CA2904022C - Glucoamylase variants and polynucleotides encoding same - Google Patents

Glucoamylase variants and polynucleotides encoding same Download PDF

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Publication number
CA2904022C
CA2904022C CA2904022A CA2904022A CA2904022C CA 2904022 C CA2904022 C CA 2904022C CA 2904022 A CA2904022 A CA 2904022A CA 2904022 A CA2904022 A CA 2904022A CA 2904022 C CA2904022 C CA 2904022C
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CA
Canada
Prior art keywords
variant
glucoamylase
substitutions
polypeptide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2904022A
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English (en)
French (fr)
Other versions
CA2904022A1 (en
Inventor
Noriko Tsutsumi
Keiichi Ayabe
Siik KISHISHITA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
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Novozymes AS
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Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Publication of CA2904022A1 publication Critical patent/CA2904022A1/en
Application granted granted Critical
Publication of CA2904022C publication Critical patent/CA2904022C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2904022A 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same Active CA2904022C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13165995 2013-04-30
EP13165995.5 2013-04-30
PCT/EP2014/058692 WO2014177546A2 (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
CA2904022A1 CA2904022A1 (en) 2014-11-06
CA2904022C true CA2904022C (en) 2022-04-19

Family

ID=48190353

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2904022A Active CA2904022C (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Country Status (9)

Country Link
US (1) US10550374B2 (https=)
EP (2) EP3372680B1 (https=)
CN (2) CN105164253B (https=)
BR (1) BR112015026972B1 (https=)
CA (1) CA2904022C (https=)
DK (2) DK2992092T3 (https=)
ES (2) ES2674701T3 (https=)
MX (2) MX368655B (https=)
WO (1) WO2014177546A2 (https=)

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WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
AU2016225049B2 (en) 2015-02-27 2022-03-31 Microbiogen Pty. Ltd. Processes of producing ethanol using a fermenting organism
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US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
BR112019021223A2 (pt) * 2017-04-11 2020-04-28 Novozymes A/S variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
CN110730824A (zh) 2017-06-02 2020-01-24 诺维信公司 用于乙醇生产的改善的酵母
US11584783B2 (en) 2017-06-28 2023-02-21 Novozymes A/S Processes for producing a fermentation product
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EP3746545A1 (en) 2018-01-29 2020-12-09 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
AU2019222480B9 (en) 2018-02-15 2024-11-28 Microbiogen Pty. Ltd. Improved yeast for ethanol production
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WO2021021458A1 (en) 2019-07-26 2021-02-04 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
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CA3158982A1 (en) 2019-12-10 2021-06-17 Monica TASSONE Microorganism for improved pentose fermentation
AU2022288057A1 (en) 2021-06-07 2023-12-21 Novozymes A/S Engineered microorganism for improved ethanol fermentation
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
JP2025541374A (ja) 2022-12-19 2025-12-18 ノボザイムス アクティーゼルスカブ アラビノフラノシダーゼ及びキシラナーゼを含む組成物、並びにヘミセルロース系繊維の可溶化を増加させるためのその使用
EP4638724A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
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Also Published As

Publication number Publication date
EP3372680A1 (en) 2018-09-12
MX2019005158A (es) 2019-08-05
DK3372680T3 (da) 2021-01-11
CA2904022A1 (en) 2014-11-06
EP3372680B1 (en) 2020-11-11
CN105164253A (zh) 2015-12-16
BR112015026972B1 (pt) 2023-05-02
WO2014177546A2 (en) 2014-11-06
EP2992092A2 (en) 2016-03-09
EP2992092B1 (en) 2018-04-04
ES2674701T3 (es) 2018-07-03
MX368655B (es) 2019-10-10
US10550374B2 (en) 2020-02-04
CN121380029A (zh) 2026-01-23
MX2015014537A (es) 2016-02-05
DK2992092T3 (en) 2018-06-25
CN105164253B (zh) 2025-11-14
ES2835313T3 (es) 2021-06-22
BR112015026972A2 (https=) 2017-09-05
WO2014177546A3 (en) 2014-12-24
US20160152960A1 (en) 2016-06-02

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