CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents
Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDFInfo
- Publication number
- CA2876178C CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
- Authority
- CA
- Canada
- Prior art keywords
- microorganism
- biodiesel
- clostridium
- nucleic acid
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
- C10L1/00—Liquid carbonaceous fuels
- C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
- C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/145—Clostridium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261662467P | 2012-06-21 | 2012-06-21 | |
| US61/662,467 | 2012-06-21 | ||
| PCT/NZ2013/000108 WO2013191567A1 (en) | 2012-06-21 | 2013-06-21 | Recombinant microorganisms make biodiesel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2876178A1 CA2876178A1 (en) | 2013-12-27 |
| CA2876178C true CA2876178C (en) | 2017-02-28 |
Family
ID=49769067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2876178A Active CA2876178C (en) | 2012-06-21 | 2013-06-21 | Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9347076B2 (OSRAM) |
| EP (1) | EP2864470B2 (OSRAM) |
| JP (1) | JP6466836B2 (OSRAM) |
| KR (1) | KR102097844B1 (OSRAM) |
| CN (1) | CN104640977B (OSRAM) |
| BR (1) | BR112014031876B1 (OSRAM) |
| CA (1) | CA2876178C (OSRAM) |
| ES (1) | ES2655214T5 (OSRAM) |
| IN (1) | IN2014DN10507A (OSRAM) |
| MY (1) | MY164882A (OSRAM) |
| NO (1) | NO2953671T3 (OSRAM) |
| PL (1) | PL2864470T5 (OSRAM) |
| WO (1) | WO2013191567A1 (OSRAM) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112231D0 (en) * | 2011-07-15 | 2011-08-31 | Verdant Bioproducts Ltd | Micro-organism |
| US20130323820A1 (en) | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
| EP3099779B1 (en) | 2014-01-28 | 2020-09-02 | LanzaTech New Zealand Limited | Method of producing a recombinant microorganism |
| JP6309304B2 (ja) * | 2014-02-24 | 2018-04-11 | 積水化学工業株式会社 | 組換え細胞、並びに、１，４−ブタンジオールの生産方法 |
| JP2017531441A (ja) | 2014-10-22 | 2017-10-26 | ランザテク・ニュージーランド・リミテッド | ガス試験装置及び方法 |
| CN107002012B (zh) | 2014-10-22 | 2019-04-26 | 朗泽科技新西兰有限公司 | 多阶段生物反应器方法 |
| TWI739734B (zh) | 2015-02-23 | 2021-09-21 | 紐西蘭商藍瑟科技紐西蘭有限公司 | 用於將甲烷轉化為產物之重組產醋酸細菌 |
| CN108884467A (zh) | 2015-10-13 | 2018-11-23 | 朗泽科技新西兰有限公司 | 包含产能发酵途径的基因工程菌 |
| KR20180127632A (ko) | 2015-12-03 | 2018-11-29 | 란자테크 뉴질랜드 리미티드 | 가스 발효 아세토젠에서의 효율을 개선하기 위한 아르기닌 보충 |
| US10358661B2 (en) | 2015-12-28 | 2019-07-23 | Lanzatech New Zealand Limited | Microorganism with modified hydrogenase activity |
| CA3083080C (en) | 2016-02-01 | 2021-10-19 | Lanzatech New Zealand Limited | Integrated fermentation and electrolysis process |
| CN109072245B (zh) | 2016-02-26 | 2022-06-14 | 朗泽科技新西兰有限公司 | 用于c1固定菌的crispr/cas系统 |
| KR20230044031A (ko) | 2016-05-14 | 2023-03-31 | 란자테크, 인크. | 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법 |
| CN107217017B (zh) * | 2017-05-27 | 2020-12-08 | 青岛农业大学 | 一株不动杆菌及其在石油降解中的应用 |
| US10808263B2 (en) | 2017-09-08 | 2020-10-20 | Lanzatech, Inc. | Processes and systems for metabolite production using hydrogen rich C1-containing substrates |
| AU2018393075B2 (en) | 2017-12-19 | 2024-03-21 | Lanzatech, Inc. | Microorganisms and methods for the biological production of ethylene glycol |
| CN111683731B (zh) | 2018-02-12 | 2022-09-02 | 朗泽科技有限公司 | 用于提高碳转化效率的工艺 |
