CA2891028C - In vitro capture and analysis of circulating tumor cells - Google Patents
In vitro capture and analysis of circulating tumor cells Download PDFInfo
- Publication number
- CA2891028C CA2891028C CA2891028A CA2891028A CA2891028C CA 2891028 C CA2891028 C CA 2891028C CA 2891028 A CA2891028 A CA 2891028A CA 2891028 A CA2891028 A CA 2891028A CA 2891028 C CA2891028 C CA 2891028C
- Authority
- CA
- Canada
- Prior art keywords
- seprase
- epcam
- cells
- tumor
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 109
- 238000000338 in vitro Methods 0.000 title abstract description 8
- 238000004458 analytical method Methods 0.000 title description 8
- 102100023832 Prolyl endopeptidase FAP Human genes 0.000 claims abstract description 105
- 108010072257 fibroblast activation protein alpha Proteins 0.000 claims abstract description 94
- 238000000034 method Methods 0.000 claims abstract description 56
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 40
- 206010061289 metastatic neoplasm Diseases 0.000 claims abstract description 36
- 210000004027 cell Anatomy 0.000 claims description 104
- 108010066687 Epithelial Cell Adhesion Molecule Proteins 0.000 claims description 76
- 102000018651 Epithelial Cell Adhesion Molecule Human genes 0.000 claims description 76
- 206010028980 Neoplasm Diseases 0.000 claims description 75
- 201000011510 cancer Diseases 0.000 claims description 31
- 210000004369 blood Anatomy 0.000 claims description 27
- 239000008280 blood Substances 0.000 claims description 27
- -1 CK18 Proteins 0.000 claims description 20
- 210000004881 tumor cell Anatomy 0.000 claims description 15
- 239000000090 biomarker Substances 0.000 claims description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 14
- 101000998011 Homo sapiens Keratin, type I cytoskeletal 19 Proteins 0.000 claims description 11
- 102100033420 Keratin, type I cytoskeletal 19 Human genes 0.000 claims description 11
- 238000004113 cell culture Methods 0.000 claims description 11
- 239000003446 ligand Substances 0.000 claims description 11
- 102100023126 Cell surface glycoprotein MUC18 Human genes 0.000 claims description 10
- 101000623903 Homo sapiens Cell surface glycoprotein MUC18 Proteins 0.000 claims description 10
- 101000884271 Homo sapiens Signal transducer CD24 Proteins 0.000 claims description 8
- 102100038081 Signal transducer CD24 Human genes 0.000 claims description 8
- 102100031102 C-C motif chemokine 4 Human genes 0.000 claims description 7
- 102100031650 C-X-C chemokine receptor type 4 Human genes 0.000 claims description 7
- 102100032912 CD44 antigen Human genes 0.000 claims description 7
- 101000922348 Homo sapiens C-X-C chemokine receptor type 4 Proteins 0.000 claims description 7
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims description 7
- 101000584612 Homo sapiens GTPase KRas Proteins 0.000 claims description 7
- 101000610551 Homo sapiens Prominin-1 Proteins 0.000 claims description 7
- 101000803403 Homo sapiens Vimentin Proteins 0.000 claims description 7
- 101000777470 Mus musculus C-C motif chemokine 4 Proteins 0.000 claims description 7
- 102100040120 Prominin-1 Human genes 0.000 claims description 7
- 102100035071 Vimentin Human genes 0.000 claims description 7
- 239000003112 inhibitor Substances 0.000 claims description 7
- 108091008104 nucleic acid aptamers Proteins 0.000 claims description 7
- 102100030708 GTPase KRas Human genes 0.000 claims description 6
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 claims description 6
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 claims description 6
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims description 6
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims description 6
- 102000006280 Twist-Related Protein 1 Human genes 0.000 claims description 6
