CA3064569C - Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing l-branched-chain amino acid using the same - Google Patents
Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing l-branched-chain amino acid using the same Download PDFInfo
- Publication number
- CA3064569C CA3064569C CA3064569A CA3064569A CA3064569C CA 3064569 C CA3064569 C CA 3064569C CA 3064569 A CA3064569 A CA 3064569A CA 3064569 A CA3064569 A CA 3064569A CA 3064569 C CA3064569 C CA 3064569C
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- Prior art keywords
- amino acid
- seq
- branched
- acid synthase
- acetohydroxy
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01006—Acetolactate synthase (2.2.1.6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/03—Oxo-acid-lyases (4.1.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2017-0087978 | 2017-07-11 | ||
| KR1020170087978A KR101996129B1 (ko) | 2017-07-11 | 2017-07-11 | 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법 |
| PCT/KR2018/007821 WO2019013532A2 (ko) | 2017-07-11 | 2018-07-10 | 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3064569A1 CA3064569A1 (en) | 2019-01-17 |
| CA3064569C true CA3064569C (en) | 2023-03-14 |
Family
ID=65002267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3064569A Active CA3064569C (en) | 2017-07-11 | 2018-07-10 | Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing l-branched-chain amino acid using the same |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US10844359B2 (https=) |
| EP (1) | EP3553171B1 (https=) |
| JP (1) | JP6794555B2 (https=) |
| KR (1) | KR101996129B1 (https=) |
| CN (2) | CN110724679B (https=) |
| AU (1) | AU2018301879B2 (https=) |
| BR (1) | BR112019020183B1 (https=) |
| CA (1) | CA3064569C (https=) |
| ES (1) | ES2919345T3 (https=) |
| MX (1) | MX2019015056A (https=) |
| RU (1) | RU2743964C1 (https=) |
| WO (1) | WO2019013532A2 (https=) |
| ZA (1) | ZA201908353B (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102147381B1 (ko) * | 2019-11-22 | 2020-08-24 | 씨제이제일제당 주식회사 | 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물 |
| KR102311391B1 (ko) * | 2020-05-21 | 2021-10-12 | 씨제이제일제당 주식회사 | L- 분지쇄 아미노산 생산능이 강화된 미생물 및 이를 이용하여 l-분지쇄 아미노산을 생산하는 방법 |
| CN112195204B (zh) * | 2020-10-21 | 2022-04-15 | 通辽梅花生物科技有限公司 | 一种混菌发酵生产支链氨基酸的方法 |
| KR102619598B1 (ko) * | 2021-08-23 | 2023-12-29 | 씨제이제일제당 주식회사 | 신규한 아세토하이드록시산 신타아제 소단위체 변이체 및 이를 이용한 l-발린 생산 방법 |
| KR102935702B1 (ko) * | 2022-12-28 | 2026-03-09 | 씨제이제일제당 주식회사 | 신규한 아세토락테이트 합성효소 활성을 갖는 변이체 폴리펩티드 및 이를 이용한 l-글루탐산 생산 방법 |
| CN121532502A (zh) * | 2023-05-19 | 2026-02-13 | Cj第一制糖株式会社 | 变体多肽和使用其生产l-缬氨酸的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6576455B1 (en) * | 1995-04-20 | 2003-06-10 | Basf Corporation | Structure-based designed herbicide resistant products |
| CA2380613C (en) * | 1999-08-02 | 2014-03-25 | Archer-Daniels-Midland Company | Production of l-lysine by corynebacterium strain having mutation in ilvbn operon |
| JP4623825B2 (ja) | 1999-12-16 | 2011-02-02 | 協和発酵バイオ株式会社 | 新規ポリヌクレオチド |
| EP1491634A1 (en) | 2003-06-26 | 2004-12-29 | Degussa AG | Feedback resistant acetohydroxy acid synthetase mutants |
| KR100671167B1 (ko) | 2005-09-12 | 2007-01-19 | 이상익 | 개인용 긴급 구난 벨트 |
| WO2007149069A2 (en) | 2006-06-15 | 2007-12-27 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Resistance to acetolactate synthase-inhibiting herbicides |
| RU2355763C2 (ru) * | 2006-09-13 | 2009-05-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Мутантная ацетолактатсинтаза и способ продукции разветвленных l-аминокислот |
