CA2910019A1 - Nanopore-based nucleic acid analysis with mixed fret detection - Google Patents
Nanopore-based nucleic acid analysis with mixed fret detectionInfo
- Publication number
- CA2910019A1 CA2910019A1 CA2910019A CA2910019A CA2910019A1 CA 2910019 A1 CA2910019 A1 CA 2910019A1 CA 2910019 A CA2910019 A CA 2910019A CA 2910019 A CA2910019 A CA 2910019A CA 2910019 A1 CA2910019 A1 CA 2910019A1
- Authority
- CA
- Canada
- Prior art keywords
- nanopore
- fret
- polynucleotide
- nucleotides
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract description 56
- 102000039446 nucleic acids Human genes 0.000 title abstract description 54
- 108020004707 nucleic acids Proteins 0.000 title abstract description 54
- 238000001514 detection method Methods 0.000 title abstract description 21
- 238000004458 analytical method Methods 0.000 title abstract description 8
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 128
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 128
- 239000002157 polynucleotide Substances 0.000 claims abstract description 128
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 121
- 239000002773 nucleotide Substances 0.000 claims abstract description 116
- 238000000034 method Methods 0.000 claims abstract description 64
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims abstract 57
- 108090000623 proteins and genes Proteins 0.000 claims description 93
- 102000004169 proteins and genes Human genes 0.000 claims description 92
- 239000000370 acceptor Substances 0.000 claims description 81
- 239000012528 membrane Substances 0.000 claims description 50
- 239000007790 solid phase Substances 0.000 claims description 31
- 239000002096 quantum dot Substances 0.000 claims description 26
- 238000002372 labelling Methods 0.000 claims description 15
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 abstract description 50
- 230000003287 optical effect Effects 0.000 abstract description 21
- 235000018102 proteins Nutrition 0.000 description 84
- 239000011148 porous material Substances 0.000 description 40
- 230000005945 translocation Effects 0.000 description 32
- 108020004414 DNA Proteins 0.000 description 30
- 239000000758 substrate Substances 0.000 description 27
- 239000000178 monomer Substances 0.000 description 25
- 239000007787 solid Substances 0.000 description 22
- 239000000463 material Substances 0.000 description 21
- 108091034117 Oligonucleotide Proteins 0.000 description 18
- 108010013381 Porins Proteins 0.000 description 18
- 102000017033 Porins Human genes 0.000 description 18
- 238000012986 modification Methods 0.000 description 17
- 230000004048 modification Effects 0.000 description 17
- 150000002632 lipids Chemical class 0.000 description 16
- 238000010884 ion-beam technique Methods 0.000 description 15
- 230000003993 interaction Effects 0.000 description 14
- 108091006146 Channels Proteins 0.000 description 12
- 229910052770 Uranium Inorganic materials 0.000 description 12
- 238000012546 transfer Methods 0.000 description 12
- 239000012491 analyte Substances 0.000 description 11
- 239000010410 layer Substances 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 229910052581 Si3N4 Inorganic materials 0.000 description 10
- 230000005684 electric field Effects 0.000 description 10
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 10
- 102000053602 DNA Human genes 0.000 description 9
- 230000002209 hydrophobic effect Effects 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 235000001014 amino acid Nutrition 0.000 description 8
- 150000001413 amino acids Chemical class 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 230000027455 binding Effects 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 239000000232 Lipid Bilayer Substances 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 7
- 238000000576 coating method Methods 0.000 description 7
- 239000000975 dye Substances 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 150000003573 thiols Chemical class 0.000 description 7
- 101710092462 Alpha-hemolysin Proteins 0.000 description 6
- 239000004971 Cross linker Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
- 235000018417 cysteine Nutrition 0.000 description 6
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 6
- 238000000151 deposition Methods 0.000 description 6
- 230000008021 deposition Effects 0.000 description 6
- 238000007672 fourth generation sequencing Methods 0.000 description 6
- 239000001307 helium Substances 0.000 description 6
- 229910052734 helium Inorganic materials 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 150000002500 ions Chemical class 0.000 description 6
- 239000012038 nucleophile Substances 0.000 description 6
- 150000003141 primary amines Chemical group 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 229910004613 CdTe Inorganic materials 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 4
- 150000001540 azides Chemical class 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 239000002041 carbon nanotube Substances 0.000 description 4
- 229910021393 carbon nanotube Inorganic materials 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 125000000524 functional group Chemical group 0.000 description 4
- -1 helium ion Chemical class 0.000 description 4