- 108010083162 Twist-Related Protein 1 Proteins 0.000 claims description 6
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 claims description 5
- 108090001008 Avidin Proteins 0.000 claims description 5
- 108091012583 BCL2 Proteins 0.000 claims description 5
- 102100021663 Baculoviral IAP repeat-containing protein 5 Human genes 0.000 claims description 5
- 102000000905 Cadherin Human genes 0.000 claims description 5
- 108050007957 Cadherin Proteins 0.000 claims description 5
- 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 claims description 5
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 claims description 5
- 102100031983 Ephrin type-B receptor 4 Human genes 0.000 claims description 5
- 101000914324 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 5 Proteins 0.000 claims description 5
- 101000914321 Homo sapiens Carcinoembryonic antigen-related cell adhesion molecule 7 Proteins 0.000 claims description 5
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 claims description 5
- 101001133056 Homo sapiens Mucin-1 Proteins 0.000 claims description 5
- 101000617725 Homo sapiens Pregnancy-specific beta-1-glycoprotein 2 Proteins 0.000 claims description 5
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 claims description 5
- 102100034256 Mucin-1 Human genes 0.000 claims description 5
- 108050000637 N-cadherin Proteins 0.000 claims description 5
- 102000014160 PTEN Phosphohydrolase Human genes 0.000 claims description 5
- 108010011536 PTEN Phosphohydrolase Proteins 0.000 claims description 5
- 108010079855 Peptide Aptamers Proteins 0.000 claims description 5
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 claims description 5
- 108010002687 Survivin Proteins 0.000 claims description 5
- 210000001124 body fluid Anatomy 0.000 claims description 5
- 239000010839 body fluid Substances 0.000 claims description 5
- 239000006285 cell suspension Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 claims description 5
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 claims description 5
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 claims description 5
- MXVJJCFZNPIZDA-VWMHFEHESA-N P(OC1=CC=CC=C1)(OC1=CC=CC=C1)=O.N1[C@H](C(=O)O)CCC1 Chemical compound P(OC1=CC=CC=C1)(OC1=CC=CC=C1)=O.N1[C@H](C(=O)O)CCC1 MXVJJCFZNPIZDA-VWMHFEHESA-N 0.000 claims description 4
- 102100029986 Receptor tyrosine-protein kinase erbB-3 Human genes 0.000 claims description 4
- 101710100969 Receptor tyrosine-protein kinase erbB-3 Proteins 0.000 claims description 4
- UUOZISWTWURDGU-SSDOTTSWSA-N [(2S)-1-(2-acetamidoacetyl)pyrrolidin-2-yl]boronic acid Chemical compound CC(=O)NCC(=O)N1CCC[C@@H]1B(O)O UUOZISWTWURDGU-SSDOTTSWSA-N 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 239000011230 binding agent Substances 0.000 claims description 3
- 230000036963 noncompetitive effect Effects 0.000 claims description 3
- 230000002860 competitive effect Effects 0.000 claims description 2
- 230000007515 enzymatic degradation Effects 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 230000001394 metastastic effect Effects 0.000 abstract description 28
- 239000000203 mixture Substances 0.000 abstract description 4
- 238000001727 in vivo Methods 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 description 25
- 239000000523 sample Substances 0.000 description 24
- 229920000371 poly(diallyldimethylammonium chloride) polymer Polymers 0.000 description 16
- 108090000623 proteins and genes Proteins 0.000 description 15
- 101710088083 Glomulin Proteins 0.000 description 13
- 238000010186 staining Methods 0.000 description 13
- 206010006187 Breast cancer Diseases 0.000 description 11
- 208000026310 Breast neoplasm Diseases 0.000 description 11
- 230000007705 epithelial mesenchymal transition Effects 0.000 description 11
