CA2901974C - Method for producing ethanol using recombinant yeast - Google Patents

Method for producing ethanol using recombinant yeast Download PDF

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Publication number
CA2901974C
CA2901974C CA2901974A CA2901974A CA2901974C CA 2901974 C CA2901974 C CA 2901974C CA 2901974 A CA2901974 A CA 2901974A CA 2901974 A CA2901974 A CA 2901974A CA 2901974 C CA2901974 C CA 2901974C
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Canada
Prior art keywords
gene
amino acid
protein
xylose
seq
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CA2901974A
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English (en)
French (fr)
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CA2901974A1 (en
Inventor
Toru Onishi
Nobuki Tada
Noriko Yasutani
Satoshi Katahira
Nobuhiro Ishida
Risa NAGURA
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Toyota Motor Corp
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Publication of CA2901974A1 publication Critical patent/CA2901974A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2901974A 2013-02-27 2014-02-27 Method for producing ethanol using recombinant yeast Active CA2901974C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2013037501 2013-02-27
JP2013-037501 2013-02-27
JP2014036652A JP6087854B2 (ja) 2013-02-27 2014-02-27 組換え酵母を用いたエタノールの製造方法
PCT/JP2014/054915 WO2014133092A1 (ja) 2013-02-27 2014-02-27 組換え酵母を用いたエタノールの製造方法
JP2014-036652 2014-02-27

Publications (2)

Publication Number Publication Date
CA2901974A1 CA2901974A1 (en) 2014-09-04
CA2901974C true CA2901974C (en) 2020-01-14

Family

ID=51428351

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2901974A Active CA2901974C (en) 2013-02-27 2014-02-27 Method for producing ethanol using recombinant yeast

Country Status (6)

Country Link
US (1) US20160002674A1 (https=)
JP (1) JP6087854B2 (https=)
CN (2) CN114774477B (https=)
BR (1) BR112015019777B1 (https=)
CA (1) CA2901974C (https=)
WO (1) WO2014133092A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9890398B2 (en) 2011-10-24 2018-02-13 Toyota Jidosha Kabushiki Kaisha Method for producing ethanol using recombinant yeast
JP6027559B2 (ja) * 2013-03-28 2016-11-16 株式会社豊田中央研究所 キシロースイソメラーゼ活性を有するタンパク質及びその利用
AU2015331353B2 (en) * 2014-10-15 2021-11-25 Jx Nippon Oil & Energy Corporation Yeast capable of producing ethanol from xylose
JP6303984B2 (ja) 2014-10-31 2018-04-04 トヨタ自動車株式会社 連続培養によるエタノールの製造方法及び連続培養装置
JP6697250B2 (ja) * 2015-12-02 2020-05-20 株式会社豊田中央研究所 ゲノム再編方法及びその利用
JP6447583B2 (ja) * 2016-06-24 2019-01-09 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
JP6097869B1 (ja) * 2016-08-01 2017-03-15 新日鉄住金エンジニアリング株式会社 エタノールの製造方法
JP6879111B2 (ja) * 2017-08-02 2021-06-02 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
US11739338B2 (en) 2017-09-19 2023-08-29 Lallemand Hungary Liquidity Management Llc Acetate toxicity tolerance in recombinant microbial host cells
JP2019068788A (ja) * 2017-10-11 2019-05-09 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
JP7078887B2 (ja) 2017-11-06 2022-06-01 トヨタ自動車株式会社 エタノール発酵によるエタノール生産性の向上に関与する変異遺伝子及びこれを用いたエタノールの製造方法
JP7298674B2 (ja) * 2017-11-06 2023-06-27 トヨタ自動車株式会社 エタノール発酵によるエタノール生産性の向上に関与する変異遺伝子及びこれを用いたエタノールの製造方法
JP7298673B2 (ja) * 2017-11-06 2023-06-27 トヨタ自動車株式会社 エタノール発酵によるエタノール生産性の向上に関与する変異遺伝子及びこれを用いたエタノールの製造方法
KR20200135469A (ko) * 2018-03-27 2020-12-02 바스프 에스이 자일로스 대사 효모
JP6745414B2 (ja) 2018-03-27 2020-08-26 積水化学工業株式会社 エタノールの製造方法及びエタノール組成物
JP2020025493A (ja) * 2018-08-10 2020-02-20 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
CN113046252B (zh) * 2021-03-23 2022-07-05 江南大学 一株乙醛脱氢酶高产菌株的分离与鉴定
CN114107368B (zh) * 2021-11-29 2023-05-26 重庆大学 表达反式菊酸的联合表达载体及其在调控番茄vi型腺体腺毛合成反式菊酸中的应用
CN117384871B (zh) * 2023-12-13 2024-03-01 北京科为博生物科技有限公司 一种热稳定性提高的乙醛脱氢酶及其基因和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001520A1 (en) * 1987-08-11 1989-02-23 Cetus Corporation Procaryotic xylose isomerase muteins and method to increase protein stability
JP5091523B2 (ja) * 2007-03-30 2012-12-05 三井造船株式会社 アルコール生産方法
WO2009013159A2 (en) * 2007-07-23 2009-01-29 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
JP5608999B2 (ja) * 2009-04-08 2014-10-22 株式会社豊田中央研究所 キシロースを利用して有用物質を生産する方法
US20120184007A1 (en) * 2009-07-09 2012-07-19 Stephen Picataggio Engineered microorganisms with enhanced fermentation activity
EP2277989A1 (en) * 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
JP5321320B2 (ja) * 2009-07-27 2013-10-23 株式会社豊田中央研究所 発酵能力が向上された酵母及びその利用
CN102791858B (zh) * 2009-12-22 2014-11-26 株式会社丰田中央研究所 木糖异构酶及其利用
US9605269B2 (en) * 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
EP2633030A1 (en) * 2010-10-29 2013-09-04 Novozymes A/S Recombinant n-propanol and isopropanol production
WO2012067510A1 (en) * 2010-11-18 2012-05-24 C5 Yeast Company B.V. Yeast strains engineered to produce ethanol from glycerol
WO2014074895A2 (en) * 2012-11-09 2014-05-15 Mascoma Corporation Method for acetate consumption during ethanolic fermentation of cellulosic feedstocks

Also Published As

Publication number Publication date
CN114774477A (zh) 2022-07-22
JP6087854B2 (ja) 2017-03-01
CN114774477B (zh) 2024-04-09
JP2014193152A (ja) 2014-10-09
CN105073990A (zh) 2015-11-18
WO2014133092A1 (ja) 2014-09-04
US20160002674A1 (en) 2016-01-07
BR112015019777A2 (https=) 2017-08-22
BR112015019777B1 (pt) 2022-12-06
CA2901974A1 (en) 2014-09-04

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