CN117917987A - 病原目标的环介导等温扩增(lamp)分析 - Google Patents

病原目标的环介导等温扩增(lamp)分析 Download PDF

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Publication number
CN117917987A
CN117917987A CN202280021435.7A CN202280021435A CN117917987A CN 117917987 A CN117917987 A CN 117917987A CN 202280021435 A CN202280021435 A CN 202280021435A CN 117917987 A CN117917987 A CN 117917987A
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CN
China
Prior art keywords
lamp
saliva
reaction
composition
concentration
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Pending
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CN202280021435.7A
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English (en)
Chinese (zh)
Inventor
J·塞维利亚
D·麦克切斯尼
J·王
M·K·玛鲁塔木图
A·德克斯特
M·维尔马
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Raytheon BBN Technologies Corp
Purdue Research Foundation
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Raytheon BBN Technologies Corp
Purdue Research Foundation
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Application filed by Raytheon BBN Technologies Corp, Purdue Research Foundation filed Critical Raytheon BBN Technologies Corp
Publication of CN117917987A publication Critical patent/CN117917987A/zh
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/301Hairpin oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN202280021435.7A 2021-01-15 2022-01-15 病原目标的环介导等温扩增(lamp)分析 Pending CN117917987A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15
US63/138,314 2021-01-15
PCT/US2022/012636 WO2022155547A1 (fr) 2021-01-15 2022-01-15 Analyse d'amplification isotherme induite par boucle (lamp) pour cibles pathogènes

Publications (1)

Publication Number Publication Date
CN117917987A true CN117917987A (zh) 2024-04-23

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280021435.7A Pending CN117917987A (zh) 2021-01-15 2022-01-15 病原目标的环介导等温扩增(lamp)分析

Country Status (12)

Country Link
EP (1) EP4277997A1 (fr)
JP (1) JP2024504297A (fr)
KR (1) KR20230126218A (fr)
CN (1) CN117917987A (fr)
AR (1) AR124657A1 (fr)
AU (1) AU2022207504A1 (fr)
BR (1) BR112023014267A2 (fr)
CA (1) CA3203298A1 (fr)
CL (1) CL2023002059A1 (fr)
MX (1) MX2023008417A (fr)
TW (1) TW202246523A (fr)
WO (1) WO2022155547A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017024191B1 (pt) * 2015-05-11 2024-01-02 3M Innovative Properties Company Composição aquosa, método de amplificação de ácido nucleico e kit
US20230203567A1 (en) * 2020-04-22 2023-06-29 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids

Also Published As

Publication number Publication date
AR124657A1 (es) 2023-04-19
BR112023014267A2 (pt) 2023-12-05
TW202246523A (zh) 2022-12-01
EP4277997A1 (fr) 2023-11-22
CL2023002059A1 (es) 2024-04-19
KR20230126218A (ko) 2023-08-29
AU2022207504A1 (en) 2023-06-22
AU2022207504A9 (en) 2025-01-16
WO2022155547A1 (fr) 2022-07-21
JP2024504297A (ja) 2024-01-31
MX2023008417A (es) 2024-04-29
CA3203298A1 (fr) 2022-07-21

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