CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents

Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDF

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Publication number
CA2876178C
CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
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CA
Canada
Prior art keywords
microorganism
biodiesel
clostridium
nucleic acid
substrate
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2876178A
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English (en)
French (fr)
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CA2876178A1 (en
Inventor
Fungmin Liew
Michael Koepke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzatech NZ Inc
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of CA2876178A1 publication Critical patent/CA2876178A1/en
Application granted granted Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2876178A 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Active CA2876178C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261662467P 2012-06-21 2012-06-21
US61/662,467 2012-06-21
PCT/NZ2013/000108 WO2013191567A1 (en) 2012-06-21 2013-06-21 Recombinant microorganisms make biodiesel

Publications (2)

Publication Number Publication Date
CA2876178A1 CA2876178A1 (en) 2013-12-27
CA2876178C true CA2876178C (en) 2017-02-28

Family

ID=49769067

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2876178A Active CA2876178C (en) 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel

Country Status (13)

Country Link
US (1) US9347076B2 (cg-RX-API-DMAC7.html)
EP (1) EP2864470B2 (cg-RX-API-DMAC7.html)
JP (1) JP6466836B2 (cg-RX-API-DMAC7.html)
KR (1) KR102097844B1 (cg-RX-API-DMAC7.html)
CN (1) CN104640977B (cg-RX-API-DMAC7.html)
BR (1) BR112014031876B1 (cg-RX-API-DMAC7.html)
CA (1) CA2876178C (cg-RX-API-DMAC7.html)
ES (1) ES2655214T5 (cg-RX-API-DMAC7.html)
IN (1) IN2014DN10507A (cg-RX-API-DMAC7.html)
MY (1) MY164882A (cg-RX-API-DMAC7.html)
NO (1) NO2953671T3 (cg-RX-API-DMAC7.html)
PL (1) PL2864470T5 (cg-RX-API-DMAC7.html)
WO (1) WO2013191567A1 (cg-RX-API-DMAC7.html)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201112231D0 (en) * 2011-07-15 2011-08-31 Verdant Bioproducts Ltd Micro-organism
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
EP3099779B1 (en) 2014-01-28 2020-09-02 LanzaTech New Zealand Limited Method of producing a recombinant microorganism
JP6309304B2 (ja) * 2014-02-24 2018-04-11 積水化学工業株式会社 組換え細胞、並びに、１，４−ブタンジオールの生産方法
PL3209786T3 (pl) 2014-10-22 2023-10-30 Lanzatech Nz, Inc. Sposoby prowadzone w reaktorach wielostopniowych
ES2946783T3 (es) 2014-10-22 2023-07-26 Lanzatech Nz Inc Unidad de pruebas de gas y método
TWI739734B (zh) 2015-02-23 2021-09-21 紐西蘭商藍瑟科技紐西蘭有限公司 用於將甲烷轉化為產物之重組產醋酸細菌
JP6871870B2 (ja) * 2015-05-27 2021-05-19 ランザテク・ニュージーランド・リミテッド コリスミ酸誘導産物生成のための遺伝子組換え微生物（関連出願の相互参照）
CA3151120A1 (en) 2015-10-13 2017-04-20 Lanzatech Nz, Inc. Genetically engineered bacterium comprising energy-generating fermentation pathway
KR20180127632A (ko) 2015-12-03 2018-11-29 란자테크 뉴질랜드 리미티드 가스 발효 아세토젠에서의 효율을 개선하기 위한 아르기닌 보충
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
WO2017136478A1 (en) 2016-02-01 2017-08-10 Lanzatech New Zealand Limited Integrated fermentation and electrolysis process
SG11201807025SA (en) 2016-02-26 2018-09-27 Lanzatech New Zealand Ltd Crispr/cas systems for c-1 fixing bacteria
JP7502029B2 (ja) 2016-05-14 2024-06-18 ランザテク，インコーポレイテッド 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法
CN107217017B (zh) * 2017-05-27 2020-12-08 青岛农业大学 一株不动杆菌及其在石油降解中的应用
PT3679149T (pt) 2017-09-08 2022-09-05 Lanzatech Inc Processos para produção de metabolitos usando substratos contendo c1 ricos em hidrogénio
JP7304859B2 (ja) 2017-12-19 2023-07-07 ランザテク，インコーポレイテッド エチレングリコールの生物生産のための微生物および方法
CN111683731B (zh) 2018-02-12 2022-09-02 朗泽科技有限公司 用于提高碳转化效率的工艺
CN111918957B (zh) 2018-04-20 2024-11-08 朗泽科技有限公司 间歇电解流
WO2020106722A1 (en) 2018-11-19 2020-05-28 Lanzatech, Inc. Integration of fermentation and gasification
CA3185501A1 (en) 2019-02-08 2020-08-13 Lanzatech, Inc. Process for recovering close boiling products
CA3138589C (en) 2019-07-11 2024-04-02 Lanzatech, Inc. Methods for optimizing gas utilization
CN111088193B (zh) * 2020-01-10 2022-09-09 南京工业大学 一种提高食甲基丁酸杆菌电转频率的方法
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
AU2021237368B2 (en) 2020-03-18 2025-05-08 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
US12134794B2 (en) 2020-04-29 2024-11-05 Lanzatech, Inc. Fermentative production of B-ketoadipate from gaseous substrates
WO2021248015A1 (en) 2020-06-06 2021-12-09 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
WO2022170191A1 (en) 2021-02-08 2022-08-11 Lanzatech, Inc. Recombinant microorganisms and uses therefor
AU2022253950B2 (en) 2021-04-09 2025-09-04 Lanzatech, Inc. Process and apparatus for providing a feedstock
WO2023004293A1 (en) 2021-07-20 2023-01-26 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
CN119452090A (zh) 2022-06-21 2025-02-14 朗泽科技有限公司 用于由c1底物连续共产生串联重复蛋白和化学产物的微生物和方法
AU2023289395A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
US12129503B2 (en) 2022-08-10 2024-10-29 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024253882A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation
US12359224B2 (en) 2023-06-05 2025-07-15 Lanzatech, Inc. Integrated gas fermentation and carbon black processes

