CA2874343C - Single-particle analysis of particle populations - Google Patents

Single-particle analysis of particle populations Download PDF

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Publication number
CA2874343C
CA2874343C CA2874343A CA2874343A CA2874343C CA 2874343 C CA2874343 C CA 2874343C CA 2874343 A CA2874343 A CA 2874343A CA 2874343 A CA2874343 A CA 2874343A CA 2874343 C CA2874343 C CA 2874343C
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particles
particle
amplification
capture
primer
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English (en)
French (fr)
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CA2874343A1 (en
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Megan Anderson
Peilin Chen
Brian Fowler
Fiona Kaper
Ronald Lebofsky
Andrew May
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Standard Biotools Inc
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Fluidigm Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA2874343A 2012-05-21 2013-05-21 Single-particle analysis of particle populations Active CA2874343C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261649845P 2012-05-21 2012-05-21
US61/649,845 2012-05-21
PCT/US2013/042086 WO2013177206A2 (en) 2012-05-21 2013-05-21 Single-particle analysis of particle populations

Publications (2)

Publication Number Publication Date
CA2874343A1 CA2874343A1 (en) 2013-11-28
CA2874343C true CA2874343C (en) 2021-11-09

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Family Applications (1)

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CA2874343A Active CA2874343C (en) 2012-05-21 2013-05-21 Single-particle analysis of particle populations

Country Status (7)

Country Link
US (1) US9840732B2 (https=)
EP (1) EP2852682B1 (https=)
JP (1) JP2015519900A (https=)
CN (1) CN104471077B (https=)
CA (1) CA2874343C (https=)
SG (1) SG11201407901PA (https=)
WO (1) WO2013177206A2 (https=)

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101829182B1 (ko) 2009-04-02 2018-03-29 플루이다임 코포레이션 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
MX2012001214A (es) * 2009-07-29 2012-06-25 Pyrobett Pte Ltd Metodo y aparato para conducir un ensayo.
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US10457936B2 (en) 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
EP2739587B1 (en) 2011-08-01 2020-05-27 Denovo Sciences Cell capture system
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9752181B2 (en) 2013-01-26 2017-09-05 Denovo Sciences, Inc. System and method for capturing and analyzing cells
WO2014142850A1 (en) * 2013-03-13 2014-09-18 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9856535B2 (en) 2013-05-31 2018-01-02 Denovo Sciences, Inc. System for isolating cells
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
CA2935122C (en) * 2013-12-30 2023-09-19 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
US10526601B2 (en) * 2014-05-23 2020-01-07 Digenomix Corporation Haploidome determination by digitized transposons
US10118173B2 (en) 2014-10-09 2018-11-06 Illumina, Inc. Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
US11873480B2 (en) 2014-10-17 2024-01-16 Illumina Cambridge Limited Contiguity preserving transposition
US10227650B2 (en) * 2014-11-14 2019-03-12 Athena Diagnostics, Inc. Methods to detect a silent carrier of a null allele genotype
CN107406886A (zh) * 2015-01-23 2017-11-28 哈佛学院院长及董事 用于在液滴内扩增或克隆的系统、方法和试剂盒
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
JP2018509896A (ja) 2015-02-27 2018-04-12 フリューダイム・コーポレイション 高処理能力研究用の単一細胞核酸
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
WO2017117358A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Digital protein quantification
WO2017120531A1 (en) * 2016-01-08 2017-07-13 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US11285478B2 (en) 2016-04-04 2022-03-29 Combinati Incorporated Microfluidic siphoning array for nucleic acid quantification
JP2019515674A (ja) 2016-04-19 2019-06-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
CN117056774B (zh) 2016-11-08 2026-01-27 贝克顿迪金森公司 用于细胞标记分类的方法
CN109952612B (zh) 2016-11-08 2023-12-01 贝克顿迪金森公司 用于表达谱分类的方法
ES2909055T3 (es) * 2016-11-17 2022-05-05 Combinati Incorporated Métodos y sistemas para el análisis y la cuantificación de ácidos nucleicos
CN110573253B (zh) 2017-01-13 2021-11-02 赛卢拉研究公司 流体通道的亲水涂层
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
LT3375889T (lt) * 2017-03-17 2020-06-25 Hifibio Sas Vienos ląstelės analizė
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
WO2019046307A1 (en) 2017-08-29 2019-03-07 Celsee Diagnostics, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
WO2019060685A1 (en) * 2017-09-22 2019-03-28 Bioventures, Llc SYSTEM AND METHOD FOR PHENOTYPE ANALYSIS AND POLYNUCLEOTIDE SEQUENCING OF BIOLOGICAL PARTICLES THROUGH A DETERMINISTIC BARCODE
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
WO2019241249A1 (en) * 2018-06-11 2019-12-19 The Brigham And Women's Hospital, Inc. Single cell cloning approaches for biological studies
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
AU2019397371B2 (en) 2018-12-10 2025-09-18 Combinati Incorporated Microfluidic array for sample digitization
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
EP3966307B1 (en) 2019-05-07 2024-11-20 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
EP3969604B1 (en) * 2019-05-13 2026-03-18 Vrije Universiteit Brussel Methods and systems for particle characterisation
JP2022535300A (ja) 2019-06-07 2022-08-05 ハニカム・バイオテクノロジーズ・インコーポレーテッド 生物学的試料の収集、保存、及び分析のための試料容器カセット
SG11202112898WA (en) 2019-06-14 2021-12-30 Bio Rad Laboratories System and method for automated single cell processing and analyses
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
IT201900024159A1 (it) * 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
CN111537480B (zh) * 2020-04-26 2023-05-02 中央民族大学 基于单分子全内反射荧光成像技术的病毒快速检测方法
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CA3195362A1 (en) 2020-09-28 2022-03-31 Combinati Incorporated Devices and methods for sample processing
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
CN113881807A (zh) * 2021-10-14 2022-01-04 杭州柏熠科技有限公司 甲型流感病毒全基因组扩增试剂盒及扩增方法和应用
WO2023107539A1 (en) 2021-12-10 2023-06-15 Bio-Rad Laboratories, Inc. Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles
TWI868818B (zh) * 2022-07-25 2025-01-01 國立中央大學 微流道檢測裝置
EP4662310A2 (en) * 2023-02-07 2025-12-17 Parse Biosciences, Inc. Methods and kits for labeling cellular molecules for multiplex analysis
EP4662478A1 (en) * 2023-02-10 2025-12-17 IDEXX Laboratories, Inc. Methods, systems, and devices for linear electrowetting cartridges
CN118376611B (zh) * 2024-04-19 2025-08-15 北京航空航天大学 单纳米颗粒表面电势的测量装置以及测量方法

