the times of reflux extraction are 2-3 times, and leaching liquor obtained by the reflux extraction for a plurality of times is combined and then is subjected to suction filtration.
Preferably, the drying is spray drying, and the temperature of the spray drying is 50-80 ℃.
Preferably, in the step S3, the whole pool is soaked with pentanediol or hydrogen peroxide for 12 to 36 hours.
Preferably, the moisture content of the semen astragali complanati extract is 5-20%.
Preferably, in the step S4, during mating and spawning, the environmental conditions of the water area are as follows: the water temperature is 25-30 ℃, the pH value is 7.8-8.5, the ammonia nitrogen content is less than or equal to 0.05, and the nitrous acid content is less than or equal to 0.05.
Preferably, the Babylonia is one or more of Babylonia quadricarina, babylonia mud, and Babylonia taiwan.
Furthermore, the invention also claims the application of the method for promoting the copulation and spawning of the Babylonia in the cultivation of the Babylonia.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, the semen astragali complanati extract with a specific dosage is fed before oviposition of the Babylonia, and the Babylonia is soaked in a full pool by combining with the pentanediol or the hydrogen peroxide with a specific dosage, so that the bottleneck problem of low mating rate of the Babylonia parents in the current situation is solved, the mating rate, the propagation rate and the oviposition amount of the Babylonia parents can be effectively, conveniently and without toxic or side effects, the economic benefit of propagation of the Babylonia is greatly improved, and the green, healthy and sustainable development of the Babylonia breeding industry is facilitated. The method for cultivating the Babylonia can ensure that the spawning period of the Babylonia is short and concentrated, is favorable for collecting oocysts, ensures synchronous embryo development, and ensures that cultivated spiral seedlings are not irregular.
Drawings
FIG. 1 is a state diagram of the gonads when they are not developed.
Fig. 2 is a state diagram after gonadal development of male snails.
FIG. 3 is a state diagram after gonadal development of female snails.
FIG. 4 is a diagram of oviposition by mating parent snails.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Example 1 method for promoting copulation and oviposition of Babylonia
(1) Selecting parent snails: selecting an individual of Babylonia to be spawned (shown in figure 1), wherein the individual of Babylonia is healthy, has normal patterns, has no damage to screw shells, is full of soft body and cannot fully retract into the shells, and the size of the Babylonia is less than or equal to 40 heads/jin;
(2) Semen astragali Complanati extract is prepared by: cleaning semen astragali Complanati, cutting, pulverizing, reflux extracting with ethanol (60%) at 80deg.C for 2 times (each time for 1 hr), mixing the 2 times of extractive solutions, vacuum filtering, concentrating under reduced pressure to obtain extract, and spray drying at 65deg.C to water content of 15% to obtain semen astragali Complanati extract;
(3) Feeding baits: firstly, soaking baits in the semen astragali complanati extract, wherein the feeding amount of the baits accounts for 11.5 percent of the total weight of the semen astragali complanati extract (the baits comprise 6 parts of crabs and 5 parts of oysters, the crabs can select the oozed heterocrabs, the oysters can select the characteristics of freshness, fat meat, milky white soft part, black edge, luster and elasticity, and the oysters which can not float and sink in water and have redundant juice when placed in water), the weight of the semen astragali complanati extract accounts for 0.2 percent of the weight of the baits, and the semen Babylonicae individuals in the step (1) start feeding the soaked baits 7 to 10 days before spawning;
(4) Soaking in a full pool: stopping feeding baits before oviposition of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking pentanediol in the whole pond for 24 hours (the dosage of the pentanediol is 4.5 mL/m) 3 ) No water is changed during the soaking period, water is added and water is discharged when the soaking time is up, as shown in figures 2 and 3, which are respectively state diagrams after gonad development of male snails and female snails;
(5) During mating and spawning, the environmental conditions of the water area are as follows: the water temperature is 25-30 ℃, the pH value is 7.8-8.5, the ammonia nitrogen content is less than or equal to 0.05, the nitrous acid content is less than or equal to 0.05, and oocysts are collected after copulation and oviposition of parent snails (shown in figure 4).
Example 2 method for promoting copulation and oviposition of Babylonia
The difference between this embodiment and embodiment 1 is that: and (4) soaking in a full pool by adopting hydrogen peroxide.
