JP2021500914A5 - - Google Patents
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- JP2021500914A5 JP2021500914A5 JP2020524437A JP2020524437A JP2021500914A5 JP 2021500914 A5 JP2021500914 A5 JP 2021500914A5 JP 2020524437 A JP2020524437 A JP 2020524437A JP 2020524437 A JP2020524437 A JP 2020524437A JP 2021500914 A5 JP2021500914 A5 JP 2021500914A5
- Authority
- JP
- Japan
- Prior art keywords
- gene
- corynebacterium
- lysine
- bacterium
- recombinant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711058221.4A CN109750069B (zh) | 2017-11-01 | 2017-11-01 | 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法 |
| CN201711058221.4 | 2017-11-01 | ||
| PCT/CN2018/087894 WO2019085445A1 (zh) | 2017-11-01 | 2018-05-22 | 生产l-赖氨酸的重组菌、其构建方法以及l-赖氨酸的生产方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021500914A JP2021500914A (ja) | 2021-01-14 |
| JP2021500914A5 true JP2021500914A5 (enExample) | 2021-02-25 |
| JP6961819B2 JP6961819B2 (ja) | 2021-11-05 |
Family
ID=66331288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020524437A Active JP6961819B2 (ja) | 2017-11-01 | 2018-05-22 | L−リジンを生産する組換え菌、その構築方法およびl−リジンの生産方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20200347419A1 (enExample) |
| EP (1) | EP3690027A4 (enExample) |
| JP (1) | JP6961819B2 (enExample) |
| CN (1) | CN109750069B (enExample) |
| CA (1) | CA3081113A1 (enExample) |
| PH (1) | PH12020550529A1 (enExample) |
| WO (1) | WO2019085445A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110256535B (zh) * | 2019-06-14 | 2021-01-01 | 中国石油大学(华东) | L-天门冬酰胺酶XiDL及其编码基因和应用 |
| EP3986910A1 (en) * | 2019-06-24 | 2022-04-27 | Danisco US Inc. | Disruption of cdc42 effectors in yeast for increased alcohol and lysine production |
| CN110592109B (zh) * | 2019-08-28 | 2020-10-09 | 黑龙江伊品生物科技有限公司 | 一种spoT基因改造的重组菌株及其构建方法与应用 |
| CN112877269B (zh) * | 2020-01-15 | 2021-12-24 | 中国科学院天津工业生物技术研究所 | 生产赖氨酸的微生物以及赖氨酸的生产方法 |
| CN111607608B (zh) * | 2020-04-20 | 2023-04-14 | 天津科技大学 | 基因工程高产菌株淀粉酶产色链霉菌及提高ε-聚赖氨酸产量的方法和应用 |
| CN111850010B (zh) * | 2020-06-08 | 2021-04-09 | 黑龙江伊品生物科技有限公司 | 一种dapB基因改造的重组菌株及其构建方法与应用 |
| CN111979165B (zh) * | 2020-08-07 | 2021-05-07 | 黑龙江伊品生物科技有限公司 | 一种产l-赖氨酸的重组菌株及其构建方法与应用 |
| CN113755492B (zh) * | 2020-07-20 | 2023-05-30 | 中国科学院天津工业生物技术研究所 | 丙酮酸羧化酶基因启动子的突变体及其应用 |
| CN112301068B (zh) * | 2020-10-29 | 2022-10-04 | 齐齐哈尔龙江阜丰生物科技有限公司 | 一种葡萄糖母液水解综合利用工艺 |
| MX2023010588A (es) * | 2021-03-09 | 2023-12-08 | Cj Cheiljedang Corp | Variante de corynebacterium glutamicum que tiene capacidad de producción mejorada de l-lisina, y procedimiento para la producción de l-lisina mediante el uso de la misma. |
| CN112695036B (zh) * | 2021-03-23 | 2021-07-06 | 中国科学院天津工业生物技术研究所 | 一种天冬氨酸激酶基因表达调控序列及其应用 |
| MX2023012821A (es) * | 2021-04-29 | 2024-01-25 | Cj Cheiljedang Corp | Variante de corynebacterium glutamicum que tiene capacidad mejorada de producción de l-lisina, y procedimiento para producir l-lisina utilizando la misma. |
| MX2023012825A (es) * | 2021-04-30 | 2024-01-25 | Cj Cheiljedang Corp | Variante de corynebacterium glutamicum con capacidad mejorada de producción de l-lisina y procedimiento de producción de l-lisina mediante el uso de la misma. |
| CN113278571A (zh) * | 2021-05-27 | 2021-08-20 | 齐鲁工业大学 | 一种棒状杆菌工程菌构建方法及应用 |
| CN115449519B (zh) * | 2021-06-08 | 2023-04-07 | 中国科学院天津工业生物技术研究所 | 基于dapB基因的具有启动子活性的多核苷酸及其用途 |
| CN116179586A (zh) * | 2021-11-26 | 2023-05-30 | 上海凯赛生物技术股份有限公司 | 用于发酵生产l-赖氨酸的表达盒、菌株及其应用 |
| CN114908027B (zh) * | 2022-01-20 | 2023-12-26 | 天津科技大学 | 泛酸生产相关菌株及其构建方法与应用 |
| CN116004495B (zh) * | 2022-10-12 | 2025-12-16 | 浙江工业大学 | 生产β-丙氨酸的重组基因工程菌及其应用 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA01013123A (es) * | 1999-06-25 | 2002-06-21 | Basf Ag | Genes de corynebacterium glutamicum que codifican proteinas de via metabolica. |
| JP4623825B2 (ja) * | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
| DE102004035065A1 (de) * | 2004-07-20 | 2006-02-16 | Basf Ag | P-ET-TS-Expressionseinheiten |
| WO2010144746A2 (en) * | 2009-06-10 | 2010-12-16 | Genomatica, Inc. | Microorganisms and methods for carbon-efficient biosynthesis of mek and 2-butanol |
| KR101512432B1 (ko) * | 2010-06-15 | 2015-04-16 | 백광산업 주식회사 | 미생물을 이용한 아스파테이트 계열 아미노산의 생산방법 |
| AU2011278657B2 (en) * | 2010-07-14 | 2015-05-14 | Nestec S.A. | Asparaginase from Basidiomycetes |
| CN105734004B (zh) * | 2016-03-02 | 2020-06-19 | 廊坊梅花生物技术开发有限公司 | 重组菌株及其制备方法、应用 |
-
2017
- 2017-11-01 CN CN201711058221.4A patent/CN109750069B/zh active Active
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2018
- 2018-05-22 WO PCT/CN2018/087894 patent/WO2019085445A1/zh not_active Ceased
- 2018-05-22 CA CA3081113A patent/CA3081113A1/en active Pending
- 2018-05-22 US US16/760,662 patent/US20200347419A1/en not_active Abandoned
- 2018-05-22 EP EP18874429.6A patent/EP3690027A4/en active Pending
- 2018-05-22 JP JP2020524437A patent/JP6961819B2/ja active Active
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2020
- 2020-04-30 PH PH12020550529A patent/PH12020550529A1/en unknown
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