CL2023002059A1 - Análisis de amplificación isotérmica mediada por bucle (lamp) para objetivos patógenos - Google Patents

Análisis de amplificación isotérmica mediada por bucle (lamp) para objetivos patógenos

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Publication number
CL2023002059A1
CL2023002059A1 CL2023002059A CL2023002059A CL2023002059A1 CL 2023002059 A1 CL2023002059 A1 CL 2023002059A1 CL 2023002059 A CL2023002059 A CL 2023002059A CL 2023002059 A CL2023002059 A CL 2023002059A CL 2023002059 A1 CL2023002059 A1 CL 2023002059A1
Authority
CL
Chile
Prior art keywords
lamp
loop
analysis
isothermal amplification
mediated isothermal
Prior art date
Application number
CL2023002059A
Other languages
English (en)
Inventor
Jordan Seville
Darby Mcchesney
Jiangshan Wang
Murali Kannan Maruthamuthu
Andres Dextre
Mohit Verma
Original Assignee
Purdue Research Foundation
Raytheon Bbn Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Raytheon Bbn Technologies Corp filed Critical Purdue Research Foundation
Publication of CL2023002059A1 publication Critical patent/CL2023002059A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La presente descripción se relaciona con composiciones y métodos para análisis de amplificación isotérmica mediada por bucle (LAMP) usando una señal de salida dependiente del pH. La composición puede comprender un tinte sensible al pH y una pluralidad de reactivos de LAMP que no interfieren. El método puede comprender suministrar un montaje de un medio de fase sólida y una composición, depositar una muestra biológica sobre el medio de fase sólida y calentar el montaje hasta una temperatura isotérmica, suficiente para facilitar una reacción de LAMP.
CL2023002059A 2021-01-15 2023-07-14 Análisis de amplificación isotérmica mediada por bucle (lamp) para objetivos patógenos CL2023002059A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15

Publications (1)

Publication Number Publication Date
CL2023002059A1 true CL2023002059A1 (es) 2024-04-19

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2023002059A CL2023002059A1 (es) 2021-01-15 2023-07-14 Análisis de amplificación isotérmica mediada por bucle (lamp) para objetivos patógenos

Country Status (12)

Country Link
EP (1) EP4277997A1 (es)
JP (1) JP2024504297A (es)
KR (1) KR20230126218A (es)
CN (1) CN117917987A (es)
AR (1) AR124657A1 (es)
AU (1) AU2022207504A1 (es)
BR (1) BR112023014267A2 (es)
CA (1) CA3203298A1 (es)
CL (1) CL2023002059A1 (es)
MX (1) MX2023008417A (es)
TW (1) TW202246523A (es)
WO (1) WO2022155547A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017024191B1 (pt) * 2015-05-11 2024-01-02 3M Innovative Properties Company Composição aquosa, método de amplificação de ácido nucleico e kit
EP4139456A4 (en) * 2020-04-22 2024-05-22 President And Fellows Of Harvard College ISOTHERMAL METHODS, COMPOSITIONS, KITS AND SYSTEMS FOR THE DETECTION OF NUCLEIC ACIDS

Also Published As

Publication number Publication date
CN117917987A (zh) 2024-04-23
MX2023008417A (es) 2024-04-29
EP4277997A1 (en) 2023-11-22
WO2022155547A1 (en) 2022-07-21
CA3203298A1 (en) 2022-07-21
BR112023014267A2 (pt) 2023-12-05
AU2022207504A1 (en) 2023-06-22
TW202246523A (zh) 2022-12-01
AR124657A1 (es) 2023-04-19
JP2024504297A (ja) 2024-01-31
KR20230126218A (ko) 2023-08-29

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