- 239000003228 hemolysin Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 150000001282 organosilanes Chemical class 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000004593 Epoxy Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 150000001345 alkine derivatives Chemical class 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000003491 array Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000007844 bleaching agent Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical class O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 3
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 125000005647 linker group Chemical group 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 229910000077 silane Inorganic materials 0.000 description 3
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical class CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 3
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
- CKTSBUTUHBMZGZ-ULQXZJNLSA-N 4-amino-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-tritiopyrimidin-2-one Chemical compound O=C1N=C(N)C([3H])=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-ULQXZJNLSA-N 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 101000945318 Homo sapiens Calponin-1 Proteins 0.000 description 2
- 101000652736 Homo sapiens Transgelin Proteins 0.000 description 2
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical group NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 2
- 235000019687 Lamb Nutrition 0.000 description 2
- 108010021466 Mutant Proteins Proteins 0.000 description 2
- 102000008300 Mutant Proteins Human genes 0.000 description 2
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical group BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 108700028353 OmpC Proteins 0.000 description 2
- 108700006385 OmpF Proteins 0.000 description 2
- 101710129178 Outer plastidial membrane protein porin Proteins 0.000 description 2
- OFBQJSOFQDEBGM-UHFFFAOYSA-N Pentane Chemical compound CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- 102100031013 Transgelin Human genes 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- 102100037820 Voltage-dependent anion-selective channel protein 1 Human genes 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 230000008827 biological function Effects 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 150000001732 carboxylic acid derivatives Chemical group 0.000 description 2
- 229910052681 coesite Inorganic materials 0.000 description 2
- 229910052906 cristobalite Inorganic materials 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000005553 drilling Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008151 electrolyte solution Substances 0.000 description 2
- 238000000295 emission spectrum Methods 0.000 description 2
- 102000034287 fluorescent proteins Human genes 0.000 description 2
- 108091006047 fluorescent proteins Proteins 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 238000013412 genome amplification Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000012948 isocyanate Substances 0.000 description 2
- 150000002513 isocyanates Chemical class 0.000 description 2
- 108040007791 maltose transporting porin activity proteins Proteins 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 125000003835 nucleoside group Chemical group 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
- 239000000985 reactive dye Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 150000004756 silanes Chemical class 0.000 description 2
- 125000005372 silanol group Chemical group 0.000 description 2
- 235000012239 silicon dioxide Nutrition 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 229910052682 stishovite Inorganic materials 0.000 description 2
- JJAHTWIKCUJRDK-UHFFFAOYSA-N succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate Chemical compound C1CC(CN2C(C=CC2=O)=O)CCC1C(=O)ON1C(=O)CCC1=O JJAHTWIKCUJRDK-UHFFFAOYSA-N 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 229910052905 tridymite Inorganic materials 0.000 description 2
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 description 1
- GVJXGCIPWAVXJP-UHFFFAOYSA-N 2,5-dioxo-1-oxoniopyrrolidine-3-sulfonate Chemical compound ON1C(=O)CC(S(O)(=O)=O)C1=O GVJXGCIPWAVXJP-UHFFFAOYSA-N 0.000 description 1
- 150000003923 2,5-pyrrolediones Chemical group 0.000 description 1
- CRYCZDRIXVHNQB-UHFFFAOYSA-N 2-amino-8-bromo-3,7-dihydropurin-6-one Chemical compound N1C(N)=NC(=O)C2=C1N=C(Br)N2 CRYCZDRIXVHNQB-UHFFFAOYSA-N 0.000 description 1
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 1
- FVXHPCVBOXMRJP-UHFFFAOYSA-N 8-bromo-7h-purin-6-amine Chemical compound NC1=NC=NC2=C1NC(Br)=N2 FVXHPCVBOXMRJP-UHFFFAOYSA-N 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000252506 Characiformes Species 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 229920004943 Delrin® Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000001327 Förster resonance energy transfer Methods 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 241000055890 Gorceixia Species 0.000 description 1
- 108010006464 Hemolysin Proteins Proteins 0.000 description 1
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241000283283 Orcinus orca Species 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000001335 aliphatic alkanes Chemical group 0.000 description 1
- 125000003545 alkoxy group Chemical group 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000012769 bulk production Methods 0.000 description 1
- 150000001735 carboxylic acids Chemical group 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005229 chemical vapour deposition Methods 0.000 description 1
