CA2924987C - Multiaptamer target detection - Google Patents
Multiaptamer target detection Download PDFInfo
- Publication number
- CA2924987C CA2924987C CA2924987A CA2924987A CA2924987C CA 2924987 C CA2924987 C CA 2924987C CA 2924987 A CA2924987 A CA 2924987A CA 2924987 A CA2924987 A CA 2924987A CA 2924987 C CA2924987 C CA 2924987C
- Authority
- CA
- Canada
- Prior art keywords
- aptamer
- deoxyuridine
- target
- complex
- aptamers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/205—Aptamer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361881629P | 2013-09-24 | 2013-09-24 | |
| US61/881,629 | 2013-09-24 | ||
| PCT/US2014/057143 WO2015048084A1 (en) | 2013-09-24 | 2014-09-24 | Multiaptamer target detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2924987A1 CA2924987A1 (en) | 2015-04-02 |
| CA2924987C true CA2924987C (en) | 2022-08-16 |
Family
ID=52744404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2924987A Active CA2924987C (en) | 2013-09-24 | 2014-09-24 | Multiaptamer target detection |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US9926566B2 (ko) |
| EP (1) | EP3049523B1 (ko) |
| JP (1) | JP6506768B2 (ko) |
| KR (1) | KR102348283B1 (ko) |
| CN (2) | CN105555957A (ko) |
| AU (1) | AU2014326975B2 (ko) |
| BR (1) | BR112016005957A2 (ko) |
| CA (1) | CA2924987C (ko) |
| HK (1) | HK1218765A1 (ko) |
| IL (1) | IL244284B (ko) |
| MX (1) | MX361451B (ko) |
| SG (1) | SG11201602063UA (ko) |
| WO (1) | WO2015048084A1 (ko) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3049523B1 (en) | 2013-09-24 | 2019-08-07 | Somalogic, Inc. | Multiaptamer target detection |
| EP3224361B1 (en) | 2014-11-24 | 2019-06-19 | Somalogic, Inc. | Nucleic acid compounds for binding growth differentiation factor 11 |
| EP3387152B1 (en) * | 2015-12-08 | 2022-01-26 | Twinstrand Biosciences, Inc. | Improved adapters, methods, and compositions for duplex sequencing |
| EP3430022B1 (en) | 2016-03-14 | 2020-03-11 | Somalogic, Inc. | Compounds and methods for the synthesis of 5-(n-protected-tryptaminocarboxyamide)-2'-deoxyuridine phosphoramidite for incorporation into a nucleic acid sequence |
| KR20190017870A (ko) * | 2016-06-17 | 2019-02-20 | 프록시머티 바이오사이언시스, 엘엘씨 | 앱타머 쌍 선택 방법 |
| EP3478843B1 (en) * | 2016-07-01 | 2021-08-04 | Somalogic, Inc. | Oligonucleotides comprising modified nucleosides |
| CN110249082B (zh) | 2016-12-01 | 2023-07-07 | 诺迪勒思附属公司 | 测定蛋白质的方法 |
| WO2019103131A1 (ja) * | 2017-11-24 | 2019-05-31 | 国立大学法人 東京大学 | アプタマーの結合部位判別方法、アプタマーペアおよび目的物質からなる三元錯体の解離定数等の測定方法並びに最適なアプタマーペアの選択方法 |
| CA3104041A1 (en) * | 2018-06-22 | 2019-12-26 | Somalogic, Inc. | Improved proteomic multiplex assays |
| EP4231174A3 (en) | 2020-06-11 | 2023-11-01 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| EP4281774A2 (en) | 2021-01-20 | 2023-11-29 | Nautilus Subsidiary, Inc. | Methods for biomolecule quantitation |
| CA3203535A1 (en) | 2021-01-21 | 2022-07-28 | Gregory KAPP | Systems and methods for biomolecule preparation |
| AU2022232933A1 (en) | 2021-03-11 | 2023-09-07 | Nautilus Subsidiary, Inc. | Systems and methods for biomolecule retention |
| WO2023038859A1 (en) | 2021-09-09 | 2023-03-16 | Nautilus Biotechnology, Inc. | Characterization and localization of protein modifications |
| CA3227592A1 (en) | 2021-09-22 | 2023-03-30 | Gregory KAPP | Methods and systems for determining polypeptide interactions |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| AU2023241893A1 (en) | 2022-03-29 | 2024-09-26 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| US20230360732A1 (en) | 2022-04-25 | 2023-11-09 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| WO2023250364A1 (en) | 2022-06-21 | 2023-12-28 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| US20240094215A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| WO2024107857A1 (en) | 2022-11-15 | 2024-05-23 | Nautilus Subsidiary, Inc. | Standard polypeptides |
| US20240192202A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | Fluidic media for single-analtye arrays |
| WO2024130000A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| WO2024151373A1 (en) | 2023-01-12 | 2024-07-18 | Nautilus Subsidiary, Inc. | Characterization of glycans and glycoconjugates |
| WO2024206122A1 (en) | 2023-03-24 | 2024-10-03 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2001257091A1 (en) * | 2000-04-18 | 2001-10-30 | Gilead Sciences, Inc. | Aptamer based two-site binding assay |
| US7312325B2 (en) * | 2000-09-26 | 2007-12-25 | Duke University | RNA aptamers and methods for identifying the same |
