CA2981622C - High resolution systems, kits, apparatus, and methods for high throughput microbiology applications - Google Patents

High resolution systems, kits, apparatus, and methods for high throughput microbiology applications Download PDF

Info

Publication number
CA2981622C
CA2981622C CA2981622A CA2981622A CA2981622C CA 2981622 C CA2981622 C CA 2981622C CA 2981622 A CA2981622 A CA 2981622A CA 2981622 A CA2981622 A CA 2981622A CA 2981622 C CA2981622 C CA 2981622C
Authority
CA
Canada
Prior art keywords
microwells
cells
microwell
cell
high density
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2981622A
Other languages
English (en)
French (fr)
Other versions
CA2981622A1 (en
Inventor
Paul C. Blainey
Michael W. SEELY
Roman STOCKER
Karsten Zengler
Scott Conradson
Peter CHRISTEY
Alexander Hallock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Isolation Bio Inc
Original Assignee
Isolation Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isolation Bio Inc filed Critical Isolation Bio Inc
Priority to CA3184363A priority Critical patent/CA3184363C/en
Publication of CA2981622A1 publication Critical patent/CA2981622A1/en
Application granted granted Critical
Publication of CA2981622C publication Critical patent/CA2981622C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/021—Identification, e.g. bar codes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0244—Drop counters; Drop formers using pins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
CA2981622A 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications Active CA2981622C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3184363A CA3184363C (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201562150677P 2015-04-21 2015-04-21
US62/150,677 2015-04-21
US201662292091P 2016-02-05 2016-02-05
US62/292,091 2016-02-05
US201662299088P 2016-02-24 2016-02-24
US62/299,088 2016-02-24
PCT/US2016/028681 WO2016172362A1 (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3184363A Division CA3184363C (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications

Publications (2)

Publication Number Publication Date
CA2981622A1 CA2981622A1 (en) 2016-10-27
CA2981622C true CA2981622C (en) 2023-02-07

Family

ID=55863246

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2981622A Active CA2981622C (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
CA3184363A Active CA3184363C (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3184363A Active CA3184363C (en) 2015-04-21 2016-04-21 High resolution systems, kits, apparatus, and methods for high throughput microbiology applications

Country Status (10)

