CA2853917A1 - Method for the detection of free binding partner of a multispecific binder - Google Patents
Method for the detection of free binding partner of a multispecific binder Download PDFInfo
- Publication number
- CA2853917A1 CA2853917A1 CA2853917A CA2853917A CA2853917A1 CA 2853917 A1 CA2853917 A1 CA 2853917A1 CA 2853917 A CA2853917 A CA 2853917A CA 2853917 A CA2853917 A CA 2853917A CA 2853917 A1 CA2853917 A1 CA 2853917A1
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- antigen
- multispecific
- sample
- idiotypic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11194350 | 2011-12-19 | ||
| EP11194350.2 | 2011-12-19 | ||
| PCT/EP2012/075991 WO2013092611A2 (en) | 2011-12-19 | 2012-12-18 | Method for the detection of free binding partner of a multispecific binder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2853917A1 true CA2853917A1 (en) | 2013-06-27 |
Family
ID=47429825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2853917A Abandoned CA2853917A1 (en) | 2011-12-19 | 2012-12-18 | Method for the detection of free binding partner of a multispecific binder |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9671394B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2795335B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6203747B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR20140099277A (cg-RX-API-DMAC7.html) |
| CN (1) | CN104040350B (cg-RX-API-DMAC7.html) |
| BR (1) | BR112014011535A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2853917A1 (cg-RX-API-DMAC7.html) |
| MX (1) | MX349003B (cg-RX-API-DMAC7.html) |
| RU (1) | RU2633488C2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013092611A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8956859B1 (en) | 2010-08-13 | 2015-02-17 | Aviex Technologies Llc | Compositions and methods for determining successful immunization by one or more vaccines |
| CN104105966B (zh) | 2012-02-01 | 2016-10-26 | 弗·哈夫曼-拉罗切有限公司 | 用于检测多特异性结合物的结合搭档的方法 |
| BR112015000665A2 (pt) | 2012-07-13 | 2017-06-27 | Hoffmann La Roche | método para a determinação da quantidade de um anticorpo |
| CA2926306C (en) * | 2013-11-05 | 2022-12-06 | F. Hoffmann-La Roche Ag | A method for determining the total amount and/or concentration of an analyte in a sample |
| WO2015143277A1 (en) * | 2014-03-20 | 2015-09-24 | Wellstat Diagnostics, Llc | Antibodies and methods for the detection of cell death |
| ES2761427T3 (es) | 2014-12-18 | 2020-05-19 | Hoffmann La Roche | Procedimientos para reducir interferencias |
| AU2018304711B2 (en) | 2017-07-20 | 2025-04-10 | Cytomx Therapeutics, Inc. | Methods of qualitatively and/or quantitatively analyzing properties of activatable antibodies and uses thereof |
| CN109358192B (zh) * | 2018-02-08 | 2020-05-12 | 中国科学院上海药物研究所 | 一种去除抗药抗体检测样品中游离药物的装置和方法、该装置的制备方法及应用 |
| EP3805757A4 (en) | 2018-06-04 | 2022-03-23 | Chugai Seiyaku Kabushiki Kaisha | Method for detecting complex |
| WO2020229378A1 (en) * | 2019-05-13 | 2020-11-19 | F. Hoffmann-La Roche Ag | Interference-suppressed pharmacokinetic immunoassay |
| KR20220007586A (ko) * | 2019-05-13 | 2022-01-18 | 리제너론 파마슈티칼스 인코포레이티드 | 개선된 경쟁적 리간드 결합 검정 |
| CN117805397A (zh) * | 2024-02-29 | 2024-04-02 | 军科正源（北京）药物研究有限责任公司 | 检测游离vegf的方法 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56128722A (en) * | 1980-03-14 | 1981-10-08 | Terumo Corp | Removal of antiacetylcholinereceptor antibody and device therefor |
| US5219730A (en) | 1982-09-28 | 1993-06-15 | New York University | Idiotype-anti-idiotype immunoassay |
| US4828981A (en) | 1983-08-24 | 1989-05-09 | Synbiotics Corporation | Immunoassays for determining Dirofilaria immitis infection using antiidiotype monoclonal antibody reagents |
| EP0170302A1 (fr) | 1984-06-27 | 1986-02-05 | l'Association internationale à but scientifique, dite: Institut international de pathologie cellulaire et moléculaire | Procédé de dosage immunologique d'une substance dans un échantillon liquide au moyen d'anticorps anti-idiotypiques |
| US4699880A (en) | 1984-09-25 | 1987-10-13 | Immunomedics, Inc. | Method of producing monoclonal anti-idiotype antibody |
| US5238808A (en) | 1984-10-31 | 1993-08-24 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| WO1987002778A1 (en) | 1985-10-22 | 1987-05-07 | Cooper-Lipotech | Solid-phase liposome immunoassay system |
| ZA898290B (en) | 1988-11-03 | 1991-06-26 | Igen Inc | Electrochemiluminescent assays |
