CA2904022C - Glucoamylase variants and polynucleotides encoding same - Google Patents

Glucoamylase variants and polynucleotides encoding same Download PDF

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Publication number
CA2904022C
CA2904022C CA2904022A CA2904022A CA2904022C CA 2904022 C CA2904022 C CA 2904022C CA 2904022 A CA2904022 A CA 2904022A CA 2904022 A CA2904022 A CA 2904022A CA 2904022 C CA2904022 C CA 2904022C
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CA
Canada
Prior art keywords
variant
glucoamylase
substitutions
polypeptide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2904022A
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English (en)
French (fr)
Other versions
CA2904022A1 (en
Inventor
Noriko Tsutsumi
Keiichi Ayabe
Siik KISHISHITA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
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Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CA2904022A1 publication Critical patent/CA2904022A1/en
Application granted granted Critical
Publication of CA2904022C publication Critical patent/CA2904022C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2904022A 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same Active CA2904022C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13165995 2013-04-30
EP13165995.5 2013-04-30
PCT/EP2014/058692 WO2014177546A2 (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
CA2904022A1 CA2904022A1 (en) 2014-11-06
CA2904022C true CA2904022C (en) 2022-04-19

Family

ID=48190353

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2904022A Active CA2904022C (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Country Status (9)

Country Link
US (1) US10550374B2 (https=)
EP (2) EP3372680B1 (https=)
CN (2) CN105164253B (https=)
BR (1) BR112015026972B1 (https=)
CA (1) CA2904022C (https=)
DK (2) DK3372680T3 (https=)
ES (2) ES2674701T3 (https=)
MX (2) MX368655B (https=)
WO (1) WO2014177546A2 (https=)

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EP3712240B1 (en) 2014-02-07 2023-09-27 Novozymes A/S Compositions for producing glucose syrups
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10308963B2 (en) 2015-02-27 2019-06-04 Novozymes A/S Processes of producing ethanol using a fermenting organism
US10676727B2 (en) 2015-06-18 2020-06-09 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
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WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
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CA3070281A1 (en) 2017-08-08 2019-02-14 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
CA3089135A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
US11473109B2 (en) 2018-02-15 2022-10-18 Novozymes A/S Yeast for ethanol production
CA3098718A1 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
BR112021001282A2 (pt) 2018-07-25 2021-04-27 Novozymes A/S levedura expressando enzimas para produção de etanol
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EP4004029A1 (en) 2019-07-26 2022-06-01 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
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AU2020402990A1 (en) 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation
BR112023025624A2 (pt) 2021-06-07 2024-02-27 Novozymes As Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2026008449A2 (en) 2024-07-04 2026-01-08 Novozymes A/S A process for producing a fermentation product and a concentrated protein co-product

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Also Published As

Publication number Publication date
EP3372680A1 (en) 2018-09-12
WO2014177546A2 (en) 2014-11-06
MX2015014537A (es) 2016-02-05
BR112015026972A2 (https=) 2017-09-05
BR112015026972B1 (pt) 2023-05-02
CA2904022A1 (en) 2014-11-06
ES2835313T3 (es) 2021-06-22
EP2992092A2 (en) 2016-03-09
DK2992092T3 (en) 2018-06-25
EP2992092B1 (en) 2018-04-04
US10550374B2 (en) 2020-02-04
CN105164253A (zh) 2015-12-16
US20160152960A1 (en) 2016-06-02
DK3372680T3 (da) 2021-01-11
ES2674701T3 (es) 2018-07-03
MX368655B (es) 2019-10-10
MX2019005158A (es) 2019-08-05
CN105164253B (zh) 2025-11-14
CN121380029A (zh) 2026-01-23
WO2014177546A3 (en) 2014-12-24
EP3372680B1 (en) 2020-11-11

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