BR112015026972B1 - Variantes de glucoamilase, composição, uso de um polipeptídeo, processo de produção de um produto de fermentação e processo de produção de um produto de xarope a partir de material contendo amido - Google Patents

Variantes de glucoamilase, composição, uso de um polipeptídeo, processo de produção de um produto de fermentação e processo de produção de um produto de xarope a partir de material contendo amido Download PDF

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Publication number
BR112015026972B1
BR112015026972B1 BR112015026972-9A BR112015026972A BR112015026972B1 BR 112015026972 B1 BR112015026972 B1 BR 112015026972B1 BR 112015026972 A BR112015026972 A BR 112015026972A BR 112015026972 B1 BR112015026972 B1 BR 112015026972B1
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BR
Brazil
Prior art keywords
variant
glucoamylase
polypeptide
positions
alpha
Prior art date
Application number
BR112015026972-9A
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English (en)
Portuguese (pt)
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BR112015026972A2 (https=
Inventor
Siik Kishishita
Noriko Tsutsumi
Keiichi Ayabe
Original Assignee
Novozymes A/S
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Application filed by Novozymes A/S filed Critical Novozymes A/S
Publication of BR112015026972A2 publication Critical patent/BR112015026972A2/pt
Publication of BR112015026972B1 publication Critical patent/BR112015026972B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BR112015026972-9A 2013-04-30 2014-04-29 Variantes de glucoamilase, composição, uso de um polipeptídeo, processo de produção de um produto de fermentação e processo de produção de um produto de xarope a partir de material contendo amido BR112015026972B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13165995 2013-04-30
EP13165995.5 2013-04-30
PCT/EP2014/058692 WO2014177546A2 (en) 2013-04-30 2014-04-29 Glucoamylase variants and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
BR112015026972A2 BR112015026972A2 (https=) 2017-09-05
BR112015026972B1 true BR112015026972B1 (pt) 2023-05-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015026972-9A BR112015026972B1 (pt) 2013-04-30 2014-04-29 Variantes de glucoamilase, composição, uso de um polipeptídeo, processo de produção de um produto de fermentação e processo de produção de um produto de xarope a partir de material contendo amido

Country Status (9)

Country Link
US (1) US10550374B2 (https=)
EP (2) EP3372680B1 (https=)
CN (2) CN105164253B (https=)
BR (1) BR112015026972B1 (https=)
CA (1) CA2904022C (https=)
DK (2) DK3372680T3 (https=)
ES (2) ES2674701T3 (https=)
MX (2) MX368655B (https=)
WO (1) WO2014177546A2 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014039773A1 (en) 2012-09-07 2014-03-13 Novozymes A/S Glucoamylase variants and polynucleotides encoding same and uses thereof
EP3712240B1 (en) 2014-02-07 2023-09-27 Novozymes A/S Compositions for producing glucose syrups
WO2016062875A2 (en) 2014-10-23 2016-04-28 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10308963B2 (en) 2015-02-27 2019-06-04 Novozymes A/S Processes of producing ethanol using a fermenting organism
US10676727B2 (en) 2015-06-18 2020-06-09 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US10494685B2 (en) 2015-10-14 2019-12-03 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US9598680B1 (en) * 2016-08-05 2017-03-21 Fornia Biosolutions, Inc. G16 glucoamylase compositions and methods
US10889836B2 (en) 2016-11-23 2021-01-12 Novozymes A/S Yeast for ethanol production
CN110651048A (zh) * 2017-04-11 2020-01-03 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
WO2018222990A1 (en) 2017-06-02 2018-12-06 Novozymes A/S Improved yeast for ethanol production
ES2908190T3 (es) 2017-06-28 2022-04-28 Novozymes As Polipéptidos con actividad de trehalasa y polinucleótidos que los codifican
CA3070281A1 (en) 2017-08-08 2019-02-14 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
CA3089135A1 (en) 2018-01-29 2019-08-01 Novozymes A/S Microorganisms with improved nitrogen utilization for ethanol production
US11473109B2 (en) 2018-02-15 2022-10-18 Novozymes A/S Yeast for ethanol production
CA3098718A1 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
BR112021001282A2 (pt) 2018-07-25 2021-04-27 Novozymes A/S levedura expressando enzimas para produção de etanol
CA3114783A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
EP4004029A1 (en) 2019-07-26 2022-06-01 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
MX2021015818A (es) 2019-08-06 2022-02-03 Novozymes As Proteinas de fusion para mejorar la expresion de enzimas.
AU2020402990A1 (en) 2019-12-10 2022-06-09 Novozymes A/S Microorganism for improved pentose fermentation
BR112023025624A2 (pt) 2021-06-07 2024-02-27 Novozymes As Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2026008449A2 (en) 2024-07-04 2026-01-08 Novozymes A/S A process for producing a fermentation product and a concentrated protein co-product

