CA2998642C - Selection of pluripotent cells for production of fertile xy female mice - Google Patents

Selection of pluripotent cells for production of fertile xy female mice Download PDF

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Publication number
CA2998642C
CA2998642C CA2998642A CA2998642A CA2998642C CA 2998642 C CA2998642 C CA 2998642C CA 2998642 A CA2998642 A CA 2998642A CA 2998642 A CA2998642 A CA 2998642A CA 2998642 C CA2998642 C CA 2998642C
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cell
cells
human mammalian
medium
uty
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CA2998642A
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English (en)
French (fr)
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CA2998642A1 (en
Inventor
Jennifer SCHMAHL
David Frendewey
Junko Kuno
Chia-Jen Siao
Gustavo Droguett
Yu Bai
Wojtek Auerbach
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/35—Animals modified by environmental factors, e.g. temperature, O2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2998642A 2015-09-17 2016-09-16 Selection of pluripotent cells for production of fertile xy female mice Active CA2998642C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562219927P 2015-09-17 2015-09-17
US62/219,927 2015-09-17
PCT/US2016/052345 WO2017049240A1 (en) 2015-09-17 2016-09-16 Selection of pluripotent cells for production of fertile xy female mice

Publications (2)

Publication Number Publication Date
CA2998642A1 CA2998642A1 (en) 2017-03-23
CA2998642C true CA2998642C (en) 2023-09-12

Family

ID=57113699

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2998642A Active CA2998642C (en) 2015-09-17 2016-09-16 Selection of pluripotent cells for production of fertile xy female mice

Country Status (13)

Country Link
US (2) US10893666B2 (enExample)
EP (1) EP3349573B1 (enExample)
JP (1) JP6868615B2 (enExample)
KR (1) KR102382458B1 (enExample)
CN (1) CN108697068B (enExample)
AU (1) AU2016324305B2 (enExample)
CA (1) CA2998642C (enExample)
ES (1) ES3067490T3 (enExample)
IL (1) IL257982B2 (enExample)
MX (1) MX388475B (enExample)
RU (1) RU2729407C1 (enExample)
SG (1) SG10201913789VA (enExample)
WO (1) WO2017049240A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102382458B1 (ko) 2015-09-17 2022-04-04 리제너론 파마슈티칼스 인코포레이티드 생식력 있는 xy 암컷 마우스의 생산을 위한 다능성 세포의 선택
CN108998540A (zh) * 2018-08-18 2018-12-14 兰州大学 绵羊eif2s3y基因的单核苷酸多态性标记及其检测引物、试剂盒、方法及应用
CN110656167A (zh) * 2019-09-27 2020-01-07 中国科学院苏州生物医学工程技术研究所 用于y染色体微缺失检测的引物组、试剂盒及基于数字pcr的检测方法
CN111321232B (zh) * 2020-03-18 2022-08-16 西北农林科技大学 一种快速检测肉牛eif4a2基因拷贝数变异的方法及其应用
CN113558012A (zh) * 2021-08-05 2021-10-29 陈米米 一种哺乳动物繁育雌性多胎的方法
CN114015705A (zh) * 2021-11-28 2022-02-08 华中科技大学同济医学院附属协和医院 一种小鼠体外受精繁育性别选择方法
CN115251007B (zh) * 2022-09-02 2024-01-26 山西医科大学 一种新品系裸大鼠、其培育方法及应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1217746A (zh) 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
EP1067141A1 (en) * 1999-06-11 2001-01-10 Rijksuniversiteit te Leiden A male-specific protein homologue involved in histocompatibility
CA2388510A1 (en) 1999-11-02 2001-05-10 James M. Robl Use of haploid genomes for genetic diagnosis, modification and multiplication
MXPA02006094A (es) 1999-12-20 2004-08-23 Anthony C F Perry Metodos para producir embriones clonadod y adultos a partir de celulas cultivadas.
CA2477112A1 (en) 2002-02-27 2003-09-04 Yasumitsu Nagao A method for producing germline chimeric animal
ATE306810T1 (de) 2002-03-05 2005-11-15 Artemis Pharmaceuticals Gmbh Durch inzucht erzeugte von embryonalen stammzellen abgeleitete mäuse
EP1516924A1 (en) 2003-09-17 2005-03-23 Fundacion IVI para el Estudio de la reproduccion Humana (FIVIER) Generation of human embryonic stem cells from triploid zygotes
SG150567A1 (en) 2004-03-23 2009-03-30 Akaike Toshihiro Pluripotent stem cell growing method
EP1802193B1 (en) 2004-10-19 2014-04-30 Regeneron Pharmaceuticals, Inc. Method for generating a mouse homozygous for a genetic modification
WO2008017704A1 (en) 2006-08-09 2008-02-14 Vivalis Method of production of transgenic avian using embryonic stem cells
GB0623635D0 (en) 2006-11-27 2007-01-03 Stem Cell Sciences Uk Ltd Pluripotent cell growth media
WO2009037337A1 (en) 2007-09-20 2009-03-26 Novartis Ag Robust and tissue independent gender-specific transcript markers for molecular gender determination
US20120084873A1 (en) 2009-02-08 2012-04-05 Andrew Sinclair Sex-determination and methods of specifying same
AU2010306377B2 (en) 2009-10-13 2015-01-22 Stemcell Technologies Canada Inc. Manipulation of osmolality for differentiating stem cells
JP2013528062A (ja) 2010-06-11 2013-07-08 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Ｘｙｅｓ細胞からの繁殖可能なｘｙ雌動物の生成
WO2012022634A1 (en) * 2010-08-16 2012-02-23 Institut National De La Sante Et De La Recherche Medicale (Inserm) Classification, diagnosis and prognosis of multiple myeloma
DK3161128T3 (en) 2014-06-26 2018-11-05 Regeneron Pharma METHODS AND COMPOSITIONS FOR TARGETED GENTICAL MODIFICATIONS AND PROCEDURES FOR USE THEREOF
BR112017007770A2 (pt) 2014-10-15 2018-01-16 Regeneron Pharma cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
KR102382458B1 (ko) 2015-09-17 2022-04-04 리제너론 파마슈티칼스 인코포레이티드 생식력 있는 xy 암컷 마우스의 생산을 위한 다능성 세포의 선택

Also Published As

Publication number Publication date
EP3349573C0 (en) 2026-02-18
CN108697068A (zh) 2018-10-23
US20210112788A1 (en) 2021-04-22
IL257982A (en) 2018-05-31
IL257982B1 (en) 2023-01-01
AU2016324305B2 (en) 2022-03-03
EP3349573A1 (en) 2018-07-25
CN108697068B (zh) 2022-02-11
RU2729407C1 (ru) 2020-08-06
ES3067490T3 (en) 2026-05-22
NZ740899A (en) 2021-11-26
IL257982B2 (en) 2023-05-01
AU2016324305A1 (en) 2018-04-12
US10893666B2 (en) 2021-01-19
CA2998642A1 (en) 2017-03-23
WO2017049240A1 (en) 2017-03-23
KR102382458B1 (ko) 2022-04-04
JP2018527005A (ja) 2018-09-20
SG10201913789VA (en) 2020-03-30
WO2017049240A8 (en) 2018-05-24
MX388475B (es) 2025-03-20
KR20180058731A (ko) 2018-06-01
US12041918B2 (en) 2024-07-23
JP6868615B2 (ja) 2021-05-12
US20170079250A1 (en) 2017-03-23
EP3349573B1 (en) 2026-02-18
HK1256687A1 (en) 2019-10-04
AU2016324305A9 (en) 2019-01-03
MX2018003354A (es) 2018-05-28

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