Country Link
US (5) US10900073B2 (enExample)
EP (2) EP4070887A1 (enExample)
JP (2) JP6837473B2 (enExample)
CN (2) CN114015569A (enExample)
AU (2) AU2016250591B2 (enExample)
CA (2) CA2981622C (enExample)
DK (1) DK3285926T3 (enExample)
IL (2) IL254858B (enExample)
SG (1) SG11201708579WA (enExample)
WO (1) WO2016172362A1 (enExample)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
WO2015175398A1 (en) 2014-05-12 2015-11-19 President And Fellows Of Harvard College Systems and methods for making and using sensors, probes, and other devices
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
US10865434B2 (en) 2015-04-21 2020-12-15 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
US10788452B2 (en) 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
US10712356B2 (en) * 2015-04-21 2020-07-14 General Automation Lab Technologies Inc. Apparatus and method for picking biological sample
CN114015569A (zh) * 2015-04-21 2022-02-08 通用自动化实验技术公司 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
US9938558B2 (en) 2015-06-25 2018-04-10 Ascus Biosciences, Inc. Methods, apparatuses, and systems for analyzing microorganism strains from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon
TW201713775A (zh) 2015-06-25 2017-04-16 亞斯科生物科學公司 用於自複雜的異源群集中分析微生物品系、預測及識別其機能性關係及交互作用，且依據前述選擇並合成微生物系集之方法、裝置及系統
US10851399B2 (en) 2015-06-25 2020-12-01 Native Microbials, Inc. Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon
EP3400285B1 (en) 2016-01-07 2025-10-29 Native Microbials, Inc. Methods for improving milk production by administration of microbial consortia
US10672601B2 (en) * 2016-06-07 2020-06-02 The Regents Of The University Of California Detecting compounds in microfluidic droplets using mass spectrometry
EP3478650B1 (en) * 2016-06-30 2021-08-04 General Automation Lab Technologies Inc. Methods using lateral flow for high throughput microbiology applications
WO2018017106A1 (en) 2016-07-21 2018-01-25 Rutgers, The State University Of New Jersey Endophytic bacterium for application to grasses to increase plant growth
WO2018027185A1 (en) 2016-08-04 2018-02-08 Duke University Compositions and methods for measuring bacterial growth
EP3519096A4 (en) * 2016-09-28 2020-06-17 General Automation Lab Technologies, Inc. HIGH RESOLUTION SYSTEMS, KITS, APPARATUS AND METHODS FOR DETERMINING BACTERIAL COMMUNITY RELATIONSHIP AND OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS
CN110168069A (zh) * 2016-10-19 2019-08-23 通用自动化实验技术公司 用于筛选微生物和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
CA3048248A1 (en) 2016-12-28 2018-07-05 Ascus Biosciences, Inc. Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities with tracer analytics, determination of functional relationships and interactions thereof, and synthesis of microbial ensembles including dosed microbial ensembles and inoculative microbial ensembles
AU2017386658A1 (en) 2016-12-28 2019-07-25 Native Microbials, Inc. Methods, apparatuses, and systems for analyzing complete microorganism strains in complex heterogeneous communities, determining functional relationships and interactions thereof, and identifying and synthesizing bioreactive modificators based thereon
CN110769925A (zh) * 2017-03-29 2020-02-07 康奈尔大学 使用组合的核酸酶、连结酶、聚合酶以及测序反应测定核酸序列、表达、拷贝数或甲基化变化的装置、方法以及系统
CA3057965A1 (en) * 2017-04-12 2018-10-18 General Automation Lab Technologies Inc. Apparatus and method for picking biological sample
EP3614847A4 (en) 2017-04-28 2021-09-01 Native Microbials, Inc. PROCESSES PROMOTING ENERGY AND / OR CEREAL RICH DIETS BY MEANS OF A SYNTHETIC BIO-SET OF MICROBES
CN107287293B (zh) * 2017-06-13 2019-07-30 浙江大学 一种环境中微生物群落结构的绝对丰度测定方法
EP3848459A1 (en) 2017-06-30 2021-07-14 Inscripta, Inc. Automated cell processing methods, modules, instruments and systems
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
EP3679354B1 (en) 2017-09-08 2024-07-24 President And Fellows Of Harvard College Nanosensor methods and apparatuses for determination of analytes
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
EP3775159A4 (en) 2018-03-29 2022-01-19 Inscripta, Inc. AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
WO2020005383A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
CN113543882A (zh) * 2018-12-31 2021-10-22 本-古里安大学B.G.内盖夫技术和应用公司 捕获流测定装置和方法
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
KR20210134692A (ko) 2019-02-27 2021-11-10 나노모자이크 인크. 나노센서들 및 그것의 사용
EP3962653A4 (en) * 2019-05-01 2023-09-13 Massachusetts Institute Of Technology MASSIVELY PARALLEL ON-CHIP CONSTRUCTION OF SYNTHETIC MICROBIAN COMMUNITIES
WO2020223697A1 (en) * 2019-05-02 2020-11-05 General Automation Lab Technologies Inc. Microfabricated device with hydrophilic microwells and hydrophobic interstitial space
WO2020232425A1 (en) * 2019-05-16 2020-11-19 General Automation Lab Technologies Inc. Method for assaying biological sample on microfabricated chip
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US11427865B2 (en) * 2019-07-12 2022-08-30 California Institute Of Technology Absolute quantification of nucleic acids and related methods and systems
US12173361B2 (en) * 2019-07-12 2024-12-24 California Institute Of Technology Rodent model and related device, compositions, methods and systems
EP4045897A4 (en) * 2019-10-18 2023-12-20 Isolation Bio Inc. USE OF RESORUFIN TO MONITOR THE METABOLIC ACTIVITY OF CELLS UNDER ANAEROBIC CONDITIONS
US12157124B2 (en) 2019-11-06 2024-12-03 10X Genomics, Inc. Imaging system hardware
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
CN115135370A (zh) 2020-01-27 2022-09-30 因思科瑞普特公司 电穿孔模块和仪器
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11684914B2 (en) 2020-02-18 2023-06-27 Isolation Bio Inc. Method of transferring material from microfabricated device
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
ES2999535T3 (en) 2020-06-10 2025-02-26 10X Genomics Inc Methods for determining a location of an analyte in a biological sample
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
ES2993269T3 (en) 2020-09-18 2024-12-26 10X Genomics Inc Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
CN112553064A (zh) * 2020-12-24 2021-03-26 广东省微生物研究所（广东省微生物分析检测中心） 一种高通量筛选具有潜在生物防腐功能活性菌株的装置及方法
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
CN113083383B (zh) * 2021-03-18 2022-10-25 华中农业大学 微流控芯片装置、制备方法及土壤微生物群落培养方法
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
USD1064308S1 (en) 2021-09-17 2025-02-25 10X Genomics, Inc. Sample handling device
WO2023065174A1 (zh) * 2021-10-20 2023-04-27 深圳华大生命科学研究院 筛选药物的方法
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US20240141278A1 (en) 2022-11-02 2024-05-02 Fujifilm Corporation Pattern forming device and pattern forming method

