CN100365127C - 具有遍在染色质开放表达元件(ucoe)的聚核苷酸 - Google Patents

具有遍在染色质开放表达元件(ucoe)的聚核苷酸 Download PDF

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CN100365127C
CN100365127C CNB998111554A CN99811155A CN100365127C CN 100365127 C CN100365127 C CN 100365127C CN B998111554 A CNB998111554 A CN B998111554A CN 99811155 A CN99811155 A CN 99811155A CN 100365127 C CN100365127 C CN 100365127C
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gene
expression
cell
sequence
polynucleotide
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Expired - Lifetime
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Chinese (zh)
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CN1334878A (zh
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迈克尔·安东尼乌
罗伯特·克龙比
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EMD Millipore Corp
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Cobra Therapeutics Ltd
Millipore Corp
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Priority claimed from GBGB9815879.3A external-priority patent/GB9815879D0/en
Priority claimed from GBGB9906712.6A external-priority patent/GB9906712D0/en
Priority claimed from GBGB9909494.8A external-priority patent/GB9909494D0/en
Application filed by Cobra Therapeutics Ltd, Millipore Corp filed Critical Cobra Therapeutics Ltd
Publication of CN1334878A publication Critical patent/CN1334878A/zh
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
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    • C12N2830/00—Vector systems having a special element relevant for transcription
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    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB998111554A 1998-07-21 1999-07-21 具有遍在染色质开放表达元件(ucoe)的聚核苷酸 Expired - Lifetime CN100365127C (zh)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
GB9815879.3 1998-07-21
GBGB9815879.3A GB9815879D0 (en) 1998-07-21 1998-07-21 A polynucleotide
US10768898P 1998-11-09 1998-11-09
US60/107,688 1998-11-09
GB9906712.6 1999-03-23
GBGB9906712.6A GB9906712D0 (en) 1999-03-23 1999-03-23 A polynucleotide
US12741099P 1999-04-01 1999-04-01
US60/127,410 1999-04-01
GB9909494.8 1999-04-23
GBGB9909494.8A GB9909494D0 (en) 1999-04-23 1999-04-23 A plasmid
US13401699P 1999-05-12 1999-05-12
US60/134,016 1999-05-12

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CNA2007101933472A Division CN101260384A (zh) 1998-07-21 1999-07-21 具有遍在染色质开放表达元件(ucoe)的聚核苷酸

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CN1334878A CN1334878A (zh) 2002-02-06
CN100365127C true CN100365127C (zh) 2008-01-30