- GZGREZWGCWVAEE-UHFFFAOYSA-N chloro-dimethyl-octadecylsilane Chemical compound CCCCCCCCCCCCCCCCCC[Si](C)(C)Cl GZGREZWGCWVAEE-UHFFFAOYSA-N 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- NZNMSOFKMUBTKW-UHFFFAOYSA-N cyclohexanecarboxylic acid Chemical compound OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 229960003964 deoxycholic acid Drugs 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002148 esters Chemical group 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- CKHJYUSOUQDYEN-UHFFFAOYSA-N gallium(3+) Chemical compound [Ga+3] CKHJYUSOUQDYEN-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002411 histidines Chemical class 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- DCPMPXBYPZGNDC-UHFFFAOYSA-N hydron;methanediimine;chloride Chemical compound Cl.N=C=N DCPMPXBYPZGNDC-UHFFFAOYSA-N 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 229910052754 neon Inorganic materials 0.000 description 1
- GKAOGPIIYCISHV-UHFFFAOYSA-N neon atom Chemical compound [Ne] GKAOGPIIYCISHV-UHFFFAOYSA-N 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 230000000269 nucleophilic effect Effects 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Substances [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 150000003230 pyrimidines Chemical class 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000002444 silanisation Methods 0.000 description 1
- SCPYDCQAZCOKTP-UHFFFAOYSA-N silanol Chemical compound [SiH3]O SCPYDCQAZCOKTP-UHFFFAOYSA-N 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000004557 single molecule detection Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
- 238000004544 sputter deposition Methods 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 125000004149 thio group Chemical group *S* 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 238000001947 vapour-phase growth Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361827519P | 2013-05-24 | 2013-05-24 | |
| US61/827,519 | 2013-05-24 | ||
| PCT/US2014/039444 WO2014190322A2 (en) | 2013-05-24 | 2014-05-23 | Nanopore-based nucleic acid analysis with mixed fret detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2910019A1 true CA2910019A1 (en) | 2014-11-27 |
Family
ID=51934368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2910019A Abandoned CA2910019A1 (en) | 2013-05-24 | 2014-05-23 | Nanopore-based nucleic acid analysis with mixed fret detection |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US9862997B2 (enExample) |
| EP (1) | EP3004385B1 (enExample) |
| JP (2) | JP6399610B2 (enExample) |
| CN (1) | CN105283560B (enExample) |
| AU (1) | AU2014268322B2 (enExample) |
| CA (1) | CA2910019A1 (enExample) |
| WO (1) | WO2014190322A2 (enExample) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| US9651539B2 (en) | 2012-10-28 | 2017-05-16 | Quantapore, Inc. | Reducing background fluorescence in MEMS materials by low energy ion beam treatment |
| EP3004385B1 (en) | 2013-05-24 | 2018-11-28 | Quantapore Inc. | Nanopore-based nucleic acid analysis with mixed fret detection |
| ES2789000T3 (es) | 2014-10-10 | 2020-10-23 | Quantapore Inc | Análisis de polinucleótidos basado en nanoporos con marcadores fluorescentes que se inactivan mutuamente |
| CA2964790C (en) | 2014-10-24 | 2023-02-21 | Quantapore, Inc. | Efficient optical analysis of polymers using arrays of nanostructures |
| WO2016200737A1 (en) * | 2015-06-09 | 2016-12-15 | Quantapore, Inc. | Nanopore-based analysis of compounds using mobile fret pairs |
| EP3387108B1 (en) * | 2015-12-08 | 2022-05-25 | Quantapore, Inc. | Method of translocating nucleic acids through nanopores |
| JP6967006B2 (ja) * | 2016-01-15 | 2021-11-17 | クアンタポール， インコーポレイテッド | 低減されたバックグラウンドの光学に基づくナノポア分析 |
| EP3443122B1 (en) * | 2016-04-14 | 2021-07-07 | Quantapore, Inc. | Mixed optical signals in polymer analysis with nanopores |
| EP3464612B8 (en) | 2016-05-31 | 2024-12-11 | Switchback Systems, Inc. | Two-color nanopore sequencing |
| US10823721B2 (en) | 2016-07-05 | 2020-11-03 | Quantapore, Inc. | Optically based nanopore sequencing |
| JP2019528065A (ja) | 2016-08-19 | 2019-10-10 | クアンタポール， インコーポレイテッド | 消光剤を使用する光学に基づくナノポア配列決定 |
| CN109804085A (zh) * | 2016-10-10 | 2019-05-24 | 昆塔波尔公司 | 在基于光学的纳米孔测序中控制荧光信号组成 |
| MX2018013686A (es) * | 2016-10-24 | 2019-05-02 | Two Pore Guys Inc | Abundancia fraccional de secuencias de polinucleotidos en una muestra. |
| WO2019081442A1 (en) | 2017-10-23 | 2019-05-02 | F. Hoffmann-La Roche Ag | REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE |
| AU2018358057B2 (en) | 2017-10-31 | 2023-03-02 | Encodia, Inc. | Kits for analysis using nucleic acid encoding and/or label |
| IL278769B2 (en) | 2018-05-17 | 2025-06-01 | Stuart Lindsay | Device, system and method for direct electrical measurement of enzyme activity |
| EP4273550A3 (en) | 2018-05-17 | 2024-04-03 | Northeastern University | Lipid-free anchoring of thermophilic bacteriophage g20c portal adapter into solid-state nanopores |
| CN113166209B (zh) | 2018-10-08 | 2025-01-07 | 弗里堡大学阿道夫梅克尔研究所 | 成孔肽的基于寡核苷酸的调节以增加孔尺寸、膜亲和性、稳定性和抗菌活性 |
| DE102019200929A1 (de) * | 2019-01-25 | 2020-07-30 | Robert Bosch Gmbh | Verfahren und Vorrichtung zum optischen Erfassen einer Länge eines Makromoleküls |
| AU2020214334A1 (en) | 2019-01-30 | 2021-08-12 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic circuits, systems and methods for preparing and using them |
| CN110243792A (zh) * | 2019-06-11 | 2019-09-17 | 山东师范大学 | 一种基于量子点和四面体dna结构的荧光化学传感器及其检测方法和应用 |
| CN110736775A (zh) * | 2019-10-11 | 2020-01-31 | 深圳清华大学研究院 | 一种固态纳米孔修饰处理方法 |