- 239000003550 marker Substances 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 9
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 8
- 206010027476 Metastases Diseases 0.000 description 8
- 201000001441 melanoma Diseases 0.000 description 8
- 230000009401 metastasis Effects 0.000 description 8
- 238000003801 milling Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 238000001514 detection method Methods 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 206010009944 Colon cancer Diseases 0.000 description 6
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 6
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 6
- 230000027455 binding Effects 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 5
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 5
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 5
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 5
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 5
- 210000002744 extracellular matrix Anatomy 0.000 description 5
- 210000002950 fibroblast Anatomy 0.000 description 5
- 201000002528 pancreatic cancer Diseases 0.000 description 5
- 230000008685 targeting Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 206010027480 Metastatic malignant melanoma Diseases 0.000 description 4
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 210000003169 central nervous system Anatomy 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 208000014829 head and neck neoplasm Diseases 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 4
- 208000021039 metastatic melanoma Diseases 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 208000002154 non-small cell lung carcinoma Diseases 0.000 description 4
- 208000008443 pancreatic carcinoma Diseases 0.000 description 4
- 238000005498 polishing Methods 0.000 description 4
- 229920000307 polymer substrate Polymers 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000004393 prognosis Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000009870 specific binding Effects 0.000 description 4
- 210000000130 stem cell Anatomy 0.000 description 4
- 208000029729 tumor suppressor gene on chromosome 11 Diseases 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- SXGZJKUKBWWHRA-UHFFFAOYSA-N 2-(N-morpholiniumyl)ethanesulfonate Chemical compound [O-]S(=O)(=O)CC[NH+]1CCOCC1 SXGZJKUKBWWHRA-UHFFFAOYSA-N 0.000 description 3
- 206010052358 Colorectal cancer metastatic Diseases 0.000 description 3
- 229920000089 Cyclic olefin copolymer Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 206010017758 gastric cancer Diseases 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000001356 surgical procedure Methods 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108091023037 Aptamer Proteins 0.000 description 2
- 206010003571 Astrocytoma Diseases 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 2
- 206010005949 Bone cancer Diseases 0.000 description 2
- 208000018084 Bone neoplasm Diseases 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- 206010008342 Cervix carcinoma Diseases 0.000 description 2
- 108010016626 Dipeptides Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 206010014733 Endometrial cancer Diseases 0.000 description 2
- 206010014759 Endometrial neoplasm Diseases 0.000 description 2
- 108700039887 Essential Genes Proteins 0.000 description 2
- 208000032612 Glial tumor Diseases 0.000 description 2
- 201000010915 Glioblastoma multiforme Diseases 0.000 description 2
- 206010018338 Glioma Diseases 0.000 description 2
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 208000008839 Kidney Neoplasms Diseases 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 206010033128 Ovarian cancer Diseases 0.000 description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 description 2
