AR124657A1 - Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos - Google Patents

Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos

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Publication number
AR124657A1
AR124657A1 ARP220100081A ARP220100081A AR124657A1 AR 124657 A1 AR124657 A1 AR 124657A1 AR P220100081 A ARP220100081 A AR P220100081A AR P220100081 A ARP220100081 A AR P220100081A AR 124657 A1 AR124657 A1 AR 124657A1
Authority
AR
Argentina
Prior art keywords
lamp
mediated isothermal
magnification
fork
analysis
Prior art date
Application number
ARP220100081A
Other languages
English (en)
Inventor
Jordan Seville
Darby Mchesney
Jiangshan Wang
Murali Kannan Maruthamuthu
Andres Dextre
Mohit Verma
Original Assignee
Purdue Research Foundation
Raytheon Bbn Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation, Raytheon Bbn Technologies Corp filed Critical Purdue Research Foundation
Publication of AR124657A1 publication Critical patent/AR124657A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La presente divulgación se refiere a composiciones y métodos para el análisis de amplificación isotérmica mediada por horquillas (LAMP) que utilizan una señal de salida dependiente del pH. La composición puede comprender un colorante sensible al pH y una pluralidad de reactivos LAMP no interferentes. El método puede comprender proporcionar un conjunto de un medio en fase sólida y una composición, depositar una muestra biológica en el medio en fase sólida y calentar el conjunto a una temperatura isotérmica suficiente para facilitar una reacción de LAMP.
ARP220100081A 2021-01-15 2022-01-17 Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos AR124657A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15

Publications (1)

Publication Number Publication Date
AR124657A1 true AR124657A1 (es) 2023-04-19

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220100081A AR124657A1 (es) 2021-01-15 2022-01-17 Análisis de amplificación isotérmica mediada por horquillas (lamp) para objetivos patógenos

Country Status (12)

Country Link
EP (1) EP4277997A1 (es)
JP (1) JP2024504297A (es)
KR (1) KR20230126218A (es)
CN (1) CN117917987A (es)
AR (1) AR124657A1 (es)
AU (1) AU2022207504A1 (es)
BR (1) BR112023014267A2 (es)
CA (1) CA3203298A1 (es)
CL (1) CL2023002059A1 (es)
MX (1) MX2023008417A (es)
TW (1) TW202246523A (es)
WO (1) WO2022155547A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102630602B1 (ko) * 2015-05-11 2024-01-26 쓰리엠 이노베이티브 프로퍼티즈 컴파니 핵산 증폭의 저해를 감소시키는 조성물
KR20230002943A9 (ko) * 2020-04-22 2024-04-26 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 검출을 위한 등온 방법, 조성물, 키트, 및 시스템

Also Published As

Publication number Publication date
MX2023008417A (es) 2024-04-29
AU2022207504A1 (en) 2023-06-22
CL2023002059A1 (es) 2024-04-19
KR20230126218A (ko) 2023-08-29
EP4277997A1 (en) 2023-11-22
JP2024504297A (ja) 2024-01-31
WO2022155547A1 (en) 2022-07-21
CA3203298A1 (en) 2022-07-21
TW202246523A (zh) 2022-12-01
CN117917987A (zh) 2024-04-23
BR112023014267A2 (pt) 2023-12-05

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