CA3203298A1 - Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogenes - Google Patents

Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogenes

Info

Publication number
CA3203298A1
CA3203298A1 CA3203298A CA3203298A CA3203298A1 CA 3203298 A1 CA3203298 A1 CA 3203298A1 CA 3203298 A CA3203298 A CA 3203298A CA 3203298 A CA3203298 A CA 3203298A CA 3203298 A1 CA3203298 A1 CA 3203298A1
Authority
CA
Canada
Prior art keywords
lamp
saliva
reaction
composition
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3203298A
Other languages
English (en)
Inventor
Jordan SEVILLE
Darby MCCHESNEY
Jiangshan WANG
Murali Kannan MARUTHAMUTHU
Andres DEXTRE
Mohit Verma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Raytheon BBN Technologies Corp
Purdue Research Foundation
Original Assignee
Raytheon BBN Technologies Corp
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Raytheon BBN Technologies Corp, Purdue Research Foundation filed Critical Raytheon BBN Technologies Corp
Publication of CA3203298A1 publication Critical patent/CA3203298A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente divulgation concerne des compositions et des procédés d'analyse d'amplification isotherme induite par boucle (LAMP, « loop-mediated isothermal amplification ») utilisant un signal de sortie dépendant du pH. La composition peut comprendre un colorant sensible au pH et une pluralité de réactifs LAMP non interférents. Le procédé peut comprendre la fourniture d'un ensemble d'un milieu en phase solide et d'une composition, le dépôt d'un échantillon biologique sur le milieu en phase solide et le chauffage de l'ensemble à une température isotherme suffisante pour faciliter une réaction LAMP.
CA3203298A 2021-01-15 2022-01-15 Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogenes Pending CA3203298A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15
US63/138,314 2021-01-15
PCT/US2022/012636 WO2022155547A1 (fr) 2021-01-15 2022-01-15 Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogènes

Publications (1)

Publication Number Publication Date
CA3203298A1 true CA3203298A1 (fr) 2022-07-21

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3203298A Pending CA3203298A1 (fr) 2021-01-15 2022-01-15 Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogenes

Country Status (12)

Country Link
EP (1) EP4277997A1 (fr)
JP (1) JP2024504297A (fr)
KR (1) KR20230126218A (fr)
CN (1) CN117917987A (fr)
AR (1) AR124657A1 (fr)
AU (1) AU2022207504A1 (fr)
BR (1) BR112023014267A2 (fr)
CA (1) CA3203298A1 (fr)
CL (1) CL2023002059A1 (fr)
MX (1) MX2023008417A (fr)
TW (1) TW202246523A (fr)
WO (1) WO2022155547A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016183012A1 (fr) * 2015-05-11 2016-11-17 3M Innovative Properties Company Composition visant à réduire l'inhibition de l'amplification d'acides nucléiques
EP4139456A4 (fr) * 2020-04-22 2024-05-22 President And Fellows Of Harvard College Procédés isothermes, compositions, kits et systèmes de détection d'acides nucléiques

Also Published As

Publication number Publication date
AU2022207504A1 (en) 2023-06-22
TW202246523A (zh) 2022-12-01
CL2023002059A1 (es) 2024-04-19
CN117917987A (zh) 2024-04-23
WO2022155547A1 (fr) 2022-07-21
BR112023014267A2 (pt) 2023-12-05
KR20230126218A (ko) 2023-08-29
EP4277997A1 (fr) 2023-11-22
JP2024504297A (ja) 2024-01-31
MX2023008417A (es) 2024-04-29
AR124657A1 (es) 2023-04-19

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