TW202246523A - 用於病原目標之環介導恆溫擴增(lamp)分析 - Google Patents

用於病原目標之環介導恆溫擴增(lamp)分析 Download PDF

Info

Publication number
TW202246523A
TW202246523A TW111101753A TW111101753A TW202246523A TW 202246523 A TW202246523 A TW 202246523A TW 111101753 A TW111101753 A TW 111101753A TW 111101753 A TW111101753 A TW 111101753A TW 202246523 A TW202246523 A TW 202246523A
Authority
TW
Taiwan
Prior art keywords
lamp
saliva
reaction
composition
concentration
Prior art date
Application number
TW111101753A
Other languages
English (en)
Chinese (zh)
Inventor
喬登 塞維利亞
達比 麥克切斯尼
江山 王
穆拉利 K 馬魯塔穆圖
安德烈斯 德克斯特
莫希特 維爾瑪
Original Assignee
普渡研究基金會
美商雷神Bbn技術公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 普渡研究基金會, 美商雷神Bbn技術公司 filed Critical 普渡研究基金會
Publication of TW202246523A publication Critical patent/TW202246523A/zh

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/301Hairpin oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
TW111101753A 2021-01-15 2022-01-14 用於病原目標之環介導恆溫擴增(lamp)分析 TW202246523A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163138314P 2021-01-15 2021-01-15
US63/138,314 2021-01-15

Publications (1)

Publication Number Publication Date
TW202246523A true TW202246523A (zh) 2022-12-01

Family

ID=80222534

Family Applications (1)

Application Number Title Priority Date Filing Date
TW111101753A TW202246523A (zh) 2021-01-15 2022-01-14 用於病原目標之環介導恆溫擴增(lamp)分析

Country Status (12)

Country Link
EP (1) EP4277997A1 (fr)
JP (1) JP2024504297A (fr)
KR (1) KR20230126218A (fr)
CN (1) CN117917987A (fr)
AR (1) AR124657A1 (fr)
AU (1) AU2022207504A1 (fr)
BR (1) BR112023014267A2 (fr)
CA (1) CA3203298A1 (fr)
CL (1) CL2023002059A1 (fr)
MX (1) MX2023008417A (fr)
TW (1) TW202246523A (fr)
WO (1) WO2022155547A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116042785B (zh) * 2023-03-22 2023-07-14 翌圣生物科技(上海)股份有限公司 用于rt-lamp扩增试剂的缓冲剂及rt-lamp扩增试剂

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017024191B1 (pt) * 2015-05-11 2024-01-02 3M Innovative Properties Company Composição aquosa, método de amplificação de ácido nucleico e kit
US20230203567A1 (en) * 2020-04-22 2023-06-29 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids

Also Published As

Publication number Publication date
AR124657A1 (es) 2023-04-19
BR112023014267A2 (pt) 2023-12-05
EP4277997A1 (fr) 2023-11-22
CL2023002059A1 (es) 2024-04-19
KR20230126218A (ko) 2023-08-29
AU2022207504A1 (en) 2023-06-22
AU2022207504A9 (en) 2025-01-16
WO2022155547A1 (fr) 2022-07-21
CN117917987A (zh) 2024-04-23
JP2024504297A (ja) 2024-01-31
MX2023008417A (es) 2024-04-29
CA3203298A1 (fr) 2022-07-21

Similar Documents

Publication Publication Date Title
Miyamoto et al. Method for colorimetric detection of double-stranded nucleic acid using leuco triphenylmethane dyes
US20220228226A1 (en) Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets
Nörz et al. Pushing beyond specifications: evaluation of linearity and clinical performance of the cobas 6800/8800 SARS-CoV-2 RT-PCR assay for reliable quantification in blood and other materials outside recommendations
TW202246523A (zh) 用於病原目標之環介導恆溫擴增(lamp)分析
US20220228205A1 (en) Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets
Prado et al. Development and evaluation of a lyophilization protocol for colorimetric RT-LAMP diagnostic assay for COVID-19
TW202246522A (zh) 用於病原目標之環介導恆溫擴增(lamp)分析
TW202242131A (zh) 用於病原目標之環介導恆溫擴增(lamp)分析
JP2022153338A (ja) 核酸増幅用組成物の保存安定性の向上方法
US20230014132A1 (en) Loop-mediated isothermal amplification (lamp) analysis for pathogenic targets
KR20140006878A (ko) 핵산 변형 방법
CA3171097A1 (fr) Dosages et methodes de detection d'acides nucleiques viraux
US20240093278A1 (en) Rapid molecular diagnostics with unified-one-pot sample processing, nucleic acid amplification, and result readout
TR2023008291T2 (tr) Patojeni̇k hedefler i̇çi̇n döngü aracili i̇zotermal ampli̇fi̇kasyon (lamp) anali̇zi̇
Mao et al. CRISPR/Cas13a analysis based on NASBA amplification for norovirus detection
JPS59140899A (ja) オキシダ−ゼによる基質の新規定量法
RU2769572C1 (ru) Тест-система для выявления специфических нуклеотидных последовательностей, характерных для выявляемых микроорганизмов или вирусов, способ применения тест-системы (варианты)
Taglia et al. Development of a microwave-based extraction for forensic biological samples
TW202238128A (zh) 於固相介質上的環介導恆溫擴增(lamp)
TW202246524A (zh) 於固相介質上的環介導恆溫擴增(lamp)
TW202244279A (zh) 於固相介質上的環介導恆溫擴增(lamp)
TR2023008289T2 (tr) Loop-mediated isothermal amplification (lamp) on a solid-phase medium
WO2022082016A1 (fr) Lignes de régulation de débit pour dosages à écoulement latéral