| AU2021263432A1 (en) | 2020-04-30 | 2022-12-01 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for sequencing biopolymers |
| EP4102210A1 (en) * | 2021-06-07 | 2022-12-14 | Oxford University Innovation Limited | Nanophotonic nanopore, memory system comprising a nanophotonic nanopore, and method of reading and/or writing information from and/or to an analyte |
| US12624389B2 (en) | 2021-06-07 | 2026-05-12 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for sequencing biopolymers |
| CN113533275B (zh) * | 2021-06-30 | 2023-11-28 | 东南大学 | 固态纳米孔-荧光共振能量转移复合检测方法及系统 |
Family Cites Families (211)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH614781A5 (enExample) | 1977-06-27 | 1979-12-14 | Contraves Ag | |
| US5821058A (en) | 1984-01-16 | 1998-10-13 | California Institute Of Technology | Automated DNA sequencing technique |
| US4962037A (en) | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
| US5131755A (en) | 1988-02-19 | 1992-07-21 | Chadwick Curt H | Automatic high speed optical inspection system |
| JP3123249B2 (ja) | 1991-09-10 | 2001-01-09 | 株式会社日立製作所 | Ｄｎａ分子の長さ計測方法および計測装置 |
| US5405747A (en) | 1991-09-25 | 1995-04-11 | The Regents Of The University Of California Office Of Technology Transfer | Method for rapid base sequencing in DNA and RNA with two base labeling |
| DE69322266T2 (de) | 1992-04-03 | 1999-06-02 | Perkin-Elmer Corp., Foster City, Calif. | Proben zusammensetzung und verfahren |
| US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
| US5387926A (en) | 1992-06-30 | 1995-02-07 | California Institute Of Technology | High speed digital framing camera |
| US5798042A (en) | 1994-03-07 | 1998-08-25 | Regents Of The University Of California | Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters |
| US5624845A (en) * | 1995-03-16 | 1997-04-29 | International Business Machines Corporation | Assembly and a method suitable for identifying a code |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6794127B1 (en) | 1997-06-16 | 2004-09-21 | Diversa Corporation | Capillary array-based sample screening |
| EP0910664A1 (en) | 1996-04-15 | 1999-04-28 | University Of Southern California | Synthesis of fluorophore-labeled dna |
| US6399023B1 (en) | 1996-04-16 | 2002-06-04 | Caliper Technologies Corp. | Analytical system and method |
| US5945526A (en) | 1996-05-03 | 1999-08-31 | Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| US6136543A (en) | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
| EP1009802B1 (en) | 1997-02-12 | 2004-08-11 | Eugene Y. Chan | Methods for analyzimg polymers |
| DE19714221A1 (de) | 1997-04-07 | 1998-10-08 | Zeiss Carl Fa | Konfokales Mikroskop mit einem motorischen Scanningtisch |
| CZ297165B6 (cs) | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru |
| WO1999005167A1 (en) | 1997-07-25 | 1999-02-04 | University Of Massachusetts | Designed protein pores as components for biosensors |
| WO1999028505A1 (en) | 1997-12-03 | 1999-06-10 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US6758961B1 (en) | 1997-12-17 | 2004-07-06 | Ecole Polytechnique Federale De Lausanne | Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers |
| US7244349B2 (en) | 1997-12-17 | 2007-07-17 | Molecular Devices Corporation | Multiaperture sample positioning and analysis system |
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
| ATE202711T1 (de) | 1997-12-23 | 2001-07-15 | Steris Corp | Anordnung zur freisetzung einer antimikrobiellen zusammensetzung mit integriertem filter |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| DE19804372A1 (de) | 1998-02-04 | 1999-08-05 | Michael W Dr Dr Dahm | Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits |
| GB9805301D0 (en) | 1998-03-12 | 1998-05-06 | Imperial College | Detector |
| US6306589B1 (en) | 1998-05-27 | 2001-10-23 | Vysis, Inc. | Biological assays for analyte detection |
| US6504943B1 (en) | 1998-07-20 | 2003-01-07 | Sandia Corporation | Information-efficient spectral imaging sensor |
| US6447724B1 (en) | 1998-08-11 | 2002-09-10 | Caliper Technologies Corp. | DNA sequencing using multiple fluorescent labels being distinguishable by their decay times |
| US6790671B1 (en) | 1998-08-13 | 2004-09-14 | Princeton University | Optically characterizing polymers |
| US6210896B1 (en) | 1998-08-13 | 2001-04-03 | Us Genomics | Molecular motors |
| US6263286B1 (en) | 1998-08-13 | 2001-07-17 | U.S. Genomics, Inc. | Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer |
| US6906749B1 (en) | 1998-09-16 | 2005-06-14 | Dalsa, Inc. | CMOS TDI image sensor |
| EP2306195A3 (en) | 1998-09-18 | 2012-04-25 | Massachusetts Institute of Technology | Biological applications of semiconductor nanocrystals |
| US6251303B1 (en) | 1998-09-18 | 2001-06-26 | Massachusetts Institute Of Technology | Water-soluble fluorescent nanocrystals |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
| US6252303B1 (en) | 1998-12-02 | 2001-06-26 | Advanced Micro Devices, Inc. | Intergration of low-K SiOF as inter-layer dielectric |
| WO2000034527A2 (en) | 1998-12-11 | 2000-06-15 | The Regents Of The University Of California | Targeted molecular bar codes |
| FR2788008B1 (fr) | 1998-12-30 | 2001-03-23 | Inst Curie | Milieu thermosensible pour la separation electrocinetique d'especes au sein d'un canal de separation |
| US6455682B1 (en) | 1999-01-20 | 2002-09-24 | Northwestern University | DNA mobility modifier |
| US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
