CN100336831C - 鲍鱼多糖的提取方法 - Google Patents
鲍鱼多糖的提取方法 Download PDFInfo
- Publication number
- CN100336831C CN100336831C CNB200510047409XA CN200510047409A CN100336831C CN 100336831 C CN100336831 C CN 100336831C CN B200510047409X A CNB200510047409X A CN B200510047409XA CN 200510047409 A CN200510047409 A CN 200510047409A CN 100336831 C CN100336831 C CN 100336831C
- Authority
- CN
- China
- Prior art keywords
- abalone
- minutes
- supernatant liquor
- polysaccharide
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 99
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 98
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 98
- 238000000034 method Methods 0.000 title claims abstract description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 98
- 102000004190 Enzymes Human genes 0.000 claims abstract description 97
- 108090000790 Enzymes Proteins 0.000 claims abstract description 97
- 238000005119 centrifugation Methods 0.000 claims abstract description 52
- 238000000605 extraction Methods 0.000 claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 230000003187 abdominal effect Effects 0.000 claims abstract description 18
- 238000001035 drying Methods 0.000 claims abstract description 16
- 210000001835 viscera Anatomy 0.000 claims abstract description 16
- 238000005507 spraying Methods 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 8
- 235000013372 meat Nutrition 0.000 claims abstract description 7
- 239000006228 supernatant Substances 0.000 claims description 92
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 58
- 238000001556 precipitation Methods 0.000 claims description 57
- 238000010792 warming Methods 0.000 claims description 32
- 239000002994 raw material Substances 0.000 claims description 31
- 238000000703 high-speed centrifugation Methods 0.000 claims description 21
- 210000002784 stomach Anatomy 0.000 claims description 21
- 230000007062 hydrolysis Effects 0.000 claims description 19
- 238000006460 hydrolysis reaction Methods 0.000 claims description 19
- 239000002002 slurry Substances 0.000 claims description 17
- 238000012546 transfer Methods 0.000 claims description 17
- 230000007935 neutral effect Effects 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 13
- 102000035092 Neutral proteases Human genes 0.000 claims description 12
- 108091005507 Neutral proteases Proteins 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 12
- 238000001816 cooling Methods 0.000 claims description 12
- 108090000145 Bacillolysin Proteins 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 9
- 238000002525 ultrasonication Methods 0.000 claims description 9
- 238000012545 processing Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 238000005360 mashing Methods 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 238000011160 research Methods 0.000 abstract description 8
- 102000004142 Trypsin Human genes 0.000 abstract description 7
- 108090000631 Trypsin Proteins 0.000 abstract description 7
- 239000003513 alkali Substances 0.000 abstract description 5
- 238000002137 ultrasound extraction Methods 0.000 abstract description 3
- 230000002358 autolytic effect Effects 0.000 abstract description 2
- 239000012588 trypsin Substances 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 abstract 7
- 239000000243 solution Substances 0.000 abstract 3
- 239000002131 composite material Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 102000057297 Pepsin A Human genes 0.000 abstract 1
- 108090000284 Pepsin A Proteins 0.000 abstract 1
- 229940111202 pepsin Drugs 0.000 abstract 1
- 150000004804 polysaccharides Polymers 0.000 abstract 1
- 239000010414 supernatant solution Substances 0.000 abstract 1
- 230000008569 process Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 17
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 16
- 241001465754 Metazoa Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 241000233805 Phoenix Species 0.000 description 1
- 241000206607 Porphyra umbilicalis Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004223 radioprotective effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Extraction Or Liquid Replacement (AREA)
- Medicines Containing Plant Substances (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510047409XA CN100336831C (zh) | 2005-10-11 | 2005-10-11 | 鲍鱼多糖的提取方法 |
| PCT/CN2006/002650 WO2007041951A1 (fr) | 2005-10-11 | 2006-10-10 | Procede d'extraction du polysaccharide de l'ormeau |
| JP2008533850A JP4420470B2 (ja) | 2005-10-11 | 2006-10-10 | あわび多糖類抽出方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200510047409XA CN100336831C (zh) | 2005-10-11 | 2005-10-11 | 鲍鱼多糖的提取方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1749280A CN1749280A (zh) | 2006-03-22 |
