CA3081113A1 - Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine - Google Patents

Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine Download PDF

Info

Publication number
CA3081113A1
CA3081113A1 CA3081113A CA3081113A CA3081113A1 CA 3081113 A1 CA3081113 A1 CA 3081113A1 CA 3081113 A CA3081113 A CA 3081113A CA 3081113 A CA3081113 A CA 3081113A CA 3081113 A1 CA3081113 A1 CA 3081113A1
Authority
CA
Canada
Prior art keywords
bacterium
recombinant bacterium
expression
encoding gene
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3081113A
Other languages
English (en)
French (fr)
Inventor
Tingyi WEN
Chen Zhang
Xiuling SHANG
Xin CHAI
Yun Zhang
Shuwen Liu
Guoqiang Wang
Zhongcai LI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Microbiology of CAS
Ningxia Eppen Biotech Co Ltd
Original Assignee
Institute of Microbiology of CAS
Ningxia Eppen Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Microbiology of CAS, Ningxia Eppen Biotech Co Ltd filed Critical Institute of Microbiology of CAS
Publication of CA3081113A1 publication Critical patent/CA3081113A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/78—Hansenula
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3081113A 2017-11-01 2018-05-22 Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine Pending CA3081113A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201711058221.4 2017-11-01
CN201711058221.4A CN109750069B (zh) 2017-11-01 2017-11-01 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法
PCT/CN2018/087894 WO2019085445A1 (zh) 2017-11-01 2018-05-22 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法

Publications (1)

Publication Number Publication Date
CA3081113A1 true CA3081113A1 (en) 2019-05-09

Family

ID=66331288

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3081113A Pending CA3081113A1 (en) 2017-11-01 2018-05-22 Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine

Country Status (7)

Country Link
US (1) US20200347419A1 (enExample)
EP (1) EP3690027A4 (enExample)
JP (1) JP6961819B2 (enExample)
CN (1) CN109750069B (enExample)
CA (1) CA3081113A1 (enExample)
PH (1) PH12020550529A1 (enExample)
WO (1) WO2019085445A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110256535B (zh) * 2019-06-14 2021-01-01 中国石油大学(华东) L-天门冬酰胺酶XiDL及其编码基因和应用
CA3144631A1 (en) * 2019-06-24 2020-12-30 Danisco Us Inc Disruption of cdc42 effectors in yeast for increased alcohol and lysine production
CN110592109B (zh) * 2019-08-28 2020-10-09 黑龙江伊品生物科技有限公司 一种spoT基因改造的重组菌株及其构建方法与应用
CN112877269B (zh) 2020-01-15 2021-12-24 中国科学院天津工业生物技术研究所 生产赖氨酸的微生物以及赖氨酸的生产方法
CN111607608B (zh) * 2020-04-20 2023-04-14 天津科技大学 基因工程高产菌株淀粉酶产色链霉菌及提高ε-聚赖氨酸产量的方法和应用
CN111850010B (zh) * 2020-06-08 2021-04-09 黑龙江伊品生物科技有限公司 一种dapB基因改造的重组菌株及其构建方法与应用
CN111979165B (zh) * 2020-08-07 2021-05-07 黑龙江伊品生物科技有限公司 一种产l-赖氨酸的重组菌株及其构建方法与应用
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
CN112301068B (zh) * 2020-10-29 2022-10-04 齐齐哈尔龙江阜丰生物科技有限公司 一种葡萄糖母液水解综合利用工艺
BR112023018219A2 (pt) * 2021-03-09 2023-11-28 Daesang Corp Variante de corynebacterium glutamicum que tem capacidade de produção de l-lisina aprimorada e método para produzir l-lisina com o uso da mesma
CN112695036B (zh) * 2021-03-23 2021-07-06 中国科学院天津工业生物技术研究所 一种天冬氨酸激酶基因表达调控序列及其应用
AU2021442398B2 (en) * 2021-04-29 2025-05-15 Cj Cheiljedang Corporation Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine using same
JP7683041B2 (ja) * 2021-04-30 2025-05-26 シージェイ チェイルジェダング コーポレイション Ｌ－リシン生産能が向上したコリネバクテリウム・グルタミカム変異株及びそれを用いたｌ－リシンの生産方法
CN113278571A (zh) * 2021-05-27 2021-08-20 齐鲁工业大学 一种棒状杆菌工程菌构建方法及应用
CN115449519B (zh) * 2021-06-08 2023-04-07 中国科学院天津工业生物技术研究所 基于dapB基因的具有启动子活性的多核苷酸及其用途
CN116179586A (zh) * 2021-11-26 2023-05-30 上海凯赛生物技术股份有限公司 用于发酵生产l-赖氨酸的表达盒、菌株及其应用
CN114908027B (zh) * 2022-01-20 2023-12-26 天津科技大学 泛酸生产相关菌株及其构建方法与应用
CN116004495B (zh) * 2022-10-12 2025-12-16 浙江工业大学 生产β-丙氨酸的重组基因工程菌及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2383865A1 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
DE102004035065A1 (de) * 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
US8420375B2 (en) * 2009-06-10 2013-04-16 Genomatica, Inc. Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol
EP2582815B1 (en) * 2010-06-15 2016-08-10 Daesang Corp. Production process for amino acids of the aspartate family using microorganisms
CA2802126A1 (en) * 2010-07-14 2012-01-19 Nestec S.A. Asparaginase from basidiomycetes
CN105734004B (zh) * 2016-03-02 2020-06-19 廊坊梅花生物技术开发有限公司 重组菌株及其制备方法、应用