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
PL205622B1 (pl) 2000-07-25 2010-05-31 Emmaus Foundation Ciągły sposób wytwarzania etanolu w beztlenowej fermentacji bakteryjnej
DE102004052115A1 (de) * 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
MX344588B (es) * 2006-05-19 2016-12-20 Reg Life Sciences Llc Produccion de acidos grasos y derivados de los mismos.
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
AU2008230735A1 (en) * 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
US20100199548A1 (en) * 2007-07-06 2010-08-12 Ls9, Inc. Systems and methods for the production of fatty esters
BRPI0820556B1 (pt) 2007-11-13 2016-03-22 Lanzatech New Zealand Ltd bactéria e métodos para uso das mesmas
GB0801787D0 (en) * 2008-01-31 2008-03-05 Reclaim Resources Ltd Apparatus and method for treating waste
US8048654B2 (en) * 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
WO2010022090A1 (en) * 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
BRPI1014472A8 (pt) * 2009-04-10 2015-12-08 Ls9 Inc Método para produzir um derivado de ácido graxo, microrganismo engenheirado geneticamente, derivado de ácido graxo e composição de biocombustível
BRPI1009980A2 (pt) 2009-05-01 2015-08-25 Univ California Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
WO2012026833A1 (en) * 2010-08-26 2012-03-01 Lanzatech New Zealand Limited Process for producing ethanol and ethylene via fermentation
US20110236941A1 (en) 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
TWI541348B (zh) 2011-02-25 2016-07-11 藍瑟科技紐西蘭有限公司 重組一氧化碳營養性產乙酸微生物及其用途
CN102492672A (zh) * 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel

Also Published As

Publication number Publication date
BR112014031876B1 (pt) 2022-05-10
US20130344547A1 (en) 2013-12-26
EP2864470B1 (en) 2017-10-04
PL2864470T3 (pl) 2018-05-30
KR102097844B1 (ko) 2020-04-06
EP2864470A4 (en) 2015-12-16
KR20150023651A (ko) 2015-03-05
MY164882A (en) 2018-01-30
IN2014DN10507A (cg-RX-API-DMAC7.html) 2015-08-21
JP6466836B2 (ja) 2019-02-06
CN104640977A (zh) 2015-05-20
PL2864470T5 (pl) 2021-05-17
EP2864470B2 (en) 2020-12-23
NO2953671T3 (cg-RX-API-DMAC7.html) 2018-03-17
JP2015519918A (ja) 2015-07-16
ES2655214T5 (es) 2021-10-11
EP2864470A1 (en) 2015-04-29
CA2876178A1 (en) 2013-12-27
BR112014031876A2 (pt) 2017-08-01
US9347076B2 (en) 2016-05-24
ES2655214T3 (es) 2018-02-19
WO2013191567A1 (en) 2013-12-27
CN104640977B (zh) 2019-04-12

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