| CN100336831C true CN100336831C (zh) | 2007-09-12 |
Family
ID=36604952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200510047409XA Active CN100336831C (zh) | 2005-10-11 | 2005-10-11 | 鲍鱼多糖的提取方法 |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP4420470B2 (fr) |
| CN (1) | CN100336831C (fr) |
| WO (1) | WO2007041951A1 (fr) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101503721B (zh) * | 2008-11-27 | 2012-01-11 | 浙江珍世堂生物科技有限公司 | 一种蚌肉多糖的提取方法 |
| KR101257494B1 (ko) * | 2010-05-27 | 2013-04-26 | 조선대학교산학협력단 | 전복 소화 가수분해물을 함유하는 면역계 염증성 질환 예방 및 치료용 조성물 |
| CN101955548B (zh) * | 2010-09-26 | 2012-05-23 | 陕西农产品加工技术研究院 | 酶法辅助提取蒺藜多糖的方法 |
| CN102140141B (zh) * | 2011-05-13 | 2012-10-03 | 陈锦权 | 鲍鱼多糖的制备工艺 |
| CN102839149B (zh) * | 2012-09-21 | 2013-11-13 | 中国海洋大学 | 鲍鱼内脏多糖替代血清在细胞培养基中的应用 |
| CN103819575B (zh) * | 2014-03-07 | 2016-03-02 | 潍坊医学院 | 一种土鳖虫多糖的提取方法、提取的多糖以及该多糖的用途 |
| CN104381791B (zh) * | 2014-11-25 | 2015-08-26 | 烟台新海水产食品有限公司 | 一种速溶鲍鱼覆盆子颗粒冲剂及其制作方法 |
| CN104356249B (zh) * | 2014-11-27 | 2016-07-13 | 厦门蓝湾科技有限公司 | 一种高纯鲍鱼多糖的制备工艺及其应用 |
| CN104513843B (zh) * | 2014-12-30 | 2018-01-19 | 集美大学 | 一种多糖和蛋白肽的联合制备方法 |
| CN104497164A (zh) * | 2015-01-26 | 2015-04-08 | 吉林大学 | 高压脉冲电场辅助酶解提取河蚌多糖工艺 |
| CN105087713A (zh) * | 2015-09-01 | 2015-11-25 | 集美大学 | 鲍鱼脏器多糖的制备方法 |
| CN105272887B (zh) * | 2015-10-20 | 2017-06-23 | 集美大学 | 一种从鲍鱼内脏中同时提取牛磺酸和多糖的方法 |
| CN111513269B (zh) * | 2020-04-16 | 2022-07-19 | 自然资源部第三海洋研究所 | 一种鲍鱼内脏提取物及其应用 |
| CN113150178B (zh) * | 2021-04-14 | 2023-05-02 | 大连工业大学 | 一种硫酸化鲍鱼多糖及其在抑制新冠病毒中的应用 |
| CN114133459A (zh) * | 2021-11-23 | 2022-03-04 | 福建师范大学 | 一种鲍鱼多糖的制备方法及应用 |
| CN114805623A (zh) * | 2022-04-22 | 2022-07-29 | 福建大众健康生物科技有限公司 | 一种鲍鱼多糖的超低温冻融加压提取工艺 |
| CN115025515B (zh) * | 2022-06-10 | 2023-11-14 | 陈建 | 一种虎奶菇多糖提取分离设备 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1202749A (zh) * | 1997-04-11 | 1998-12-23 | 莫列斯公司 | 具有弹性啮合装置的输入/输出连接器 |
| CN1057776C (zh) * | 1997-06-27 | 2000-10-25 | 吉林大学 | 人参、西洋参多糖皂甙联合提取工艺 |
| CN1473508A (zh) * | 2003-08-07 | 2004-02-11 | 大连太平洋海龙科技有限公司 | 鲍鱼、海参、海胆营养液及其制造方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1202749C (zh) * | 2002-08-30 | 2005-05-25 | 大连轻工业学院 | 鲍鱼食品原液的制备方法 |
-
2005
- 2005-10-11 CN CNB200510047409XA patent/CN100336831C/zh active Active
-
2006
- 2006-10-10 WO PCT/CN2006/002650 patent/WO2007041951A1/fr active Application Filing
- 2006-10-10 JP JP2008533850A patent/JP4420470B2/ja active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1202749A (zh) * | 1997-04-11 | 1998-12-23 | 莫列斯公司 | 具有弹性啮合装置的输入/输出连接器 |
| CN1057776C (zh) * | 1997-06-27 | 2000-10-25 | 吉林大学 | 人参、西洋参多糖皂甙联合提取工艺 |
| CN1473508A (zh) * | 2003-08-07 | 2004-02-11 | 大连太平洋海龙科技有限公司 | 鲍鱼、海参、海胆营养液及其制造方法 |
Non-Patent Citations (2)
| Title |
|---|
| 鲍鱼多糖Hal-A的甲醇解研究 佘志刚 等，有机化学，第22卷第5期 2002 * |
| 鲍鱼多糖的提取及抗肿瘤试验 陈倩超，中国现代应用药学，第15卷第1期 1998 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP4420470B2 (ja) | 2010-02-24 |
| JP2009510234A (ja) | 2009-03-12 |
| WO2007041951A1 (fr) | 2007-04-19 |
| CN1749280A (zh) | 2006-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: DALIAN POLYTECHNIC COLLEGE; APPLICANT Free format text: FORMER OWNER: DALIAN POLYTECHNIC COLLEGE Effective date: 20060616 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20060616 Address after: 116034 No. 1 light industry garden, Ganjingzi District, Liaoning, Dalian Applicant after: Dalian Instutute of Light Industry Co-applicant after: Dalian Zhangzidao Fishery Group Co., Ltd. Address before: 116034 No. 1 light industry garden, Ganjingzi District, Liaoning, Dalian Applicant before: Dalian Instutute of Light Industry |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: DALIAN POLYTECHNIC UNIVERSITY Free format text: FORMER NAME: DALIAN LIGHT INDUSTRY COLLEGE |
|
| CP01 | Change in the name or title of a patent holder |
Address after: 116034 Ganjingzi Light Industry Zone, Liaoning City, No. 1 Co-patentee after: Dalian Zhangzidao Fishery Group Co., Ltd. Patentee after: Dalian Polytechnic University Address before: 116034 Ganjingzi Light Industry Zone, Liaoning City, No. 1 Co-patentee before: Dalian Zhangzidao Fishery Group Co., Ltd. Patentee before: Dalian Instutute of Light Industry |
|
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 116034 Ganjingzi Light Industry Zone, Liaoning City, No. 1 Patentee after: Dalian Polytechnic University Patentee after: Zhangzidao Island Group Co., Ltd. Address before: 116034 Ganjingzi Light Industry Zone, Liaoning City, No. 1 Patentee before: Dalian Polytechnic University Patentee before: Dalian Zhangzidao Fishery Group Co., Ltd. |