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0383779B2 (en) 1987-09-04 2000-05-31 Novo Nordisk A/S PROCESS FOR THE PRODUCTION OF PROTEIN PRODUCTS IN $i(ASPERGILLUS) AND PROMOTERS FOR USE IN $i(ASPERGILLUS)
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1995033836A1 (en) 1994-06-03 1995-12-14 Novo Nordisk Biotech, Inc. Phosphonyldipeptides useful in the treatment of cardiovascular diseases
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
DK2199378T3 (da) 1995-02-03 2012-10-29 Novozymes As alfa-amylase mutanter
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
EP0904360B1 (en) 1996-04-30 2013-07-31 Novozymes A/S alpha-AMYLASE MUTANTS
AR017331A1 (es) 1997-10-13 2001-09-05 Novozymes As Polipeptidos mutantes de alfa-amilasas, aditivo para detergentes y composiciones detergentes que los comprenden.
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
RU2258739C2 (ru) 1998-02-27 2005-08-20 Новозимс А/С ВАРИАНТ МАЛЬТОГЕННОЙ α-АМИЛАЗЫ
DK1124949T3 (da) 1998-10-26 2006-11-06 Novozymes As Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
DK2290060T3 (en) 1999-03-30 2017-03-06 Novozymes As The alpha-amylase variants
EP1370648A2 (en) 2000-08-01 2003-12-17 Novozymes A/S Alpha-amylase mutants with altered properties
EP1421187B1 (en) 2001-07-27 2007-10-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Systems for in vivo site-directed mutagenesis using oligonucleotides
CN102277341B (zh) 2003-03-10 2015-07-22 诺维信公司 酒精产品生产方法
ES2425351T3 (es) 2003-06-25 2013-10-14 Novozymes A/S Polipéptidos que tienen actividad de alfa-amilasa y polinucleótidos que codifican los mismos
WO2005003311A2 (en) 2003-06-25 2005-01-13 Novozymes A/S Enzymes for starch processing
EP2365068B1 (en) 2004-12-22 2017-03-01 Novozymes A/S Enzymes for starch processing
WO2007144424A2 (en) 2006-06-15 2007-12-21 Novozymes A/S Processes for production of a starch hydrolysate
WO2011066560A1 (en) 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
MX2012006096A (es) * 2009-11-30 2012-07-03 Novozymes North America Inc Polipeptidos que tienen actividad glucoamilasa y polinucleotidos que codifican para los mismos.
ES2534067T3 (es) 2009-12-01 2015-04-17 Novozymes A/S Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi

Also Published As

Publication number Publication date
EP3372680A1 (en) 2018-09-12
CA2904022C (en) 2022-04-19
WO2014177546A2 (en) 2014-11-06
MX2015014537A (es) 2016-02-05
BR112015026972A2 (https=) 2017-09-05
CA2904022A1 (en) 2014-11-06
ES2835313T3 (es) 2021-06-22
EP2992092A2 (en) 2016-03-09
DK2992092T3 (en) 2018-06-25
EP2992092B1 (en) 2018-04-04
US10550374B2 (en) 2020-02-04
CN105164253A (zh) 2015-12-16
US20160152960A1 (en) 2016-06-02
DK3372680T3 (da) 2021-01-11
ES2674701T3 (es) 2018-07-03
MX368655B (es) 2019-10-10
MX2019005158A (es) 2019-08-05
CN105164253B (zh) 2025-11-14
CN121380029A (zh) 2026-01-23
WO2014177546A3 (en) 2014-12-24
EP3372680B1 (en) 2020-11-11

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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