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EP (2) EP1098986B1 (https=)
JP (4) JP4220673B2 (https=)
KR (3) KR100795626B1 (https=)
CN (1) CN100365127C (https=)
AU (1) AU771111B2 (https=)
CA (1) CA2333852C (https=)
MX (1) MXPA01000830A (https=)
WO (1) WO2000005393A2 (https=)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100996016B1 (ko) 2000-09-20 2010-11-22 밀리포어 코포레이션 폴리뉴클레오티드
DK1395669T3 (da) 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
ES2387951T3 (es) * 2001-04-05 2012-10-04 Emd Millipore Corporation Expresión génica mejorada
JP2005500019A (ja) * 2001-04-05 2005-01-06 エムエル ラボラトリーズ ピーエルシー 改良された遺伝子発現
US7812148B2 (en) 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
HUP0402659A3 (en) 2001-05-30 2008-07-28 Glaxo Group Ltd Chromosome-based platforms
KR20040032105A (ko) * 2001-06-04 2004-04-14 엠엘 래보러토리즈 피엘씨 재조합 단백질의 조성물 및 재조합 단백질의 고수준대규모 생산방법
PT1600510E (pt) 2001-07-04 2007-06-25 Chromagenics Bv Sequências de adn com actividade anti-repressora.
DE60329366D1 (de) 2002-01-09 2009-11-05 Univ Erasmus Medical Ct Induktierbare transposition beim transgenen organismus mit transposon-vektor
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
WO2003106674A2 (en) 2002-06-14 2003-12-24 Chromagenics B.V. Means and methods for regulating gene expression
ATE524559T1 (de) 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US9057079B2 (en) 2003-02-01 2015-06-16 Emd Millipore Corporation Expression elements
KR101269656B1 (ko) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
WO2005065348A2 (en) 2003-12-31 2005-07-21 Kalobios, Inc. Transactivation system for mammalian cells
EA014182B1 (ru) 2004-07-20 2010-10-29 Симфоген А/С КОМПОЗИЦИЯ ПОЛИКЛОНАЛЬНЫХ АНТИ-RhD АНТИТЕЛ, СПОСОБ ЕЁ ПОЛУЧЕНИЯ И ПРИМЕНЕНИЕ КОМПОЗИЦИИ
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
ES2384391T3 (es) 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
GB0504587D0 (en) 2005-03-05 2005-04-13 Ml Lab Plc Vectors comprising guinea pig CMV regulatory elements
GB0509965D0 (en) 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
WO2007081336A1 (en) * 2006-01-13 2007-07-19 Five Prime Therapeutics, Inc. Mammalian vectors for high-level expression of recombinant proteins
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
JP2010525789A (ja) * 2007-01-08 2010-07-29 ミリポア・コーポレイション １つ又はそれ以上のきょう雑物の低下したレベルの存在下で組換えタンパク質を生産するための細胞培養法
CN104531699B (zh) * 2014-10-09 2017-03-15 河南农业大学 一种增强外源基因表达的猪的ucoe调控元件片段
AU2015367290A1 (en) * 2014-12-16 2017-05-11 Garvan Institute Of Medical Research Sequencing controls
EP3601542A4 (en) * 2017-03-19 2021-01-13 Applied Stemcell, Inc. NEW INTEGRATION SITES AND THEIR USES
CA3056796A1 (en) 2017-04-03 2018-10-11 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for multiplexed quantitative analysis of cell lineages
US20200385754A1 (en) * 2017-12-07 2020-12-10 Integrity Biosolutions, Llc Engineered ubiquitous chromatin opening elements and uses thereof
US11781146B2 (en) * 2018-05-24 2023-10-10 National University Corporation Hokkaido University Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
SG11202100765UA (en) 2018-08-15 2021-03-30 Illumina Inc Compositions and methods for improving library enrichment
GB2592776B (en) * 2018-10-02 2023-08-16 Univ Leland Stanford Junior Compositions and methods for multiplexed quantitative analysis of cell lineages
WO2020223160A1 (en) * 2019-04-30 2020-11-05 Chan Zuckerberg Biohub, Inc. A novel chromatin-opening element for stable long term gene expression
CN112575031B (zh) * 2019-09-29 2023-04-07 新乡医学院 一种遍在染色质开放表达元件、重组表达载体、表达系统及其制备方法、应用
CN116574735B (zh) * 2023-07-03 2023-11-10 艾米能斯(苏州)生物科技有限公司 一种多核苷酸及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5166320A (en) 1987-04-22 1992-11-24 University Of Connecticut Carrier system and method for the introduction of genes into mammalian cells
GB8718779D0 (en) 1987-08-07 1987-09-16 Grosveld F G Dna sequence & expression vector
EP0945515A3 (en) 1989-11-06 2002-08-21 Cell Genesys, Inc. Production of proteins using homologous recombination
EP0532525B1 (de) 1990-05-18 1995-08-16 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Neue protein-polykation-konjugate
WO1991018088A1 (en) 1990-05-23 1991-11-28 The United States Of America, Represented By The Secretary, United States Department Of Commerce Adeno-associated virus (aav)-based eucaryotic vectors
GB9109928D0 (en) 1991-05-08 1991-07-03 Interox Chemicals Ltd Thickened compositions
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
CA2131620A1 (en) 1992-03-20 1993-09-30 Louis C. Smith A dna transporter system and method of use
US5583009A (en) 1992-12-08 1996-12-10 University Of Washington Method of preparing recombinant proteins in transgenic animals containing metallothionein gene elements that bestow tissue-independent copy number-dependent, position-indepedent gene expression
US5578475A (en) 1993-07-12 1996-11-26 Life Technologies, Inc. Composition and methods for transfecting eukaryotic cells
EP0831922A2 (en) 1995-06-08 1998-04-01 Therexsys Limited Improved pharmaceutical compositions for gene therapy
JP2000516472A (ja) 1996-08-16 2000-12-12 メディカル リサーチ カウンシル 組織特異的遺伝子発現を与える自己複製型エピソーム性発現ベクター
DE19651443A1 (de) * 1996-12-11 1998-06-18 Hoechst Ag Selbstverstärkende, pharmakologisch kontrollierbare Expressionssysteme
AU749113B2 (en) 1997-02-12 2002-06-20 Innovata Plc Compositions and methods for highly efficient transfection

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JP2009102331A (ja) 2009-05-14
KR100795626B1 (ko) 2008-01-17
EP1098986A2 (en) 2001-05-16
EP1098986B1 (en) 2011-12-28
AU5053499A (en) 2000-02-14
CA2333852A1 (en) 2000-02-03
KR20010083131A (ko) 2001-08-31
JP2008109931A (ja) 2008-05-15
WO2000005393A3 (en) 2000-08-17
CN1334878A (zh) 2002-02-06
WO2000005393A2 (en) 2000-02-03
JP2002522027A (ja) 2002-07-23
HK1035740A1 (en) 2001-12-07
JP4220673B2 (ja) 2009-02-04
EP2348118A1 (en) 2011-07-27
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