- 108010004729 Phycoerythrin Proteins 0.000 description 2
- 206010060862 Prostate cancer Diseases 0.000 description 2
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 206010038389 Renal cancer Diseases 0.000 description 2
- 208000006265 Renal cell carcinoma Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 102000012479 Serine Proteases Human genes 0.000 description 2
- 108010022999 Serine Proteases Proteins 0.000 description 2
- 208000005718 Stomach Neoplasms Diseases 0.000 description 2
- 208000024770 Thyroid neoplasm Diseases 0.000 description 2
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 2
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 2
- 208000002495 Uterine Neoplasms Diseases 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000010951 brass Substances 0.000 description 2
- 108010043595 captavidin Proteins 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 201000010881 cervical cancer Diseases 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910003460 diamond Inorganic materials 0.000 description 2
- 239000010432 diamond Substances 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 208000005017 glioblastoma Diseases 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 2
- 231100000844 hepatocellular carcinoma Toxicity 0.000 description 2
- 238000000126 in silico method Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 201000010982 kidney cancer Diseases 0.000 description 2
- 238000012933 kinetic analysis Methods 0.000 description 2
- 201000007270 liver cancer Diseases 0.000 description 2
- 208000014018 liver neoplasm Diseases 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 208000026037 malignant tumor of neck Diseases 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 238000010837 poor prognosis Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 230000004850 protein–protein interaction Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- 201000011549 stomach cancer Diseases 0.000 description 2
- 238000012916 structural analysis Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 201000002510 thyroid cancer Diseases 0.000 description 2
- 239000000107 tumor biomarker Substances 0.000 description 2
- 201000005112 urinary bladder cancer Diseases 0.000 description 2
- 206010046766 uterine cancer Diseases 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 1
- 101150029062 15 gene Proteins 0.000 description 1
- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 description 1
- 208000035657 Abasia Diseases 0.000 description 1
- 206010069754 Acquired gene mutation Diseases 0.000 description 1
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 description 1
- 108060000903 Beta-catenin Proteins 0.000 description 1
- 102000015735 Beta-catenin Human genes 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 108010058905 CD44v6 antigen Proteins 0.000 description 1
- 102000016289 Cell Adhesion Molecules Human genes 0.000 description 1
- 108010067225 Cell Adhesion Molecules Proteins 0.000 description 1
- 102100026735 Coagulation factor VIII Human genes 0.000 description 1
- 239000004713 Cyclic olefin copolymer Substances 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 102100038595 Estrogen receptor Human genes 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- 108091092584 GDNA Proteins 0.000 description 1
- 102000013382 Gelatinases Human genes 0.000 description 1
- 108010026132 Gelatinases Proteins 0.000 description 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108010067902 Peptide Library Proteins 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 208000003721 Triple Negative Breast Neoplasms Diseases 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 208000009956 adenocarcinoma Diseases 0.000 description 1
- 238000011226 adjuvant chemotherapy Methods 0.000 description 1
