BR112023014267A2 - Análise de amplificação isotérmica mediada por loop (lamp) para alvos patogênicos - Google Patents

Análise de amplificação isotérmica mediada por loop (lamp) para alvos patogênicos

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Publication number
BR112023014267A2
BR112023014267A2 BR112023014267A BR112023014267A BR112023014267A2 BR 112023014267 A2 BR112023014267 A2 BR 112023014267A2 BR 112023014267 A BR112023014267 A BR 112023014267A BR 112023014267 A BR112023014267 A BR 112023014267A BR 112023014267 A2 BR112023014267 A2 BR 112023014267A2
Authority
BR
Brazil
Prior art keywords
lamp
loop
analysis
mediated
amplification
Prior art date
Application number
BR112023014267A
Other languages
English (en)
Inventor
Andres Dextre
Darby Mcchesney
Jiangshan Wang
Jordan Seville
Mohit Verma
Kannan Maruthamuthu Murali
Original Assignee
Purdue Research Foundation
Raytheon Bbn Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Raytheon Bbn Technologies Corp filed Critical Purdue Research Foundation
Publication of BR112023014267A2 publication Critical patent/BR112023014267A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

análise de amplificação isotérmica mediada por loop (lamp) para alvos patogênicos. a presente divulgação é voltada para composições e métodos para análise de amplificação isotérmica mediada por loop (lamp) utilizando um sinal de saída dependente de ph. a composição pode compreender um corante sensível ao ph e uma pluralidade de reagentes lamp não interferentes. o método pode compreender o fornecimento de uma montagem de um meio de fase sólida e uma composição, depositando uma amostra biológica no meio de fase sólida e aquecendo a montagem a uma temperatura isotérmica suficiente para facilitar uma reação lamp.
BR112023014267A 2021-01-15 2022-01-15 Análise de amplificação isotérmica mediada por loop (lamp) para alvos patogênicos BR112023014267A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15
PCT/US2022/012636 WO2022155547A1 (en) 2021-01-15 2022-01-15 Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets

Publications (1)

Publication Number Publication Date
BR112023014267A2 true BR112023014267A2 (pt) 2023-12-05

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112023014267A BR112023014267A2 (pt) 2021-01-15 2022-01-15 Análise de amplificação isotérmica mediada por loop (lamp) para alvos patogênicos

Country Status (12)

Country Link
EP (1) EP4277997A1 (pt)
JP (1) JP2024504297A (pt)
KR (1) KR20230126218A (pt)
CN (1) CN117917987A (pt)
AR (1) AR124657A1 (pt)
AU (1) AU2022207504A1 (pt)
BR (1) BR112023014267A2 (pt)
CA (1) CA3203298A1 (pt)
CL (1) CL2023002059A1 (pt)
MX (1) MX2023008417A (pt)
TW (1) TW202246523A (pt)
WO (1) WO2022155547A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3294905B1 (en) * 2015-05-11 2021-06-23 3M Innovative Properties Company Composition for reducing inhibition of nucleic acid amplification
CA3176545A1 (en) * 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids

Also Published As

Publication number Publication date
AR124657A1 (es) 2023-04-19
CA3203298A1 (en) 2022-07-21
EP4277997A1 (en) 2023-11-22
AU2022207504A1 (en) 2023-06-22
TW202246523A (zh) 2022-12-01
WO2022155547A1 (en) 2022-07-21
CN117917987A (zh) 2024-04-23
MX2023008417A (es) 2024-04-29
KR20230126218A (ko) 2023-08-29
JP2024504297A (ja) 2024-01-31
CL2023002059A1 (es) 2024-04-19

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