| US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6249341B1 (en) | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6975400B2 (en) | 1999-01-25 | 2005-12-13 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| JP2003527075A (ja) | 1999-03-25 | 2003-09-16 | ハイセック，インコーポレーテッド | ハイブリダイゼーションによる配列分析のための、溶液ベースの方法 |
| US20040214221A1 (en) | 1999-05-07 | 2004-10-28 | Klaus Muehlegger | High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used |
| US7122152B2 (en) | 1999-05-10 | 2006-10-17 | University Of Florida | Spatiotemporal and geometric optimization of sensor arrays for detecting analytes fluids |
| US7056661B2 (en) | 1999-05-19 | 2006-06-06 | Cornell Research Foundation, Inc. | Method for sequencing nucleic acid molecules |
| US8206568B2 (en) | 1999-06-22 | 2012-06-26 | President And Fellows Of Harvard College | Material deposition techniques for control of solid state aperture surface properties |
| AU5631200A (en) | 1999-06-22 | 2001-01-09 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
| US6818395B1 (en) | 1999-06-28 | 2004-11-16 | California Institute Of Technology | Methods and apparatus for analyzing polynucleotide sequences |
| US6982146B1 (en) | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| AU7105300A (en) | 1999-09-03 | 2001-04-10 | Lifebeam Technologies, Inc. | Optical system for rapid polymer analysis |
| CA2384100C (en) | 1999-09-07 | 2011-03-29 | The Regents Of The University Of California | Methods of determining the presence of double stranded nucleic acids in a sample |
| US6335420B1 (en) | 1999-11-22 | 2002-01-01 | Ibc Advanced Technologies, Inc. | Polyamide ligand-containing polymeric resins and methods of using the same for removing, separating and/or concentrating desired metal ions from solutions |
| WO2001055454A1 (en) | 2000-01-28 | 2001-08-02 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| EP2083015B1 (en) | 2000-02-11 | 2016-04-06 | The Texas A & M University System | Biosensor compositions and methods of use |
| US20060292041A1 (en) | 2000-03-23 | 2006-12-28 | Dugas Matthew P | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6616895B2 (en) | 2000-03-23 | 2003-09-09 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| US6413792B1 (en) | 2000-04-24 | 2002-07-02 | Eagle Research Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6917726B2 (en) | 2001-09-27 | 2005-07-12 | Cornell Research Foundation, Inc. | Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes |
| US20050153284A1 (en) | 2000-06-30 | 2005-07-14 | Zeno Foldes-Papp | Single molecule sequencing method |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
| US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
| US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
| WO2002018951A2 (en) | 2000-08-29 | 2002-03-07 | The Rockefeller University | Methods employing fluorescence quenching by metal surfaces |
| WO2002042496A2 (en) | 2000-11-27 | 2002-05-30 | The Regents Of The University Of California | Methods and devices for characterizing duplex nucleic acid molecules |
| US6998251B2 (en) | 2001-01-12 | 2006-02-14 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| US6743905B2 (en) | 2001-04-16 | 2004-06-01 | Applera Corporation | Mobility-modified nucleobase polymers and methods of using same |
| US7285010B2 (en) | 2001-05-15 | 2007-10-23 | Ebara Corporation | TDI detecting device, a feed-through equipment and electron beam apparatus using these devices |
| WO2002094846A2 (en) | 2001-05-22 | 2002-11-28 | Parallel Synthesis Technologies, Inc. | Method for in situ, on-chip chemical synthesis |
| US6618140B2 (en) | 2001-06-18 | 2003-09-09 | Amnis Corporation | Spectral deconvolution of fluorescent markers |
| US7009163B2 (en) | 2001-06-22 | 2006-03-07 | Orbotech Ltd. | High-sensitivity optical scanning using memory integration |
| EP1410031A2 (en) | 2001-06-28 | 2004-04-21 | ADVANCED RESEARCH AND TECHNOLOGY INSTITUTE, Inc. | Methods of preparing multicolor quantum dot tagged beads and conjugates thereof |
| EP1412724B1 (en) | 2001-07-25 | 2016-03-23 | Life Technologies Corporation | Time-delay integration in electrophoretic detection systems |
| WO2003010289A2 (en) | 2001-07-25 | 2003-02-06 | The Trustees Of Princeton University | Nanochannel arrays and their preparation and use for high throughput macromolecular analysis |
| US7280207B2 (en) | 2001-07-25 | 2007-10-09 | Applera Corporation | Time-delay integration in a flow cytometry system |
| US6627433B2 (en) | 2001-08-24 | 2003-09-30 | Applera Corporation | Multi-channel analyte-separation device employing side-entry excitation |
| US20080288178A1 (en) | 2001-08-24 | 2008-11-20 | Applera Corporation | Sequencing system with memory |
| US6852492B2 (en) | 2001-09-24 | 2005-02-08 | Intel Corporation | Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication |
| US6706203B2 (en) | 2001-10-30 | 2004-03-16 | Agilent Technologies, Inc. | Adjustable nanopore, nanotome, and nanotweezer |
| DE10154291B4 (de) | 2001-11-05 | 2005-05-19 | Hain Lifescience Gmbh | Verfahren zum Nachweis von Nukleinsäuren in Form eines Trockenschnelltestes |
| DE10162535A1 (de) | 2001-12-19 | 2003-07-03 | Gnothis Holding Sa Ecublens | Sequenzierung über Lochmembranen |
| US7008547B2 (en) | 2002-03-14 | 2006-03-07 | Sarnoff Corporation | Solid phase sensors |
| JP3752466B2 (ja) | 2002-04-24 | 2006-03-08 | 株式会社日立製作所 | 遺伝子検査方法 |
| US7744816B2 (en) | 2002-05-01 | 2010-06-29 | Intel Corporation | Methods and device for biomolecule characterization |
| US8278055B2 (en) | 2002-05-01 | 2012-10-02 | Intel Corporation | Methods and device for analyte characterization |