| US20070071744A1 (en) * | 2002-06-07 | 2007-03-29 | Gotz Munch | Agents which bind to epitopes of glycoprotein VI |
| US7595160B2 (en) | 2004-01-13 | 2009-09-29 | U.S. Genomics, Inc. | Analyte detection using barcoded polymers |
| US7795009B2 (en) * | 2005-06-15 | 2010-09-14 | Saint Louis University | Three-component biosensors for detecting macromolecules and other analytes |
| US20100035247A1 (en) | 2005-11-04 | 2010-02-11 | U.S. Genomics, Inc. | Heterogeneous Assay of Analytes in Solution Using Polymers |
| WO2008039642A2 (en) | 2006-09-26 | 2008-04-03 | Replidyne, Inc. | Methods and compounds for treatment of clostridium based infection |
| US7947447B2 (en) | 2007-01-16 | 2011-05-24 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
| JP5404620B2 (ja) * | 2007-07-17 | 2014-02-05 | ソマロジック・インコーポレーテッド | 試験試料の多重化分析 |
| WO2009037264A2 (en) | 2007-09-17 | 2009-03-26 | Da Volterra | Colonic delivery of antimicrobial agents |
| US8569252B2 (en) | 2009-04-15 | 2013-10-29 | Postech Academy-Industry Foundation | Nucleolin specific aptamer and use thereof |
| US20110256535A1 (en) | 2010-02-11 | 2011-10-20 | Intelligent Medical Devices, Inc. | Optimized oligonucleotides and methods of using same for the detection, isolation, amplification, quantification, monitoring, screening and sequencing of clostridium difficile genes encoding toxin b, and/or toxin a and/or binary toxin |
| GB201002627D0 (en) | 2010-02-16 | 2010-03-31 | Loxbridge Res Llp | Aptamer based analyte detection method |
| JP6012591B2 (ja) * | 2010-04-12 | 2016-10-26 | ソマロジック, インコーポレイテッドSomaLogic, Inc. | β−ＮＧＦに対するアプタマー及びβ−ＮＧＦ介在疾患及び障害の治療におけるその使用 |
| US8785132B2 (en) * | 2010-04-23 | 2014-07-22 | Postech Academy-Industry Foundation | Aptamer sandwich assays |
| US20150031585A1 (en) | 2011-03-10 | 2015-01-29 | Somalogic, Inc. | Multiaptamer Target Detection |
| KR20140015455A (ko) | 2011-03-10 | 2014-02-06 | 소마로직, 인크. | 클로스트리디움 디피실 진단을 위한 압타머 |
| CA2834200A1 (en) * | 2011-04-26 | 2012-11-01 | Regado Biosciences, Inc. | A method for manufacturing pegylated oligonucleotides |
| EP2761289B1 (en) | 2011-09-30 | 2020-02-12 | Somalogic, Inc. | Cardiovascular risk event prediction and uses thereof |
| WO2013102101A1 (en) | 2011-12-30 | 2013-07-04 | Somalogic, Inc. | Aptamers and diagnostic methods for detecting the egf receptor |
| EP3049523B1 (en) | 2013-09-24 | 2019-08-07 | Somalogic, Inc. | Multiaptamer target detection |
-
2014
- 2014-09-24 EP EP14847780.5A patent/EP3049523B1/en active Active
- 2014-09-24 SG SG11201602063UA patent/SG11201602063UA/en unknown
- 2014-09-24 CN CN201480051210.1A patent/CN105555957A/zh active Pending
- 2014-09-24 CN CN202210264586.7A patent/CN114703193A/zh active Pending
- 2014-09-24 JP JP2016544355A patent/JP6506768B2/ja active Active
- 2014-09-24 WO PCT/US2014/057143 patent/WO2015048084A1/en active Application Filing
- 2014-09-24 AU AU2014326975A patent/AU2014326975B2/en active Active
- 2014-09-24 CA CA2924987A patent/CA2924987C/en active Active
- 2014-09-24 US US15/023,182 patent/US9926566B2/en active Active
- 2014-09-24 MX MX2016003320A patent/MX361451B/es active IP Right Grant
- 2014-09-24 KR KR1020167006262A patent/KR102348283B1/ko active IP Right Grant
- 2014-09-24 BR BR112016005957A patent/BR112016005957A2/pt not_active IP Right Cessation
-
2016
- 2016-02-25 IL IL244284A patent/IL244284B/en active IP Right Grant
- 2016-06-13 HK HK16106780.3A patent/HK1218765A1/zh unknown
-
2018
- 2018-02-13 US US15/895,723 patent/US10392621B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3049523B1 (en) | 2019-08-07 |
| CA2924987A1 (en) | 2015-04-02 |
| MX361451B (es) | 2018-12-06 |
| WO2015048084A1 (en) | 2015-04-02 |
| US20180187197A1 (en) | 2018-07-05 |
| SG11201602063UA (en) | 2016-04-28 |
| US20160237435A1 (en) | 2016-08-18 |
| BR112016005957A2 (pt) | 2017-09-26 |
| EP3049523A1 (en) | 2016-08-03 |
| CN114703193A (zh) | 2022-07-05 |
| US9926566B2 (en) | 2018-03-27 |
| KR20160058777A (ko) | 2016-05-25 |
| HK1218765A1 (zh) | 2017-03-10 |
| IL244284B (en) | 2019-12-31 |
| CN105555957A (zh) | 2016-05-04 |
| IL244284A0 (en) | 2016-04-21 |
| US10392621B2 (en) | 2019-08-27 |
| AU2014326975B2 (en) | 2020-05-07 |
| JP6506768B2 (ja) | 2019-04-24 |
| MX2016003320A (es) | 2016-05-31 |
| KR102348283B1 (ko) | 2022-01-10 |
| EP3049523A4 (en) | 2017-05-17 |
| AU2014326975A2 (en) | 2017-01-05 |
| JP2016531593A (ja) | 2016-10-13 |
| AU2014326975A1 (en) | 2016-04-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20190618 |
|
| EEER | Examination request |
Effective date: 20190618 |
|
| EEER | Examination request |
Effective date: 20190618 |