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE8137962U1 (de) * 1981-12-28 1982-06-16 Biotest-Serum-Institut Gmbh, 6000 Frankfurt Mikrotiterplatte zur blutgruppendiagnostik
US4634676A (en) 1984-06-06 1987-01-06 Becton, Dickinson And Company Replica plating device
US4717667A (en) * 1984-07-11 1988-01-05 Fmc Corporation Colony replicating device
US5061621A (en) 1989-06-22 1991-10-29 Brandeis University Replica plating device with an integral marking element
AUPN154295A0 (en) 1995-03-06 1995-03-30 Commonwealth Scientific And Industrial Research Organisation Nucleic acid molecule encoding a protein with avian gamma-interferon activity
US5587322A (en) 1994-11-23 1996-12-24 Replicatech, Inc. Replica plating device
US5985594A (en) 1995-11-14 1999-11-16 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US6972183B1 (en) 1997-06-16 2005-12-06 Diversa Corporation Capillary array-based enzyme screening
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6103479A (en) 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US5882922A (en) 1997-03-19 1999-03-16 Becton Dickinson And Company Culture vessel assembly
US7019827B2 (en) 1997-06-16 2006-03-28 Diversa Corporation GigaMatrix holding tray having through-hole wells
US20050070005A1 (en) 1997-06-16 2005-03-31 Martin Keller High throughput or capillary-based screening for a bioactivity or biomolecule
US5858770A (en) 1997-09-30 1999-01-12 Brandeis University Cell culture plate with oxygen and carbon dioxide-permeable waterproof sealing membrane
EP1124592B1 (en) 1998-10-28 2006-06-07 Cellon S.A. Textured and porous silicone rubber
US6455310B1 (en) * 1999-03-23 2002-09-24 Biocrystal Ltd. Cell culture apparatus and method for culturing cells
CA2319205A1 (en) 2000-03-13 2001-09-13 The State Of Oregon Acting By And Through The State Board Of Higher Educ Ation On Behalf Of Oregon State University High-throughput microbial culturing
US6638730B2 (en) 2001-03-06 2003-10-28 Council Of Scientific & Industrial Research Composition for qualitative screening of phosphate solubilizing microorganisms and a qualitative method for screening microorganisms
US7011957B2 (en) 2001-09-26 2006-03-14 Northeastern University Isolation and cultivation of microorganisms from natural environments and drug discovery based thereon
WO2003046508A2 (en) 2001-11-09 2003-06-05 Biomicroarrays, Inc. High surface area substrates for microarrays and methods to make same
US20050147959A1 (en) * 2002-03-25 2005-07-07 Frondoza Carmelita G. Tissue analogs for in vitro testing and method of use therefor
EP1527339B1 (en) 2002-06-03 2006-08-16 PamGene B.V. Method for high throughput cell-based assays using versatile living microarrays
DK1539925T3 (da) * 2002-09-16 2008-02-18 Pan Biotech Gmbh Cellekulturkammer til et cellekultursystem
JP3723882B2 (ja) * 2002-11-14 2005-12-07 篤 村口 抗原特異的リンパ球検出用マイクロウェルアレイチップ、抗原特異的リンパ球の検出法及び製造方法
WO2005052582A2 (en) 2003-11-28 2005-06-09 Pamgene B.V. Methods and apparatus for cell based microarray assays
US7833709B2 (en) 2004-05-28 2010-11-16 Wafergen, Inc. Thermo-controllable chips for multiplex analyses
EP1877533A2 (en) 2005-04-18 2008-01-16 Dsm Ip Assets B.V. Biochip and process for the production of a biochip
US7745209B2 (en) 2005-07-26 2010-06-29 Corning Incorporated Multilayered cell culture apparatus
US7776553B2 (en) * 2005-09-16 2010-08-17 Presidents And Fellows Of Harvard College Screening assays and methods
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
DE102006030068A1 (de) * 2006-06-28 2008-01-03 M2P-Labs Gmbh Vorrichtung und Verfahren zur Zu- und Abfuhr von Fluiden in geschüttelten Mikroreaktoren Arrays
JP2010518855A (ja) 2007-02-22 2010-06-03 コーニング インコーポレイテッド 細胞培養に有用な基体およびそれを製造し、使用する方法
WO2009123756A1 (en) 2008-04-05 2009-10-08 Single Cell Technology, Inc. Multi-well system
CN102046773A (zh) * 2008-05-30 2011-05-04 康宁股份有限公司 具有不同微孔外形的细胞培养装置
WO2009145875A1 (en) 2008-05-30 2009-12-03 Corning Incorporated Method for ultrasonic cell removal
US20110237445A1 (en) * 2008-10-10 2011-09-29 Picovitro Ab Genetic analysis in microwells