Example 3 method for promoting copulation and oviposition of Babylonia
(1) Selecting parent snails: selecting an eastern conch individual to be spawned, wherein the eastern conch individual is healthy, has normal patterns, has no damage to a conch shell, is full of soft body and cannot fully retract into the shell, and the size of the eastern conch is less than or equal to 40 heads/jin;
(2) Semen astragali Complanati extract: cleaning semen astragali Complanati, cutting into segments, pulverizing, reflux-extracting with ethanol (60%) at 80deg.C for 3 times, each time for 1 hr, mixing the 3 times of extractive solutions, vacuum filtering, concentrating under reduced pressure to obtain extract, and spray-drying at 65deg.C until the water content is 15%, to obtain semen astragali Complanati extract;
(3) Feeding baits: firstly, soaking baits in semen astragali complanati extract, wherein the feeding amount of the baits accounts for 8 percent of the total weight of the Babylonia (the baits comprise 6 parts of crabs and 5 parts of oysters, the crabs can select to hold eggs and other crabs, the oysters can select to have the characteristics of freshness, fat meat, milky white soft parts, black edges, luster and elasticity, and can not float and sink in water when being put into water, and the oysters without redundant juice) and the weight of the semen astragali complanati extract accounts for 0.1 percent of the weight of the baits, and the Babylonia individual in the step (1) starts to feed the soaked baits 7 to 10 days before spawning;
(4) Soaking in a full pool: stopping feeding baits before oviposition of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking pentanediol in the whole pond for 36 hours (the dosage of the pentanediol is 3 mL/m) 3 ) No water is changed during the soaking period, and water is added and drained after the soaking time is up;
(5) During mating and spawning, the environmental conditions of the water area are as follows: the water temperature is 25-30 ℃, the pH value is 7.8-8.5, the ammonia nitrogen content is less than or equal to 0.05, the nitrous acid content is less than or equal to 0.05, and oocysts are collected after copulation and oviposition of parent snails.
Example 4 method for promoting copulation and oviposition of Babylonia
(1) Selecting parent snails: selecting an eastern conch individual to be spawned, wherein the eastern conch individual is healthy, has normal patterns, has no damage to a conch shell, is full of soft body and cannot fully retract into the shell, and the size of the eastern conch is less than or equal to 40 heads/jin;
(2) Semen astragali Complanati extract: cleaning semen astragali Complanati, cutting into segments, pulverizing, reflux-extracting with ethanol (60%) at 80deg.C for 3 times, each time for 1 hr, mixing the 3 times of extractive solutions, vacuum filtering, concentrating under reduced pressure to obtain extract, and spray-drying at 65deg.C until the water content is 15%, to obtain semen astragali Complanati extract;
(3) Feeding baits: firstly, soaking baits in semen astragali complanati extract, wherein the feeding amount of the baits accounts for 15 percent of the total weight of the Babylonia (the baits comprise 6 parts of crabs and 5 parts of oysters, the crabs can select to hold eggs and other crabs, the oysters can select to have the characteristics of freshness, fat meat, milky white soft parts, black edges, luster and elasticity, and can not float and sink in water when being put into water, and the oysters without redundant juice) and the weight of the semen astragali complanati extract accounts for 0.3 percent of the weight of the baits, and the Babylonia individual in the step (1) starts to feed the soaked baits 7-10 days before spawning;
(4) Soaking in a full pool: stopping feeding baits before oviposition of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking hydrogen peroxide in the whole pond for 36 hours (the hydrogen peroxide dosage is 6 mL/m) 3 ) No water is changed during the soaking period, and water is added and drained after the soaking time is up;
(5) During mating and spawning, the environmental conditions of the water area are as follows: the water temperature is 25-30 ℃, the pH value is 7.8-8.5, the ammonia nitrogen content is less than or equal to 0.05, the nitrous acid content is less than or equal to 0.05, and oocysts are collected after copulation and oviposition of parent snails.
Comparative example 1
The difference between this comparative example and example 1 is that: absence of step (2); in the step (3), the semen astragali Complanati extract is not used for soaking the bait.
Comparative example 2
The difference between this comparative example and example 1 is that: in the step (4), no pentanediol is adopted for full pool soaking.
Comparative example 3
The difference between this comparative example and example 1 is that: absence of step (2); in the step (3), the semen astragali Complanati extract is not used for soaking the bait; in the step (4), no pentanediol is adopted for full pool soaking.
Comparative example 4
The difference between this comparative example and example 1 is that: the dosage of pentanediol is 10mL/m 3 。
Comparative example 5
The difference between this comparative example and example 1 is that: the weight of semen astragali Complanati extract accounts for 1.0% of the weight of the bait.
Test example 1
The above examples and comparative examples were tested using the following methods:
weight gain rate: 30 cells were randomly sampled from each cell, the average of the individual weights was calculated, and the weight gain was obtained by subtracting the difference between the initial individual weights from the average and comparing the initial weights.
Gonadal maturity index: removing attached organisms and sediment on the surface of the spiral shell, breaking the spiral shell, taking out the soft body part, weighing the soft body part by an electronic balance, spirally shearing gonads along the liver by a scalpel, weighing, and sampling 10 samples each time.
Spawning rate: the egg-bag produced was weighed (spawning amount) and divided by the initial weight of all the snails in the pool to obtain the spawning rate.