- 238000003314 affinity selection Methods 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 208000012999 benign epithelial neoplasm Diseases 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 201000008274 breast adenocarcinoma Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 101150082482 ctc gene Proteins 0.000 description 1
- 208000035250 cutaneous malignant susceptibility to 1 melanoma Diseases 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 210000005220 cytoplasmic tail Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004836 empirical method Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 230000004049 epigenetic modification Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 108010038795 estrogen receptors Proteins 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 208000010749 gastric carcinoma Diseases 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009477 glass transition Effects 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 201000005787 hematologic cancer Diseases 0.000 description 1
- 208000024200 hematopoietic and lymphoid system neoplasm Diseases 0.000 description 1
- 239000000710 homodimer Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- ONCZDRURRATYFI-QTCHDTBASA-N methyl (2z)-2-methoxyimino-2-[2-[[(e)-1-[3-(trifluoromethyl)phenyl]ethylideneamino]oxymethyl]phenyl]acetate Chemical compound CO\N=C(/C(=O)OC)C1=CC=CC=C1CO\N=C(/C)C1=CC=CC(C(F)(F)F)=C1 ONCZDRURRATYFI-QTCHDTBASA-N 0.000 description 1
- 238000004226 microchip electrophoresis Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000002061 nanopillar Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 108010087904 neutravidin Proteins 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 208000011581 secondary neoplasm Diseases 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 230000037439 somatic mutation Effects 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- JGVWCANSWKRBCS-UHFFFAOYSA-N tetramethylrhodamine thiocyanate Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(SC#N)C=C1C(O)=O JGVWCANSWKRBCS-UHFFFAOYSA-N 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 229920001169 thermoplastic Polymers 0.000 description 1
- 239000004416 thermosoftening plastic Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 208000022679 triple-negative breast carcinoma Diseases 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 238000010396 two-hybrid screening Methods 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96433—Serine endopeptidases (3.4.21)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96466—Cysteine endopeptidases (3.4.22)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hospice & Palliative Care (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261724753P | 2012-11-09 | 2012-11-09 | |
| US61/724,753 | 2012-11-09 | ||
| PCT/EP2013/073381 WO2014072465A1 (en) | 2012-11-09 | 2013-11-08 | In vitro capture and analysis of circulating tumor cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2891028A1 CA2891028A1 (en) | 2014-05-15 |
| CA2891028C true CA2891028C (en) | 2017-02-07 |
Family
ID=49582733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2891028A Active CA2891028C (en) | 2012-11-09 | 2013-11-08 | In vitro capture and analysis of circulating tumor cells |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US9250242B2 (enExample) |
| EP (1) | EP2919910B1 (enExample) |
| JP (1) | JP6404824B2 (enExample) |
| CN (1) | CN104781007B (enExample) |
| CA (1) | CA2891028C (enExample) |
| ES (1) | ES2660438T3 (enExample) |
| WO (1) | WO2014072465A1 (enExample) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10155988B2 (en) * | 2010-04-07 | 2018-12-18 | Board Of Regents, The University Of Texas System | Methods of detecting tumor cells |