WO2010093766A1 (en) 2009-02-11 2010-08-19 The University Of North Carolina At Chapel Hill Method and device for cell selection and collection in an isolated culturing environment
WO2011044437A2 (en) 2009-10-09 2011-04-14 Stc.Unm Polony sequencing methods
US20130004967A1 (en) * 2009-11-23 2013-01-03 Halverson Kurt J Microwell array articles and methods of use
US9234229B2 (en) 2009-12-08 2016-01-12 The Board Of Trustees Of The Leland Stanford Junior University High throughput system for isolation, growth, and detection of lipid inclusions in bacteria
EP2536819B1 (en) 2010-02-16 2024-12-18 The University of North Carolina at Chapel Hill Array of micromolded structures for collecting or culturing of cells
US20130115606A1 (en) * 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
EP2407242A1 (en) * 2010-07-13 2012-01-18 Dublin City University Direct clone analysis and selection technology
KR102482184B1 (ko) 2010-12-22 2022-12-28 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
US9095852B2 (en) 2011-08-22 2015-08-04 The Regents Of The University Of California Multilayer high density microwells
CA2852631A1 (en) * 2011-10-28 2013-05-02 Itek Ventures Pty Ltd A gene and mutations thereof associated with seizure and movement disorders
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US20130295551A1 (en) 2012-03-29 2013-11-07 The Board Of Trustees Of The University Of Illinois Microfluidic device and method for modulating a gas environment of cell cultures and tissues
ES2899687T3 (es) 2012-05-14 2022-03-14 Irepertoire Inc Método para aumentar la precisión en la detección cuantitativa de polinucleótidos
DK2861760T3 (da) * 2012-06-15 2020-03-09 Univ Texas Sekventering med høj kapacitet af flere udskrifter af en enkelt celle
US9657290B2 (en) * 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
CN111748607B (zh) * 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
JP6197039B2 (ja) 2012-08-24 2017-09-13 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔｙ ハイスループット多重検出用システム、装置及び方法
EP2925782B1 (en) * 2012-12-03 2020-01-22 NovImmune SA Anti-cd47 antibodies and methods of use thereof
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
EP2805769A1 (en) * 2013-05-24 2014-11-26 European Molecular Biology Laboratory Methods for nano-scale single cell analysis
WO2014201273A1 (en) 2013-06-12 2014-12-18 The Broad Institute, Inc. High-throughput rna-seq
US20150017714A1 (en) 2013-07-02 2015-01-15 Miltenyi Biotec Gmbh Centrifugation chamber with gas permeable membrane layers for cell cultivation
ES2857908T3 (es) * 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
CA2868703A1 (en) 2013-10-25 2015-04-25 Ponnambalam Selvaganapathy Method and device for detecting metabollically active cells
NZ730044A (en) 2014-09-15 2024-08-30 Abvitro Llc High-throughput nucleotide library sequencing
WO2016064838A1 (en) 2014-10-20 2016-04-28 Northeastern University Device and method for high throughput bacterial isolation
US10370653B2 (en) 2015-02-22 2019-08-06 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
CN107548416B (zh) 2015-02-27 2021-07-13 康宁股份有限公司 用于多孔板的契合盖子
US10677793B2 (en) 2015-04-21 2020-06-09 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
US10865434B2 (en) * 2015-04-21 2020-12-15 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
US10712356B2 (en) * 2015-04-21 2020-07-14 General Automation Lab Technologies Inc. Apparatus and method for picking biological sample
US10788452B2 (en) * 2015-04-21 2020-09-29 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
CN114015569A (zh) * 2015-04-21 2022-02-08 通用自动化实验技术公司 用于高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
US20180023045A1 (en) * 2015-04-21 2018-01-25 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using combinatorial media strategies for high throughput microbiology applications
US10590461B2 (en) * 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
WO2017123722A1 (en) 2016-01-12 2017-07-20 Cleveland State University 3d-printed miniature biological constructs

