CA2874832C - Recombinant microorganisms and uses therefor - Google Patents

Recombinant microorganisms and uses therefor Download PDF

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Publication number
CA2874832C
CA2874832C CA2874832A CA2874832A CA2874832C CA 2874832 C CA2874832 C CA 2874832C CA 2874832 A CA2874832 A CA 2874832A CA 2874832 A CA2874832 A CA 2874832A CA 2874832 C CA2874832 C CA 2874832C
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Canada
Prior art keywords
bacteria
clostridium
microorganism
nucleic acid
carboxydotrophic
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CA2874832A
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English (en)
French (fr)
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CA2874832A1 (en
Inventor
Wendy Chen
Michael Koepke
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Lanzatech NZ Inc
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Lanzatech New Zealand Ltd
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Publication of CA2874832A1 publication Critical patent/CA2874832A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2874832A 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor Active CA2874832C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261653348P 2012-05-30 2012-05-30
US61/653,348 2012-05-30
PCT/NZ2013/000092 WO2013180581A1 (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Publications (2)

Publication Number Publication Date
CA2874832A1 CA2874832A1 (en) 2013-12-05
CA2874832C true CA2874832C (en) 2016-02-02

Family

ID=49670706

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2874832A Active CA2874832C (en) 2012-05-30 2013-05-30 Recombinant microorganisms and uses therefor

Country Status (8)

Country Link
US (1) US9994878B2 (en:Method)
EP (1) EP2855690B1 (en:Method)
JP (1) JP6445970B2 (en:Method)
KR (1) KR102098849B1 (en:Method)
CN (2) CN104508136A (en:Method)
CA (1) CA2874832C (en:Method)
IN (1) IN2014DN10168A (en:Method)
WO (1) WO2013180581A1 (en:Method)

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CA2936251C (en) 2014-01-28 2017-11-07 Lanzatech New Zealand Limited Method of producing a recombinant microorganism
JP2017531441A (ja) 2014-10-22 2017-10-26 ランザテク・ニュージーランド・リミテッド ガス試験装置及び方法
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WO2016138050A1 (en) 2015-02-23 2016-09-01 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
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DE102016212497B4 (de) * 2015-07-13 2024-04-25 Sk Innovation Co., Ltd. Mutanter Mikroorganismus, der ein Gen umfasst, das Methylmalonyl-CoA-Reductase codiert, und seine Verwendung
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US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
GB201601558D0 (en) 2016-01-28 2016-03-16 Verdant Bioproducts Ltd Method for producing 3-hydroxypropionamide
ES2955708T3 (es) 2016-02-01 2023-12-05 Lanzatech Nz Inc Proceso integrado de fermentación y electrólisis
AU2017222688B2 (en) 2016-02-26 2023-05-25 Lanzatech Nz, Inc. CRISPR/Cas systems for C-1 fixing bacteria
US10294498B2 (en) 2016-05-14 2019-05-21 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
US20190233854A1 (en) * 2016-10-13 2019-08-01 Sekisui Chemical Co., Ltd. Obligately anaerobic acetic acid-producing microorganism, and recombinant microorganism
WO2019051069A1 (en) 2017-09-08 2019-03-14 Lanzatech, Inc. METHODS AND SYSTEMS FOR PRODUCING METABOLITES USING SUBSTRATES CONTAINING C1 COMPOUNDS RICH IN HYDROGEN
CN111936631A (zh) 2017-12-19 2020-11-13 朗泽科技有限公司 用于生物产生乙二醇的微生物和方法
WO2019157507A1 (en) 2018-02-12 2019-08-15 Lanzatech, Inc. A process for improving carbon conversion efficiency
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CN109112156A (zh) * 2018-09-19 2019-01-01 江苏师范大学 一种生产3-羟基丙酸的重组假单胞菌、其构建方法及其应用
KR102549843B1 (ko) 2018-11-19 2023-06-29 란자테크, 인크. 발효와 가스화의 통합
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EP3997235A4 (en) 2019-07-11 2024-05-08 Lanzatech, Inc. PROCESS FOR OPTIMIZING GAS USE
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
AU2021237368B2 (en) 2020-03-18 2025-05-08 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
WO2021222265A1 (en) * 2020-04-29 2021-11-04 Lanzatech, Inc. FERMENTATIVE PRODUCTION OF β-KETOADIPATE FROM GASEOUS SUBSTRATES
US11760989B2 (en) 2020-06-06 2023-09-19 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
CN116348603B (zh) * 2021-02-08 2024-07-02 朗泽科技有限公司 重组微生物及其用途
CN117098850A (zh) 2021-04-09 2023-11-21 朗泽科技有限公司 用于提供原料的方法和设备
WO2023004293A1 (en) 2021-07-20 2023-01-26 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
AU2023286621A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates
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Also Published As

Publication number Publication date
CN113832199A (zh) 2021-12-24
CA2874832A1 (en) 2013-12-05
KR102098849B1 (ko) 2020-04-09
KR20150021081A (ko) 2015-02-27
WO2013180581A1 (en) 2013-12-05
EP2855690A1 (en) 2015-04-08
EP2855690A4 (en) 2016-02-17
JP6445970B2 (ja) 2018-12-26
US20130323806A1 (en) 2013-12-05
CN104508136A (zh) 2015-04-08
JP2015517823A (ja) 2015-06-25
EP2855690B1 (en) 2018-07-11
US9994878B2 (en) 2018-06-12
IN2014DN10168A (en:Method) 2015-08-21

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