Calculation formula of test data:
weight gain = (final weight-initial weight)/initial weight×100%;
gonadal maturity index GI (W) =gonadal weight/soft weight x 100%;
spawning rate = spawning amount/screw weight x 100%
Mating spawning period = spawning days + non-spawning days;
day of spawning interval = day of interval of spawning period to next spawning period;
number of oviposition concentration days = number of days in which oviposition amount is 90% or more;
the data of the above examples and comparative examples are shown in table 1 below.
TABLE 1
As is clear from Table 1, in examples 1 to 4, the weight gain of Babylonia was not less than 4.9%, and the gonad maturity index was not less than 9.2%. The spawning rate of parent snail mating spawning is more than or equal to 22 percent, the weight of the collected oocysts is more than or equal to 44 jin, and the mating spawning period, the spawning period interval days and the spawning concentration days are all obviously improved. Semen astragali Complanati extract is used at an amount of 0.1% and 3mL/m 3 The effect is best when the pentanediol is soaked in the whole pool for 36 hours, and the spawning amount is the largest.
Comparative example 1, comparative example 2 and comparative example 3 are comparative examples in which the semen astragali Complanati extract was not used, the pentanediol was not used, and the treatment with the semen astragali Complanati extract and the pentanediol was performed, respectively. From the data, the weight gain rate and gonad maturity index of the Babylonia are obviously reduced. The spawning rate of parent snail mating spawning is also obviously reduced, and the weight of collected oocysts is also obviously reduced. The mating spawning period, the spawning period interval days and the spawning concentration days are obviously prolonged.
Comparative examples 4 and 5 are comparative examples in which an excess of pentanediol was used and an excess of semen astragali complanati extract was used, and it was found from the data of examples 1, 4 and 5 that in the appropriate amount range, either semen astragali complanati extract or pentanediol or hydrogen peroxide could increase the gonad maturity of the parent of the Babylonia and promote mating and oviposition, and the effects could be superimposed by the cross use, but the excess could be suppressed instead, and the effect was reduced, but no discomfortable reaction was found in the parent.
The foregoing examples are illustrative only and serve to explain some features of the method of the invention. The claims that follow are intended to claim the broadest possible scope as conceivable and the embodiments presented herein are demonstrated for the applicant's true test results. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.
Claims (10)
1. A method for promoting the mating and oviposition of Babylonia, comprising the steps of:
s1, selecting parent snails: selecting Babylonia individuals to be spawned;
s2, feeding baits: firstly, soaking baits in the semen astragali complanati extract, and then feeding the soaked baits 7-10 days before spawning by the Babylonia individuals in the step S1; the weight of the semen astragali complanati extract accounts for 0.1-0.3% of the weight of the bait;
s3, soaking in a whole pool: stopping feeding baits before spawning of Babylonia, increasing the water exchange amount to 0.5-1 pond water/hour, and soaking pentanediol or hydrogen peroxide in the whole pond; the dosage of the pentanediol or the hydrogen peroxide is 3-6 mL/m 3 ；
S4, collecting oocysts after mating and spawning of parent snails.
2. The method for promoting the mating and spawning of the Babylonia according to claim 1, wherein in the step S1, the Babylonia individual to be spawned is healthy, normal in pattern, free of damage to screw shells, soft and full of fat, and the belly cannot fully retract into the shells, and the size of the Babylonia individual is less than or equal to 40 heads/jin.
3. The method for promoting the mating and spawning of Babylonia according to claim 1, wherein in the step S2, the bait comprises 5-7 parts by weight of crabs and 4-6 parts by weight of oysters.
4. The method for promoting the mating and oviposition of the Babylonia according to claim 1, wherein in the step S2, the feeding amount of the baits accounts for 8-15% of the total weight of the Babylonia.
5. The method for promoting copulation and spawning of Babylonia according to claim 1, wherein in the step S2, the preparation method of the semen astragali complanati extract is as follows: reflux extracting semen astragali Complanati with ethanol at 50-100deg.C, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain semen astragali Complanati extract.
6. The method for promoting copulation and oviposition of Babylonia according to claim 5, wherein the times of reflux leaching are 2-3 times, and leaching solutions obtained by the reflux leaching are combined and then subjected to suction filtration.
7. The method for promoting copulation and oviposition of Babylonia according to claim 5, wherein in the step S3, the whole pool is soaked with pentanediol or hydrogen peroxide for 12-36 hours.
8. The method for promoting copulation and spawning of Babylonia according to claim 1, wherein in the step S4, the environmental conditions of the water area are maintained during copulation and spawning as follows: the water temperature is 25-30 ℃, the pH value is 7.8-8.5, the ammonia nitrogen content is less than or equal to 0.05, and the nitrous acid content is less than or equal to 0.05.
9. The method of promoting mating and oviposition of Babylonia according to claim 1, wherein the Babylonia is one or more of Babylonia quadrangularis, babylonia muricata and Babylonia.
10. Use of the method for promoting copulation and spawning of Babylonia according to any of claims 1 to 9 in breeding of Babylonia.
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