Family Cites Families (191)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4038804A1 (de) 1990-10-09 1992-04-16 Boehringer Mannheim Gmbh Verfahren zur genus- oder/und spezies-spezifischen detektion von bakterien in einer probenfluessigkeit
US5272081A (en) 1982-05-10 1993-12-21 Bar-Ilan University System and methods for cell selection
US4508625A (en) 1982-10-18 1985-04-02 Graham Marshall D Magnetic separation using chelated magnetic ions
EP0197196A1 (en) 1985-03-08 1986-10-15 The University Of Rochester Electro-electron optical oscilloscope system for time-resolving picosecond electrical waveforms
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4822733A (en) 1985-05-28 1989-04-18 Amoco Corporation Lifetime-resolved assay procedures
US5164598A (en) 1985-08-05 1992-11-17 Biotrack Capillary flow device
US5721098A (en) 1986-01-16 1998-02-24 The Regents Of The University Of California Comparative genomic hybridization
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5641628A (en) 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
JPH05507404A (ja) 1990-04-23 1993-10-28 セルプロ インコーポレイティド 母体血液からの胎児細胞の富化のための方法
WO1992004458A1 (en) 1990-09-11 1992-03-19 The Brookdale Hospital Medical Center Drug resistance screening method
WO1993007296A1 (en) 1991-10-03 1993-04-15 Indiana University Foundation Method for screening for alzheimer's disease
EP0576842B1 (de) 1992-06-11 2002-10-16 MERCK PATENT GmbH Verfahren und Mittel zum Nachweis von Listerien
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
US5840502A (en) 1994-08-31 1998-11-24 Activated Cell Therapy, Inc. Methods for enriching specific cell-types by density gradient centrifugation
US5750339A (en) 1994-11-30 1998-05-12 Thomas Jefferson University Methods for identifying fetal cells
AU710425B2 (en) 1995-12-18 1999-09-23 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
EP1736554B1 (en) 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
IT1294964B1 (it) 1996-07-12 1999-04-23 Domenico Valerio Isolamento e cultura di cellule fetali dal sangue periferico materno
US6100029A (en) 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US6300077B1 (en) 1996-08-14 2001-10-09 Exact Sciences Corporation Methods for the detection of nucleic acids
JP2002503334A (ja) 1996-09-04 2002-01-29 テクニカル ユニバーシティ オブ デンマーク 粒子の分離と分析用のマイクロフローシステム
GB9704444D0 (en) 1997-03-04 1997-04-23 Isis Innovation Non-invasive prenatal diagnosis
US5965375A (en) 1997-04-04 1999-10-12 Biosite Diagnostics Diagnostic tests and kits for Clostridium difficile
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6368871B1 (en) 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
AU758463B2 (en) 1998-02-04 2003-03-20 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
DE19813317A1 (de) 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
US6387652B1 (en) 1998-04-15 2002-05-14 U.S. Environmental Protection Agency Method of identifying and quantifying specific fungi and bacteria
US6045993A (en) 1998-05-30 2000-04-04 Visible Genetics Inc. Method, reagent and kit for genotyping of human papillomavirus
US20030022207A1 (en) 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
AU5196199A (en) 1998-08-11 2000-03-06 Asahi Breweries, Ltd. Genes for detecting bacteria and detection method by using the same
US6656697B1 (en) 1998-09-28 2003-12-02 Lifescan, Inc. Diagnostics based on tetrazolium compounds
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US6511967B1 (en) 1999-04-23 2003-01-28 The General Hospital Corporation Use of an internalizing transferrin receptor to image transgene expression
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
IL147302A0 (en) 1999-06-28 2002-08-14 California Inst Of Techn Microfabricated elastomeric valve and pump systems
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6723505B1 (en) 1999-08-13 2004-04-20 Nye Colifast As Method for identification of the indicators of contamination in liquid samples
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
