CA2826056C - Two phase immiscible system for the pretreatment of embedded biological samples - Google Patents

Two phase immiscible system for the pretreatment of embedded biological samples Download PDF

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Publication number
CA2826056C
CA2826056C CA2826056A CA2826056A CA2826056C CA 2826056 C CA2826056 C CA 2826056C CA 2826056 A CA2826056 A CA 2826056A CA 2826056 A CA2826056 A CA 2826056A CA 2826056 C CA2826056 C CA 2826056C
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Prior art keywords
carrier composition
forming layer
biological sample
reagent forming
sample
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CA2826056A
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English (en)
French (fr)
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CA2826056A1 (en
Inventor
Steen Hauge Matthiesen
Soren Nielsen
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Agilent Technologies Inc
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Agilent Technologies Inc
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • G01N1/312Apparatus therefor for samples mounted on planar substrates
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36Embedding or analogous mounting of samples
    • G01N2001/366Moulds; Demoulding

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2826056A 2011-02-28 2012-02-28 Two phase immiscible system for the pretreatment of embedded biological samples Active CA2826056C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161447364P 2011-02-28 2011-02-28
US61/447,364 2011-02-28
PCT/IB2012/000405 WO2012117294A2 (en) 2011-02-28 2012-02-28 Two phase immiscible system for the pretreatment of embedded biological samples

Publications (2)

Publication Number Publication Date
CA2826056A1 CA2826056A1 (en) 2012-09-07
CA2826056C true CA2826056C (en) 2021-01-26

Family

ID=46758338

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2826056A Active CA2826056C (en) 2011-02-28 2012-02-28 Two phase immiscible system for the pretreatment of embedded biological samples

Country Status (7)

Country Link
US (1) US10295442B2 (enExample)
EP (1) EP2686662B1 (enExample)
JP (1) JP6013376B2 (enExample)
CN (2) CN107255584B (enExample)
AU (3) AU2012222963B2 (enExample)
CA (1) CA2826056C (enExample)
WO (1) WO2012117294A2 (enExample)

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CN109142012A (zh) * 2018-08-17 2019-01-04 上海迈景纳米科技有限公司 一种ffpe样本快速脱蜡装置及其使用方法
CN113785184B (zh) * 2019-05-14 2025-02-28 文塔纳医疗系统公司 包括生物学样品处理腔室的系统
CN110132673B (zh) * 2019-05-22 2021-06-15 上海市农业科学院 一种制备金针菇子实体组织切片的方法
CN112113822A (zh) * 2019-06-21 2020-12-22 深圳迈瑞生物医疗电子股份有限公司 生物样本染色装置、推片染色机及生物样本染色方法
CN111238912A (zh) * 2020-03-12 2020-06-05 河南农业大学 一种叶片切片包埋的方法和叶片制片方法
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CN113092219B (zh) * 2021-03-24 2025-07-18 迪帕微(上海)生物科技有限公司 一种硬组织切片防脱预处理装置及防脱预处理方法
US20230392196A1 (en) * 2022-06-06 2023-12-07 Applied Materials, Inc. RNA retrieval process for preparing formalin-fixed, paraffin-embedded (FFPE) tissue samples for in situ hybridization

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Also Published As

Publication number Publication date
WO2012117294A2 (en) 2012-09-07
EP2686662B1 (en) 2022-01-19
EP2686662A2 (en) 2014-01-22
US20140051118A1 (en) 2014-02-20
JP2014523514A (ja) 2014-09-11
WO2012117294A3 (en) 2016-07-28
AU2018250513A1 (en) 2018-11-15
CN107255584B (zh) 2021-04-13
CA2826056A1 (en) 2012-09-07
AU2018250513B2 (en) 2020-05-21
EP2686662A4 (en) 2017-07-26
CN103930761A (zh) 2014-07-16
CN107255584A (zh) 2017-10-17
AU2012222963B2 (en) 2016-09-22
AU2012222963A1 (en) 2013-08-22
AU2016222358A1 (en) 2016-09-22
JP6013376B2 (ja) 2016-10-25
US10295442B2 (en) 2019-05-21
CN103930761B (zh) 2017-06-13

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