| AU2003245272A1 (en) | 2002-05-10 | 2003-11-11 | The Texas A And M University System | Stochastic sensing through covalent interactions |
| US20050019784A1 (en) | 2002-05-20 | 2005-01-27 | Xing Su | Method and apparatus for nucleic acid sequencing and identification |
| US7005264B2 (en) | 2002-05-20 | 2006-02-28 | Intel Corporation | Method and apparatus for nucleic acid sequencing and identification |
| US6952651B2 (en) | 2002-06-17 | 2005-10-04 | Intel Corporation | Methods and apparatus for nucleic acid sequencing by signal stretching and data integration |
| TWI234584B (en) | 2002-08-16 | 2005-06-21 | Univ Nat Taiwan Normal | Nucleic acid sequencing method |
| EP1563289A4 (en) | 2002-09-25 | 2009-11-11 | Ge Healthcare Sv Corp | HIGH DENSITY FLUIDIC CHIP DESIGN AND SAMPLE INJECTION METHOD |
| AU2003301244A1 (en) | 2002-10-15 | 2004-05-04 | Advanced Research Corporation | Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples |
| CN1161613C (zh) | 2002-10-22 | 2004-08-11 | 中国人民解放军南京军区联勤部军事医学研究所 | 免疫细胞微流体阵列 |
| US7390457B2 (en) | 2002-10-31 | 2008-06-24 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| US20040137158A1 (en) | 2003-01-15 | 2004-07-15 | Kools Jacques Constant Stefan | Method for preparing a noble metal surface |
| US7410564B2 (en) | 2003-01-27 | 2008-08-12 | Agilent Technologies, Inc. | Apparatus and method for biopolymer identification during translocation through a nanopore |
| CA2513889A1 (en) | 2003-01-29 | 2004-08-19 | 454 Corporation | Double ended sequencing |
| AU2004223493A1 (en) | 2003-02-28 | 2004-10-07 | Brown University | Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same |
| US7250115B2 (en) | 2003-06-12 | 2007-07-31 | Agilent Technologies, Inc | Nanopore with resonant tunneling electrodes |
| US7248771B2 (en) | 2003-06-16 | 2007-07-24 | Brigham Young University | Integrated sensor with electrical and optical single molecule sensitivity |
| US7444053B2 (en) | 2003-06-16 | 2008-10-28 | The Regents Of The University Of California | Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity |
| WO2005017025A2 (en) | 2003-08-15 | 2005-02-24 | The President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7075161B2 (en) | 2003-10-23 | 2006-07-11 | Agilent Technologies, Inc. | Apparatus and method for making a low capacitance artificial nanopore |
| US20050095599A1 (en) | 2003-10-30 | 2005-05-05 | Pittaro Richard J. | Detection and identification of biopolymers using fluorescence quenching |
| US20050136408A1 (en) | 2003-12-19 | 2005-06-23 | May Tom-Moy | Methods and systems for characterizing a polymer |
| US7271896B2 (en) | 2003-12-29 | 2007-09-18 | Intel Corporation | Detection of biomolecules using porous biosensors and raman spectroscopy |
| US20050164211A1 (en) | 2004-01-22 | 2005-07-28 | Hannah Eric C. | Carbon nanotube molecular labels |
| CA2552858A1 (en) | 2004-01-23 | 2005-08-04 | Lingvitae As | Improving polynucleotide ligation reactions |
| US20050186576A1 (en) | 2004-02-19 | 2005-08-25 | Intel Corporation | Polymer sequencing using selectively labeled monomers and data integration |
| US7279337B2 (en) | 2004-03-10 | 2007-10-09 | Agilent Technologies, Inc. | Method and apparatus for sequencing polymers through tunneling conductance variation detection |
| WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US20050227239A1 (en) | 2004-04-08 | 2005-10-13 | Joyce Timothy H | Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes |
| US20070264623A1 (en) | 2004-06-15 | 2007-11-15 | President And Fellows Of Harvard College | Nanosensors |
| US20060019259A1 (en) | 2004-07-22 | 2006-01-26 | Joyce Timothy H | Characterization of biopolymers by resonance tunneling and fluorescence quenching |
| GB2431013B (en) | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| US20070054276A1 (en) | 2004-08-12 | 2007-03-08 | Sampson Jeffrey R | Polynucleotide analysis and methods of using nanopores |
| CA2577079C (en) | 2004-08-13 | 2014-05-20 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore dna readout platform |
| US20060062531A1 (en) | 2004-09-17 | 2006-03-23 | Stephen Turner | Fabrication of optical confinements |
| WO2006137891A2 (en) | 2004-09-29 | 2006-12-28 | University Of Florida Research Foundation, Inc. | Membrane with nanochannels for detection of molecules |
| US7371533B2 (en) | 2004-10-05 | 2008-05-13 | University Of Ottawa | Methods for separation of polymeric compounds |
| WO2006052882A1 (en) | 2004-11-09 | 2006-05-18 | President And Fellows Of Harvard College | Formation of eddies in constrained fluidic channels and uses thereof |
| US7609309B2 (en) | 2004-11-18 | 2009-10-27 | Kla-Tencor Technologies Corporation | Continuous clocking of TDI sensors |
| US7220549B2 (en) | 2004-12-30 | 2007-05-22 | Helicos Biosciences Corporation | Stabilizing a nucleic acid for nucleic acid sequencing |
| US20060210995A1 (en) | 2005-03-15 | 2006-09-21 | Joyce Timothy H | Nanopore analysis systems and methods of using nanopore devices |
| EP1877762B1 (en) | 2005-04-06 | 2011-10-19 | President and Fellows of Harvard College | Molecular characterization with carbon nanotube control |
| US20060231419A1 (en) | 2005-04-15 | 2006-10-19 | Barth Philip W | Molecular resonant tunneling sensor and methods of fabricating and using the same |
| WO2006116683A1 (en) | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescence |
| EP1721657A1 (en) | 2005-05-13 | 2006-11-15 | SONY DEUTSCHLAND GmbH | A method of fabricating a polymeric membrane having at least one pore |