Also Published As

Publication number Publication date
JP2021500914A (ja) 2021-01-14
EP3690027A1 (en) 2020-08-05
WO2019085445A1 (zh) 2019-05-09
CN109750069A (zh) 2019-05-14
PH12020550529A1 (en) 2021-04-26
CN109750069B (zh) 2025-08-01
US20200347419A1 (en) 2020-11-05
JP6961819B2 (ja) 2021-11-05
EP3690027A4 (en) 2020-11-11

Similar Documents

Publication Publication Date Title
CA3081113A1 (en) Recombinant bacterium capable of producing l-lysine, construction method thereof and production method of l-lysine
Du et al. Engineering Halomonas bluephagenesis for L-Threonine production
JP6188930B2 (ja) 産ｌ−アミノ酸の組換え菌およびその構築方法
JP2021500914A5 (enExample)
CN103396976B (zh) L-谷氨酸类氨基酸生产微生物以及氨基酸的生产方法
TWI583790B (zh) 具有產生ｌ－精胺酸能力的棒狀桿菌屬微生物及使用該微生物產生ｌ－精胺酸的方法
Ikeda Lysine fermentation: history and genome breeding
EP2824186B1 (en) L-lysine generation method by fermenting bacteria having modified aconitase gene and/or regulatory element
TW201712117A (zh) 用於製造腐胺或鳥胺酸之微生物及使用該微生物製造腐胺或鳥胺酸之方法
CN103555721A (zh) 改进的启动子和用其产生l-赖氨酸的方法
TW201718863A (zh) 用於製造腐胺或鳥胺酸的微生物及使用該微生物用於製造腐胺或鳥胺酸的方法
CN104619852B (zh) 用改变乌头酸酶基因和/或其调控元件的细菌发酵生产l-赖氨酸的方法
CN110117568B (zh) 生产l-组氨酸的重组菌、其构建方法以及l-组氨酸的生产方法
KR20200010218A (ko) Crm197의 높은 수준의 생산을 위한 개선된 방법
EP4538375A1 (en) Ep6 promoter, related biomaterial, and use thereof
US11028384B2 (en) Pyruvate carboxylase and pyruvate carboxylase-encoding DNA, plasmid containing said DNA and microorganism for the production thereof, and methods for the production of products the biosynthesis of which includes oxaloacetate as precursor, and chromosome
Liu et al. Coculture Corynebacterium glutamicum and Bacillus licheniformis for producing poly-γ-glutamic acid from glucose
US20150118720A1 (en) Process for producing amino acid
CN119120333A (zh) 一种重组微生物及其在生产l-异亮氨酸中的应用
CN119120516A (zh) 发酵生产维生素b5的工程菌及应用
WO2023142881A1 (zh) 产苏氨酸菌株的构建方法
Jakobsen Study and engineering of methanolassimilation and L-lysineproduction in the thermotolerantbacterium Bacillus methanolicus

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430

EEER Examination request

Effective date: 20200430