| US5068088A (en) | 1988-11-03 | 1991-11-26 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescent measurements |
| IL100866A (en) | 1991-02-06 | 1995-10-31 | Igen Inc | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| DE69332377T2 (de) | 1992-07-13 | 2003-07-03 | Bionebraska, Inc. | Verfahren zur modifizierung rekombinanter polypeptide |
| GB9316369D0 (en) * | 1993-08-06 | 1993-09-22 | Surface Active Ltd | Diagnostic method |
| JPH11352129A (ja) * | 1998-06-04 | 1999-12-24 | Mizuho Medeii:Kk | 検出装置及び検出方法 |
| US6753189B1 (en) * | 1998-06-04 | 2004-06-22 | Mizuho Medy Co., Ltd. | Detection apparatus and method for the same |
| EP1230552B1 (en) * | 1999-11-16 | 2006-01-11 | Genentech, Inc. | Elisa for vegf |
| EP1288308A1 (en) * | 2001-08-28 | 2003-03-05 | Roche Diagnostics GmbH | A method for the determination of multiple analytes |
| GB0320459D0 (en) | 2003-09-01 | 2003-10-01 | Selective Antibodies Ltd | Assay methods and materials |
| DE102004052729A1 (de) * | 2004-10-30 | 2006-05-04 | Roche Diagnostics Gmbh | Immunkomplex-spezifsicher Antikörper zur Reduktion des Nullwerts beim Nachweis von Antigen-spezifisch gebundenen Antikörpern einer bestimmten Immunglobulinklasse in Array-Testformaten |
| US20080206782A1 (en) * | 2005-03-08 | 2008-08-28 | Genentech, Inc. | Methods for Determining the Bivalency of Protein and Antibody Therapeutics |
| MX2009003562A (es) * | 2006-10-04 | 2009-04-15 | Genentech Inc | Ensayo elisa para la deteccion de vegf. |
| EP1917854A1 (en) | 2006-11-06 | 2008-05-07 | F. Hoffmann-La Roche Ag | Method for producing anti idiotypic antibodies |
| US8242247B2 (en) | 2007-12-21 | 2012-08-14 | Hoffmann-La Roche Inc. | Bivalent, bispecific antibodies |
| US9266967B2 (en) | 2007-12-21 | 2016-02-23 | Hoffmann-La Roche, Inc. | Bivalent, bispecific antibodies |
| US20090162359A1 (en) | 2007-12-21 | 2009-06-25 | Christian Klein | Bivalent, bispecific antibodies |
| US8227577B2 (en) | 2007-12-21 | 2012-07-24 | Hoffman-La Roche Inc. | Bivalent, bispecific antibodies |
| PE20120591A1 (es) | 2009-04-02 | 2012-05-23 | Roche Glycart Ag | Anticuerpos multiespecificos que comprenden anticuerpos de longitud completa y fragmentos fab de cadena sencilla |
| WO2010115589A1 (en) | 2009-04-07 | 2010-10-14 | Roche Glycart Ag | Trivalent, bispecific antibodies |
| MX2011011925A (es) | 2009-05-27 | 2011-12-06 | Hoffmann La Roche | Anticuerpos triespecificos o tetraespecificos. |
| US9676845B2 (en) | 2009-06-16 | 2017-06-13 | Hoffmann-La Roche, Inc. | Bispecific antigen binding proteins |
| US8703132B2 (en) | 2009-06-18 | 2014-04-22 | Hoffmann-La Roche, Inc. | Bispecific, tetravalent antigen binding proteins |
| CN102207054B (zh) | 2010-03-31 | 2013-03-13 | 比亚迪股份有限公司 | 一种混合动力车的爆震控制系统及方法 |
| CN102207504A (zh) * | 2011-03-23 | 2011-10-05 | 北京华创远航科技有限公司 | 一种酶联检测试剂盒及其制备方法 |
-
2012
- 2012-12-18 CA CA2853917A patent/CA2853917A1/en not_active Abandoned
- 2012-12-18 KR KR1020147016700A patent/KR20140099277A/ko not_active Ceased
- 2012-12-18 JP JP2014547933A patent/JP6203747B2/ja active Active
- 2012-12-18 MX MX2014007095A patent/MX349003B/es active IP Right Grant
- 2012-12-18 RU RU2014128510A patent/RU2633488C2/ru not_active IP Right Cessation
- 2012-12-18 CN CN201280063032.5A patent/CN104040350B/zh active Active
- 2012-12-18 BR BR112014011535A patent/BR112014011535A2/pt not_active Application Discontinuation
- 2012-12-18 EP EP12806045.6A patent/EP2795335B1/en active Active
- 2012-12-18 WO PCT/EP2012/075991 patent/WO2013092611A2/en not_active Ceased
-
2014
- 2014-06-18 US US14/308,539 patent/US9671394B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2795335A2 (en) | 2014-10-29 |
| BR112014011535A2 (pt) | 2017-05-09 |
| RU2633488C2 (ru) | 2017-10-12 |
| JP2015502547A (ja) | 2015-01-22 |
| KR20140099277A (ko) | 2014-08-11 |
| WO2013092611A3 (en) | 2013-10-17 |
| CN104040350B (zh) | 2016-05-18 |
| CN104040350A (zh) | 2014-09-10 |
| MX2014007095A (es) | 2014-07-22 |
| MX349003B (es) | 2017-07-06 |
| RU2014128510A (ru) | 2016-02-10 |
| US20150072359A1 (en) | 2015-03-12 |
| WO2013092611A2 (en) | 2013-06-27 |
| US9671394B2 (en) | 2017-06-06 |
| HK1201920A1 (zh) | 2015-09-11 |
| JP6203747B2 (ja) | 2017-09-27 |
| EP2795335B1 (en) | 2017-09-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20170929 |
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| FZDE | Dead |
Effective date: 20191218 |