CA2994166C - Engineered crispr-cas9 compositions and methods of use - Google Patents

Engineered crispr-cas9 compositions and methods of use Download PDF

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Publication number
CA2994166C
CA2994166C CA2994166A CA2994166A CA2994166C CA 2994166 C CA2994166 C CA 2994166C CA 2994166 A CA2994166 A CA 2994166A CA 2994166 A CA2994166 A CA 2994166A CA 2994166 C CA2994166 C CA 2994166C
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nucleotide sequence
polynucleotide
element nucleotide
stem element
nexus
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CA2994166A1 (en
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Paul Daniel Donohoue
Andrew Paul May
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Caribou Biosciences Inc
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Caribou Biosciences Inc
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
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    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CA2994166A 2015-08-07 2016-08-05 Engineered crispr-cas9 compositions and methods of use Active CA2994166C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562202715P 2015-08-07 2015-08-07
US62/202,715 2015-08-07
US201562209334P 2015-08-24 2015-08-24
US62/209,334 2015-08-24
PCT/US2016/045915 WO2017027423A1 (en) 2015-08-07 2016-08-05 Engineered crispr-cas9 compositions and methods of use

Publications (2)

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CA2994166A1 CA2994166A1 (en) 2017-02-16
CA2994166C true CA2994166C (en) 2019-06-18

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US (5) US9580727B1 (https=)
EP (2) EP3461894B1 (https=)
JP (1) JP6649468B2 (https=)
KR (1) KR102012382B1 (https=)
CN (1) CN107922944B (https=)
AU (1) AU2016304795B2 (https=)
CA (1) CA2994166C (https=)
DK (1) DK3320092T3 (https=)
ES (1) ES2705540T3 (https=)
GB (1) GB2556276A (https=)
HK (1) HK1247238B (https=)
IL (1) IL257307B (https=)
MX (1) MX2018001617A (https=)
NZ (1) NZ738689A (https=)
WO (1) WO2017027423A1 (https=)
ZA (1) ZA201800330B (https=)

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