MXPA02002656A (es) 1999-09-13 2003-10-14 Nugen Technologies Inc Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos.
US6618679B2 (en) 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
US20010026919A1 (en) 2000-02-08 2001-10-04 Alex Chenchik Nucleic acid assays employing universal arrays
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
CA2413158A1 (en) 2000-06-30 2002-01-10 Incyte Genomics, Inc. Ecm-related tumor marker
US6984522B2 (en) 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
EP1309863A1 (en) 2000-08-08 2003-05-14 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
US20040005582A1 (en) 2000-08-10 2004-01-08 Nanobiodynamics, Incorporated Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators
WO2002014867A2 (en) 2000-08-11 2002-02-21 Agilix Corporation Ultra-sensitive detection systems
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
GB0026424D0 (en) 2000-10-28 2000-12-13 Ncimb Ltd Genetic analysis of microorganisms
EP1368369A4 (en) 2000-11-15 2006-02-22 Hoffmann La Roche METHOD AND REAGENTS FOR IDENTIFYING RARE FOETAL CELLS IN THE MATERIAL CIRCUIT
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US20020155587A1 (en) 2001-04-20 2002-10-24 Sequenom, Inc. System and method for testing a biological sample
FR2824144B1 (fr) 2001-04-30 2004-09-17 Metagenex S A R L Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel
US20020166760A1 (en) 2001-05-11 2002-11-14 Prentiss Mara G. Micromagentic systems and methods for microfluidics
US6783928B2 (en) 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
WO2003008931A2 (en) 2001-07-17 2003-01-30 Georgi Hvichia Microstructure for particle and cell separation, identification, sorting, and manipulation
US7306901B2 (en) 2001-08-08 2007-12-11 Tibotec Pharmaceuticals, Ltd. Methods and means for assessing HIV envelope inhibitor therapy
US6927028B2 (en) 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
US7153656B2 (en) 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
US7166443B2 (en) 2001-10-11 2007-01-23 Aviva Biosciences Corporation Methods, compositions, and automated systems for separating rare cells from fluid samples
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
WO2003044217A2 (en) 2001-11-20 2003-05-30 Exact Sciences Corporation Automated sample preparation methods and devices
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US20030119004A1 (en) 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US20040110166A1 (en) 2002-03-07 2004-06-10 Macevicz Stephen C. Genome-wide scanning of genetic polymorphisms
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
CA2480728A1 (en) * 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
US7727720B2 (en) 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20060086309A1 (en) 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
US7106905B2 (en) 2002-08-23 2006-09-12 Hewlett-Packard Development Company, L.P. Systems and methods for processing text-based electronic documents
CN1703521B (zh) 2002-09-06 2011-11-16 波士顿大学信托人 基因表达的定量
CA2499245A1 (en) * 2002-09-16 2004-03-25 Cytonome, Inc. Method and apparatus for sorting particles
US7153658B2 (en) 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
CA2500392C (en) 2002-09-27 2012-11-27 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
AU2003299541A1 (en) 2002-10-02 2004-05-25 California Institute Of Technology Microfluidic nucleic acid analysis
EP1585583B1 (en) 2002-10-23 2010-04-07 The Trustees of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
US7704687B2 (en) 2002-11-15 2010-04-27 The Johns Hopkins University Digital karyotyping
JPWO2004051231A1 (ja) 2002-11-29 2006-04-06 日本電気株式会社 分離装置および分離方法
US8323897B2 (en) 2002-12-04 2012-12-04 Applied Biosystems, Llc Multiplex amplification of polynucleotides
US20040110153A1 (en) 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification
WO2004065629A1 (en) 2003-01-17 2004-08-05 The Chinese University Of Hong Kong Circulating mrna as diagnostic markers for pregnancy-related disorders