| WO2008033001A1 (en) | 2006-09-15 | 2008-03-20 | Cj Cheiljedang Corporation | A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same |
| CN108130336A (zh) | 2007-10-05 | 2018-06-08 | 赛布斯欧洲公司 | 芸苔属中突变的乙酰羟酸合酶基因 |
| US9169502B2 (en) * | 2010-06-15 | 2015-10-27 | Paik Kwang Industrial Co., Ltd. | Method of producing L-lysine using a Corynebacterium glutamicum microorganism |
| CN102286505B (zh) | 2011-05-26 | 2013-04-03 | 江南大学 | 用于发酵生产l-缬氨酸的重组dna、菌株及方法 |
| KR101117022B1 (ko) | 2011-08-16 | 2012-03-16 | 씨제이제일제당 (주) | Ｌ-발린 생산능이 향상된 미생물 및 이를 이용한 ｌ-발린 제조방법 |
| EP2749638A4 (en) | 2011-08-22 | 2015-04-22 | Res Inst Innovative Tech Earth | MEANS FOR TRANSFORMING CORYNEFORMER BACTERIA AND PROCESS FOR VALIN PRODUCTION THEREWITH |
| KR101721722B1 (ko) * | 2013-03-11 | 2017-04-10 | 씨제이제일제당 (주) | L-발린 생산능이 향상된 균주 및 이를 이용한 l-발린 생산방법 |
| ES2623161T3 (es) | 2013-06-03 | 2017-07-10 | Evonik Degussa Gmbh | Procedimiento para la preparación de L-valina utilizando corinebacterias recombinantes que contienen el operón ilvBN inducible por propionato |
| KR20150119785A (ko) | 2014-04-15 | 2015-10-26 | 삼성전자주식회사 | 라이프 로그 서비스 제공 시스템 및 그 서비스 방법 |
| KR101720836B1 (ko) * | 2014-08-05 | 2017-04-03 | 씨제이제일제당 (주) | 피드백 저항성 아세토하이드록시산 신타아제 변이체 및 이를 이용한 l-발린의 생산방법 |
| KR101796830B1 (ko) | 2015-08-25 | 2017-11-13 | 씨제이제일제당 (주) | Ｌ-류신 생산능을 가지는 미생물 및 이를 이용한 ｌ-류신의 제조방법 |
| CN106754807B (zh) * | 2016-12-29 | 2020-10-30 | 廊坊梅花生物技术开发有限公司 | 生产l-亮氨酸菌株和生产l-亮氨酸的方法 |
-
2017
- 2017-07-11 KR KR1020170087978A patent/KR101996129B1/ko active Active
-
2018
- 2018-07-10 EP EP18832634.2A patent/EP3553171B1/en active Active
- 2018-07-10 MX MX2019015056A patent/MX2019015056A/es unknown
- 2018-07-10 WO PCT/KR2018/007821 patent/WO2019013532A2/ko not_active Ceased
- 2018-07-10 AU AU2018301879A patent/AU2018301879B2/en active Active
- 2018-07-10 CA CA3064569A patent/CA3064569C/en active Active
- 2018-07-10 BR BR112019020183-1A patent/BR112019020183B1/pt active IP Right Grant
- 2018-07-10 ES ES18832634T patent/ES2919345T3/es active Active
- 2018-07-10 CN CN201911130496.3A patent/CN110724679B/zh active Active
- 2018-07-10 JP JP2019538131A patent/JP6794555B2/ja active Active
- 2018-07-10 CN CN201880015829.5A patent/CN110506112B/zh active Active
- 2018-07-10 US US16/479,813 patent/US10844359B2/en active Active
- 2018-07-10 RU RU2019138301A patent/RU2743964C1/ru active
-
2019
- 2019-12-13 ZA ZA2019/08353A patent/ZA201908353B/en unknown
-
2020
- 2020-10-21 US US17/076,057 patent/US11085029B2/en active Active
-
2021
- 2021-06-24 US US17/357,196 patent/US11345901B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110506112B (zh) | 2021-06-08 |
| AU2018301879B2 (en) | 2022-02-17 |
| WO2019013532A3 (ko) | 2019-04-04 |
| CN110506112A (zh) | 2019-11-26 |
| AU2018301879A1 (en) | 2020-01-16 |
| JP6794555B2 (ja) | 2020-12-02 |
| MX2019015056A (es) | 2020-08-03 |
| US10844359B2 (en) | 2020-11-24 |
| EP3553171A2 (en) | 2019-10-16 |
| US20200263149A1 (en) | 2020-08-20 |
| KR101996129B1 (ko) | 2019-07-04 |
| ES2919345T3 (es) | 2022-07-26 |
| US11085029B2 (en) | 2021-08-10 |
| ZA201908353B (en) | 2021-04-28 |
| US11345901B2 (en) | 2022-05-31 |
| EP3553171B1 (en) | 2022-05-04 |
| BR112019020183A2 (pt) | 2020-06-02 |
| BR112019020183B1 (pt) | 2023-03-21 |
| RU2743964C1 (ru) | 2021-03-01 |
| KR20190007142A (ko) | 2019-01-22 |
| JP2020505023A (ja) | 2020-02-20 |
| US20210324349A1 (en) | 2021-10-21 |
| EP3553171A4 (en) | 2020-05-13 |
| WO2019013532A2 (ko) | 2019-01-17 |
| CN110724679A (zh) | 2020-01-24 |
| CN110724679B (zh) | 2022-01-11 |
| CA3064569A1 (en) | 2019-01-17 |
| US20210040457A1 (en) | 2021-02-11 |
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