CN100365127C - 具有遍在染色质开放表达元件(ucoe)的聚核苷酸 - Google Patents

具有遍在染色质开放表达元件(ucoe)的聚核苷酸 Download PDF

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CN100365127C
CN100365127C CNB998111554A CN99811155A CN100365127C CN 100365127 C CN100365127 C CN 100365127C CN B998111554 A CNB998111554 A CN B998111554A CN 99811155 A CN99811155 A CN 99811155A CN 100365127 C CN100365127 C CN 100365127C
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gene
expression
cell
sequence
polynucleotide
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Expired - Lifetime
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CNB998111554A
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Chinese (zh)
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CN1334878A (zh
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迈克尔·安东尼乌
罗伯特·克龙比
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EMD Millipore Corp
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Cobra Therapeutics Ltd
Millipore Corp
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Priority claimed from GBGB9815879.3A external-priority patent/GB9815879D0/en
Priority claimed from GBGB9906712.6A external-priority patent/GB9906712D0/en
Priority claimed from GBGB9909494.8A external-priority patent/GB9909494D0/en
Application filed by Cobra Therapeutics Ltd, Millipore Corp filed Critical Cobra Therapeutics Ltd
Publication of CN1334878A publication Critical patent/CN1334878A/zh
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
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    • C12N2830/00—Vector systems having a special element relevant for transcription
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
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    • C12N2840/00—Vectors comprising a special translation-regulating system
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNB998111554A 1998-07-21 1999-07-21 具有遍在染色质开放表达元件(ucoe)的聚核苷酸 Expired - Lifetime CN100365127C (zh)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
GBGB9815879.3A GB9815879D0 (en) 1998-07-21 1998-07-21 A polynucleotide
GB9815879.3 1998-07-21
US10768898P 1998-11-09 1998-11-09
US60/107,688 1998-11-09
GBGB9906712.6A GB9906712D0 (en) 1999-03-23 1999-03-23 A polynucleotide
GB9906712.6 1999-03-23
US12741099P 1999-04-01 1999-04-01
US60/127,410 1999-04-01
GB9909494.8 1999-04-23
GBGB9909494.8A GB9909494D0 (en) 1999-04-23 1999-04-23 A plasmid
US13401699P 1999-05-12 1999-05-12
US60/134,016 1999-05-12

Related Child Applications (1)

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CNA2007101933472A Division CN101260384A (zh) 1998-07-21 1999-07-21 具有遍在染色质开放表达元件(ucoe)的聚核苷酸

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CN1334878A CN1334878A (zh) 2002-02-06
CN100365127C true CN100365127C (zh) 2008-01-30

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EP (2) EP2348118A1 (https=)
JP (4) JP4220673B2 (https=)
KR (3) KR100795626B1 (https=)
CN (1) CN100365127C (https=)
AU (1) AU771111B2 (https=)
CA (1) CA2333852C (https=)
MX (1) MXPA01000830A (https=)
WO (1) WO2000005393A2 (https=)

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US7812148B2 (en) 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
KR100955756B1 (ko) * 2001-04-05 2010-04-30 밀리포어 코포레이션 개량된 유전자 발현
EP2224010B1 (en) * 2001-04-05 2012-06-20 EMD Millipore Corporation Improved gene expression
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WO2002099070A2 (en) * 2001-06-04 2002-12-12 Corixa Corporation Compositions and methods for high-level, large-scale production of recombinant proteins
ATE466941T1 (de) 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor-aktivität
CA2470277A1 (en) 2002-01-09 2003-07-17 Minos Biosystems Limited Inducible transposition in transgenic organism using transposon vector
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
AU2003243059A1 (en) 2002-06-14 2003-12-31 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
EP1573058B1 (en) 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
ATE354645T1 (de) * 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
WO2004067701A2 (en) * 2003-02-01 2004-08-12 Innovata Plc Improved genetic elements providing high levels of expression
WO2005040377A2 (en) 2003-10-24 2005-05-06 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
WO2005065348A2 (en) 2003-12-31 2005-07-21 Kalobios, Inc. Transactivation system for mammalian cells
KR20070038556A (ko) 2004-07-20 2007-04-10 심포젠 에이/에스 항-ｒｈｅｓｕｓ ｄ 재조합 폴리클로날 항체 및 이의 제조방법
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US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060172382A1 (en) 2004-11-08 2006-08-03 Chromagenics B.V. Selection of host cells expressing protein at high levels
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GB0509965D0 (en) * 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
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CN104531699B (zh) * 2014-10-09 2017-03-15 河南农业大学 一种增强外源基因表达的猪的ucoe调控元件片段
KR102893385B1 (ko) * 2014-12-16 2025-12-02 가반 인스티튜트 오브 메디컬 리서치 시퀀싱 컨트롤
WO2018175153A1 (en) 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
CN110799643B (zh) 2017-04-03 2023-12-22 小利兰·斯坦福大学托管委员会 用于细胞谱系的多重定量分析的组合物和方法
US20200385754A1 (en) * 2017-12-07 2020-12-10 Integrity Biosolutions, Llc Engineered ubiquitous chromatin opening elements and uses thereof
CN112135909A (zh) * 2018-05-24 2020-12-25 国立大学法人北海道大学 新载体及其应用
AU2019320771B2 (en) * 2018-08-15 2025-12-18 Illumina Cambridge Limited Compositions and methods for improving library enrichment
AU2019354390A1 (en) * 2018-10-02 2021-04-01 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for multiplexed quantitative analysis of cell lineages
WO2020223160A1 (en) * 2019-04-30 2020-11-05 Chan Zuckerberg Biohub, Inc. A novel chromatin-opening element for stable long term gene expression
CN112575031B (zh) * 2019-09-29 2023-04-07 新乡医学院 一种遍在染色质开放表达元件、重组表达载体、表达系统及其制备方法、应用
CN116574735B (zh) * 2023-07-03 2023-11-10 艾米能斯(苏州)生物科技有限公司 一种多核苷酸及其应用

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AU5053499A (en) 2000-02-14
EP1098986A2 (en) 2001-05-16
WO2000005393A2 (en) 2000-02-03
WO2000005393A3 (en) 2000-08-17
KR100795626B1 (ko) 2008-01-17
CA2333852C (en) 2007-05-29
JP4220673B2 (ja) 2009-02-04
JP2008109931A (ja) 2008-05-15
KR20070108336A (ko) 2007-11-09
CN1334878A (zh) 2002-02-06
JP2002522027A (ja) 2002-07-23
KR20010083131A (ko) 2001-08-31
JP2005052149A (ja) 2005-03-03
KR20090117675A (ko) 2009-11-12
CA2333852A1 (en) 2000-02-03
JP2009102331A (ja) 2009-05-14
EP2348118A1 (en) 2011-07-27
AU771111B2 (en) 2004-03-11
MXPA01000830A (es) 2002-06-04
EP1098986B1 (en) 2011-12-28
HK1035740A1 (en) 2001-12-07

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