| JP2007006720A (ja) | 2005-06-28 | 2007-01-18 | Toshiba Corp | 個体識別方法、並びに個体識別検査のためのアレイ、装置及びシステム |
| WO2007021814A2 (en) | 2005-08-11 | 2007-02-22 | Eksigent Technologies, Llc | Plastic surfaces and apparatuses for reduced adsorption of solutes and methods of preparing the same |
| US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
| WO2007041340A2 (en) | 2005-09-30 | 2007-04-12 | The Trustees Of Columbia University In The City Of New York | Microfluidic cells with parallel arrays of individual dna molecules |
| WO2007041621A2 (en) | 2005-10-03 | 2007-04-12 | Xingsheng Sean Ling | Hybridization assisted nanopore sequencing |
| KR100721430B1 (ko) | 2005-10-12 | 2007-05-23 | 학교법인 포항공과대학교 | 나노다공성 멤브레인 및 이의 제조방법 |
| US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
| US7835870B2 (en) | 2005-11-01 | 2010-11-16 | Georgia Institute Of Technology | Methods and systems for evaluating the length of elongated elements |
| US20070190543A1 (en) | 2005-11-14 | 2007-08-16 | Applera Corporation | Coded Molecules for Detecting Target Analytes |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| ATE481505T1 (de) | 2005-12-12 | 2010-10-15 | Us Gov Health & Human Serv | Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren |
| JP2009526969A (ja) | 2006-02-13 | 2009-07-23 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 分子診断アプリケーションのための微小流体装置 |
| EP1994179A2 (en) | 2006-02-18 | 2008-11-26 | Michael Strathmann | Massively multiplexed sequencing |
| US20070202008A1 (en) | 2006-02-28 | 2007-08-30 | Schembri Carol T | Systems and methods of lipoprotein size fraction assaying |
| US20070215472A1 (en) | 2006-03-15 | 2007-09-20 | Slater Gary W | Electroosmotic flow for end labelled free solution electrophoresis |
| US20070218494A1 (en) | 2006-03-17 | 2007-09-20 | Gary Slater | Branched polymer lables as drag-tags in free solution electrophoresis |
| US7777505B2 (en) | 2006-05-05 | 2010-08-17 | University Of Utah Research Foundation | Nanopore platforms for ion channel recordings and single molecule detection and analysis |
| US7849581B2 (en) | 2006-05-05 | 2010-12-14 | University Of Utah Research Foundation | Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof |
| RU2009102155A (ru) | 2006-06-30 | 2010-08-10 | Апплера Корпорейшн (Us) | Способы анализирования связывающих взаимодействий |
| US7553730B2 (en) | 2006-07-14 | 2009-06-30 | Agilent Technologies, Inc. | Methods of fabrication employing nanoscale mandrels |
| US20080032290A1 (en) | 2006-08-03 | 2008-02-07 | Young James E | Nanopore flow cells |
| EP2074211B1 (en) | 2006-09-06 | 2013-03-13 | Medical Research Council | DNA polymerases incorporating dye-labelled nucleotide analogues |
| WO2008049795A1 (en) | 2006-10-23 | 2008-05-02 | Flexgen Bv | Method and system for calibrating laser focus and position in micro-arrays |
| DE112007002932B4 (de) | 2006-12-01 | 2015-08-06 | The Trustees Of Columbia University In The City Of New York | Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren |
| US7544578B2 (en) | 2007-01-03 | 2009-06-09 | International Business Machines Corporation | Structure and method for stochastic integrated circuit personalization |
| US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
| EP2126072A4 (en) | 2007-02-21 | 2011-07-13 | Life Technologies Corp | MATERIALS AND METHOD FOR THE INDIVIDUAL MOLECULE SEQUENCING OF NUCLEIC ACIDS |
| US20080254995A1 (en) | 2007-02-27 | 2008-10-16 | Drexel University | Nanopore arrays and sequencing devices and methods thereof |
| WO2008124107A1 (en) | 2007-04-04 | 2008-10-16 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| US8182993B2 (en) | 2007-06-06 | 2012-05-22 | Pacific Biosciences Of California, Inc. | Methods and processes for calling bases in sequence by incorporation methods |
| WO2008157696A2 (en) | 2007-06-19 | 2008-12-24 | Stratos Genomics Inc. | High throughput nucleic acid sequencing by expansion |
| GB0713143D0 (en) | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
| GB0717150D0 (en) | 2007-09-04 | 2007-10-17 | Univ Warwick | Apparatus and method |
| EP2205765B1 (en) | 2007-10-02 | 2012-09-12 | President and Fellows of Harvard College | Capture, recapture, and trapping of molecules with a nanopore |
| GB0721340D0 (en) | 2007-10-30 | 2007-12-12 | Isis Innovation | Polymerase-based single-molecule DNA sequencing |
| US20110165652A1 (en) * | 2008-01-14 | 2011-07-07 | Life Technologies Corporation | Compositions, methods and systems for single molecule sequencing |
| WO2009092035A2 (en) | 2008-01-17 | 2009-07-23 | Sequenom, Inc. | Methods and compositions for the analysis of biological molecules |
| GB2473333B (en) | 2008-02-28 | 2011-08-24 | Electronic Bio Sciences Llc | System and method to improve sequencing accuracy of a polymer |
| EP3269824A1 (en) | 2008-03-28 | 2018-01-17 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
| WO2009123767A1 (en) | 2008-04-04 | 2009-10-08 | Life Technologies Corporation | Scanning system and method for imaging and sequencing |
| US20110172404A1 (en) | 2008-05-19 | 2011-07-14 | Cornell University | Self-Assembly of Nanoparticles Through Nuclei Acid Engineering |
| US8013300B2 (en) | 2008-06-20 | 2011-09-06 | Carl Zeiss Nts, Llc | Sample decontamination |
| CA3062190C (en) | 2008-06-30 | 2023-03-28 | Bionano Genomics, Inc. | Methods and devices for single-molecule whole genome analysis |
| WO2010004273A1 (en) | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Base-detecting pore |