| TWI539158B (zh) * | 2010-06-08 | 2016-06-21 | 維里德克斯有限責任公司 | 使用血液中之循環黑色素瘤細胞預測黑色素瘤病患之臨床結果的方法。 |
| WO2012170560A2 (en) | 2011-06-06 | 2012-12-13 | Cornell University | Microfluidic device for extracting, isolating, and analyzing dna from cells |
| WO2013049636A1 (en) | 2011-09-30 | 2013-04-04 | The Regents Of The University Of Michigan | System for detecting rare cells |
| US10130946B2 (en) | 2011-09-30 | 2018-11-20 | The Regents Of The University Of Michigan | System for detecting rare cells |
| US10073024B2 (en) | 2012-10-29 | 2018-09-11 | The Regents Of The University Of Michigan | Microfluidic device and method for detecting rare cells |
| WO2014072465A1 (en) | 2012-11-09 | 2014-05-15 | Roche Diagnostics Gmbh | In vitro capture and analysis of circulating tumor cells |
| EP2799872B1 (en) * | 2013-05-01 | 2017-10-18 | F. Hoffmann-La Roche AG | Selective release of sub-group of biological units |
| US9709556B2 (en) | 2013-12-11 | 2017-07-18 | Analiza, Inc. | Devices and methods for determining and/or isolating circulating cancer cells |
| US20150160246A1 (en) | 2013-12-11 | 2015-06-11 | Analiza, Inc. | Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells |
| ES2970741T3 (es) * | 2014-08-25 | 2024-05-30 | Creatv Microtech Inc | Utilización de biomarcadores celulares circulantes en la sangre para la detección y el diagnóstico de enfermedades y métodos para aislarlos |
| US10829804B2 (en) | 2015-03-23 | 2020-11-10 | The University Of North Carolina At Chapel Hill | Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or DNA methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions |
| US10393726B2 (en) | 2015-03-23 | 2019-08-27 | The University Of North Carolina At Chapel Hill | Universal molecular processor for precision medicine |
| US10317406B2 (en) | 2015-04-06 | 2019-06-11 | The Regents Of The University Of Michigan | System for detecting rare cells |
| CN105259096B (zh) * | 2015-10-19 | 2018-01-12 | 武汉顺可达生物科技有限公司 | 磷酸锌纳米基底及其制备方法和在循环肿瘤细胞捕获与释放中的应用 |
| CN106868098A (zh) * | 2015-12-10 | 2017-06-20 | 益善生物技术股份有限公司 | 循环肿瘤细胞分型鉴定试剂盒 |
| WO2017156341A1 (en) | 2016-03-09 | 2017-09-14 | Beijing Percans Oncology Co. Ltd. | Tumor cell suspension cultures and related methods |
| WO2017188346A1 (ja) * | 2016-04-27 | 2017-11-02 | 国立大学法人 東京大学 | マイクロファイバーを用いた血中循環細胞の捕捉及び回収用材料及び当該材料を用いる方法 |
| WO2017199651A1 (ja) * | 2016-05-18 | 2017-11-23 | ソニー株式会社 | 生体物質分析装置、生体物質分析システム、生体物質選別方法、生体物質分析用プログラム及び細胞培養容器 |
| US11602747B2 (en) | 2016-05-22 | 2023-03-14 | Cornell University | Multifunctional microfluidic device for capturing target cells and analyzing genomic DNA isolated from the target cells while under flow conditions |
| CN107475354A (zh) * | 2016-06-08 | 2017-12-15 | 益善生物技术股份有限公司 | 前列腺癌组织溯源ctc分型鉴定试剂盒 |
| CN107475355A (zh) * | 2016-06-08 | 2017-12-15 | 益善生物技术股份有限公司 | 肺癌早期筛查试剂盒 |
| CN106366197B (zh) * | 2016-08-31 | 2021-03-30 | 上海美吉生物医药科技有限公司 | 一种HER2、EGFR、EpCAM、MUC1多重抗体免疫磁珠及其制备方法 |
| CN106434904A (zh) * | 2016-09-07 | 2017-02-22 | 上海交通大学医学院附属仁济医院 | 一种实时监控肝癌复发或转移的CD133、EpCAM和CK19联合检测试剂盒 |
| CN110291401B (zh) | 2016-12-14 | 2023-04-11 | 普渡研究基金会 | 成纤维细胞活化蛋白(fap)-靶向成像和治疗 |
| JPWO2018116465A1 (ja) * | 2016-12-22 | 2019-10-24 | 日立化成株式会社 | Ｈｅｒ２陽性癌細胞の検出方法 |
| CN106866820B (zh) * | 2017-02-17 | 2020-04-03 | 艾比玛特医药科技（上海）有限公司 | 一种用于捕获肿瘤细胞的抗人角蛋白18的单克隆抗体及其应用 |
| CN110573629B (zh) * | 2017-03-30 | 2024-08-13 | 伊利诺伊大学理事会 | 用于诊断早期胰腺癌的方法和试剂盒 |
| CN112088023B (zh) | 2018-01-05 | 2024-05-14 | 帕斯艾克斯公司 | 用于捕获和去除流体中的疾病物质的装置 |
| CN108344615B (zh) * | 2018-01-24 | 2020-10-23 | 杭州安弼晟生物科技有限公司 | 一种循环肿瘤细胞捕获系统及控制方法 |
| WO2019239431A1 (en) * | 2018-06-13 | 2019-12-19 | Tzar Management Technology Private Limited | Method for analysing pluripotent stem cell biomarkers, and implementations thereof |
| EP3870969A4 (en) * | 2018-10-24 | 2022-07-27 | Path Ex, Inc. | PROCESS FOR RECOVERING AND ISOLATION OF DISEASE MATERIAL FROM FLOWING MATERIAL |