Also Published As

Publication number Publication date
JP6837473B2 (ja) 2021-03-03
EP3285926A1 (en) 2018-02-28
CN114015569A (zh) 2022-02-08
IL254858B (en) 2021-12-01
AU2021257901A1 (en) 2021-11-18
CA2981622A1 (en) 2016-10-27
JP2018515133A (ja) 2018-06-14
DK3285926T3 (da) 2022-05-09
US20180051327A1 (en) 2018-02-22
CA3184363A1 (en) 2016-10-27
CN107810277B (zh) 2021-11-02
IL254858A0 (en) 2017-12-31
EP3285926B1 (en) 2022-03-02
AU2016250591A1 (en) 2017-10-26
US11104939B2 (en) 2021-08-31
CN107810277A (zh) 2018-03-16
IL287247A (en) 2021-12-01
US20190194734A1 (en) 2019-06-27
AU2016250591B2 (en) 2021-08-05
US20160312275A1 (en) 2016-10-27
SG11201708579WA (en) 2017-11-29
JP2020195385A (ja) 2020-12-10
US10227640B2 (en) 2019-03-12
IL287247B1 (en) 2023-03-01
US11136621B2 (en) 2021-10-05
US10900073B2 (en) 2021-01-26
WO2016172362A1 (en) 2016-10-27
US20190194735A1 (en) 2019-06-27
US11104940B2 (en) 2021-08-31
CA3184363C (en) 2024-01-02
EP4070887A1 (en) 2022-10-12
IL287247B2 (en) 2023-07-01
US20190194736A1 (en) 2019-06-27

Similar Documents

Publication Publication Date Title
US11136621B2 (en) High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
US12188075B2 (en) High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
US10677793B2 (en) High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
US20180023045A1 (en) High resolution systems, kits, apparatus, and methods using combinatorial media strategies for high throughput microbiology applications
WO2018075701A1 (en) High resolution systems, kits, apparatus, and methods for screening microorganisms and other high throughput microbiology applications
US10788452B2 (en) High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
CA3037631A1 (en) High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications
EP3478416A1 (en) High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
US20200263227A1 (en) High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
CA3027158A1 (en) High resolution systems, kits, apparatus, and methods using combinatorial media strategies for high throughput microbiology applications
EP3478650B1 (en) Methods using lateral flow for high throughput microbiology applications

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415

EEER Examination request

Effective date: 20210415