| EP2313758B1 (en) | 2008-07-17 | 2012-02-01 | Koninklijke Philips Electronics N.V. | Nanopore device and a method for nucleic acid analysis |
| CN102216783B (zh) | 2008-09-22 | 2015-04-01 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| WO2010036287A1 (en) | 2008-09-24 | 2010-04-01 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
| CN201302544Y (zh) | 2008-12-04 | 2009-09-02 | 浙江大学 | 高俘获率高灵敏度的微流控spr生物传感装置 |
| WO2010080617A2 (en) | 2008-12-19 | 2010-07-15 | The Board Of Trustees Of The University Of Illinois | Detecting and sorting methylated dna using a synthetic nanopore |
| JP5687683B2 (ja) | 2009-03-26 | 2015-03-18 | トラスティーズ オブ ボストン ユニバーシティ | ２種類の流体間の薄い固相界面上における撮像方法 |
| JP5372570B2 (ja) | 2009-03-30 | 2013-12-18 | 株式会社日立ハイテクノロジーズ | ナノポアを用いたバイオポリマー決定方法、システム、及びキット |
| US8986928B2 (en) * | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| WO2010129869A1 (en) | 2009-05-07 | 2010-11-11 | The Trustees Of Boston University | Manufacture of nanoparticles using nanopores and voltage-driven electrolyte flow |
| US8926904B2 (en) | 2009-05-12 | 2015-01-06 | Daniel Wai-Cheong So | Method and apparatus for the analysis and identification of molecules |
| US9255002B2 (en) | 2009-09-22 | 2016-02-09 | Cornell University | Apparatus and method for forming self-assembly arrays |
| US20140335513A9 (en) | 2009-09-30 | 2014-11-13 | Quantapore, Inc. | Hybrid nanopore device with optical detection and methods of using same |
| EP2483680A4 (en) * | 2009-09-30 | 2014-01-01 | Quantapore Inc | ULTRASOUND SEQUENCING OF BIOLOGICAL POLYMERS WITH THE HELP OF A MARKED NANOPORE |
| EP2491138A1 (en) | 2009-10-21 | 2012-08-29 | Bionano Genomics, Inc. | Methods and related devices for single molecule whole genome analysis |
| EP2507387B1 (en) | 2009-12-01 | 2017-01-25 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument and method |
| EP2553125A4 (en) | 2010-03-30 | 2013-10-02 | Univ Boston | TOOLS AND METHOD FOR NANOPORENCE PACKAGING-RELATED NUCLEIC ACID SEQUENCING |
| US20130256118A1 (en) | 2010-05-11 | 2013-10-03 | Trustees Of Boston University | Use of Nanopore Arrays For Multiplex Sequencing of Nucleic Acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| EP2623960B1 (en) | 2010-09-29 | 2020-11-11 | Hitachi High-Tech Corporation | Biopolymer optical analysis device and method |
| WO2012121756A1 (en) | 2011-03-04 | 2012-09-13 | Quantapore, Inc. | Apparatus and methods for performing optical nanopore detection or sequencing |
| US8894796B2 (en) | 2011-06-06 | 2014-11-25 | The University Of North Carolina At Greensboro | Nanopore fabrication and applications thereof |
| US20130040827A1 (en) | 2011-08-14 | 2013-02-14 | Stephen C. Macevicz | Method and compositions for detecting and sequencing nucleic acids |
| GB2497784A (en) | 2011-12-20 | 2013-06-26 | Base4 Innovation Ltd | Identification of polymers using nanopore devices |
| GB201212135D0 (en) | 2012-07-09 | 2012-08-22 | Base4 Innovation Ltd | Improved sequencing apparatus |
| WO2014066905A2 (en) | 2012-10-28 | 2014-05-01 | Quantapore, Inc. | Reducing background fluorescence in mems materials by low energy ion beam treatment |
| EP3004385B1 (en) | 2013-05-24 | 2018-11-28 | Quantapore Inc. | Nanopore-based nucleic acid analysis with mixed fret detection |
| CA2964790C (en) * | 2014-10-24 | 2023-02-21 | Quantapore, Inc. | Efficient optical analysis of polymers using arrays of nanostructures |
-
2014
- 2014-05-23 EP EP14801275.0A patent/EP3004385B1/en active Active
- 2014-05-23 CA CA2910019A patent/CA2910019A1/en not_active Abandoned
- 2014-05-23 WO PCT/US2014/039444 patent/WO2014190322A2/en not_active Ceased
- 2014-05-23 CN CN201480030047.0A patent/CN105283560B/zh active Active
- 2014-05-23 JP JP2016515132A patent/JP6399610B2/ja active Active
- 2014-05-23 AU AU2014268322A patent/AU2014268322B2/en active Active
- 2014-05-23 US US14/786,518 patent/US9862997B2/en active Active
-
2017
- 2017-11-28 US US15/824,885 patent/US20180073072A1/en not_active Abandoned
-
2018
- 2018-03-30 JP JP2018067384A patent/JP2018099141A/ja active Pending
-
2019
- 2019-05-24 US US16/422,675 patent/US20190360035A1/en not_active Abandoned
-
2020
- 2020-08-06 US US16/987,234 patent/US20210130887A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2018099141A (ja) | 2018-06-28 |
| EP3004385A2 (en) | 2016-04-13 |
| US20160076091A1 (en) | 2016-03-17 |
| US20180073072A1 (en) | 2018-03-15 |
| US20210130887A1 (en) | 2021-05-06 |
| US9862997B2 (en) | 2018-01-09 |
| CN105283560B (zh) | 2018-11-30 |
| WO2014190322A2 (en) | 2014-11-27 |
| AU2014268322B2 (en) | 2019-01-24 |
| EP3004385B1 (en) | 2018-11-28 |
| AU2014268322A1 (en) | 2015-10-29 |
| JP2016524469A (ja) | 2016-08-18 |
| WO2014190322A3 (en) | 2015-01-22 |
| EP3004385A4 (en) | 2017-02-22 |
| US20190360035A1 (en) | 2019-11-28 |
| JP6399610B2 (ja) | 2018-10-10 |
| CN105283560A (zh) | 2016-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20210130887A1 (en) | Nanopore-based nucleic acid analysis with mixed fret detection | |
| US11041197B2 (en) | Efficient optical analysis of polymers using arrays of nanostructures | |
| US20220349000A1 (en) | Nanopore-based polymer analysis with mutually-quenching fluorescent labels | |
| US20250320543A1 (en) | Method of translocating nucleic acids through nanopores | |
| US20140335513A9 (en) | Hybrid nanopore device with optical detection and methods of using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20190115 |
|
| FZDE | Discontinued |
Effective date: 20211112 |