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
AU2003901671A0 (en) 2003-04-02 2003-05-01 The University Of Adelaide Comparative genomic hybridization
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
EP1636564A1 (en) 2003-06-13 2006-03-22 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
AU2004254367B2 (en) 2003-06-27 2008-06-19 Nanosphere, Inc. Bio-barcode based detection of target analytes
EP2354253A3 (en) 2003-09-05 2011-11-16 Trustees of Boston University Method for non-invasive prenatal diagnosis
DE602004026077D1 (de) 2003-09-10 2010-04-29 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
KR100571817B1 (ko) 2003-09-19 2006-04-17 삼성전자주식회사 태그 서열에 혼성화하는 검출 프로브를 이용하는 표적핵산의 검출방법
CN1997691B (zh) 2003-09-23 2011-07-20 北卡罗来纳大学查珀尔希尔分校 光固化的全氟聚醚用作微流体器件中的新材料
CN1898395B (zh) 2003-10-13 2014-02-12 戴尔斯瑞克斯实验室 基于引物的核酸的多重扩增的方法和试剂盒
CA2542656A1 (en) 2003-10-16 2005-05-06 Genomic Health, Inc. Qrt-pcr assay system for gene expression profiling
DE60328193D1 (de) 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
US20050221341A1 (en) 2003-10-22 2005-10-06 Shimkets Richard A Sequence-based karyotyping
US7632640B2 (en) 2003-12-08 2009-12-15 The Clinic For Special Children Association of TSPYL polymorphisms with SIDDT syndrome
WO2005064020A1 (en) 2003-12-23 2005-07-14 Autogenomics, Inc. Multiplexed nucleic acid analysis with high specificity
CN101189271A (zh) 2004-02-13 2008-05-28 北卡罗来纳大学查珀尔希尔分校 制造微流体设备的功能材料和新型方法
US20060046258A1 (en) 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20060141518A1 (en) 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
ATE399884T1 (de) 2004-03-24 2008-07-15 Applera Corp Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden
WO2005107938A2 (en) 2004-05-02 2005-11-17 Fluidigm Corporation Thermal reaction device and method for using the same
US7468249B2 (en) 2004-05-05 2008-12-23 Biocept, Inc. Detection of chromosomal disorders
JP2007536522A (ja) 2004-05-07 2007-12-13 ベックマン コールター インコーポレーティッド Ctlの媒介による抗原提示細胞の溶解を検出するためのmhc架橋系
US20060053503A1 (en) 2004-07-30 2006-03-09 Ut-Battelle, Llc Cranial and vertebral defects associated with loss-of-function of Nell
CN101124337B (zh) 2004-08-24 2010-11-17 康乃尔研究基金会有限公司 使用内切核酸酶剪切/连接酶重新密封反应和毛细管电泳或微阵列检测核酸差异
US20060051265A1 (en) 2004-09-08 2006-03-09 Health Research, Inc. Apparatus and method for sorting microstructures in a fluid medium
CA2601221C (en) 2005-03-18 2013-08-06 The Chinese University Of Hong Kong A method for the detection of chromosomal aneuploidies
CA2601735C (en) 2005-03-18 2015-10-06 The Chinese University Of Hong Kong Markers for prenatal diagnosis and monitoring of trisomy 21
US20060223178A1 (en) 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
GB0508983D0 (en) * 2005-05-03 2005-06-08 Oxford Gene Tech Ip Ltd Cell analyser
WO2006128010A2 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
EP1896617B1 (en) 2005-05-31 2013-01-02 Life Technologies Corporation Multiplex amplification of short nucleic acids
EP2477029A1 (en) 2005-06-02 2012-07-18 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US20070059680A1 (en) 2005-09-15 2007-03-15 Ravi Kapur System for cell enrichment
WO2007024800A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
WO2007033385A2 (en) 2005-09-13 2007-03-22 Fluidigm Corporation Microfluidic assay devices and methods
US20070059716A1 (en) 2005-09-15 2007-03-15 Ulysses Balis Methods for detecting fetal abnormality
US20070059719A1 (en) 2005-09-15 2007-03-15 Michael Grisham Business methods for prenatal Diagnosis
US20070059781A1 (en) 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
US20070059774A1 (en) 2005-09-15 2007-03-15 Michael Grisham Kits for Prenatal Testing
US20070059683A1 (en) 2005-09-15 2007-03-15 Tom Barber Veterinary diagnostic system
US20090170713A1 (en) 2005-09-29 2009-07-02 Keygene N.V. High throughput screening of mutagenized populations
DE602006018352D1 (de) 2005-12-06 2010-12-30 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