| CN111918957B (zh) | 2018-04-20 | 2024-11-08 | 朗泽科技有限公司 | 间歇电解流 |
| US11097967B2 (en) | 2018-11-19 | 2021-08-24 | Lanzatech, Inc. | Integration of fermentation and gasification |
| MY196166A (en) | 2019-02-08 | 2023-03-17 | Lanzatech Inc | Process For Recovering Close Boiling Products |
| AU2020310820B2 (en) | 2019-07-11 | 2023-10-19 | Lanzatech, Inc. | Methods for optimizing gas utilization |
| CN111088193B (zh) * | 2020-01-10 | 2022-09-09 | 南京工业大学 | 一种提高食甲基丁酸杆菌电转频率的方法 |
| US11932818B2 (en) | 2020-03-16 | 2024-03-19 | Lanzatech, Inc. | Tail gas of gas fermentation to dry gasification feedstock |
| KR20220140619A (ko) | 2020-03-18 | 2022-10-18 | 란자테크, 인크. | 가스성 기질로부터의 2-페닐에탄올의 발효 제조 |
| BR112022021838A2 (pt) | 2020-04-29 | 2022-12-13 | Lanzatech Inc | Microrganismo, método para produzir ss-cetoadipato, e, método de produção de um polímero |
| KR20230156819A (ko) | 2020-06-06 | 2023-11-14 | 란자테크, 인크. | 아세토락테이트 탈카복실화효소 유전자위에 녹인이 있는 미생물 |
| WO2022170191A1 (en) | 2021-02-08 | 2022-08-11 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| EP4320249A1 (en) | 2021-04-09 | 2024-02-14 | Lanzatech, Inc. | Process and apparatus for providing a feedstock |
| WO2023004293A1 (en) | 2021-07-20 | 2023-01-26 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| TW202307202A (zh) | 2021-08-06 | 2023-02-16 | 美商朗澤科技有限公司 | 用於改良乙二醇之生物產生的微生物及方法 |
| US12091648B2 (en) | 2021-11-03 | 2024-09-17 | Lanzatech, Inc. | System and method for generating bubbles in a vessel |
| US12280331B2 (en) | 2022-04-29 | 2025-04-22 | Lanzatech, Inc. | Low residence time gas separator |
| US12077800B2 (en) | 2022-06-16 | 2024-09-03 | Lanzatech, Inc. | Liquid distributor system and process of liquid distribution |
| CN119452090A (zh) | 2022-06-21 | 2025-02-14 | 朗泽科技有限公司 | 用于由c1底物连续共产生串联重复蛋白和化学产物的微生物和方法 |
| JP2025525381A (ja) | 2022-06-21 | 2025-08-05 | ランザテク，インコーポレイテッド | Ｃ１基質からの高価値の特殊タンパク質及び化学産物の連続共産生のための微生物及び方法 |
| WO2024036187A1 (en) | 2022-08-10 | 2024-02-15 | Lanzatech, Inc. | Carbon sequestration in soils with production of chemical products |
| US12281344B2 (en) | 2023-06-05 | 2025-04-22 | Lanzatech, Inc. | Integrated gas fermentation |
| US12359224B2 (en) | 2023-06-05 | 2025-07-15 | Lanzatech, Inc. | Integrated gas fermentation and carbon black processes |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5173429A (en) | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
| UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
| NZ523484A (en) | 2000-07-25 | 2005-01-28 | Emmaus Foundation Inc | Methods for increasing the production of ethanol from microbial fermentation |
| DE102004052115A1 (de) * | 2004-10-26 | 2006-04-27 | Westfälische Wilhelms-Universität Münster | Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung |
| ES2529176T3 (es) * | 2006-05-19 | 2015-02-17 | Ls9, Inc. | Producción de ácidos grasos y derivados de los mismos |
| US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
| EP3369807A3 (en) * | 2007-03-28 | 2018-11-21 | REG Life Sciences, LLC | Enhanced production of fatty acid derivatives |
| WO2009009391A2 (en) * | 2007-07-06 | 2009-01-15 | Ls9, Inc. | Systems and methods for the production of fatty esters |
| BRPI0820556B1 (pt) | 2007-11-13 | 2016-03-22 | Lanzatech New Zealand Ltd | bactéria e métodos para uso das mesmas |