| WO2020113173A1 (en) | 2018-11-28 | 2020-06-04 | Cornell University | Multiomic analysis of cell analytes using microfluidic systems |
| WO2020113192A1 (en) | 2018-11-28 | 2020-06-04 | Cornell University | Systems and methods for on-chip analysis of nucleic acids and for multiplexed analysis of cells |
| WO2020139211A1 (en) | 2018-12-28 | 2020-07-02 | Mikro Biyosistemler Elektronik Sanayi Ve Ticaret A.S. | A microfluidic device for selective capture of biological entities |
| CN110082529B (zh) * | 2019-03-11 | 2022-04-19 | 南方医科大学深圳医院 | 一种肿瘤细胞表面标志物及其应用 |
| CN112143732B (zh) * | 2019-06-28 | 2022-03-22 | 中国科学院苏州纳米技术与纳米仿生研究所 | 特异性识别N-cadherin的ssDNA适配体、其筛选方法及应用 |
| US12053569B2 (en) | 2019-09-04 | 2024-08-06 | The Regents Of The University Of Michigan | Indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells |
| US20230173487A1 (en) * | 2020-05-25 | 2023-06-08 | Tokyo Ohka Kogyo Co., Ltd. | Target particle separation method and system |
| JPWO2022009642A1 (enExample) * | 2020-07-06 | 2022-01-13 | ||
| CN112569362B (zh) * | 2020-12-23 | 2022-05-17 | 北京航空航天大学 | 一种通过打散ctc细胞团抑制肿瘤转移的方法 |
| CN112924363A (zh) * | 2021-01-22 | 2021-06-08 | 中国科学院苏州纳米技术与纳米仿生研究所 | 中间型循环肿瘤细胞作为肿瘤诊断和预后标志物及其应用 |
| EP4284911A4 (en) * | 2021-01-27 | 2025-01-08 | BioFluidica, Inc. | High efficiency capture of fetal cells from maternal samples |
| CN112730840B (zh) * | 2021-01-29 | 2023-10-17 | 章毅 | 鉴别cd44和cd24分子表型的方法 |
| CN114874989B (zh) * | 2022-04-11 | 2024-06-25 | 中国科学院苏州纳米技术与纳米仿生研究所 | 一种循环肿瘤细胞捕获方法 |
| WO2024021040A1 (zh) * | 2022-07-29 | 2024-02-01 | 曾繁根 | 细胞辨识方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999041613A1 (en) * | 1998-02-12 | 1999-08-19 | Immunivest | Methods and reagents for the rapid and efficient isolation of circulating cancer cells |
| US7785810B2 (en) * | 2000-09-09 | 2010-08-31 | The Research Foundation Of State University Of New York | Method and compositions for isolating metastatic cancer cells, and use in measuring metastatic potential of a cancer thereof |
| AU2004286307A1 (en) * | 2003-10-31 | 2005-05-12 | Vitatex, Inc. | Blood test prototypes and methods for the detection of circulating tumor and endothelial cells |
| WO2008131048A2 (en) * | 2007-04-16 | 2008-10-30 | Cellpoint Diagnotics, Inc. | Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells |
| US8889361B2 (en) | 2007-09-19 | 2014-11-18 | The Research Foundation For The State University Of New York | Gene expression signatures in enriched tumor cell samples |
| EP2223115B1 (en) | 2007-12-10 | 2011-09-14 | Roche Diagnostics GmbH | Seprase as a marker for cancer |
| US10429376B2 (en) | 2008-05-16 | 2019-10-01 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Microfluidic isolation of tumor cells or other rare cells from whole blood or other liquids |
| GR1006582B (el) | 2008-07-14 | 2009-11-03 | Ελενη Στυλιανου Μωυσιαδου | Προϊοντα καθαρισμου και απολυμανσης με φυσικα αβλαβη υλικα που εχουν βαση την ζαχαρη |
| US20100210989A1 (en) * | 2008-12-23 | 2010-08-19 | Janet Lesley Macpherson | Processing blood |
| EP2409151B1 (en) * | 2009-03-18 | 2018-07-11 | The Regents of The University of California | Device for capturing circulating cells |
| EP2995953B1 (en) | 2009-03-24 | 2017-11-29 | Biocept, Inc. | Devices and methods of cell capture and analysis |
| US20120077246A1 (en) | 2009-04-24 | 2012-03-29 | The Board Of Trustees Of The University Of Illinoi | Methods and Devices for Capturing Circulating Tumor Cells |
| SG10201600803SA (en) | 2011-02-03 | 2016-03-30 | Univ Northeastern | Methods and compositions for highly specific capture and release of biological materials |
| CN202279815U (zh) * | 2011-07-19 | 2012-06-20 | 西门子公司 | 循环肿瘤细胞捕获装置 |