TR201910868T4 (tr) 2006-02-02 2019-08-21 Univ Leland Stanford Junior Dijital analizle invazif olmayan fetal genetik tarama.
EP1994179A2 (en) 2006-02-18 2008-11-26 Michael Strathmann Massively multiplexed sequencing
EP2351858B1 (en) 2006-02-28 2014-12-31 University of Louisville Research Foundation Detecting fetal chromosomal abnormalities using tandem single nucleotide polymorphisms
JP4987885B2 (ja) 2006-03-09 2012-07-25 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ 小滴中で反応を行うための装置及びその使用方法
ES2301342B1 (es) 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
US20080038733A1 (en) 2006-03-28 2008-02-14 Baylor College Of Medicine Screening for down syndrome
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
EP2040843B1 (en) 2006-06-01 2020-02-26 The Trustees of Princeton University Apparatus for continuous particle separation
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080090239A1 (en) 2006-06-14 2008-04-17 Daniel Shoemaker Rare cell analysis using sample splitting and dna tags
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
WO2009035447A1 (en) 2006-06-14 2009-03-19 Living Microsystems, Inc. Diagnosis of fetal abnormalities by comparative genomic hybridization analysis
WO2007147074A2 (en) 2006-06-14 2007-12-21 Living Microsystems, Inc. Use of highly parallel snp genotyping for fetal diagnosis
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
AU2007260750A1 (en) 2006-06-16 2007-12-21 Sequenom, Inc. Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample
WO2007149789A2 (en) 2006-06-22 2007-12-27 Applera Corporation Conversion of target specific amplification to universal sequencing
WO2008015396A2 (en) 2006-07-31 2008-02-07 Solexa Limited Method of library preparation avoiding the formation of adaptor dimers
US8603749B2 (en) 2006-11-15 2013-12-10 Biospherex, LLC Multitag sequencing ecogenomics analysis-US
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
CN101067156A (zh) 2007-05-18 2007-11-07 中国人民解放军第三军医大学第一附属医院 一种基于选择性探针的多重pcr方法及其应用
LT2557517T (lt) 2007-07-23 2023-01-10 The Chinese University Of Hong Kong Nukleino rūgščių sekos disbalanso nustatymas
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
WO2009021215A1 (en) 2007-08-09 2009-02-12 Celula, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
CA2698545C (en) 2007-09-07 2014-07-08 Fluidigm Corporation Copy number variation determination, methods and systems
WO2009036525A2 (en) 2007-09-21 2009-03-26 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
US20090163366A1 (en) 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
CN102015522B (zh) 2008-02-22 2013-07-03 弗卢迪格姆公司 用于微流体器件的集成载体
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
CN102056838B (zh) 2008-04-11 2013-07-03 弗卢丁公司 微流体装置和方法
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
CA2734868C (en) 2008-08-26 2019-09-10 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
CN101750476B (zh) * 2008-12-08 2015-06-03 深圳迈瑞生物医疗电子股份有限公司 血液分析试剂及其使用方法
CN102348811A (zh) * 2009-01-13 2012-02-08 弗卢伊蒂格姆公司 单细胞核酸分析
US8450063B2 (en) 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
WO2010115044A2 (en) 2009-04-02 2010-10-07 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
KR101829182B1 (ko) 2009-04-02 2018-03-29 플루이다임 코포레이션 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법
US20110143955A1 (en) 2009-10-11 2011-06-16 Weiner Michael P Protein Capture, Detection and Quantitation
US20140148348A1 (en) 2010-01-13 2014-05-29 Christine Kuslich Dectection of gastrointestinal disorders
WO2011143659A2 (en) 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US20140186827A1 (en) 2010-05-14 2014-07-03 Fluidigm, Inc. Assays for the detection of genotype, mutations, and/or aneuploidy
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
EP2852682B1 (en) 2012-05-21 2017-10-04 Fluidigm Corporation Single-particle analysis of particle populations

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