| GB0801787D0 (en) * | 2008-01-31 | 2008-03-05 | Reclaim Resources Ltd | Apparatus and method for treating waste |
| US8048654B2 (en) * | 2010-06-09 | 2011-11-01 | Joule Unlimited Technologies, Inc. | Methods and compositions for the recombinant biosynthesis of fatty acids and esters |
| WO2010021711A1 (en) * | 2008-08-18 | 2010-02-25 | Ls9, Inc. | Systems and methods for the production of mixed fatty esters |
| BRPI1014472A8 (pt) * | 2009-04-10 | 2015-12-08 | Ls9 Inc | Método para produzir um derivado de ácido graxo, microrganismo engenheirado geneticamente, derivado de ácido graxo e composição de biocombustível |
| WO2010127318A2 (en) | 2009-05-01 | 2010-11-04 | The Regents Of The University Of California | Product of fatty acid esters from biomass polymers |
| US8143037B2 (en) | 2010-03-19 | 2012-03-27 | Coskata, Inc. | Ethanologenic Clostridium species, Clostridium coskatii |
| WO2012026833A1 (en) * | 2010-08-26 | 2012-03-01 | Lanzatech New Zealand Limited | Process for producing ethanol and ethylene via fermentation |
| US20110236941A1 (en) | 2010-10-22 | 2011-09-29 | Lanzatech New Zealand Limited | Recombinant microorganism and methods of production thereof |
| ES2702621T3 (es) | 2011-02-25 | 2019-03-04 | Lanzatech New Zealand Ltd | Microorganismos recombinantes y sus usos |
| CN102492672A (zh) * | 2011-11-26 | 2012-06-13 | 宁波大学 | 一种催化二酰甘油酰基化的酶 |
| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
-
2013
- 2013-06-20 US US13/923,352 patent/US9347076B2/en active Active
- 2013-06-21 JP JP2015518359A patent/JP6466836B2/ja active Active
- 2013-06-21 BR BR112014031876-0A patent/BR112014031876B1/pt active IP Right Grant
- 2013-06-21 PL PL13806177T patent/PL2864470T5/pl unknown
- 2013-06-21 WO PCT/NZ2013/000108 patent/WO2013191567A1/en active Application Filing
- 2013-06-21 CN CN201380044451.9A patent/CN104640977B/zh active Active
- 2013-06-21 IN IN10507DEN2014 patent/IN2014DN10507A/en unknown
- 2013-06-21 EP EP13806177.5A patent/EP2864470B2/en active Active
- 2013-06-21 ES ES13806177T patent/ES2655214T5/es active Active
- 2013-06-21 KR KR1020157000238A patent/KR102097844B1/ko active Active
- 2013-06-21 MY MYPI2014003406A patent/MY164882A/en unknown
- 2013-06-21 CA CA2876178A patent/CA2876178C/en active Active
-
2014
- 2014-02-05 NO NO14703269A patent/NO2953671T3/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO2953671T3 (OSRAM) | 2018-03-17 |
| PL2864470T3 (pl) | 2018-05-30 |
| EP2864470A4 (en) | 2015-12-16 |
| CA2876178A1 (en) | 2013-12-27 |
| EP2864470B1 (en) | 2017-10-04 |
| KR20150023651A (ko) | 2015-03-05 |
| BR112014031876A2 (pt) | 2017-08-01 |
| ES2655214T5 (es) | 2021-10-11 |
| PL2864470T5 (pl) | 2021-05-17 |
| EP2864470A1 (en) | 2015-04-29 |
| KR102097844B1 (ko) | 2020-04-06 |
| JP6466836B2 (ja) | 2019-02-06 |
| IN2014DN10507A (OSRAM) | 2015-08-21 |
| WO2013191567A1 (en) | 2013-12-27 |
| US20130344547A1 (en) | 2013-12-26 |
| ES2655214T3 (es) | 2018-02-19 |
| CN104640977A (zh) | 2015-05-20 |
| EP2864470B2 (en) | 2020-12-23 |
| MY164882A (en) | 2018-01-30 |
| JP2015519918A (ja) | 2015-07-16 |
| US9347076B2 (en) | 2016-05-24 |
| CN104640977B (zh) | 2019-04-12 |
| BR112014031876B1 (pt) | 2022-05-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20141209 |