| WO2014072465A1 (en) | 2012-11-09 | 2014-05-15 | Roche Diagnostics Gmbh | In vitro capture and analysis of circulating tumor cells |
-
2013
- 2013-11-08 WO PCT/EP2013/073381 patent/WO2014072465A1/en not_active Ceased
- 2013-11-08 EP EP13789770.8A patent/EP2919910B1/en active Active
- 2013-11-08 CA CA2891028A patent/CA2891028C/en active Active
- 2013-11-08 JP JP2015541154A patent/JP6404824B2/ja active Active
- 2013-11-08 ES ES13789770.8T patent/ES2660438T3/es active Active
- 2013-11-08 US US14/075,143 patent/US9250242B2/en active Active
- 2013-11-08 CN CN201380058487.2A patent/CN104781007B/zh active Active
-
2015
- 2015-12-18 US US14/975,230 patent/US9952212B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104781007B (zh) | 2017-11-17 |
| CN104781007A (zh) | 2015-07-15 |
| US9952212B2 (en) | 2018-04-24 |
| JP6404824B2 (ja) | 2018-10-17 |
| WO2014072465A1 (en) | 2014-05-15 |
| US9250242B2 (en) | 2016-02-02 |
| US20140134646A1 (en) | 2014-05-15 |
| ES2660438T3 (es) | 2018-03-22 |
| EP2919910A1 (en) | 2015-09-23 |
| JP2015537210A (ja) | 2015-12-24 |
| US20160139116A1 (en) | 2016-05-19 |
| CA2891028A1 (en) | 2014-05-15 |
| EP2919910B1 (en) | 2017-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2891028C (en) | In vitro capture and analysis of circulating tumor cells | |
| Labib et al. | Circulating tumor cell profiling for precision oncology | |
| Tayebi et al. | Exosome purification and analysis using a facile microfluidic hydrodynamic trapping device | |
| Ribeiro-Samy et al. | Fast and efficient microfluidic cell filter for isolation of circulating tumor cells from unprocessed whole blood of colorectal cancer patients | |
| Stoecklein et al. | Challenges for CTC-based liquid biopsies: low CTC frequency and diagnostic leukapheresis as a potential solution | |
| JP6771010B2 (ja) | 細胞の捕捉および解析のデバイスおよび方法 | |
| Brown et al. | Characterization of circulating tumor cells as a reflection of the tumor heterogeneity: myth or reality? | |
| Ignatiadis et al. | Circulating tumor cells and circulating tumor DNA: challenges and opportunities on the path to clinical utility | |
| Thege et al. | Microfluidic immunocapture of circulating pancreatic cells using parallel EpCAM and MUC1 capture: characterization, optimization and downstream analysis | |
| Dickey et al. | Oligonucleotide aptamers: A next-generation technology for the capture and detection of circulating tumor cells | |
| El-Heliebi et al. | Are morphological criteria sufficient for the identification of circulating tumor cells in renal cancer? | |
| JP6990713B2 (ja) | 解離した固定組織のサイズに基づいた分離 | |
| CN105980615A (zh) | 寡核苷酸探针及其用途 | |
| WO2014197455A1 (en) | Devices and methods for isolating cells | |
| Chowdhury et al. | Circulating tumor cells in cancer diagnostics and prognostics by single-molecule and single-cell characterization | |
| Su et al. | Antibody-Functional Microsphere-Integrated Filter Chip with Inertial Microflow for Size–Immune-Capturing and Digital Detection of Circulating Tumor Cells | |
| Zhu et al. | Dynamically monitoring the clonal evolution of lung cancer based on the molecular characterization of circulating tumor cells using aptamer cocktail-modified nanosubstrates | |
| Lampignano et al. | Enrichment, isolation and molecular characterization of EpCAM-negative circulating tumor cells | |
| CN110945359B (zh) | 基于雄激素受体的变异体的前列腺癌患者筛查方法 | |
| JP2020512813A (ja) | がんのスクリーニング、診断、治療、及び再発における巨細胞の核酸の特徴付けの使用方法 | |
| WO2018107930A1 (zh) | 外周血循环肿瘤细胞检测体系及其应用 | |
| Irmer et al. | Extracellular Vesicles in Liquid Biopsies as Biomarkers for Solid Tumors. Cancers 2023, 15, 1307 | |
| JP6913977B2 (ja) | 前立腺特異膜抗原ベースの前立腺癌患者スクリーニング方法 | |
| CN111033255B (zh) | 基于axl的癌症患者筛查方法 | |
| Stiefel | Automated isolation of circulating tumor cells and their molecular biological analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20150507 |